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前脑啡肽
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  proenkephalin
     Expression of Proenkephalin Gene in NIH3T3 Cells
     前脑啡肽原基因在NIH3T3细胞的表达
短句来源
     Effect of scorpion venom on the expression of proenkephalin in C57BL/6 mice with Parkinson disease
     蝎毒对C57BL/6帕金森病小鼠脑内前脑啡肽原表达的影响
短句来源
     METHODS:Complete Freund's adjuvant was used to induce adjuvant arthritis(AA)rats,observed pain threshold,pro-opiomelanocortin(POMC)and proenkephalin(PENK)mRNA in inflamed location at 1h,3h,6h,12h,24h,and 48h after electro-acupuncture analgesia.
     方法:以Freunds完全佐剂造成AA大鼠模型,在电针治疗后1h、3h、6h、12h、24h和48h观测大鼠痛阈、炎症局部前阿黑皮素(POMC)和前脑啡肽(PENK)mRNA的表达。
短句来源
     The expression of proenkephalin(PENK) and prodynorphin(PDYN) mRNA were detected by RT-PCR.
     RT-PCR法检测小鼠右侧纹状体内前脑啡肽(proenkephalin,PENK)和前强啡肽(prodynorphin,PDYN)mRNA的表达。
短句来源
     The proenkephalin mRNA expression in hippocampus on the 10th and 21st day after birth was significantly lower in chronic pain group than in control group ( P < 0.05).
     在出生后第10天和第21天,疼痛组大鼠海马前脑啡肽mRNA表达均低于对照组(P<0.05)。
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  “前脑啡肽”译为未确定词的双语例句
     Methods In situ hybridization techniques were used to evaluate the influence of NMDA receptor antagonist MK-801(5-methyl-10,11-dihydro-5H-dibenzocycloheptan-5,10-iminemaleate) and non-NMDA receptor antagonist DNQX(6,7-dinitroquinoxaline-2,3-dione)microinjected into the hippocampus on preproenkephalin(PPE)mRNA and preprodynorphin(PPD)mRNA expression during penicillin induced epilepsy.
     方法应用原位杂交方法观察了青霉素致及海马内微量注射NMDA受体阻断剂MK-801(5-methy1-10,11-dihydro-5H-dibenzo[a,d]cycloheptan-5,10-iminemaleate)和非NMDA受体阻断剂DNQX(6,7-dinitroquinoxaline-2,3-dione)后,大鼠海马内前脑啡肽原和前强啡肽原mRNA表达的变化。
短句来源
     AIM: To detect different expression of pre-proenkephalin mRNA (PPE mRNA) in 16-wk-old spontaneously hypertensive rat (SHR) and age-matched normotensive Wistar-Kyoto rat ( WKY) .
     目的:检测前脑啡肽原信使RNA(PPE mRNA)在16周自发性高血压大鼠(SHR)和Wistar-Kyoto大鼠(WKY)中的不同表达。
短句来源
     The effects of muscarinic and NMDA receptor antagonist on the expression of preproenkephalin and preprodynorphin mRNA in spinal cord and brain stem during morphine withdrawal in rats
     毒蕈碱和NMDA受体拮抗剂对吗啡依赖大鼠脊髓和脑干前脑啡肽原和前强啡肽原mRNA表达的影响
短句来源
     ELECTROACUPUNCTURE ENHANCES ENKEPHALIN mRNA EXPRESSION IN THE SPINAL CORD AND MEDULLA, AN IN SITU HYBRIDIZATION STUDY
     电针可促进前脑啡肽原mRNA在大鼠脊髓和延髓的表达:原位杂交研究
短句来源
     ④Compared with the saline treated control group,the expression of PENK mRNA in the encephalic region of mice of the model group significantly increased (P<0.05).
     ④与盐水对照组相比,模型组小鼠脑区前脑啡肽原mRNA表达明显增强(P<0.05)。
短句来源
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  相似匹配句对
     Expression of Proenkephalin Gene in NIH3T3 Cells
     脑啡肽原基因在NIH3T3细胞的表达
短句来源
     Expression of human preproenkephalin gene in somatic cell line cells
     人脑啡肽原基因在体细胞系细胞的表达
短句来源
     RADIOIMMUNOASSAY FOR ENKEPHALINS
     脑啡肽的放射免疫测定
短句来源
     THE PREPARATION OF ANTI-LEU-ENKEPHALIN SERUM AND ANTI-MET-ENKEPHALIN SERUM
     脑啡肽抗血清的制备
短句来源
     Conclusions Anterior acromioplasty has been verified as effective and safe surgical procedure for shoulder impingement syndrome.
     术ASE
短句来源
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  proenkephalin
Elevated proenkephalin-derived peptide levels in ACTH-producing adenomas
      
In this work, we studied the processing of proenkephalin-derived peptides in postmortem human pituitary (PMHP), ACTH-producing adenomas (ACTH-PA), nonfunctioning adenomas (NFA), and GH-producing adenomas (GH-PA).
      
ACTH-PA contained at least 10 times more proenkephalin-derived peptides than PMHP, NFA, and GH-PA.
      
Proenkephalin processing was different in the four tested tissues.
      
In ACTH-PA, proenkephalin was processed to high-, intermediate-, and low-mol-wt products.
      
