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杂交     
相关语句
  hybridization
    IDENTIFICATION OF HBcAG GENE FRAGMENTS BY SOUTHERN BLOT HYBRIDIZATION
    应用Southern吸印滤膜杂交法检测HBcAg基因片段
短句来源
    A STUDY ON NUCLEIC ACID HYBRIDIZATION OF HUMAN NASOPHARYNGEAL CARCINOMA WITH EBV--W FRAGMENT PROBE
    人鼻咽癌DNA与EBV-W片段探针的核酸分子杂交研究
短句来源
    DNA Hybridization in Situ on the Gel and Its Application to the Abnormal Sex Differentiation
    DNA的凝胶原位杂交法在性别分化异常中的应用
短句来源
    APPLICATION OF THE ~(35)S-LABELED PROBE IN NUCLEIC ACID MOLECULAR HYBRIDIZATION
    ~(35)S标记探针在核酸分子杂交上的应用
短句来源
    Nucleic Acid Hybridization Analysis of Regulatory Effect of Cytoplasmic Factor on Malignancy of Myeloma Cells Ⅰ. β-globin Gene Expression
    网织红细胞胞质因子调控肿瘤细胞基因表达的核酸杂交分析 Ⅰ.β-珠蛋白基因的表达
短句来源
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  hybrid
    Use of first generation hybrid mice(F_1) in production of ascitic monoclonal antibodies
    用杂交F_1小鼠制备单克隆抗体腹水的研究
短句来源
    Homomeric and Heteromeric Interactions of Smad3 and Smad4 Identified with Yeast Two hybrid System
    用酵母双杂交系统研究Smad3和Smad4的相互作用
短句来源
    Construction and expression of “bait plasmid” with hepatitis C virus E2 protein in yeast two hybrid system
    丙型肝炎病毒E2蛋白作为酵母双杂交体系“饵”载体的构建及表达
短句来源
    Cloning and Identification of Tec Family Protein Tyrosin Kinase Itk PH Domain and Detection of its Self activation in the Yeast Two hybrid System
    Tec家族蛋白酪氨酸激酶ItkPH结构域的克隆鉴定及其在酵母双杂交系统中自激活作用的检测
短句来源
    Expression of NS2 gene of hepatitis C virus from yeast two hybrid'bait' vector in yeast
    丙型肝炎病毒NS2基因酵母双杂交“饵”载体构建及表达
短句来源
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  hybridisation
    A DNA sequence polymorphism of 24 controls and 35 patientswith NIDDM revealed by digestion of genomic DNA with the endonucleaseXbaI and Southern blot hybridisation with a complementary DNA clone forhuman glucose transpo(?)
    利用XbaⅠ限制性内切酶、墨迹杂交对24例正常人及35例NIDDM患者的白细胞基因组DNAGT基因进行了分析。
短句来源
  hybridization histochemistry
    NF-кBp65 expression was measured with immunohistochemistry, ICAM-1mRNA expression was detected with in situ hybridization histochemistry and leukocyte infiltration was detected with MPO activity.
    用免疫组化法检测NF-кBp65表达、原位杂交法检测ICAM-1mRNA表达,过氧化氢还原法检测肺组织髓过氧化物酶(MPO)活性。 结果:肺缺血再灌注1h后MPO活性显著升高,与缺血1h和再灌注0.5h相比差异明显(均P<0.01)。
短句来源
    DEVELOPMENT OF N4 mRNA NEURONS IN HIPPOCAMPUS AND FRONTAL CORTEX OF THE RAT—— in situ HYBRIDIZATION HISTOCHEMISTRY AND RT-PCR STUDY
    大鼠海马与前皮质N4 mRNA神经元的发育——原位杂交与RT-PCR研究
短句来源
    The present study was focused on the distribution of 5-HT1A receptor in thespinal dorsal horn as well as its roles in the nociceptive information transmissionby using in situ hybridization histochemistry, antisense oligodeoxynucleotide(AS 0) method and behavior test.
    本研究采用原位杂交组织化学技术、反义寡脱氧核苷酸方法及行为药理学方法,对5-HT_(1A)受体在脊髓中的分布及其在伤害性信息传递过程中的作用进行了探讨,研究内容包括以下两方面:1.大鼠脊髓内5-HT_(1A)受体mRNA阳性神经元的分布
短句来源
    In the present study,by using in situ hybridization histochemistry and immunohistochemistry,the distribution of preproenkephalin mRNA ( PPE mRNA)-containing and met-enkephalin (M-ENK) and leu-enkephalin (L-ENK) immunoreactive neurons in the medulla oblongata of the rat were examined in detail.
    应用原位杂交和免疫组织化学方法,对成年大鼠延髓内含前-原脑啡肽(PPE)mRNA和甲硫氨酸-脑啡肽(M-ENK)与亮氨酸-脑啡肽(L-ENK)免疫反应神经元进行观察。
短句来源
    γ-Aminobutyric acid (GABA)is the major inhibitory transmitter in the vertebrate retina. In carp retina,many subclasses of horizontal and amacrine cells are GABAergic. In the present study, we studied the localization of GABAA receptor subunits (α1、α3、α4、α6, β1-3, γ1-2) and GABAC receptor subunit ρ1 mRNA in carp retina using in situ hybridization histochemistry.
    应用原位杂交组织化学技术,利用同位素[ ̄(35)S]-dATP标记的寡核苷酸探针,在鲫鱼视网膜观察了含GABAA受体α1、α3、α4、α6,β1-3,γ1-2及GABAC受体ρ1亚单位mRNA的神经元分布。
短句来源
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      hybridization
    The karyotype analysis and physical locations of 45S rDNA were carried out by means of fluorescence in situ hybridization in three species, and two forms of Sophora, two species of Robina, and one species of Amorpha.
          
