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结肠菌群
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  colonic microflora
     Objective: To study the relationship between colonic microflora and lactose intolerance symptoms.
     目的 : 探讨结肠菌群构成与乳糖不耐受 (lactose intolerance,LI)症状之间的关系 ,以及饮奶对结肠双歧杆菌数量的影响。
短句来源
     Studies on fluorescence in situ hybridization with group-specific 16S rRAN-based probes in analysis of human colonic microflora
     荧光原位杂交技术分析人结肠菌群方法研究
短句来源
     Objective To study the effects of probiotics and yoghurt supplementation on colonic microflora in subjects with lactose intolerance (LI).
     目的研究补充益生菌和酸奶对乳糖不耐者结肠菌群的影响。
短句来源
     In order to establish the optional experimental conditions of fluorescence in situ hybridization (FISH) for analysis of colonic microflora, and analyze the stability and validation of the technique, fresh fecal samples were collected from the volunteers and five group-specific 16S rRNA-targeted oligonucleotide probes were used, and the effects of keeping time and temperature of fresh fecal samples after collected, the centrifugal speed and time, the storage time of paraformaldehyde-stocks of samples on the results were analyzed.
     建立荧光原位杂交技术分析人体内结肠菌群的方法。 取受试者新鲜粪便 ,选用 5种特异性的 16SrRNA寡核苷酸探针 ,检测粪便样本收集后的保存时间、温度 ,离心条件及样本固定液存放时间对杂交计数结果的影响。
短句来源
     Effect of probiotics and yogurt on colonic microflora in subjects with lactose intolerance
     益生菌和酸奶对乳糖不耐受者结肠菌群作用研究
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  colon flora
     EFFECT OF OAT β-GLUCAN ON COLON FLORA AND ITS FUNCTION IN MICE
     燕麦β-葡聚糖对小鼠结肠菌群及其功能的影响
短句来源
     Objective: To investigate the effect of oatβ-glucan on colon flora and its function in mice.
     目的:研究燕麦β-葡聚糖对小鼠结肠菌群及其功能的影响。
短句来源
  “结肠菌群”译为未确定词的双语例句
     Absorption and distribution of5-aminosalicylic acid from its chitosan capsul e degraded by colon bacteria-released enzymes in rats
     结肠菌群触发型5-氨基水杨酸壳聚糖胶囊大鼠体内的吸收与分布研究
短句来源
     . Statistical analysis showed that treatment with S1 had no marked effect on diversity index of predominant bacteria from hindguts in piglets.
     统计分析表明,仔猪口服益生菌S1对其盲肠和结肠菌群的多样性指数无显著影响。
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  colonic microflora
The carbohydrate crystalean and colonic microflora modulate expression of glutathione S-transferase subunits in colon of rats
      
The effect of short-term cefoxitin prophylaxis on the colonic microflora in patients undergoing colorectal surgery
      
The colonic microflora returned to normal after two weeks in all patients and no new colonizing cefoxitin-resistant bacteria were isolated.
      
Further studies are needed to establish whether alterations in the metabolism of the colonic microflora can reduce the risk of large bowel cancer in humans.
      
The effect of piperacillin prophylaxis on the colonic microflora in patients undergoing colorectal surgery
      
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  colon flora
Effect of bacampicillin on human mouth, throat and colon flora
      
Erythromycin caused only minor changes in the saliva flora while the aerobic and anaerobic colon flora were considerably disturbed.
      
Clindamycin depressed both the anaerobic saliva and colon flora.
      
If the colon flora Cat's Claw Monograph Copyright 1998 James B.
      
  colon bacteria
Inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by Bifidobacterium adolesce
      
In vitro studies have shown that active constituents of ginger inhibit multiplication of colon bacteria.
      
Preliminary studies to transform colon bacteria to express plant and animal genes were conducted.
      


In order to establish the optional experimental conditions of fluorescence in situ hybridization (FISH) for analysis of colonic microflora, and analyze the stability and validation of the technique, fresh fecal samples were collected from the volunteers and five group-specific 16S rRNA-targeted oligonucleotide probes were used, and the effects of keeping time and temperature of fresh fecal samples after collected, the centrifugal speed and time, the storage time of paraformaldehyde-stocks of samples on the results...

