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杂交
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With restriction endonuclease mapping and blot hybridization methods, arrangements of a-globin genes in 21 patients with hemoglobin H disease in Guangdong Province were determined. Results showed that 10 cases were compounds of nondeletion gene and a-thal 1 gene (ααT/--); 1 case was a homozygote of nondeletion genes (ααT/ααT); 6 cases were compounds of rightward deletion α-thal 2 gene and a-thal 1 gene (-α/--); 4 cases were compounds of leftward deletion a-thal 2 gene and a-thal 1 gene (-α/--).

以限制性内切酶及印迹杂交技术,用α-珠蛋白基因探针分析广东地区21例HbH病病人的α-珠蛋白基因在染色体上的排列,结果10例为非缺失型与α-地贫1的双重杂合子,1例为非缺失型纯合子,6例为右侧缺失型α-地贫2与α-地贫1双重杂合子,4例为左侧缺失型α-地贫2与α-地贫1双重杂合子。

Cell hybridization was done by means of polyethylene glycol (PEG)in suspension.Seven kinds of heterokaryons showing pr- emature condensed chromosomes were obtained.A hybridoma cell line had been established which produced monoclonal anti- body anti human complement 4.On the basis of showing prem- ature condensed chromosomes of heterophasic heterokaryons between CHO metaphase cells and bone marrow lymphocytes from patients with leukemia,their potential proliferation index of bone marrow cell population was studied.Here...

Cell hybridization was done by means of polyethylene glycol (PEG)in suspension.Seven kinds of heterokaryons showing pr- emature condensed chromosomes were obtained.A hybridoma cell line had been established which produced monoclonal anti- body anti human complement 4.On the basis of showing prem- ature condensed chromosomes of heterophasic heterokaryons between CHO metaphase cells and bone marrow lymphocytes from patients with leukemia,their potential proliferation index of bone marrow cell population was studied.Here also were discussed some items,the fusioninducing effects of PEG,the mechanism of the increase of leukemia patients'PPI and the subclioning of hybridoma cell line etc.

本文报告了1980年以来我们在细胞杂交技术及其应用研究中取得的各项阶段性成果。在细胞杂交技术方面,用分子量在1000、2000、4000、6000的PEG为融合介质,获得了中国仓鼠成纤维细胞Wg3-h、人HeLa细胞、人胃腺癌细胞SGC-T901的同种异核体,以及雄性麂肺细胞KIZ-7901-中国仓鼠成纤维细胞Wg3-h、中国仓鼠卵巢细胞CHO-人外周血港巴细胞、中国仓鼠卵巢细胞CHO-正常人骨髓细胞、中国仓鼠卵巢细胞CHO-白血病患者骨髓细胞的异种异核体,并成功地显示了上述各种细胞组合的PCC图象。获得了在体外长期传代培养的小鼠骨髓瘤细胞NS-1-Ag8-BALB/c小鼠脾脏淋巴细胞以及小鼠骨髓瘤细胞SP2/0-Ag14-免疫后的BALB/c小鼠脾脏淋巴细胞的种内杂种细胞系。在细胞杂交技术的应用方面,重点在诱导“熟前凝聚染色体”(PCC),分析白血病患者骨髓细胞的群体增殖潜能。并据以判断白血病患者病情进展、疗效和预后,以及制备分泌抗人C4单克隆抗体的杂交瘤细胞系。本文对PEG的融合性能、白血病患者PPI值升高的机制、杂交瘤细胞的亚克隆化等问题进行了...

本文报告了1980年以来我们在细胞杂交技术及其应用研究中取得的各项阶段性成果。在细胞杂交技术方面,用分子量在1000、2000、4000、6000的PEG为融合介质,获得了中国仓鼠成纤维细胞Wg3-h、人HeLa细胞、人胃腺癌细胞SGC-T901的同种异核体,以及雄性麂肺细胞KIZ-7901-中国仓鼠成纤维细胞Wg3-h、中国仓鼠卵巢细胞CHO-人外周血港巴细胞、中国仓鼠卵巢细胞CHO-正常人骨髓细胞、中国仓鼠卵巢细胞CHO-白血病患者骨髓细胞的异种异核体,并成功地显示了上述各种细胞组合的PCC图象。获得了在体外长期传代培养的小鼠骨髓瘤细胞NS-1-Ag8-BALB/c小鼠脾脏淋巴细胞以及小鼠骨髓瘤细胞SP2/0-Ag14-免疫后的BALB/c小鼠脾脏淋巴细胞的种内杂种细胞系。在细胞杂交技术的应用方面,重点在诱导“熟前凝聚染色体”(PCC),分析白血病患者骨髓细胞的群体增殖潜能。并据以判断白血病患者病情进展、疗效和预后,以及制备分泌抗人C4单克隆抗体的杂交瘤细胞系。本文对PEG的融合性能、白血病患者PPI值升高的机制、杂交瘤细胞的亚克隆化等问题进行了初步探讨。

Utilizing common hemoglobin tests and restriction endonuclease and blot hybridization technology, we analysed hemoglobin composition and α-globin gene organization of five members of a family in Guangxi province. The propositus has normal expression of β-globin gene (βAβA), but is a double heterozygote of α-thal 1 and leftward deletion aaaaaaaaa-thal 2 (-α--). He suffered from HbH disease. Propositus' father and mother are heterozygotes of β-thal (βAβT), but are heterozygote of leftward deletion β-thal 2 (-α/αα)...

Utilizing common hemoglobin tests and restriction endonuclease and blot hybridization technology, we analysed hemoglobin composition and α-globin gene organization of five members of a family in Guangxi province. The propositus has normal expression of β-globin gene (βAβA), but is a double heterozygote of α-thal 1 and leftward deletion aaaaaaaaa-thal 2 (-α--). He suffered from HbH disease. Propositus' father and mother are heterozygotes of β-thal (βAβT), but are heterozygote of leftward deletion β-thal 2 (-α/αα) or heterozygote of α-thal 1 (αα/--). They have no anemic syndrome. The genotype of α-globin gene of two propositus is the same as the propositus' parents but they are heterozygote of β-thal (βAβT) or homozygote of β-thal(βTβT)respectively. No HbH occured in hemoglobin electrophoresis and their anemic syndrome is not so serious as that of the propositus. The results showed that the condition of unequilibrium of α-and β-globin chains and the anemic syndrome were improved in patients with combined α-and β-thalassemias.

利用一般血红蛋白检验,限制性内切酶及印迹杂交技术,分析了广西一个家庭五个成员的血红蛋白组成和α-珠蛋白基因组织,先证者β-珠蛋白基因表达正常(β~Aβ~A),但为α-地贫1与左侧缺失α-地贫2双重杂合子(-α/--),临床上表现为HbH病。先证者父母均为β-地贫杂合子(β~Aβ~T),但又分别为左侧缺失α-地贫2杂合子(-α/--),或α-地贫1杂合子(--/αα),他们无贫血症状。两个弟弟的α-珠蛋白基因型与先证者相同(-α/--),但又分别为β-地贫杂合子(β~Aβ~T)和β-地贫纯合子(β~Tβ~T),二者均无HbH检出,贫血症状也较轻。结果说明α-与β-地贫复合时确使二者不均衡状态改善,贫血症状明显减轻。

 
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