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杂交
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  hybridization
    Comparative Genomic Hybridization Analysis of Human Ovarian Carcinoma Cisplatin-resistan Cell COC1/DDP and Its Parent Cell COC1 and Study on the Mechanisms of Cisplatin Resistance and the Reversal Strategy in COC1/DDP
    COC1与COC1/DDP的比较基因组杂交研究及COC1/DDP耐药机制及逆转的研究
短句来源
    Suppression Subtractive Hybridization Identified Heregulin β1-Responsive Genes Differentially Expressed in Breast Cancer Cells
    采用抑制性差减杂交技术探讨Heregulin β1诱导乳腺癌细胞差异性表达基因的研究
短句来源
    Regulation of Malignant Phenotype and Gene Expression Following Hybridization between Rat Nucleated Erythroblastsand and Mouse Plasmocytoma (SP2/0) Cell Line
    大鼠有核红细胞(中幼、晚幼成红细胞)与小鼠浆细胞瘤(SP2/0)细胞杂交调控恶性表型及基因表达的研究
短句来源
    Application of Fluorescence in Situ Hybridization and Study on Cyclooxygenase-2 in Esophageal Squamous Cell Carcinoma
    荧光原位杂交技术在食管癌研究中的应用及环氧合酶-2与食管癌的相关性研究
短句来源
    Cloning of Genes Related to Differentiation of Leukemia Cell Line K562 by cDNA Subtractive Hybridization
    用cDNA消减杂交法克隆白血病细胞K562分化相关基因
短句来源
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  “杂交”译为未确定词的双语例句
    FESH and Microarray Study on Apoptosis of K562 Cells Induced by BCR-ABL Gene Silence
    应用消减杂交和基因芯片技术研究BCR-ABL基因沉默诱导的K562细胞凋亡
短句来源
    Detection of HGPRT Gene Product before and after Hybridazation of Reticulocytes with Myeloma Cells
    网织红细胞与骨髓瘤缺陷株细胞杂交前后HGPRT酶基因产物的测定
短句来源
    DETECTION OF GENE EXPRESSION IN HEPATOCELLULAR CARCINOMA BY IN SITU
    光敏生物素标记探针原位杂交检测肝癌基因表达
短句来源
    DETECTION OF CHROMOSOME ABERRATIONS IN PERIPHERAL T-CELL LYMPHOMAS BY
    荧光原位杂交技术检测外周T细胞淋巴瘤的染色体畸变
短句来源
    OVEREXPRESSION OF c-myc AND p53 GENE IN HUMAN HEPATO-CELLULAR
    人肝细胞癌c-myc和p53基因的过度表达──免疫组织化学和原位杂交研究
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  hybridization
The karyotype analysis and physical locations of 45S rDNA were carried out by means of fluorescence in situ hybridization in three species, and two forms of Sophora, two species of Robina, and one species of Amorpha.
      
Also, fluorescence in situ hybridization (FISH) was conducted to detect nuclear derivation of the embryos.
      
based on rDNA and Cot-1 DNA fluorescence in situ hybridization
      
Cot-1 DNA contains rDNA and chromosome sites identity between Cot-1 DNA and 25S rDNA was determined by dual-colour fluorescence in situ hybridization.
      
The spatial expression of CAPON and Dexras1 mRNA was examined by a combination of in situ hybridization (ISH) and immunofluorescence.
      
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A mutant cell line (CNf-A) from a human nasopharyngeal carcinoma cell line (CNf) was established and had been maintained in vitro for one year in medium containing 20μg/ml of 8-Azaguanine. The original CNf cell line was derived from a well differentiated nasopharyngeal carcinoma. NO EBNA and viral DNA were detected in this cell line. The CNf-A cell line was selected in medium with gradually increasing amount of 8-Aza- guanine from 0.5-50μg/ml, and it was found to grow well in medium containing 20μg/ml 8-Azaguanine,but...

A mutant cell line (CNf-A) from a human nasopharyngeal carcinoma cell line (CNf) was established and had been maintained in vitro for one year in medium containing 20μg/ml of 8-Azaguanine. The original CNf cell line was derived from a well differentiated nasopharyngeal carcinoma. NO EBNA and viral DNA were detected in this cell line. The CNf-A cell line was selected in medium with gradually increasing amount of 8-Aza- guanine from 0.5-50μg/ml, and it was found to grow well in medium containing 20μg/ml 8-Azaguanine,but not in HAT medium. Hybridized cell lines ( CNf-A/B95-8 ) were obtained by hybridization of CNf-A cell with B95-8 cell and EBNA was detected in these hybridized cell lines.

