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Cercospora oryzae Miyake is the pathogen of the narrow brown leaf spot of rice. Twenty four isolates were screened by the bioassay of root growth inhibition for toxin production. The majority of culture filtrates exhibited the inhibitory effect on the radicle growth of rice seeds. Six isolates: I-16, I-26, I-28, I-38, I-42 and I-49 were selected for further study. Potato sucrose broth plus 10% rice juice (PSBE) favored isolate growth and toxin production. The ranges of optimum temperature and pH for growth were... Cercospora oryzae Miyake is the pathogen of the narrow brown leaf spot of rice. Twenty four isolates were screened by the bioassay of root growth inhibition for toxin production. The majority of culture filtrates exhibited the inhibitory effect on the radicle growth of rice seeds. Six isolates: I-16, I-26, I-28, I-38, I-42 and I-49 were selected for further study. Potato sucrose broth plus 10% rice juice (PSBE) favored isolate growth and toxin production. The ranges of optimum temperature and pH for growth were 25—30℃ and pH 6—7 respectively. Light and aeration could stimulate growth. Temperature, light and aeration had no effect on the toxicity of the culture filtrates. The culture filtrates from culture at pH 6—7 showed the highest toxicity to radicle growth. The highest toxicity of culture filtrates occurred on the third week after inoculation, while the growth peak for most of isolates were on the fourth week. Partial characterization of the toxin was undertaken. The results illustrated that 6 isolates were capable of producing red pigment and yellow substance. Bed pigment was identified as cercosporin by thin layer chromatography, visible spectrum analysis and color reaction. Culture filtrates and cercosporin solution could inhibit the radicle growth on the seeds of different rice varieties and 4 crops (mungbean, sorghum, cowpea and corn). It also could induce chlorosis and necrosis on the injured leaf surface of rice seedings regardless of the varietal resistance, susceptibility, and seedling age. 水稻尾孢霉(Cercospora oryzae)是水稻条叶枯病的致病菌。24个菌株用抑制稻种胚根生长生物测定法进行产毒筛选。大部分菌株培养滤液对胚根生长有抑制作用。6个菌株:I-16,I-26,I-28,I-38,I-42和I-49选为进一步试验的菌株。添加10%稻叶汁马铃薯蔗糖培养液适于菌株的生长和产毒。生长适宜的温度和pH范围分别是25°-30℃和pH 6—7,光线和通气可促进菌株生长,但温度、光线和通气对培养滤液的毒性无影响,pH6—7的培养滤液毒性最强。接种后3周的培养滤液表现强毒性。多数菌株生长高峰出现在第4周。对水稻尾孢霉毒素进行了初步鉴定。结果表明菌株都能产生红色色素和黄色物质,红色色素经薄层色谱,可见光谱分析和颜色反应证明与尾孢霉毒素相同。培养滤液和尾孢霉毒素提取物能抑制稻种和4种作物种子胚根生长,并能在损伤稻叶上引起褪绿和枯死。这一作用与稻秧的品种抗性和秧龄无关。 Alternaria solani is a pathogen that causes the early blight of tomato and potato.One hundred thirty five isolares were tested by the bioassay of tomato radicle growth inhibition for their toxigenicity.The results showed that the isolate ucltures differed in their toxicity significantly.According to their inhibitory effect on the radicle growth,the isolates could be divided into five groups(Ⅰ-Ⅴ), whose inhibitory effect on the radicle growth was 14.7%,26.0%,34.7%,45.0% and 68.7%,respectively.In addition,there... Alternaria solani is a pathogen that causes the early blight of tomato and potato.One hundred thirty five isolares were tested by the bioassay of tomato radicle growth inhibition for their toxigenicity.The results showed that the isolate ucltures differed in their toxicity significantly.According to their inhibitory effect on the radicle growth,the isolates could be divided into five groups(Ⅰ-Ⅴ), whose inhibitory effect on the radicle growth was 14.7%,26.0%,34.7%,45.0% and 68.7%,respectively.In addition,there was statistically significant difference in the toxicity between filtrate extract and mycelia extract whose inhibitory effect was 50.6% and 42.7%,respectively,due to the different toxins in the two extracts. Although the four strongly toxigenic isolates,No.28,51,77 and 89 differed in their mycelial dry weight,crude yield,and fluorescent intensity,their effect on the radicle growth showed no significant difference. 茄病交链孢霉(Alternaria solani)是番茄和马铃薯早疫病的致病菌。135个菌株经抑制番茄胚根生长测定,表明菌株培养物毒性差异显著,按其对胚根生长的抑制程度可分成五个菌组。菌组对胚根生长的平均作用分别为14.7%,26.0%,34.7%,45.0%和68.7%。此外培养滤液和菌丝两种提取物对胚根生长的作用分别为50.6%和42.7%(统计上差异显著),这是由于两者存在不同毒素所致。菌株 No.28,55,71和89的菌丝干重和粗毒素产量有差异,但生物作用无显著不同,是可作进一步研究的菌株。 Twenty isolates of Piricularia oryzae cultured in potato sucrose solution were tested for toxicity of their culture filtrates by bioassay of rice radicle growth inhibition. The results showed the significant difference intoxicity among the culture filtrates. According to the inhibltory effect of culture filtrates on the radicle growth, the isolates could be divided into 4 groups (Ⅰ—Ⅳ)whose inhibitory effects were 7.7%, 18.4%, 62.5%, 100% (radicle growth rate), respectively. In addition the toxicity of culture... Twenty isolates of Piricularia oryzae cultured in potato sucrose solution were tested for toxicity of their culture filtrates by bioassay of rice radicle growth inhibition. The results showed the significant difference intoxicity among the culture filtrates. According to the inhibltory effect of culture filtrates on the radicle growth, the isolates could be divided into 4 groups (Ⅰ—Ⅳ)whose inhibitory effects were 7.7%, 18.4%, 62.5%, 100% (radicle growth rate), respectively. In addition the toxicity of culture filtrates would decrease with the increase in mycelial growth and pigmentation for the toxigenic isolates. 1987—1988年间,作者测定了籼粳稻类的20个稻瘟病菌株培养液对水稻种子胚根的毒害。经显著性测定表明,各菌株间培养液的毒性差异显著。这种差异表现为菌液对胚根生长抑制的程度大小不同,将其分成四组,即毒性强、毒性中等、毒性弱和无毒性。各组的胚根平均生长率依次为7.7%,18.4%,62.5%和100%;各组菌株数占所测菌株数的百分率分别为15%,30%,40%和15%。此外,随着菌丝体生长量增加和培养液色素加深,各组毒性也随之降低,呈负相关。
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