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兔骨骼肌
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  rabbit skeletal muscle
     AFM study of ryanodine receptor/calcium release channel of rabbit skeletal muscle
     兔骨骼肌ryanodine受体/钙释放通道的AFM研究
短句来源
     Study of repair of bone defect using rabbit skeletal muscle stem cells transduced by AdrhBMP-2 gene
     BMP-2基因转染兔骨骼肌干细胞修复骨缺损实验研究
短句来源
     The molecular weight of corn pollen actin is same with that of rabbit skeletal muscle actin (42 kD).
     玉米花粉肌动蛋白与兔骨骼肌肌动蛋白具有相同的分子量(42KD)。
短句来源
     AIM:To determine the feasibility of rabbit skeletal muscle stem cells(RSMSCs) mediated by AdrhBMP 2 gene as seed cells and demineralized bone matrix(DBM) as a scaffold material to construct tissue engineered artificial bone for the heter otopic bone formation in muscles of nude mice.
     目的:探索AdrhBMP-2修饰的兔骨骼肌干细胞(rabbitskeletalmusclestemcells,RSMSCs)作为种子细胞、脱钙骨基质Demineralizedbone(matrix,DBM)作为支架材料构建组织工程化人工骨裸鼠肌内异位成骨的可行性。
短句来源
     The effects of two oxidants, 1,4 naphthoquinone and glutathione (GSSG) on the initial binding rate of associate ryanodine binding the ryanodine sensitive calcium channel from rabbit skeletal muscle sarcoplasmic reticulum were examined by kinetic ryanodine binding method.
     采用ryanodine 动态结合方法,考察了1,4 萘醌(1,4NQ) 和谷胱甘肽(GSSG) 作用于兔骨骼肌肌质网(SR)ryanodine 敏感钙通道(RyR) 所产生的对ryanodine 初结合率(R0) 的影响。
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  rabbit skeletal muscles
     Chapter I Extraction, purification, identification and physicochemical properties of myosin from rabbit skeletal muscles: myosin was extracted, purified and identified from male rabbit(3 months old and had weight of 2~2.5kg) Pasoas major (PM) and Semimembranosus proprius (SMp) skeletal muscles.
     第1章 兔骨骼肌肌球蛋白提纯、鉴定及理化性质:从体重2~2.5kg的3月龄新西兰公兔的兔腰大肌(Pasoas major,PM)和半膜肌(Semimembranosus proprius,SMp)中提取、纯化、鉴定肌球蛋白。 肌球蛋白提取率为1%。
短句来源
     Methods TnC-Sepharose 4B affinity chromatography column was prepared with rabbit skeletal muscles troponin C(sInC) and used for the direct purification of cTn Ⅰ from human cardiac muscle tissue.
     方法 用兔骨骼肌Tnc(sTnC)制备TnC Sepharose 4B亲和柱 ,直接从心肌组织中分离提纯人cTnⅠ。
短句来源
     Methods Rabbit skeletal muscles troponin C(sTnC) was purified by ion exchange, gel filtration and hydrophobic interaction chromatography to prepare ThC-Sepharose 4B affinity chromatography column.
     方法 采用离子交换、分子筛、疏水 层析相结合的方法分离纯化兔骨骼肌肌钙蛋白C(sTnC).制备TnG-Sepharose4B亲和层析柱。
短句来源
     Study on the turbidity and solubility of myosin solution from rabbit skeletal muscles
     兔骨骼肌肌球蛋白溶液浊度和溶解度研究
短句来源
     Study on Heat-induced Gelation Properties and Gel Forming Mechanism of Myosin from Rabbit Skeletal Muscles
     兔骨骼肌肌球蛋白热诱导凝胶特性及成胶机制研究
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     ① The level of IL- 8 in the skeletal muscle was significantly higher in the rabbits with c hronic quadriceps injury than in the normal rabbits[(408 ± 13) ng/L vs (40± 26 ) ng/L,t=30.962, P=0.00].
     ①股四头肌慢性损伤模型兔骨骼肌中白细胞介素8水平显著高于正常兔[(408±13)和(40±26)ng/L,t=30.962,P=0.00]。
短句来源
     The specific activeties of the purified isoenzymes are 461μ/mg, 405μ/mg,and 506μ/mg, and their purities are 95.5%,89.1% and 95.1% respectively.
     从经5′-AMP-QT_4亲和层析纯化的兔骨骼肌LDH中分离出LDH-5,比活力为506μ/mg,纯度为95.1%。
短句来源
     Study on delayed protection of ischemia preconditioning for reperfusion injury of skeletal muscle in limbs of rabbits
     缺血预处理对兔骨骼肌再灌注损伤延迟保护作用的实验研究
短句来源
     Osteoinductivity of recombinent human bone morphogenetic protein-2 gene mediated with cationic liposome transfer in skeletal muscle stem cells of rabbits in vitro
     阳离子脂质体介导重组人骨形态发生蛋白-2基因转染的兔骨骼肌干细胞体外成骨分化的实验研究
短句来源
     RESULTS:The irreversible time limit of skeletal muscle in ischemic rabbits was 50 hours at 4 ℃while 28 hours at 6 ℃.
     结果:4℃时缺血兔骨骼肌的不可逆变性时限为50h; 6℃时缺血兔骨骼肌的不可逆变性时限为28h。
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  rabbit skeletal muscle
Kinetic analysis of the glycogen chain growth reaction catalyzed by glycogen phosphorylase b from rabbit skeletal muscle has been carried out over a wide range of AMP concentration under the saturation of the enzyme by glycogen.
      
