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邻联茴香胺
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  o-dianisidine
     Activity of myeloperoxidase (kU/g)= ΔA460/(12×content of tissue enzyme÷3),ΔA460: the value of 1 minute absorbance (A) in 460 nm; 3: the total volume of reaction; 12: the A of 1 mg O-dianisidine dihydrochloride per mililiter provided by Sigma company;
     计算髓过氧化物酶活力(kU/g)=ΔA460/(12×组织酶量/3),ΔA460为460nm处吸光度1min增大的值,3为反应的总体积,12为Sigma公司规定的每毫升1mg邻联茴香胺的吸光度;
短句来源
     Studies on Oxidation of o-Dianisidine by Electrochemistry and Optically Transparent Thin-layer Spectro-electrochemistry
     邻联茴香胺的电化学和薄层光谱电化学研究
短句来源
     In this thesis, some derivatives of aniline such as fuchsin basic (FB), o-aminophenol (OAP); o-dianisidine(ODA) were selected as the studying objects. The study was involved in spectroscopy, electrochemistry and the interaction of FB with DNA;
     本论文选取含有苯胺结构的若干有机小分子(碱性品红、邻氨基酚、邻联茴香胺)作为对象,分别对碱性品红的光谱和电化学性质及与DNA的相互作用,聚邻氨基酚/Ni~(2+)膜的制备、电催化性质及DNA的固定,邻联茴香胺在铂网栅光透电极上的薄层光谱电化学性质进行了研究。
短句来源
     The myeloperoxidase activity in colonic tissue, reflecting neutrophil infiltration was assayed by O-dianisidine method.
     用邻联茴香胺法检测结肠组织中MPO活性以考察中性白细胞浸润程度。
短句来源
  o dianisidine
     The enzyme has a wide range of substrates in the presence of oxygen as the electron receptor, including 2,6 dimethoxyphenol, ABTS, guaiacol, caffeic acid, ferulic acid, o dianisidine.
     SDS PAGE分析发现 ,该酶分子量为 6 8kD ,薄层等电聚焦测得等电点为 3 5 .漆酶Ⅰ的底物范围较宽 ,以O2 为电子受体 ,可以氧化多种木素单体模型物 ,包括 2 ,6 二甲氧基酚 ,2 ,2′ 联氮 二 (3 乙基 苯并噻唑 6 磺酸 )(ABTS) ,愈创木酚 ,咖啡酸 ,阿魏酸和邻联茴香胺 .
短句来源
     Four voltammetric enzyme linked immunoassay systems are investigated,which use p phenylenediamine(PPD), m aminophenol(MAP), o dianisidine(ODA)and N,N diethy1 p phenylemediamine (DEPPDA) as electrochemical substrates for horseradish peroxidase(HRP).
     分别以对苯二胺、间氨基酚、邻联茴香胺和硫酸对氨基 N,N-二乙基苯胺为辣根过氧化物酶 ( HRP)的电化学底物 ,这四种物质在 HRP的催化下能够被 H2 O2 氧化生成具有电化学活性的酶催化反应产物 ,可以用线性扫描二阶导数极谱法进行检测。
短句来源
  “邻联茴香胺”译为未确定词的双语例句
     Detection of Four Important Plant Viruses with Voltammetric Enzyme Linked Immunoassay Based on ODA H 2O 2 HRP System
     邻联茴香胺-H_2O_2-辣根过氧化物酶伏安酶联免疫分析检测四种重要植物病毒
短句来源
     No peroxidase activity of this enzyme was detected when using dianisidine, DAB and phenylenediamine as electron donor.
     在以邻联茴香胺、邻苯二胺和二氨基联苯胺作为电子供体进行过氧化物酶的活性测定时发现,同工酶4不显示相应酶活。
短句来源
     Improved gel electrophoresis of ceruloplasmin detecting ceruloplasmin oxidase activity of human serum with O-Tolidine
     改进血清铜蓝蛋白凝胶电泳—血清铜蓝蛋白氧化酶活性的邻联茴香胺显示法
短句来源
     Kinetic property of glucose oxidase-peroxidase (horse radish)-o-dianisidine system was investigated with UV-Vis spectro-meter when enzymer and dye concentration were fixed, and glucose concentration range of apparent first order reaction was found in this system. Glucose concentration was determined by the absorbance of dye, error is low, and recovery can reach 97% ~103%
     本文运用紫外-可见光谱仪研究了葡萄糖氧化酶偶联辣根过氧化物酶-邻联茴香胺染料体系的催化动力学性质,通过控制酶和染料的含量,确定了反应呈假一级反应的葡萄糖浓度范围,在此反应体系中,葡萄糖浓度可以通过测定染料吸光度而获得,干扰小,回收率达97%~103%。
短句来源
     The oxidation of o _ dianisidine(ODA) has been investigated at platinum minigrid electrode by UV-Vis optically transparent thin _ layer electrochemistry technique. From the experimental results of thin _ layer voltammograms and UV-Vis spectro _ electrochemistry of ODA in pH 2.0 BR buffer, it was found that the oxidation of ODA is a two electron transfer reversible process. In addition, the formal potential V0′ and electron transfer number n were calculated from spectra at different potentials.
     采用电化学和薄层光谱电化学方法 ,以三电极系统在酸性介质中对邻联茴香胺(ODA)电氧化性质进行了研究 ,测得邻联茴香胺的克式量电位、电子转移数和扩散系数 ,实验结果表明 ,邻联茴香胺在铂网栅电极上的电氧化为可逆的两电子转移过程
短句来源
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  o-dianisidine
Catalytic constants of wheat seed peroxidase were determined in the reactions of o-dianisidine and ascorbic acid peroxidation.
      