    Also, fluorescence in situ hybridization (FISH) was conducted to detect nuclear derivation of the embryos.
          
    based on rDNA and Cot-1 DNA fluorescence in situ hybridization
          
    Cot-1 DNA contains rDNA and chromosome sites identity between Cot-1 DNA and 25S rDNA was determined by dual-colour fluorescence in situ hybridization.
          
    The spatial expression of CAPON and Dexras1 mRNA was examined by a combination of in situ hybridization (ISH) and immunofluorescence.
          
    更多          
      hybrid
    A class of multiderivative hybrid one-step methods of order at least s+1 is constructed with s+1 parameters, where s is the order of derivative.
          
    Furthermore, a class of A-stable 2 parameters hybrid one-step methods of order at least 8 are constructed, which use 4th order derivative.
          
    A hybrid method for solving variational inequality problems
          
    A two-stage semi-hybrid flowshop problem in graphics processing
          
    In this paper, a two-stage semi-hybrid flowshop problem which appears in graphics processing is studied.
          
    更多          
      hybridisation
    Fluorescence in situ hybridisation (FISH) is a rapid and reliable technique for chromosomal investigations that is used for a wide variety of cytogenetic purposes at present.
          
    This review articlepaper presents the general principles of in situ hybridisation, probe labelling and examples of proper use of different kinds of probes.
          
    Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression.
          
    She died 10 years after the onset of the illness and at autopsy the brain showed characteristic changes of progressive multifocal leukoencephalopathy (PML) with the presence of the JC virus confirmed by in situ hybridisation.
          
    By means of restriction endonuclease digests and DNA/hybridisation studies we analysed ten representative methicillin-resistantStaphylococcus aureus strains of our collection for plasmid similarities and plasmid associated resistance determinants.
          
    更多          
      hybridization histochemistry
    Immunohistochemistry andin situ hybridization histochemistry (ISHH) were used to detect the expression of bcl-2 and bax genes.
          
    The sections of kidneys were examined by an electric microscope, PAS staining, immunohistochemical staining and in situ hybridization histochemistry.
          
    The expressions of c-fos/c-jun in cultured rat hippocampus cells (1 d, 3 d, 5 d, 7 d, and 10 d) were studied by using both in situ hybridization histochemistry and SABC immunohistochemistry techniques.
          
    Testicular expression of both porf-1 and -2 was analyzed as a function of maturational stage, aging and hypophysectomy by the solution hybridization/nuclease protection assay, and cellular location determined byin situ hybridization histochemistry.
          