Kinetics of Denaturation of Rabbit Skeletal Muscle Glycogen Phosphorylase b by Guanidine Hydrochloride
      
The effects of the osmolytes trimethylamine-N-oxide (TMAO), betaine, proline, and glycine on the kinetics of inactivation and aggregation of rabbit skeletal muscle glycogen phosphorylase b by guanidine hydrochloride (GuHCl) have been studied.
      
Interaction of phosphorylase kinase from rabbit skeletal muscle with flavin adenine dinucleotide
      
The interaction of flavin adenine dinucleotide (FAD) with rabbit skeletal muscle phosphorylase kinase has been studied.
      
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  rabbit skeletal muscles
Self-association of phosphorylase kinase from rabbit skeletal muscles under conditions close to those of an intracellular enviro
      
The size of the fibre populations in rabbit skeletal muscles as revealed by indirect immunofluorescence with anti-myosin sera
      
Lactate dehydrogenase isozymes in type I, IIA and IIB fibres of rabbit skeletal muscles
      
A quantitative histochemical analysis of almost 200 mouse triceps surae muscle fibres is presented, together with some data from similar surveys of rabbit skeletal muscles.
      
Early postnatal changes (4-5 days to 15 days after birth) in the biochemical composition of microsomes were investigated in rabbit skeletal muscles destined to become fast-twitch muscles.
      
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Although the occurence of a coenzyme I-independant, particle-bound α-glycerophosphate dehydrogenase in skeletal muscle of higher animals has long been recognized, little is known about its relation to the cytochrome system. Green has found that it is linked to cytochrome c but details of the electron transporting pathway has remained obscure. This problem has now been studied using the method of simultaneous action of two or more enzyme systems as described previously. Enzyme preparation obtained from thoroughly...

Although the occurence of a coenzyme I-independant, particle-bound α-glycerophosphate dehydrogenase in skeletal muscle of higher animals has long been recognized, little is known about its relation to the cytochrome system. Green has found that it is linked to cytochrome c but details of the electron transporting pathway has remained obscure. This problem has now been studied using the method of simultaneous action of two or more enzyme systems as described previously. Enzyme preparation obtained from thoroughly washed rabbit muscle mince has been employed in the present investigation. It has been found that in the presence of the rabbit muscle enzyme preparation, succinate and α-glycerophosphate each interferes with the rate of oxidation of the other when they are oxidized simultaneously. The inhibition of α-glycerophosphate oxidase by succinate can be reversed by the addition of pyrophosphate, a powerful inhibitor of succinic dehydrogenase. With cytochrome c as electron acceptor, the overall rate of simultaneous oxidation of α-glycerophosphate, succinate and reduced coenzyme I (CoIH) does not represent the sum of the rates of their separate oxidation, but corresponds only to the highest of the three rates, i.e. the rate of oxidation of CoIH. It is, therefore, believed that the α-glycerophosphate-, succinate- and CoIH-cytochrome c reductase systems have a common, velocity limiting electron carrier which is most probably the linking factor first proposed by Slater. In agreement with this conclusion, the α-glycerophosphate oxidase of rabbit muscle preparation has been found to be sensitive to the action of 2,3-dimercaptopropanol. Using 2,6-dichlorophenolindophenol, as acceptor, the overall rate of the simultaneous oxidation of succinate and α-glycerophosphate equals exacdy to the sum of the rates of their separate oxidation. Similar results have also been obtained even in presence of phenylurethane, which markedly inhibits the activity of succinic dehydrogenase and does not affect the activity of α-glycerophosphate dehydrogenase. These facts suggest that cytochrome b is not involved in the oxidation of α-glycerophosphate in rabbit muscle preparation. The pathway of hydrogen or electron transfer of the particulate α-glycerophosphate oxidase system may, therefore, be represented as follow: (See also Fig. 4)