用8-AG从人的鼻咽癌上皮细胞株CNf建立了一个HGPRT~-自然突变株(CNf-A)。此株细胞对8-AG有抵抗,在20μg/ml 8-AG培养液中维持了一年。HAT培养基能杀死CNf-A细胞。此细胞能与带EB病毒基因的类淋巴母细胞B95-8融合,形成的杂交细胞能在HAT培养液中存活,并可检出EB病毒的核抗原。

The electrophoretic behaviors of two monoclonal hybridoma cells——OBH-1 andOBH-2 and their parent cells——Balb/c mouse spleen cells and NS-1 myeloma cellshave been studied. OBH-1 and OBH-2 are two recently established hybridoma cells which can secret monoclonal antibodies against red blood cells and IgM of BEIJING duck respectively.Balb/c mouse spleen cells displayed two electrophoretio peaks——the one movedat a mobility of 1.3-1.4μ/s/V/cm and the other at a mobility of 0.9-1.0. After spleen cells were treated...

The electrophoretic behaviors of two monoclonal hybridoma cells——OBH-1 andOBH-2 and their parent cells——Balb/c mouse spleen cells and NS-1 myeloma cellshave been studied. OBH-1 and OBH-2 are two recently established hybridoma cells which can secret monoclonal antibodies against red blood cells and IgM of BEIJING duck respectively.Balb/c mouse spleen cells displayed two electrophoretio peaks——the one movedat a mobility of 1.3-1.4μ/s/V/cm and the other at a mobility of 0.9-1.0. After spleen cells were treated with anti-θserum, only the slow peak at a mobility of 0.9-1.0 remained which consisted of chiefly B-cells. The results thus confirmed the claim of other workers that T-cells moved at a faster mobility rate.All the cells of OBH-1, OBH-2 and NS-1 were found to migrate only at a slower mobility rate of about 1.0 and therefore belonged to the cells of B-lymphocyte series.

本文首先探讨了Balb/c小鼠脾细胞及经抗小鼠胸腺细胞血清(抗θ血清)处理的脾细胞的电泳行为,从电泳图谱看出,正常小鼠脾细胞的电泳率呈双峰型,而经抗θ血清处理的仅留下一个慢峰,可见消失的快峰是T淋巴细胞峰。 继之本文探讨了杂交瘤细胞CBH-1、CBH-2及其亲本NS-1细胞的电泳率均属于慢峰,可以证明它们属于B细胞系统的细胞。

Exfoliated epithelial cells from nasopharynx of 27 individuals were collected with a simple negative pressure suction apparatus. These cells were examined cytologically and stained for EBV-nuclear antigen (EBNA)on touch smears with immunoperoxydase(ACIE). DNA was extracted on the remaining cells and studied for the presence of EBV sequences with blot and spot hybridization. Among the 22 positive NPC cases, confirmed by clinical, histological and cytological examinations,EBV-DNA was found with the above-mentioned...

Exfoliated epithelial cells from nasopharynx of 27 individuals were collected with a simple negative pressure suction apparatus. These cells were examined cytologically and stained for EBV-nuclear antigen (EBNA)on touch smears with immunoperoxydase(ACIE). DNA was extracted on the remaining cells and studied for the presence of EBV sequences with blot and spot hybridization. Among the 22 positive NPC cases, confirmed by clinical, histological and cytological examinations,EBV-DNA was found with the above-mentioned two technics in 21 cases, while in 5 cases of chronic inflammation no tumor cell or EBV-DNA was detected.

对27例鼻咽粘膜脱落细胞,用细胞学方法查癌细胞,ACIE法查 EBNA,Blot及打点杂交法查EBV/DNA。经临床及组织学检查确诊的22例 NPC标本EBNA均阳性,其中21例 EBV/DNA阳性;5例炎症标本均未查到癌细胞及EBV/DNA,其中3例因有上皮细胞及淋巴细胞呈现EBNA阳性;ACIE法与两种杂交法所获结果有良好的相关性。

 
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