    VP mRNA in the paraventricular and supraoptic nuclei (PVN and SON) and corticotrophin-releasing factor (CRF) mRNA in the PVN were measured by in situ hybridization histochemistry.
          
    更多          


    The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by either...

    The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by either limiting dilution or micromani-pulation methods and a cell line thus obtained was designated as CBH-1.After ten months of culture in vitro up to 69 generations,the ability of this cell line to secret specific monoclonal antibody to PDRBC was not decreased. The CBH-1 cells were also ino-culated into the peritoneal cavity of Balb/cJ or Balb/cCr mice and could be kept in these mice for at least four transplantation generations.The ability of three different agents for inducing the ascitic fluid was compared.As a result,pristane was found to be superior to liquid paraffin and Arlacel A. Attempt was also made to cultureCBH-1 in serum-free medium instead of the conventional medium containing 20% newborn calf serum (NCS).When the serum-free medium was provided with 5γ transferrin per ml,the CBH-1 cells could be maintained just as well as that in DMEM+20% NCS within 15 days (Table 2) The proportion of plaque forming cells after the first cloning was found to be 97.5% and this was increased to 99.8% after second cloning (Table 3).This result implies that the cell popula-tion of the CBH-1 clone was very ho-mogeneous with respect to the secre-tion of the specific antibody to PDR-BC.

    以北京鸭红血球为抗原免疫Balb/cJ小鼠,三天后取脾细胞与小鼠NS-1骨髓瘤细胞在PEG作用下,用两种方法进行细胞融合。只有淋巴细胞杂交瘤细胞能在一种选择性培液——HAT液中生长,具有分泌抗体活性的杂交瘤细胞,借助其上清液的斑点试验加以选择。对斑点试验阳性的杂交瘤细胞进行克隆化和扩大培养,得到能专一分泌抗北京鸭红血球单克隆抗体的淋巴细胞杂交瘤,定名为CBH-1。在体外经过69代培养,历时十月余,分泌能力仍未见衰减。体内可传4—5代,腹水中可检出大量分泌抗体。用降植烷、Arlacel A和石蜡油诱发动物的腹水,其量以降植烷最高、石蜡油次之。使用国产石蜡油作为诱发腹水试验似具有一定实用价值。加5γ转铁蛋白至每毫升不含牛血清的DMEM液中,在15天的培养期内,结果至少与加20%小牛血清的培液的结果相似。首次克隆化后的CBH-1细胞中,空斑形成细胞达97.5%,再次克隆化的细胞中,空斑形成细胞达99.8%,证明是均一的细胞群落。本文还就杂交瘤制备过程及细胞稳定性问题进行了讨论。

    Fragments of EBV-DNA have been inserted into phage M 13mp8 and replicated in E coli K 12JM103, The size of cloned DNA fragments was determined by agarese gel electrophoresis and their viral origin was identified by blot hybridization.The preparation of single-stranded (ss) DNA from recombining phages is the fastest way to obtain large amounts of pure cloned DNA. It provides about 5-10 ug of ss-DNA per ml of culture.

    本文报告EB病毒核酸片段与嗜菌体M_(13)mp8 DNA重组和鉴定方法的研究,来源于细菌质粒的EB病毒核酸片段与嗜菌体DNA重组并在大肠杆菌中繁殖。从嗜菌体中分离单链和双链的核酸,用凝胶电泳和核酸杂交实验分析鉴定重组的核酸。

    The induction of transplantation tolerance was successfully established by the inoculation of allogeneic lymphocytes T depleted or the lymphocytes of F1 origin. The mechanism of the tolerance was studied through adoptive transfer test and GVHR assay in vivo. The results showed that the neonatal induced transplantation tolerance was specific, and its maintenance might be rendered to an active inhibition in which the suppressor T cells were involved.

    本文用去T细胞的同种细胞及杂交一代(F_1)细胞成功地建立了新生小鼠移植耐受模型。通过耐受小鼠脾淋巴结细胞体内过继转移试验及移植物抗宿主反应试验(GVHR),对耐受的机理进行了探讨。实验结果表明,新生期诱导的移植耐受是特异性的,耐受的维持与T抑制细胞(Ts)参与的主动抑制机理有关。

     
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