(一) 在經徹底冲洗的兔骨骼肌製劑中,[L-α]甘油磷酸和琥珀酸的氧化彼此干涉。琥珀酸對[L-α]甘油磷酸氧化的抑制作用能因加入抑制琥珀酸脫氫酶的焦磷酸而解除。 (二) 當用細胞色素c作受體時[L-α]甘油磷酸,還原輔酶I和琥珀酸三者同時氧化時總氧化速度僅相當其中氧化速度最高者即還原輔酶I單獨氧化的速度。[L-α]甘油磷酸氧化酶系也因[2,3]二氫硫基丙醇的處理而失效。 (三) 當用[2,6]二氯酚靛酚作受體時[L-α]甘油磷酸和琥珀酸同時氧化時速度完全等於二底料單獨氧化時速度的和。[L-α]甘油磷酸的氧化不受苯代氨甲酸乙酯的影響。 (四) 本文結果說明[L-α]甘油磷酸的氧化不通過細胞色素b而通過中間因子和細胞色素c連接。

In this paper, effects of N-ethyl perhexiline (N-EP), a new drug synthesized in China, on calcium sequestration and Ca~(2+)-ATPase activity in skeletal muscle sareoplasmic reticulum vesicles (SR) were reported. N-EP (64~256μmol/L) inhibited SR calcium sequestration (12μg/mL SR protein); calcium content of SR decreased to 24% of control value at 128μmol/L N-EP. N-EP (8-128μmol/L) also inhibited SR Ca~(2+)-ATPase activity (6μg/mL SR protein), which dropped to 20% of control value at 128μmol/L N-EP, and the degree...

In this paper, effects of N-ethyl perhexiline (N-EP), a new drug synthesized in China, on calcium sequestration and Ca~(2+)-ATPase activity in skeletal muscle sareoplasmic reticulum vesicles (SR) were reported. N-EP (64~256μmol/L) inhibited SR calcium sequestration (12μg/mL SR protein); calcium content of SR decreased to 24% of control value at 128μmol/L N-EP. N-EP (8-128μmol/L) also inhibited SR Ca~(2+)-ATPase activity (6μg/mL SR protein), which dropped to 20% of control value at 128μmol/L N-EP, and the degree of inhibition depended on the ratio of N-EP to SR membrane protein. Inhibition of calcium transport by N-EP may be related to calcium antagonism. No effect of "7921", puerarin and daidzein on enzyme activity was found.

本文报道国内合成新药N-乙基沛心达(N-EP)对兔骨骼肌肌浆网囊泡钙螯合作用和钙-ATP酶(Ca~(2+)-ATPase)活力的抑制作用以及药物与膜蛋白的比例变化对后者的影响。N-EP抑制钙转运可能与其钙拮抗作用有关。“7921”、葛根素及黄豆甙原对该酶活力无明显影响。

Ca2 + -ATPase from sarcoplasmic reticulum was reconstituted into the soybean phospholipid vesicles with the freeze-thawing mathod.Four types of proteoliposomes containing sarcoplasmic reticulum Ca2+-ATPase have been prepared; Ca2+-"free" ,L.(Ca2+-ATPase) ;outside Ca2+-containing, L.(Ca2+-ATPase) ; inside Ca2+-containing,L. (Ca2+-ATPase) and two-side Ca2+-containing,L.(Ca2+-ATPase) proteoliposomes. L.(Ca2+-ATPase) was prepared by ATP driven Ca2+ pumping from outside into the interior of the proteoliposomes. Being...