Mechanisms of peroxidase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of s
      
Peroxidase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate (SDS), an anionic surfactant, was spectrophotometrically studied.
      
As spray reagent only o-dianisidine (saturated solution in glacial acetic acid) proved to be suitable.
      
Kinetics of oxidation of o-dianisidine by hydrogen peroxide in the presence of antibody complexes of iron(III) coproporphyrin
      
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  o dianisidine
We assessed first the peroxidase activity of the G501S product spectroscopically using o dianisidine as the substrate.
      
  o_dianisidine
We assessed first the peroxidase activity of the G501S product spectroscopically using o dianisidine as the substrate.
      


The experiments were carried out in ten baby pigs at the age of 15-20 days with body weight of 1.5-3kg.The origin of the vagal preganglionic fibers of stomach wall was investigated by means of HRP techniques.100-200 μl of HRP solution was injected into gastric wall of each of these pigs.After 3 days,these baby pigs were slaugh- tered,their brainstems were treated according to O-D method(Edw- ards'79),and the labelled cells were identified with frozen serial sections.The results obtained are as follows: 1.The...

The experiments were carried out in ten baby pigs at the age of 15-20 days with body weight of 1.5-3kg.The origin of the vagal preganglionic fibers of stomach wall was investigated by means of HRP techniques.100-200 μl of HRP solution was injected into gastric wall of each of these pigs.After 3 days,these baby pigs were slaugh- tered,their brainstems were treated according to O-D method(Edw- ards'79),and the labelled cells were identified with frozen serial sections.The results obtained are as follows: 1.The HRP labelled cells were found evehly in the dorsal vagal nuclei of both sides in spite of injection sites and there was no evidence of asymmetry in distribution of HRP labelled cells. 2.The HRP labelled cells were present throughout the rostrocaudal extent of the dorsal vagal nucleus except for the ventrolateral border.After the injection of HRP into the wall of pyloric part, the labelled cells were found mainly in the medial aspects of the nucleus, although a few stippled cells were found in the lateral portion. 3.The number of the total labelled cells of the dorsal vagal nucleus, 17±7.3% was in the rostral region,41.4±5.6% in the rostromiddle region;26.8±7.2% in the caudomiddle region;and 14.8±5.3% in the caudal region. 4.A few labelled cells were observed in the area subpostrema. This finding is similar to the results of cholinesterase method and electrophysiological studies.Caudal to the level of obex,the labelled cells were not found in the medial solitary nucleus. 5.Except for the cases of pyloric injection,a cluster of labelled cells were observed in the intermediolateral nucleus of the first cervical segment of spinal cord,and a fewer-stippled labelled cells were found in the area between the intermediolateral nucleus and the central gray of Cl.In addition,in 2 pigs,some labelled cells were observed in the gray dorsal commissural nucleus of Cl. These results show that the vast majority of the preganglionic vagal fibers innervating the stomach arise from the dorsal vagal nucleus throughout its whole length,and most of them arise from the rostromiddle part of the nucleus at level above the obex.In addition, a few of them arise from the area subpostrema,and from the interme- diolateral nucleus and dorsal commisural nucleus of first cervical segment.

用 HRP 法逆行追踪了到胃壁去的迷走神经节前纤维的起源。将7~10%HRP100~200微升分点注入仔猪的胃壁,将脑干连续冰冻切片用邻联茴香胺绿色反应成色,置明视野显微镜下观察了标记神经元的分布。1.不分注射部分,HRP 标记细胞在两侧背迷核中大致呈对称性分布,而无一侧优势的存在。2.HRP 注射于全胃壁,标记细胞分布于背迷核除前中区和后中区外侧缘以外的全长范围内,尾端可延伸到第一颈髓。幽门部注射的标记细胞分布状况基本同全胃组,但幽门部标记细胞主要沿着核的内侧部分布,且在核的尾端无标记。3.背迷核中标记细胞,以前中区最多,后区最少。4.在各例胃壁注射 HRP 后,在闩和闩以上的亚极后区有零星的标记细胞,这与胆碱酯酶法和电生理学方法的结果相一致,在闩以下的孤束内侧核尾侧半未见标记细胞。5.除三例幽门部注射外,在第一颈髓的中间外侧核出现成簇的标记细胞,在中间外侧核与中央管之间的地带也有零星标记细胞,有两例在灰质背连合核出现标记细胞。这个结果未见文献报导。以上结果表明支配胃的迷走神经节前纤维起源于背迷核的全长,而以核的前中区最多。亚极后区、第一颈髓的中间外侧核、背连合核也发出少量迷走节前纤维支配胃。6...