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cDNA probes encoding preproenkephalin A (PPA) were used to detect the mRNA codings within the sections of adrenal glands of guinea pigs, processing according to the following procedures; perfused the animals with Zambonis' or 4% paraformaldehyde fixative; rinsed the block of adrenal glands for 12 hours in O.1mol/L phosphate sodium solution; the block were cut at -15℃ in a cryostat; Then the sections were prehybridized and hybridized with the biotinylated cDNA encoding PPA. The sections were incubated in the...

cDNA probes encoding preproenkephalin A (PPA) were used to detect the mRNA codings within the sections of adrenal glands of guinea pigs, processing according to the following procedures; perfused the animals with Zambonis' or 4% paraformaldehyde fixative; rinsed the block of adrenal glands for 12 hours in O.1mol/L phosphate sodium solution; the block were cut at -15℃ in a cryostat; Then the sections were prehybridized and hybridized with the biotinylated cDNA encoding PPA. The sections were incubated in the solution containing streptavi- din-biotinylated HRP and chromostained with DAB. The positive reactive produ- cts appeared in the cytoplasm of about 90% of adrenal medullary cells.

取经Zambonis’固定液固定的豚鼠肾上腺作冰冻切片.切片经预杂交后,用含生物素标记的前脑啡肽原A(PPA)的cDNA探针的杂交液孵育,然后孵于链霉抗生物素-辣根过氧化物酶复合物溶液中,用DAB呈色.结果:约90%的肾上腺髓质细胞呈阳性反应,阳性反应产物位于细胞质中。

Previous studies in this laboratory have shown that electroacupuncture (EA) accelerated the release of enkephalin in the spinal cord. The present study was undertaken toinvestigate the effect of EA stimulation on the expression of preproenkephalin (PPE)mRNA in the rat spinal cord and medulla by in situ hybridization histochemical technique. Animals were administrated with 2 Hz EA stimulation (1-2-3 mA, 30 min) ap-plied at acupoints sanyingjiao and zusanli of one hind leg. The rats were perfused 24 hafter EA,...

Previous studies in this laboratory have shown that electroacupuncture (EA) accelerated the release of enkephalin in the spinal cord. The present study was undertaken toinvestigate the effect of EA stimulation on the expression of preproenkephalin (PPE)mRNA in the rat spinal cord and medulla by in situ hybridization histochemical technique. Animals were administrated with 2 Hz EA stimulation (1-2-3 mA, 30 min) ap-plied at acupoints sanyingjiao and zusanli of one hind leg. The rats were perfused 24 hafter EA, and quantitative changes of PPE-mRNA expression were determined by emul-sion autoradiography. EA stimulation was found to increase the number of neurons ex-pressing PPE-mRNA in spinal cord and medulla. Increased expression of PPE--mRNAwas more marked in ipsilateral dorsal horn of spinal cord (especially in laminae Ⅲ--Ⅳand contralateral ventromedial medulla (especially in the lateral paragigantocellularreticular nucleus). The results provide evidence in support of the enkephalinergic hy-pothesis of acupuncture analgesia. It is suggested that increased biosynthesis ofenkephalin precursor would help to compensate for the loss of tissue storage ofenkephalin during the period of EA stimulation.

本实验室以往的研究表明电针可促进脊髓脑啡肽释放,本研究进一步利用原位杂交方法观察电针后前脑啡肽原mRNA在脊髓和延髓的表达。结果表明电针刺激(2Hz,1-2-3mA,30)后24h同侧脊髓背角前脑啡肽原(PPE)-mRNA阳性细胞数高于对侧。电针后对侧延髓腹内侧网状结构[包括延髓网状巨细胞核(Gi)及其α部(GiA)和Gi的外侧旁核(LPGi)]PPE-mRNA阳性细胞数高于同侧。我们推测电针诱发前脑啡肽原mRNA表达增加可弥补因脑啡肽释放增多而导致脑啡肽前体物质的损失,从而参与针刺镇痛的长期效应。

In this study, a non-isotopic in situ hybridization histochemistry (ISHH) techniques were used to observe the expression of preproenkephalin(PPE) mRNA in the rat brain following electroacupnncture (EA). The probe for PPE mRNA was labeled with digoxigenin(DIG). Ten hours following EA, the signal intensity and area with PPE mRNA in neurons were increased significantly in many pain or analgesia-related nuclei, such as caudate putamen, accumbens, preoptic area, midbrain regions including periaqueductal gray(PAG),...

In this study, a non-isotopic in situ hybridization histochemistry (ISHH) techniques were used to observe the expression of preproenkephalin(PPE) mRNA in the rat brain following electroacupnncture (EA). The probe for PPE mRNA was labeled with digoxigenin(DIG). Ten hours following EA, the signal intensity and area with PPE mRNA in neurons were increased significantly in many pain or analgesia-related nuclei, such as caudate putamen, accumbens, preoptic area, midbrain regions including periaqueductal gray(PAG), interpeduncular nucleus, suhstantia nigra, red nucleus etc. Previous studies have shown that EA promoted the release of enkephalin in the brain, which may result in the loss of enkephalin precursor. The resultS in the present study suggest that EA-induced enhancement of PPE mRNA expression in the rat brain may be the compensation to the loss of enkephalin precursor and underlied the mechanisms of the long-term and accumulating effect of acupuncture analgesia(AA).

本文采用原位杂交组织化学技术,结合计算机图象处理系统,对电针后大鼠脑内前脑啡肽原(PPE)mRNA的表达进行半定量的观察。PPEmRNA探针以半抗原的地高辛标记。电针10小时之后,PPEmRNA阳性信号的强度和神经元内合PPEmRNA的面积在许多与痛和镇痛有关的核团明显增加,如尾核,伏核,视前区,中脑的脚间核,导水管周围灰质,黑质,红核等。表明电针促进了PPEmRNA在大鼠脑内的表达。这可能是针刺具有累积和长期效应的机制之一。

 
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