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水貂肠炎
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  mink enteritis
     Diagnosis of Mink Enteritis Caused by Coronavirus
     冠状病毒感染引起水貂肠炎的诊断
短句来源
     BALB/c mice were immunized with cell culture preparation of the minkenteritis virus(MEV),and their splenocyte; were fused with NS-1 myeloma cells. 8 hy-bridoma cell lines secreting specific monoclonal antibodies against mink enteritis par-vovirus were produced after cloning by limiting dilution three times.
     以我国分离的水貂肠炎细小病毒(MEV)为抗原免疫BALB/c小鼠获得的脾细胞与NS-1骨髓瘤细胞融合,筛选出8株分泌抗MEV的单克隆抗体杂交瘤细胞。
短句来源
  “水貂肠炎”译为未确定词的双语例句
     Production of Monoclonal Antibodies Against Mink En-teritis Parvovirus
     抗水貂肠炎细小病毒单克隆抗体的建立
短句来源
     The virus which had been passaged 4-15 times was used as vaccine. The immunizing dose is 1 mL containing 103.0TCID50 virus. It will give protection for 6 monthes or longer, the protective rate will be more than 95%.
     用FPV弱毒4~15代病毒研制成功的水貂肠炎弱毒疫苗,各项试验证明,该苗安全可靠,免疫剂量为10~(3.0)×1mL,免疫效果好,保护率在95%以上,免疫期6个月以上.
短句来源
  相似匹配句对
     Diagnosis of Mink Enteritis Caused by Coronavirus
     冠状病毒感染引起水貂肠炎的诊断
短句来源
     Pathomorphological Study of Experimental Mink Parvovirus Enteritis
     实验性水貂细小病毒性肠炎的病理形态学研究
短句来源
     Mink Farming Industry in Denmark
     丹麦水貂饲养业
短句来源
     Mink Farming in Denmark
     丹麦的水貂养殖业
短句来源
     Antibiotic Associated Enterocolitis——(29 Cases Analysis)
     抗生素相关性肠炎
短句来源
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  mink enteritis
Methods for preparation of complement fixing antigen from tissues of minks infected with mink enteritis virus have been developed.
      
The relation of mink enteritis virus and feline panleucopenia is discussed.
      
The viruses causing feline panleucopaenia and mink enteritis are shown to have the morphology of parvoviruses, with a mean diameter between 21 and 24 nm.
      
Antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional
      
The antigenic relationships between three similar parvoviruses, canine parvovirus type 2 (CPV), feline panleukopenia virus (FPV) and mink enteritis virus (MEV) were investigated.
      
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This paper reports the etiology of outbreaks of acute infectious mink enteritis occurring in Shandong province and Qinghai province in December 1983, where minks were bred. The average incidence and mortality in 7 breeding farms were 66.48% and 15.98% respectively. Sixty-four specimens were collected from 4 farms.Isolation and identification of virus were made by using secondary feline kidney cells ( FKC ) culture technique .Six viruses were isolated and identified as mink paryo-virus by electromicroscopy, serological...

This paper reports the etiology of outbreaks of acute infectious mink enteritis occurring in Shandong province and Qinghai province in December 1983, where minks were bred. The average incidence and mortality in 7 breeding farms were 66.48% and 15.98% respectively. Sixty-four specimens were collected from 4 farms.Isolation and identification of virus were made by using secondary feline kidney cells ( FKC ) culture technique .Six viruses were isolated and identified as mink paryo-virus by electromicroscopy, serological tests and physicochemical charac-teristics.This is the first mink enteritis virus isolated by cell culture method ia our country.

从山东和青海省的水貂肠炎流行区采集的64份病料中,用幼猫肾次代细胞分离到6株水貂肠炎病毒(MEV)。经电镜、理化学和血清学试验证实为水貂细小病毒(Mink Parvovirus)。

Inactivated CPV antigen, anti-CPV antiserum, glutaraldehyde-fixed pig red blood cell(PRBC), microtitration plates and diluters are contained in a kit and may be used for rapid diagnosis of canine parvovirus infection, feline panleukopenia and mink enteritis. The kit is adaptable for laboratory and field samples and results can read within 4 hours. Reagents in the kit can be stored and used for more than one year without any significant loss in sensitivity.

本文报道,由犬细小病毒灭活抗原、特异抗体、戊二醛醛化猪红细胞及微量血凝板、稀释棒等组成的犬、猫、貂细小病毒通用快诊盒,可用于犬细小病毒性肠炎、猫泛白细胞减少症和水貂肠炎的特异诊断,适合于基层和野外应用,并可在接到病料后4小时内报告结果,有关试剂的有效期在一年以上。

Using the high speed centrifugation, PEG precipitation, sucrose and CsCl density gradient centrifugation, and CsCl equilibrium density centrifugation, the virus particles were purified from the feline kidney cell culture infected mink enteritis virus. The viral structural protein and restriction endonuclease site for the viral RF-DNA of the virus particles were analysed.

应用高速离心—PEG沉淀—蔗糖和氯化铯密度离心—氯化铯平衡密度梯度离心等方法,从水貂肠炎病毒猫肾细胞培养物中提纯病毒粒子。提纯的病毒粒子分为氯化铯浮密度为1.32~1.36g/ml的空壳病毒粒子和氯化铯浮密度为1.40~1.42g/ml的实心病毒粒子。应用SDS—PAGE分析,实心病毒粒子有结构蛋白3种,(VP_1,VP_2、VP_3),空壳病毒粒子有2种(VP_1、VP_2);从第5d培养物中提纯的实心粒子的VP_3含量少于第7d培养物的量。从第7d培养物中提纯的实心病毒粒子经尿素、NP_(40)、TritonX—100裂解后,在薄层聚丙烯酰胺等电聚焦电泳中出现4条蛋白带。从感染48h的细胞培养物中提取到水貂肠炎病毒线状双股复制型DNA(RF—DNA)。通过琼脂糖凝胶电泳测定,该RF—DNA大约有5000个硷基对。经限制性内切酶分析,RF—DNA有2个HindⅢ酶切点,1个PstⅠ酶切点和1个ECoRⅠ酶切点。

 
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