用 HRP 法逆行追踪了到胃壁去的迷走神经节前纤维的起源。将7~10%HRP100~200微升分点注入仔猪的胃壁,将脑干连续冰冻切片用邻联茴香胺绿色反应成色,置明视野显微镜下观察了标记神经元的分布。1.不分注射部分,HRP 标记细胞在两侧背迷核中大致呈对称性分布,而无一侧优势的存在。2.HRP 注射于全胃壁,标记细胞分布于背迷核除前中区和后中区外侧缘以外的全长范围内,尾端可延伸到第一颈髓。幽门部注射的标记细胞分布状况基本同全胃组,但幽门部标记细胞主要沿着核的内侧部分布,且在核的尾端无标记。3.背迷核中标记细胞,以前中区最多,后区最少。4.在各例胃壁注射 HRP 后,在闩和闩以上的亚极后区有零星的标记细胞,这与胆碱酯酶法和电生理学方法的结果相一致,在闩以下的孤束内侧核尾侧半未见标记细胞。5.除三例幽门部注射外,在第一颈髓的中间外侧核出现成簇的标记细胞,在中间外侧核与中央管之间的地带也有零星标记细胞,有两例在灰质背连合核出现标记细胞。这个结果未见文献报导。以上结果表明支配胃的迷走神经节前纤维起源于背迷核的全长,而以核的前中区最多。亚极后区、第一颈髓的中间外侧核、背连合核也发出少量迷走节前纤维支配胃。6.本文对背迷核的功能进行了讨论。

This paper reported the method of measuring glucose oxidase activeties by using 2,4—dichlorphenol instead of o-dianisidin. This method showed an ideal relativity and responsibility. The method is suitable to determine glucose oxidase activity in the proccess of fermentation and purification and it has satisfactory results.

测定葡萄糖氧化酶(GOD,E.C.1.1.3.4)最广泛使用的方法,是利用葡萄糖氧化酶—辣根过氧化物酶—邻联茴香胺偶联反应,用分光光度法测定;但邻联茴香胺是致癌物,有必要选择毒性较低、灵敏度较高的新染料代替之。国外曾有报道,邻联甲苯胺、邻甲苯胺及2,6-二氯苯酚等一些染料可作为邻联茴香胺的代用品,但前二种仍有致癌性。国内虽有利用GOD酶法、用4-氨基安替比林偶联酚测定血清中葡萄糖含量(Trinder反应)的报道,并指出Barham及Trinder1972年提出用2.4-二氯苯酚代替酚,灵敏度更高,但直接用以测定GOD活性的方法尚未见报道。本文根据Trinder反应原理,选用2,4-二氟苯酚和苯酚共同做为该偶联反应的氧供体,选择了最佳测定条件,并进行了方法学评价。

Angioarchiteture of the CNS were visualized with peroxidase cytochemistry in rat. This technique can successfully demonstrat that the endogenous peroxidase activity of red cells and granular leukocytes(especilly polymorphonuclear and eosinophilic)were excellent mar- kers for revealing CNS blood vessels at the light microscopic level when 3,3′-diaminobenzi- dine (DAB), benzidine dihydrochloride (BDHC), O-dianisidine (O-D) and tetramethyl- benidine(TMB) were used as chromogen. The four peroxidase cytochemical...

Angioarchiteture of the CNS were visualized with peroxidase cytochemistry in rat. This technique can successfully demonstrat that the endogenous peroxidase activity of red cells and granular leukocytes(especilly polymorphonuclear and eosinophilic)were excellent mar- kers for revealing CNS blood vessels at the light microscopic level when 3,3′-diaminobenzi- dine (DAB), benzidine dihydrochloride (BDHC), O-dianisidine (O-D) and tetramethyl- benidine(TMB) were used as chromogen. The four peroxidase cytochemical approches are useful for visualizing CNS microvasculature in nomal laboratory animals; the four approches have potential application to cerebrovascular studies of stroke, trauma, neoplasia and intra- cerebral grafts.

应用血细胞中内源性过氧化物酶将底物过氧化氢分解,产生原子氧,使无色联苯胺及其衍生物呈色,显示中枢神经系统微血管的原理,在辣根过氧化物酶(HRP)呈色技术启示下,确立了4种中枢神经系统微血管显示法,即二氨基联苯胺法(DAB)、二盐酸联苯胺法(BDHC)、邻-联茴香胺法(O-D)以及四甲基联苯胺法(TMB)。实验结果表明,这4种方法均能清晰地呈现中枢神经系统内的徽血管,4种方法的显示结果并无显著性差异(P>0.05)。这4种方法的建立将为中枢神经系统微血管解剖学研究,病理组织血管构筑研究以及中枢神经系统内移植物血管重建研究提供了重要的技术手段。

 
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