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杂交
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  hybridization
    Diagnosis of the Gene Deletion D/BMD Proband and Carrier by Fluorescent in Situ Hybridization
    应用荧光原位杂交技术诊断基因缺失型D/BMD先证者和携带者的研究
短句来源
    Detection of HBV DNA in Serum with Spot Hybridization
    应用斑点杂交法测定血清中乙型肝炎病毒的DNA
短句来源
    The technique of HBV-DNA molecular hybridization and its application in clinical medicine
    HBV—DNA分子杂交技术的建立及其临床方面的应用
短句来源
    The detection of HBV DNA in leucocyte by using molecular hybridization technique
    应用分子杂交技术检验白细胞内HBV DNA
短句来源
    SERUM DIRECT SPOT HYBRIDIZATION TEST DETECTION OF HBV-DNA AND THE FACTORS INFLUNC1NG ITS SENSITIVITY AND SPECIFICITY
    血清直接斑点杂交试验检测HBV-DNA及其影响因素的探讨
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  gene hybridization
    1. Technology of fine processing was firstly used to make the solo QOGS and then the gold electrode was etched around two sides of sensor. Different thickness and diameter of electrodes were compared in order to determine its influence to the detection of gene hybridization.
    1.首先利用精细微加工法制作单个石英谐振式传感器,并在其两面光刻出金膜电极,比较了不同电极厚度及直径对基因杂交检测的影响。
短句来源
    Conclusion In the piezoelectric gene sensor arrays, proper probe concentration enhances gene hybridization, but excessive concentration would inhibit gene hybridization, which could probably be explained by the steric and electrostatic hindrances.
    结论 在石英晶体传感器阵列上 ,基因杂交效率随探针浓度增加而增大 ,但探针浓度过高反而抑制杂交 ,其原因可能主要在于探针之间的空间构象关系和静电排斥。
短句来源
    Conclusion Proper ionic strength is beneficial for gene hybridization, but high ionic strength is harmful to the oscillation of piezoelectric crystal.
    结论 一定的盐离子浓度促进基因杂交 ,但盐离子浓度过高则对晶体振荡性能产生负面影响
短句来源
  “杂交”译为未确定词的双语例句
    Studies on Application of Two DNA Probes in Detecting M.TB
    两种DNA探针杂交在检测结核杆菌中的应用研究
短句来源
    DOUBLE STAINING FOR SIMULTANEOUS DETECTION OF HBV DNA AND HBsAg
    HBV DNA原位杂交与HBsAg免疫金银法双标记技术
短句来源
    USING DOT-HYBRIDIZATION TO EXAMINE HBV-DNA IN TRANSFUSIBLE BLOOD
    用斑点核酸杂交检测临床“合格血”HBV-DNA
短句来源
    Detection of Leptospiral DNA in the Serum of Patients with Non-radioactive Labelled Probes
    非放射性探针杂交检测早期钩端螺旋体病患者血清钩体DNA的研究
短句来源
    PREPARATION OF DIGOXIGENIN LABELLED PROBE AND DETECTION OF HEPATITS C VIRUS RNA IN LIVER
    地高辛标记探针原位杂交检测肝组织中HCV RNA
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  hybridization
The karyotype analysis and physical locations of 45S rDNA were carried out by means of fluorescence in situ hybridization in three species, and two forms of Sophora, two species of Robina, and one species of Amorpha.
      
Also, fluorescence in situ hybridization (FISH) was conducted to detect nuclear derivation of the embryos.
      
based on rDNA and Cot-1 DNA fluorescence in situ hybridization
      
Cot-1 DNA contains rDNA and chromosome sites identity between Cot-1 DNA and 25S rDNA was determined by dual-colour fluorescence in situ hybridization.
      
The spatial expression of CAPON and Dexras1 mRNA was examined by a combination of in situ hybridization (ISH) and immunofluorescence.
      
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  gene hybridization
Functional Gene Hybridization Patterns of Terrestrial Ammonia-Oxidizing Bacteria
      
Different patterns were observed among different clusters from both the nod and nif gene hybridization studies.
      
The white arrow indicates the site of the 22-kD zein B gene hybridization on the other chromosome 7 homolog.
      


An ELISA method was established for screening monoclonal antigbodies on the surface of platelets and other cells, and was compared with Radioimmunoassay(RIA). 10ng/100ul level of anti-platelet antibody AN51 gave a positive reaction by ELISA method. In 100 samples of hybridoma supernatant, ELISA and RIA gave 55 and 57 positive results respectively, with no statistical difference (P>0.5). The rate of coincidence was 90%. OD value of ELISA and epm of RIA were eorelated(r=0.89). Compared to RIA, ELISA was safer...

