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   粪样 在 畜牧与动物医学 分类中 的翻译结果: 查询用时:0.021秒
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粪样
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  fecal sample
    PCR and denaturing gradient gel electrophoresis(DGGE) were used to track the development of fecal bacterial community of 5 diarrhea piglets. Clone libraries were created from complete 16S rDNA of 42-day fecal sample of piglet 1,2 and 5. From the library,clones had their V6-V8 regions matched predominant bands on the DGGE gel were then sequenced and their 16S rDNAs subjected to an online similarity search.
    用PCR/DGGE技术跟踪一窝5头新生腹泻仔猪自然康复、补饲、断奶过程中粪样细菌区系的演变,构建3头仔猪42日龄粪样的16S rDNA克隆库,分析匹配于DGGE优势谱带23个克隆的16S rDNA序列。
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  “粪样”译为未确定词的双语例句
    Detection of Canine Coronavirus in Dog Feces by ELISA
    ELISA法检测犬腹泻粪样中的犬冠状病毒
短句来源
    Mebendazole was used to feed the positive animal at the dosage of 5mg/kg·BW with food at one time.
    用甲苯咪唑按5mg/kg体重的剂量随食物经口一次投服,用药5d后检查粪样,虫卵完全转阴。
短句来源
    Detection of Enterotoxin Gene in Feces of Piglets with ETEC Diarrhea
    仔猪粪样中大肠杆菌肠毒素基因的检测
短句来源
    Detection of two porcine coronavirus in 158 shares of clinical samples by multiple RT-PCR
    应用多重RT-PCR方法检测158例猪粪样中的两种冠状病毒
短句来源
    Tracking of the development of fecal bacterial community of diarrhea piglets by 16S rDNA techniques
    16S rDNA技术研究新生腹泻仔猪粪样细菌区系的多样性变化
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  fecal sample
The fecal sample was extracted with acetonitrile, defatted with hexane, cleaned-up using C18 cartridge.
      
Efficacy was assessed by analyzing the reduction in fecal egg counts of a fecal sample taken between days 7 to 1 before treatment in comparison to a fecal sample taken between days 7 to 13 after treatment.
      
Genomic DNA was extracted directly from fecal sample of each animal and nested PCR was performed to amplify part of the 18S ribosomal RNA (18SrRNA) of the Cryptosporidium species.
      
Somatic coliphage concentrations in feces of feral pigeons were typically low with an occasional fecal sample having high numbers of either one of the two types of phages seen in this population of birds.
      
By contrast, the number of fruit species per gorilla fecal sample (average 0.8 species) was much lower than that for gorillas in the lowland habitats.
      
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The object of this research is to make sure of the application of acid insoluble ash(AIA)as indicator to the dairy goat dagestion trials. Six Xi Nong Saanen dairy goats were selected and tests were made in specially constructed metabolism cages. The means and standard deviations of the recoveries of natural indicators SiO_and AIA(concentrated HCl,4N HCl and 2N HCl)in the goatf ecals are 97.9±10.5%,96.7±1.96,100.7±1.23% and 105.7±1.98% respectively.It is therefore concluded that only the mean recovery of 4N HCl-AIA...

The object of this research is to make sure of the application of acid insoluble ash(AIA)as indicator to the dairy goat dagestion trials. Six Xi Nong Saanen dairy goats were selected and tests were made in specially constructed metabolism cages. The means and standard deviations of the recoveries of natural indicators SiO_and AIA(concentrated HCl,4N HCl and 2N HCl)in the goatf ecals are 97.9±10.5%,96.7±1.96,100.7±1.23% and 105.7±1.98% respectively.It is therefore concluded that only the mean recovery of 4N HCl-AIA is not significantly different(P>0.05)from 100%.The digestibility of dry matter, crude ash,gross energy,ether extract,crude fiber,crude protein and nitrogen- free-extract in ration determined by SiO_2 and AIA indicator mentioned above showed that only all the values determined by the method of 4N HCl-AIA near (P>0.05)the traditional method of collecting totol fecal.After quantitative feeding,the fecal samples were collected and the 2N HCl-AIA content dairy was analysed for two weeks.From ihe 6th day on,constant(P>0.05)value determined daily appeared.The 2N HCl-AIA contents of the fecal samples in different fecal collec-tion period were also determined,and the mixed samples of the 6th,7th,and 8th days showed no significant difference(P>0.05). On the 15th day after quantitativa feeding the fecal samples were collected at interval of 4-hour a day(6 times ewery day)and analysed.The 4N HCl-AIA contents of the fecal samples collected at any 4-hour interval showed no signiticant difference(P>0.05)from the whole fecal samplescollected in the same day.

