At the same time,ethylene production measurement and storage experiment of tomato fruits were carried out. 【Result】The hybridization results indicated that the expression of fruit ripening-related genes,such as LeACO3 and LeEBF1,and pathogenesis-related protein genes,such as LePR1,LePR5 and LeNP24,were reduced sharply.
The Northern blot hybridization results indicated that the PR-NP24 expressed mainly in fruit and root,and it was inhibited in the wounded leaves of the wild type tomato and the Nr tomato,but its expression in the wounded leaves of the rin tomato had no significant change.
was constructed with SRAP markers using a population consisting of 114 F2 individuals derived from the cross of 4G21(C.melo var.chinensis)and 3A832(C.melo var.saccherinus). Twenty-nine primer pairs were used and produced 187 polymorphic loci. The map consists of 12 linkage groups,which include 152 genetic markers,and covers 2 077.1 cM with an average genetic distance of 13.67 cM.
Shenyan12 is derived from the cross of the male sterile-fertile line AB09 and the sweet pepper self-bred line 99-4-10. Its fruits are green, glossy and elongated bell-shaped at the size of 14.6×8.5×0.4cm, averagely weigh 140g, and contain 958mg kg-1 Vc with a little pungency and over 85% edible rate.
In this experiment , the classical genetic method is adopted to study the law of female segregation of the F_1, F_2, BC_1 , generation of cucumber by the cross of gynocious lines with monoecious lines and andromonoecious lines , and the cross of subgynocious lines with monoecious lines and gynocious lines.
Qingan 78 is a new cabbage F1 hybrid developed by crossing self-incompatible(SI) line BD2549-5-6-8 with SI line YP0326-8-5-2-8.Both lines have high resistance to diseases,good qualities and high combining ability.
Chunsi No. 1 is a new towel gourd F1 hybrid developed by crossing two inbred lines 99-1-10 with 2000-1-1,it has characteristics of high yield, early harvest time, good commercial character and good quality.
When the hybrid seeds were germinated on the 1/2MS medium with 2.5 mg/L 6-BA and 0.5 mg/L NAA,the ratio of seed germination was about 70%. There was no significant difference in the ratio of seed germination between direct crossing and reciprocal crossing.
The karyotype analysis and physical locations of 45S rDNA were carried out by means of fluorescence in situ hybridization in three species, and two forms of Sophora, two species of Robina, and one species of Amorpha.
Also, fluorescence in situ hybridization (FISH) was conducted to detect nuclear derivation of the embryos.
based on rDNA and Cot-1 DNA fluorescence in situ hybridization
Cot-1 DNA contains rDNA and chromosome sites identity between Cot-1 DNA and 25S rDNA was determined by dual-colour fluorescence in situ hybridization.
The spatial expression of CAPON and Dexras1 mRNA was examined by a combination of in situ hybridization (ISH) and immunofluorescence.
Crossing time and renewal numbers of two PH-renewal processes
This paper considers how to find some joint distributions and their marginal distributions of crossing time and renewal numbers related to two PH-renewal processes by constructing an absorbing Markov process.
The NNR is a crossing area of palearctic species and oriental species on the zoogeographical regions, and it is a transitional area from the oriental realm to the palearctic realm.
As for classification methods, this article pays special attention to the syntactic-semantic approach for online Chinese character recognition, as well as the metasynthesis approach for discipline crossing.
The fluctuation of δ18O in precipitation during the monsoon season is very pronounced; the lower values are usually related to farther distance and multilayer moisture transport, as well as moisture crossing the Himalaya Mountains.