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dna分离
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  dna separation
     The water-soluble mixtures of polymers, poly(N,N-dimethylacrylamide) (PDMA)/polyvinylpyrrolidone (PVP), were synthesized and used in capillary electrophoresis (CE) for DNA separation.
     合成了一种水溶性聚合物 ,聚N ,N 二甲基丙烯酰胺 (PDMA)和聚乙烯吡咯烷酮体系 (PVP) ,并用于DNA分离 .
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     Methods The observation of morphological ch anges of neutrophils,FACScan flow cytometer and technique of DNA separation were used in this study.
     方法运用形态学观察、流式细胞仪FACS技术和DNA分离技术进行研究。
短句来源
     The medium showed single-base-pair DNA separation by capillary electrophoresis even with ultraviolet detector and uncoated capillary column.
     本体系粘度较小 ,筛分能力强 ,具备芯片毛细管DNA分离介质的潜力 .
短句来源
     The capillary electrophoresis using non-gel sieving matrices is one of the most important techniques for DNA separation. The uncrosslinked polymer solutions are generally used as sieving matrices.
     使用无胶筛分介质的毛细管电泳是最重要的DNA分离技术之一,通常使用无交联的高分子溶液作为无胶筛分介质。
短句来源
     The capillary electrophoresis(including capillary array electrophoresis——CAE and microchip electrophoresis——MCE) using non-gel sieving matrices is one of the most important techniques for DNA separation.
     使用无胶筛分介质的毛细管电泳(包括毛细管阵列电泳和微芯片电泳)是最重要的DNA分离技术之一。
短句来源
  dna isolation
     Plant DNA Isolation
     植物DNA分离
短句来源
     The Development of Nanoparticles on DNA Isolation and Purification
     纳米磁性粒子在DNA分离与纯化中的应用进展
短句来源
     Methods Genetic DNA was prepared from peripheral blood leukocytes using DNA Isolation Kits for Mammalian Blood. The complete coding region and exon-intron splice sites of CNGA1 gene were amplified with polymerase chain reaction (PCR).
     方法采集患者外周血,应用DNA分离试剂盒提取DNA,应用11对CNGA1基因引物进行聚合酶链反应(polymerasechainreaction,PCR),扩增CNGA1基因的全部编码区及内含子外显子的拼接区。
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     the DNA isolation and preparation of strains A, B and C of poplar canker was studied for the first time and the optimum produce condition was verified,and karyotype of the three strains was studied, the results as follows:
     首次对引起杨树水疱型溃疡病原菌菌株 A、B、C 进行了染色体 DNA 分离和制备,研究清其最佳产生条件,并对三菌株的染色体 DNA 的核型进行了研究。 结果表明:
短句来源
     A prerequisite to RAPD is the iso- lation of good quality nuclear DNA. Cotton leaves contain high levels of polyphenolic com- pounds that irreversibly interact with proteins and nucleic acids during DNA isolation.
     RAPD分析需要分离高质量的核DNA,棉花叶片中多酚类化合物含量高,在DNA分离过程中,它们跟核酸、蛋白质发生不可逆反应,从而影响了DNA的得率和纯度。
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  “dna分离”译为未确定词的双语例句
     And plasmid profiles were clear respectively on agarose gel (0.5%), which were named P1,P2, P3 and P4 by their molecular weights.
     在0.5%琼脂糖凝胶电泳中,质粒DNA分离相当清晰。 Bt 4.0718菌株含有4个大小不等的质粒,依据其分子量大小顺序依次编号为P_1、P_2、P_3和P_4。
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     Isolation of Microsatellite DNA from Endangered Tree Toona ciliata var. pubescens and Optimization of SSR Reaction System
     珍稀濒危树种毛红椿微卫星DNA分离及SSR反应体系优化
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     The results showed that there were differences in quantity and purity of HSV DNA extracted with these methods.
     同时对用三种方法提取的HSV DNA的量以及与细胞DNA分离程度的差别作了比较。
短句来源
     In this dissertation, DNA fragment designated RM10 was isolated from the chromosomal DNA of the halophilic archaea Halobacterium halobium by using the Escherichia coli promoter-probe vector pKK232-8 and was shown to confer promoter activity in Escherichia coli.
     利用大肠杆菌启动子探针载体pKK232-8,从极端嗜盐古生菌中基因组DNA分离得到一个能在真细菌域(大肠杆菌)中具有启动子活性的RM10 DNA片段。
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     Method for ctDNA Isolation and Purification of Sugarbeet
     甜菜叶绿体DNA分离纯化方法
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  dna separation
Emphasis will be placed on recent biological and biomedical developments and trends such as enzyme immobilization, cell isolation, protein purification, target drugs and DNA separation.
      
Detailed methods are described for marker production and for DNA separation by contour-clamped homogeneous electric field (CHEF) electrophoresis.
      
A collision model for DNA separation by capillary electrophoresis in dilute polymer solution
      
A theoretical description, based on chemical kinetics and electrochemistry, is given of DNA separation in dilute polymer solution by capillary electrophoresis.
      
DNA separation with low-viscosity sieving matrix on microfabricated polycarbonate microfluidic chips
      
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  dna isolation
falciparum, we developed genus- and species-specific primers for PCR diagnostics of malaria, as well as a one-step effective method of DNA isolation from Giemsa-Romanovsky-stained thick blood smears.
      