In order to establish the optional experimental conditions of fluorescence in situ hybridization (FISH) for analysis of colonic microflora, and analyze the stability and validation of the technique, fresh fecal samples were collected from the volunteers and five group-specific 16S rRNA-targeted oligonucleotide probes were used, and the effects of keeping time and temperature of fresh fecal samples after collected, the centrifugal speed and time, the storage time of paraformaldehyde-stocks of samples on the results were analyzed. The stability and the validation were assessed by CV values. Results showed that: (1) The optional experimental conditions were that the fresh fecal samples were not kept for more than 12 hours at 4℃, the centrifugal speed of samples was 700g for 2 minutes, and the paraformaldehyde-stocks were not kept for more than 5 mooths at low temperature (-80℃) before analysis; (2) The method had good stability and could detect the differences of colonic microflora composition between objects at a significant level.

建立荧光原位杂交技术分析人体内结肠菌群的方法。取受试者新鲜粪便 ,选用 5种特异性的 16SrRNA寡核苷酸探针 ,检测粪便样本收集后的保存时间、温度 ,离心条件及样本固定液存放时间对杂交计数结果的影响。结果建立最佳实验条件为 :粪便样本收集后应尽快在 4℃下保存 ,放置时间不要超过 12小时即作处理 ;样本的适宜离心条件为 70 0g 2分钟 ;样本用多聚甲醛固定后在 - 80℃下存放时间不要超过 5个月。该方法具有较好的稳定性 ,可以有效地检出个体之间结肠菌群的差异。

Objective: To study the relationship between colonic microflora and lactose intolerance symptoms. Methods: 42 volunteers were chosen and divided into lactose intolerance(LI) and lactose malabsorption groups according to the 25 g lactose-challenge test and symptoms questionnaires. Fresh fecal samples were collected from the volunteers, and the fluorescence in situ hybridization (FISH) technique with group-specific 16S rRNA-targeted oligonucleotide probes was used to quantify the total bacterial and predominant...

Objective: To study the relationship between colonic microflora and lactose intolerance symptoms. Methods: 42 volunteers were chosen and divided into lactose intolerance(LI) and lactose malabsorption groups according to the 25 g lactose-challenge test and symptoms questionnaires. Fresh fecal samples were collected from the volunteers, and the fluorescence in situ hybridization (FISH) technique with group-specific 16S rRNA-targeted oligonucleotide probes was used to quantify the total bacterial and predominant groups of bacteria in fecal samples. The effect of milk-drinking habit on the number of Bifidobacteria was analyzed. Results: It showed that there was significant difference in the number of total bacteria and Bifidobacteria between lactose intolerance groups and malabsorption group(P<0.05). A significantly negative correlation was found between the individual intolerance symptom scores and the number of total bacteria(P<0.05). Compared with 0~2 times/week and 0 times/week milk-drinking groups, the number of Bifidobacteria was significantly higher in the 5~7 times/week milk-drinking group(P<0.05). Conclusion: The occurrence of lactose intoler ance symptoms might be related to the amount of total bacteria and Bifidobacteria in the colon, and the milk-drinking habit could influence the number of Bifidobacteria.

目的 : 探讨结肠菌群构成与乳糖不耐受 (lactose intolerance,LI)症状之间的关系 ,以及饮奶对结肠双歧杆菌数量的影响。方法 : 根据乳糖负荷试验 ,结合问卷调查筛选成人乳糖吸收不良者、轻度及重度乳糖不耐受者作为试验对象。收集受试者一次新鲜全便 ,选用 5种特异性的 1 6S r RNA寡核苷酸探针 ,应用荧光原位杂交技术检测结肠细菌总数以及几种主要菌属 (种 )的构成。结果 : 乳糖不耐受组与吸收不良组相比 ,细菌总数和双歧杆菌数有显著性差异 (P<0 .0 5) ,细菌总数与乳糖不耐受症状评分之间呈负相关 (P<0 .0 5) ;每次饮奶以平均 2 0 0 ml计 5~ 7次 / w饮奶组与 0~ 2次 / w饮奶组或 0次 / w饮奶组相比 ,双歧杆菌数量显著增高 (P<0 .0 5)。结论 : 结肠细菌总数和双歧杆菌数可能与 LI症状的产生有关 ,同时饮奶习惯可能对双歧杆菌数量有潜在影响。

Objective To investigate the dynamic variability of intestinal flora and endotoxins in rats with fulminate hepatic failure. Methods Establishing the fulminate hepatic failure models by intraperitoneal injection of Galactosamine. Forty Sprague-Dawley rats were divided into three groups: group A (n = 10) were killed at the beginning of the experiment as control; while Group B (n = 12) and C (n = 18), the fulminate hepatic failure models, were killed 24 and 48 hours respectively after successful induction. Then,...

