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j链
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  j chain
     Expression of α-defensin-1 with J chain in the transfected COS-7 cells
     带J链的α-防御素-1基因转染COS-7细胞后的表达检测
短句来源
     J chain and IgA(d) are produced by local activated B cells, while SC is synthesized by mu-cosal epithelial cells.
     J链和IgA(d)由局部活化B细胞产生,SC由黏膜上皮细胞合成;
短句来源
     Reconstruction of J Chain Gene with HNP-1 Gene and Construction of Its Expression Vector
     人α-防御素-1基因与J链基因的融合及其表达载体的构建
短句来源
     (Methods) J chain and HNP1 cDNA were amplified from the plasmids pCHJ and pBabeNeoHNP1(respectively),then the JHNP1 DNA fragment was obtained by recombinant polymerase chain reaction(PCR).
     方法采用聚合酶链反应(PCR)方法分别从pCH J质粒和pB abeN eo HNP 1质粒中扩增出J链和HNP 1;
短句来源
     Methods J chain cDNA from pCH plasmid was connected to HNP-1 cDNA from pBabeNeo plasmid by PCR, then the recombinant cDNA was inserted into the expression vector pcDNA3.1(-)/Myc-HisC.
     方法 将从pCH质粒中获取的J链片段,采用人工连接方法与HNP 1片断相连接,并将其构建于新型哺乳动物细胞质粒表达载体pcDNA3 .1( ) Myc HisC中,进行序列测定。
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  “j链”译为未确定词的双语例句
     Recombinant of J chain-HNP-1 cDNA and the construction of expression vector
     带J链的α-防御素-1的基因重组和表达载体的构建
短句来源
     It was known that the molecular weight of heavy chains, light chains and joining chains were about 64~65ku, 26~28ku and 16ku respectively from Sodium sulphate polyacrylamide get electrophoresie (SDS-PAGE).
     经SDS—聚丙烯酰胺凝胶电泳(SDS-PAGE)可知,鸡SIgA重链分子质量为64~65ku,轻链为26~28ku,J链约16ku;
短句来源
     METHODS The J-HNP-1 cDNA fragments were produced by recombinant PCR.
     方法利用重组PCR技术,使HNP-1一端带上J链;
短句来源
     Sodium sulphate polyacrylamide gel electrophoresis indicated that the molecular weight of heavy chains,light chains and joining chains were about 63~64 ku,26~28 ku and 16 ku,respectively.
     SDS聚-丙烯酰胺凝胶电泳检测表明,重链分子质量为63~64 ku,轻链为26~28 ku,J链约16 ku。
短句来源
     2. The J-HNP-1 recombinant was inserted into the mammalian express vector pcDNA3.1(-)/Myc-HisC successfully, double label gene Myc and 6XHis were in the downstream.
     1.利用重组PCR技术使J链与HNP-1这两个不同的基因相连接而成为J-HNP-1重组体。
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  相似匹配句对
     Chain of Immunology
     免疫
短句来源
     CHAIN OF TESTS OF SERIES
     判别
短句来源
     Completeness of Jordan-Chain
     Jordan的完备性
短句来源
     Jones Polynomial of Links
     环Jones多项式
短句来源
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  j chain
Secreted polymeric immunoglobulins, IgA dimers and IgM pentamers, are unique in having not only the L and H chains but also the J chain, which is responsible for oligomerization.
      
The content of the J chain and the transcription level of its gene were studied in normal and tumor cells at various stages of squamous cell cancer and adenocarcinoma of the lungs by RT-PCR and immunoblotting.
      
Fractionally charged excitations in the charge density wave state of a quarter-filled t-J chain with quantum phonons
      
The J chain is a component of polymeric immunoglobulin (Ig) molecules and may play an important role in their polymerization and the transport of polymeric Ig across epithelial cells.
      
In this study, the primary structure of the chicken J chain was determined by sequencing cDNA clones.
      
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Six lines of hybridoma secreting antibody against the μ chain of human IgM were pro- duced with the conventional hybridoma technique.In ELISA assay,these McAbs specifi- cally reacted with IgM,and no cross reaction was observed with heavy,light or J chain of other immunoglobulins.Only a 72 kD polypeptide was recognized by these antibodies,as estimated by Southern-blotting.Radioimmunoassay indicated that these antibodies recognized 4 different antigenic determinants of the μ chain.The use of these antibodies in...

Six lines of hybridoma secreting antibody against the μ chain of human IgM were pro- duced with the conventional hybridoma technique.In ELISA assay,these McAbs specifi- cally reacted with IgM,and no cross reaction was observed with heavy,light or J chain of other immunoglobulins.Only a 72 kD polypeptide was recognized by these antibodies,as estimated by Southern-blotting.Radioimmunoassay indicated that these antibodies recognized 4 different antigenic determinants of the μ chain.The use of these antibodies in studying human B cell differentiation and detecting specific IgM to infectious pathogens such as HBc in the sera of patients with infectious diseases was also discussed.

本实验用可溶性抗原人 IgM 免疫 BALB/C 小鼠,按常规方法取免疫鼠脾细胞,分别与 NS-1、SP2/0细胞进行融合,ELISA 法筛选只与 IgM 反应阳性的抗体分泌杂交细胞,建立了6株杂交瘤株。经鉴定,此6株细胞分泌的抗体与 IgM 产生特异性反应,而不与其它免疫球蛋白重、轻链及 J 链交叉反应。经免疫电转印的硝酸纤维膜染色只显示分子量为72kD 的电泳带。放射免疫方法检测6个单克隆抗体,可分别识别4个不同的抗原决定簇。此组抗体识别 B 细胞胞浆内及膜表面μ链并可检测乙型病毒性肝炎患者血清中 HB_C-IgM 复合物。本组抗体间彼此加合可提高检测的灵敏性。抗 IgM(μ链)单克隆抗体对研究人 B 细胞的分化、成熟及功能提供了重要途径。

:In this paper we research properties of B-chains of mocules over A- G. rings,and give the grade number,pure condition of B-chains and the relations between B-chains and J-chains,pure modules,holonomic modules,generalize the corresponding results.

该文进一步探讨了B-子模链的性质,给出了B-子模链的grade number,pure性,以及J-链,pure模,holonomic模与B-链的关系.

Aphageantibody (Fab)againstHIV 1gp1 2 0V3loopwasclonedbyscreeningahumanimmunoglobulincom binatoriallibrary.TheV3 bindingactivityandspecificityoftheantibodywereassayedbyELISAandcompetitiveinhibi tionELISA .ComparisonoftheFdgeneandlightchaingenewiththatinKABATdatabaseshowedthattheFdbelonged toIgG1subclass.ThevariableregionofheavychainwasVHIsubgroup,derivedfromrearrangedgerm linegeneof DP88.Whilethelightchainbelongedtoκsubtype.ThevariableregionwasVLIVsubgroup,derivedfromthegerm line geneDPK2 2 .

以HIV1gp120V3环的合成环肽为抗原,从人的噬菌体抗体组合文库中,筛选出与HIV1V3肽具有结合活性的人源性噬菌体抗体(Fab段)。用ELISA测定其活性,竞争抑制实验证实了该噬菌体抗体的特异性。序列分析表明,重链基因为IgG1亚类,可变区属VHI亚组,与胚系基因DP88的同源性最高,D区为D33,J区为JH5;轻链为κ亚型,可变区属VLIV亚群,D区为DPK22,J链为JK4胚系。

 
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