A Composite Polyaniline-Containing Silica Sorbent for DNA Isolation
      
Here, we report an improved DNA isolation protocol for moss filaments, which is fast, reliable, cheap, quick, and easy.
      
Because of this, the procedure of DNA isolation is a step o critical importance for the analysis of bottom sediments.
      
A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study.
      
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Degradative plasmids which contain some genomes characteristics of microbial degradation of some environmental pollutants are of large molecular weight and hence difficult to be isolated from chromosome. Unlike chromosomal DNA, plasmid is supercoiled in structure, which renatures rapidly after heat denaturation followed by quick cooling. Taking advantage of this kind of property, a modification of both McMaster's and Kado's methods has been devised and works successfully. Experimental results indicate that the...

Degradative plasmids which contain some genomes characteristics of microbial degradation of some environmental pollutants are of large molecular weight and hence difficult to be isolated from chromosome. Unlike chromosomal DNA, plasmid is supercoiled in structure, which renatures rapidly after heat denaturation followed by quick cooling. Taking advantage of this kind of property, a modification of both McMaster's and Kado's methods has been devised and works successfully. Experimental results indicate that the plasmids isolated by this method retain the transformational effect. It seems to be a useful and rapid method to study plasmid on microbial degradation of recalcitrant pollutants

控制微生物对某些环境污染物降解的质粒——降解性质粒,分子量较大,难于和染色体DNA分离。超螺旋质粒DNA在热变性后冷却即快速复性方面与染色体DNA不同。根据此性质,作者改进了McMaster的和Kado的方法,获得了良好效果。实验证明。经此法纯化的质粒具有转化活性,是研究化学污染物的降解性质粒的简便方法。

Highly repeated sequence of eukaryotic genomes can be separated by gelelectrophoresis after digestion of the DNA with restriction endonucleases.Highlyrepeated fragments have been separated by digestion of DNA from the Rhesusmonkey with Bam HI.The smallest fragment was then covalently joined with theplasmid pBR 322.After transformation into E.coli,three clones were obtained.ThepMBI clone was characterized by digestion with BamH I and Pst I and hybridiza-tion with highly repeated DNA from the Rhesus monkey.Its...

Highly repeated sequence of eukaryotic genomes can be separated by gelelectrophoresis after digestion of the DNA with restriction endonucleases.Highlyrepeated fragments have been separated by digestion of DNA from the Rhesusmonkey with Bam HI.The smallest fragment was then covalently joined with theplasmid pBR 322.After transformation into E.coli,three clones were obtained.ThepMBI clone was characterized by digestion with BamH I and Pst I and hybridiza-tion with highly repeated DNA from the Rhesus monkey.Its molecular size is about340b.p.

哺乳动物基因组中高重复顺序可用限制性内切酶消化DNA经用电泳分离获得,本文用限制性内切酶Bam HI消化恒河猴DNA分离得到一系列高重复片段。用其中最小片段与质粒pBR 322共价连接,转化到大肠杆菌后得到3个带有重组子的克隆。其中PMBI克隆用Bam HI,Pst I酶解,杂交鉴定,证明此克隆是含有重组的恒河猴高重复片段,此片段大小约为340碱基对。哺乳动物基因组相当繁杂,一般在10~9碱基对(b.p)以上,应用复性动力学方法可将其分成高重复顺序、中重复顺序及单拷贝顺序。高重复顺序在基因组中重复频率很高。可达百万次。它有许多家族,这些家族核苷酸顺序相近,但不相同,目前有证据表明,高重复顺序与基因表达及调控有关,与生物进化有关,而且还与DNA的复制及调控有关,因此具有重要的生理功用。为了得到纯的单一高重复顺序,我们应用分子克隆技术,建立了恒河猴高重复顺序DNA的分子克隆。

In this paper, the isolation, purification, characterization and restriction map of mtDNA from Candida tropicalis 79 arc reported. In the mtDNA extract, there are three forms: linear, open circular and closed circular, observed by electron microscope. The mtDNA of C. tropicalis 79 has a buoyant density of 1.691 g/cm in CsCl. Its melting temperature is 80℃. Restriction enzymes EcoRI, BamHI and PstI have 6, 5 and 3 sites on this mtDNA, respectively. According to the size of restriction fragments, the molecular...

In this paper, the isolation, purification, characterization and restriction map of mtDNA from Candida tropicalis 79 arc reported. In the mtDNA extract, there are three forms: linear, open circular and closed circular, observed by electron microscope. The mtDNA of C. tropicalis 79 has a buoyant density of 1.691 g/cm in CsCl. Its melting temperature is 80℃. Restriction enzymes EcoRI, BamHI and PstI have 6, 5 and 3 sites on this mtDNA, respectively. According to the size of restriction fragments, the molecular weight of mtDNA is 22.97 md. Simultanously, we established the restriction cleavage map of this DNA by double digestion.

本文报道了热带假丝酵母线粒体DNA的分离纯化、性质和酶切图。此线粒体DNA用电镜观察有线状、开环状和闭环状三种形状;在氯化铯中浮力密度为1.691g/cm~3;解链温度为80.0℃。限制性内切酶EcoRⅠ、BamHⅠ和PstⅠ在此线粒体DNA上分别有6、5和3个切点。根据酶切片段计算分子量为22.97×10~6道尔顿。同时,用双酶切方法得到此线粒体DNA的内切酶图谱。

 
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