试验旨在研究酸不溶灰分(Aeid Insoluble Ash,缩写为 AIA)在奶山羊消化试验中的应用。选择西农莎能奶山羊六只,在特制代谢笼内试验。试验测得内指示剂 SiO_2 及 AIA(浓 HCl、4NHCl 和2NHCl)在粪中的回收率分别为97.9±1.05%、96.7±1.96%、100.7±1.23%和105.7±1.98%。用 SiO_2 及三种 AIA 为指示剂测定日粮中干物质、粗灰分、总能、醚浸出物、粗纤维、粗蛋白质及无氮浸出物的消化率,除 4N HCl-AIA 法的各项测值接近于(P>0.05)传统的全粪收集法外,其它指示剂法均有一定差异。定量饲喂后,连续两个星期,逐日收集粪样,并分析其 2NHCl-AIA 含量,结果指出;从第六日始,各日测值趋于稳定(P>0.05)。试验测定了收粪期长短不同的粪样中2NHCl-AIA 含量,结果:6天、7天和8天的混合粪样差异不显著(P>0.05).于定量饲喂后的第15天,每隔4小时收集粪样一次,并分析之,结果:一天内任一4小时所采不完全粪样的4NHCl-AIA 含量同该日全粪收集样本未见有明显...

试验旨在研究酸不溶灰分(Aeid Insoluble Ash,缩写为 AIA)在奶山羊消化试验中的应用。选择西农莎能奶山羊六只,在特制代谢笼内试验。试验测得内指示剂 SiO_2 及 AIA(浓 HCl、4NHCl 和2NHCl)在粪中的回收率分别为97.9±1.05%、96.7±1.96%、100.7±1.23%和105.7±1.98%。用 SiO_2 及三种 AIA 为指示剂测定日粮中干物质、粗灰分、总能、醚浸出物、粗纤维、粗蛋白质及无氮浸出物的消化率,除 4N HCl-AIA 法的各项测值接近于(P>0.05)传统的全粪收集法外,其它指示剂法均有一定差异。定量饲喂后,连续两个星期,逐日收集粪样,并分析其 2NHCl-AIA 含量,结果指出;从第六日始,各日测值趋于稳定(P>0.05)。试验测定了收粪期长短不同的粪样中2NHCl-AIA 含量,结果:6天、7天和8天的混合粪样差异不显著(P>0.05).于定量饲喂后的第15天,每隔4小时收集粪样一次,并分析之,结果:一天内任一4小时所采不完全粪样的4NHCl-AIA 含量同该日全粪收集样本未见有明显差异(P>0.05)。

An indirect hemagglutination inhibition test ( IHAI ) has been develo

用纯化A型魏氏梭菌毒素致敏醛化绵羊红细胞,建立了检测A型魏氏梭菌毒素的间接血凝抑制试验(IHAI)。此法可检出20ng/ml的A型魏氏梭菌毒素,比小白鼠致死性测定法的敏感性高约13倍;与小鼠中和试验(NT)检测家兔粪样有很高的符合率。间接血凝抑制试验具有快速、敏感、特异简便和易于推广等优点,可用于家兔A型魏氏梭菌下痢的快速诊断和其它方面的毒素检测。

A strain of porcine rotavirus isolated from the feces of diarrheal piglets in Jiangsu province was replicated serially in MA104 cell cultures.The genome, pathogenicity and antigenicity of the cell-cultures of different passages were Studied. Same variation in the PAGE pattern of genomic RNA from the advanced passages of cell cultures were noticed. The pathogenicity of the virus became weaker gradually, the cell culture of the 90 th passage almost lost its pathogenicity entirely assh-own by the fact that neither...

A strain of porcine rotavirus isolated from the feces of diarrheal piglets in Jiangsu province was replicated serially in MA104 cell cultures.The genome, pathogenicity and antigenicity of the cell-cultures of different passages were Studied. Same variation in the PAGE pattern of genomic RNA from the advanced passages of cell cultures were noticed. The pathogenicity of the virus became weaker gradually, the cell culture of the 90 th passage almost lost its pathogenicity entirely assh-own by the fact that neither diarrhea nor pathologic changes were induced in the newborn piglets fed with the culture, and it remained safe or nonpathogenic even it was passed through the alimentary canal of newborn piglets three times successively. The 90th passage virus remained its antigenicity as shown in the detection of the viral hemagglutination test and cross reaction with hyperimmune serum in agar diffusion test.

从腹泻仔猪粪样中分离出一株猪轮状病毒(Na 86毒株),经在MA104细胞上连续传代后,对其不同代次的细胞毒进行了核酸图谱的分析、毒力及抗原性测定。结果表明,随着培养代次的增高,病毒核酸图谱有所变化,同时对未吮初乳仔猪的致病力逐渐下降,传至82和90代的细胞毒已完全失去了致病性,它既不引起仔猪腹泻也未使肠道组织发生病理变化,在未吮初乳猪肠道内连传三代,未见毒力反强;通过血凝特性的测定及高免血清的交互琼脂扩散试验等证明90代病毒,仍具有原来的良好抗原性。

 
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