Objective To investigate the dynamic variability of intestinal flora and endotoxins in rats with fulminate hepatic failure. Methods Establishing the fulminate hepatic failure models by intraperitoneal injection of Galactosamine. Forty Sprague-Dawley rats were divided into three groups: group A (n = 10) were killed at the beginning of the experiment as control; while Group B (n = 12) and C (n = 18), the fulminate hepatic failure models, were killed 24 and 48 hours respectively after successful induction. Then, the contents of the jejunum, ileum and colon descendents were collected and a quantitative analysis was made about intestinal flora. Meanwhile, the concentrations of endotoxin in portal vein and right ventricle were determined and so were those in contents of ileums and colons. Results Our experiments showed that the livers of rats in group B were injured most seriously among three groups, and a minor recovery of hepatic function was observed in group C with the decrease of total bile acids (P < 0.05). Analysis on intestinal flora show: the intestiral enterobacteriacea increase and the lactobacillus decrease in group B (P < 0.01 in jejunum and ileum and P < 0.05 in colon). The comparisons between group C and B showed that the enterobacteriacea in the former decreased in both jejunum and colon (P < 0.05) while the number of lactobacillus recovered in the jejunum of group C (P < 0.05). Quantitative analysis on endotoxins showed that the ileum endotoxin increased in group B (P < 0.05) and in group C, endotoxins in ileam and colons also increased (vs. control, P < 0.01); portal endotoxin in group B showed higher level . than that in group A and C (P < 0.01). Conclusions The alteration of intestinal flora was observed in fulminate hepatic failure rats. Abnormal intestinal flora might lead to incline of endotoxin in ileum, colon and portal vein, while the recovery of normal intestinal flora would decrease the level of portal endotoxin.

目的 研究急性肝衰竭大鼠肠道菌群及内毒素的动态变化。 方法 腹腔注射半乳糖胺建立急性肝衰竭大鼠模型。40只SD大鼠随机分为A组(对照组)10只;B组12只,C组18只(均为肝衰竭大鼠)。实验开始时(A组)、造模后24 h(B组)和48 h(C组)分别处死各组大鼠并检测肝功能,定性、定量分析空肠、回肠及结肠菌群,定量测定门静脉、右心室血,回肠及结肠内毒素。 结果 肝功能检测显示:B组大鼠的肝脏损伤最为严重;与B组相比,C组大鼠的肝功能开始好转。肠道菌群分析显示:B组大鼠肠道内肠杆菌科细菌显著增加(空肠、回肠间,P<0.01;结肠间,P<0.05)、乳酸杆菌下降(P<0.01);与B组相比,C组空肠和结肠内的肠杆菌科细菌出现下降(P<0.05)、乳酸杆菌增加,以空肠为显著(P<0.05)。内毒素测定表明B组回肠内毒素增加(P<0.05);C组空肠和回肠内毒素继续增高与对照组差异有显著性(P<0.01);门静脉内内毒素在B组最高,与A、C两组比较差异有显著性(P<0.01)。 结论 急性肝衰竭大鼠肠道存在菌群失调、肠杆菌科细菌过度生长,菌群失调程度与肝损伤程度有关;肠道菌群失调伴有回肠和结肠内内毒素升高...

目的 研究急性肝衰竭大鼠肠道菌群及内毒素的动态变化。 方法 腹腔注射半乳糖胺建立急性肝衰竭大鼠模型。40只SD大鼠随机分为A组(对照组)10只;B组12只,C组18只(均为肝衰竭大鼠)。实验开始时(A组)、造模后24 h(B组)和48 h(C组)分别处死各组大鼠并检测肝功能,定性、定量分析空肠、回肠及结肠菌群,定量测定门静脉、右心室血,回肠及结肠内毒素。 结果 肝功能检测显示:B组大鼠的肝脏损伤最为严重;与B组相比,C组大鼠的肝功能开始好转。肠道菌群分析显示:B组大鼠肠道内肠杆菌科细菌显著增加(空肠、回肠间,P<0.01;结肠间,P<0.05)、乳酸杆菌下降(P<0.01);与B组相比,C组空肠和结肠内的肠杆菌科细菌出现下降(P<0.05)、乳酸杆菌增加,以空肠为显著(P<0.05)。内毒素测定表明B组回肠内毒素增加(P<0.05);C组空肠和回肠内毒素继续增高与对照组差异有显著性(P<0.01);门静脉内内毒素在B组最高,与A、C两组比较差异有显著性(P<0.01)。 结论 急性肝衰竭大鼠肠道存在菌群失调、肠杆菌科细菌过度生长,菌群失调程度与肝损伤程度有关;肠道菌群失调伴有回肠和结肠内内毒素升高;门静脉内毒素的增加与肠道菌群失调有关

 
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