An ELISA method was established for screening monoclonal antigbodies on the surface of platelets and other cells, and was compared with Radioimmunoassay(RIA). 10ng/100ul level of anti-platelet antibody AN51 gave a positive reaction by ELISA method. In 100 samples of hybridoma supernatant, ELISA and RIA gave 55 and 57 positive results respectively, with no statistical difference (P>0.5). The rate of coincidence was 90%. OD value of ELISA and epm of RIA were eorelated(r=0.89). Compared to RIA, ELISA was safer and simpler, and neededs less antigen cells(1/50 of RIA). So, ELISA was a practical method for hybridoma screening test.

本文介绍一种筛选针对血小板及其它细胞表面抗原的单克隆抗体的酶联免疫吸附法(ELISA)。本方法特异性与敏感性较高,10ng/100ul浓度的抗血小板单克隆抗体即呈明显的阳性反应。与放射免疫法(RIA)同时测定100份杂交瘤培养上清,结果ELISA法55份阳性,RIA法57份阳性,符合率为90%,无统计学差异(P=0.75)。ELISA法OD值与RIA法cpm值之间高度相关(r=0.89)。ELISA简单安全,抗原细胞用量为RIA的1/50,是一种实用的方法。

The technique of Clq-ELISA has been established in this laboratory for the detection of antihuman Clq monoclonal antibodies(McAb). This technique, as verified by displacement test, blocking test, and cross titration test, possesses high specificity. It is more sensitive than double immunodiffusion as verified by the tests of 15 specimens of mouse antihuman Clq serum and has better reproducibility. It is concluded that this technique is very useful for the detection of anti-Clq McAb in the supernatant of hybridoma...

The technique of Clq-ELISA has been established in this laboratory for the detection of antihuman Clq monoclonal antibodies(McAb). This technique, as verified by displacement test, blocking test, and cross titration test, possesses high specificity. It is more sensitive than double immunodiffusion as verified by the tests of 15 specimens of mouse antihuman Clq serum and has better reproducibility. It is concluded that this technique is very useful for the detection of anti-Clq McAb in the supernatant of hybridoma culture.

为检测抗人C1q单克隆抗体,我们建立了固相C1q-ELISA。经取代试验、阻断试验及交叉试验证明本法具有特异性;同时用C1q-ELISA及双向免疫扩散试验检测15份鼠抗人C1q血清抗体滴度,表明C1q-ELISA法较双向免疫扩散法高20156倍,用6份血清试验批内及批间的变异系数范围,分别为1.8~6.5%及6.2~16.5%,说明有较好的重现性。因而,本法适用于杂交瘤细胞上清中抗人C1q单克隆抗体的检测。

Epidemiologic data indicate that a large proportion of human population has been exposed to hepatitis B virus (HBV) and infection is of common occurrence. According to the molecular gen-tic theory, hepatitis B infection is due to HBV DNA. So, it is important to establish a convenient laboratory method for the detection of HBV in serum.HBV particles in serum by spot hybridization technique, Dane particles are pelleted by centri-fugation, treated with alkaline, the applied to the nitrocellulose filters. The filters...

Epidemiologic data indicate that a large proportion of human population has been exposed to hepatitis B virus (HBV) and infection is of common occurrence. According to the molecular gen-tic theory, hepatitis B infection is due to HBV DNA. So, it is important to establish a convenient laboratory method for the detection of HBV in serum.HBV particles in serum by spot hybridization technique, Dane particles are pelleted by centri-fugation, treated with alkaline, the applied to the nitrocellulose filters. The filters were neutralized and baked utder vacuum. HBV DNA is detected by its ability to hybridize with 32P-labe-lled DNA prepared by nick translation technique from recombinant NC-1 plasmids which contains the complete HBV genome. After hybridization, the nitrocellulose membrane is washed and autora-diographed on X-ray film. This method is very sensitive and even 0.5pg of HBV DNA can be detected.Eighty-five different titer-positive HBsAg sera were detected by this method. More than 70% were HBV DNA positive. The level of HBV DNA did not quantitatively correspond to the HBsAg level. There were 7 HBV DNA positive in 17 sera with titers of HBsAg less than 1:8. This result impli d that some HBsAg negative sera still can transmitte hepatitis.Twenty-three high titer of HBsAg sera, all being DNA polymerase positive, were examined. All of them were HBV DNA positive.Two sera of DNA polymerase negative were HBV DNA negative. Thus, the endogenous DNA polymerase activity approximately corresponds to the amount of HBV DNA and titer of infectious HBV.

应用斑点杂交法探讨血清中HBV-DNA水平与HBV表面抗原以及内源性DNA多聚酶的关系,结果表明:DNA多聚酶水平可反应血清中DNA水平,HBsAg滴度与HBV-DNA相关,但HBV-DNA的含量与HBsAg滴度并不成正比。

 
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