Ca2 + -ATPase from sarcoplasmic reticulum was reconstituted into the soybean phospholipid vesicles with the freeze-thawing mathod.Four types of proteoliposomes containing sarcoplasmic reticulum Ca2+-ATPase have been prepared; Ca2+-"free" ,L.(Ca2+-ATPase) ;outside Ca2+-containing, L.(Ca2+-ATPase) ; inside Ca2+-containing,L. (Ca2+-ATPase) and two-side Ca2+-containing,L.(Ca2+-ATPase) proteoliposomes. L.(Ca2+-ATPase) was prepared by ATP driven Ca2+ pumping from outside into the interior of the proteoliposomes. Being chelated by EGTA the outside Ca2+ of the vesicles, the L. (Ca2+-ATPase) was transformed into the L. (Ca2+ -ATPase) .The fluidity of the proteoliposomes has been compared by fluorescence polarization probes diphenylhexatriene (DPH) or 7-, 12-, 16-(9-anthroyloxy)stearic acid (7, 12, 16-AS). The degree of polarization in these proteoliposomes decreases in the order.L.(Ca2+-ATPase) >L.(Ca2+-ATPase) >L.(Ca2+-ATPase) >L.(Ca2+-ATPase) .Concomitantly with the lipid fluidity measurements the ATP driven Ca2+ transport and Ca2+-ATPase activity were also monitored. It was interested to note that as the proteoliposomes were loaded with Ca2+ due to ATP driven Ca2+ pumping, the reconstituted Ca2+-ATPase activity as well as the Ca2+ transport was gradually inhibited. The ATP hydrolysis rate of Ca2+-ATPase containing proteoliposomes in the 4th minute was only 9% of that in the 1st minute. However, no similar inhibition was observed when the activity of purified non-incorporating Ca2+-ATPase was determined under the same experimental conditions. So, it seems that the reconstituted Ca2+-ATPase activity in liposomes may be modulated by the Ca2+-mediated changing fluidity of the lipid bilayer, resulting from the variation of transmembrane Ca2+ concentration gradient.

重建在大豆磷脂脂质体上的兔骨骼肌肌质网Ca~(2+)—ATP酶在ATP驱动下可将溶液中的Ca~(2+)转运到脂酶体内部;外加EGTA则可除去脂酶体外部的Ca~(2+),由此可得到四种含Ca~(2+)状态不同的脂酶体:(1)内、外都无Ca~(2+);(2)仅外部有Ca~(2+);(3)内、外都有Ca~(2+);(4),仅内部有Ca~(2+).用DPH和AS系列萤光探针对这四种含Ca~+状态不同的脂酶体的膜脂流动性进行了测定,结果表明:脂酶体外部加入Ca~(2+),脂双层外表面的流动性降低.当Ca~(2+)进入脂酶体内部后,内表面膜脂的流动性也降低,而且外层膜脂流动性进一步降低.脂酶体内、外的Ca~(2+)含量不同时,Ca~(2+)—ATP酶功能状态也不同.转运到脂酶体内部的ca~(2+)积累到一定浓度后,通过Ca~(2+)泵向内转运的Ca~(2+)及Ca~(2+)—ATP酶活力都受到了抑制.转运进行到第四分钟时的酶活只有第一分钟的9%.但在相同的实验条件下,失去了完整的膜结构的纯化的Ca~(2+)—ATP酶蛋白没有被抑制.这提示完整的膜结构是这种抑制作用所必需的,而且膜两侧Ca~(2+)浓度的梯差可...

重建在大豆磷脂脂质体上的兔骨骼肌肌质网Ca~(2+)—ATP酶在ATP驱动下可将溶液中的Ca~(2+)转运到脂酶体内部;外加EGTA则可除去脂酶体外部的Ca~(2+),由此可得到四种含Ca~(2+)状态不同的脂酶体:(1)内、外都无Ca~(2+);(2)仅外部有Ca~(2+);(3)内、外都有Ca~(2+);(4),仅内部有Ca~(2+).用DPH和AS系列萤光探针对这四种含Ca~+状态不同的脂酶体的膜脂流动性进行了测定,结果表明:脂酶体外部加入Ca~(2+),脂双层外表面的流动性降低.当Ca~(2+)进入脂酶体内部后,内表面膜脂的流动性也降低,而且外层膜脂流动性进一步降低.脂酶体内、外的Ca~(2+)含量不同时,Ca~(2+)—ATP酶功能状态也不同.转运到脂酶体内部的ca~(2+)积累到一定浓度后,通过Ca~(2+)泵向内转运的Ca~(2+)及Ca~(2+)—ATP酶活力都受到了抑制.转运进行到第四分钟时的酶活只有第一分钟的9%.但在相同的实验条件下,失去了完整的膜结构的纯化的Ca~(2+)—ATP酶蛋白没有被抑制.这提示完整的膜结构是这种抑制作用所必需的,而且膜两侧Ca~(2+)浓度的梯差可通过影响膜脂来调节Ca~(2+)—ATP酶的功能.

 
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