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酶分离
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  enzyme separation
     Methods PASMCs were obtained by the acute enzyme separation method.
     方法大鼠PASMCs获取采用急性酶分离法。
短句来源
     Its extraction principle, characteristics, distribution influence factors and its application in the enzyme separation are discribed and the problems existing in the current researches and the key themes in the future research are also pointed out.
     文中介绍了该技术的萃取原理、特点、分配影响因素,以及在酶分离过程中的应用,并指出当前研究工作存在的问题和今后研究的重点。
短句来源
     Methods To isolate human mesenteric artery smooth muscle cells with acute enzyme separation method and record the electric current of the calcium-activated potassium channels in these cells using patch clamp single channel technique.
     方法用急性酶分离法分离人肠系膜平滑肌细胞,采用单通道膜片钳技术记录该细胞上的钙激活钾通道电流。
短句来源
     The interaction ways between Tb3+and horse radish peroxidase (HRP) were investigated for the first time using UV-Vis absorption spectroscopy, fluorescence spectroscopy, atomic absorption spectroscopy and enzyme separation technique.
     利用紫外可见吸收光谱、红外光谱、荧光光谱、原子吸收及酶分离方法, 首次研究了稀土离子 Tb3+与植物辣根过氧化物酶(HRP)的相互作用方式.
短句来源
     Application of Aqueous Two-Phase Technology in Enzyme Separation
     双水相技术在酶分离纯化中的运用
短句来源
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  enzymatic isolation
     Enzymatic isolation of leaf cuticles of Garcinia xanthochymus
     大叶藤黄叶片角质层的酶分离技术
短句来源
     Enzymatic Isolation of Human Mesenteric Arteriolar Smooth Muscle Cells and Its Use in Ion Channel Research
     人肠系膜小动脉平滑肌细胞急性酶分离法及其在离子通道研究中的应用
短句来源
     Obj ec tive To establish a method for acute enzymatic isolation of rat tracheal smooth muscle cells (TSMCs) and study the electrophysiological properties of the L-typ e calcium channel of the cells.
     目的建立一种大鼠气管平滑肌细胞急性酶分离法并在分离的细胞上研究其L型钙通道(L-Ca)特性。
短句来源
     Objective To establish an enzymatic isolation method of human mesenteric arteriolar smooth muscle cells and study ion channel characteristics of the isolated cells.
     目的 建立一种人肠系膜小动脉平滑肌细胞急性酶分离法并在分离的细胞上研究其离子通道特性 .
短句来源
  “酶分离”译为未确定词的双语例句
     There were also no significant difference in aggregation rate (92.3% vs. 86.7%, p>0.05) and blastocyst development (58.3% vs. 46.2%; p>0.05 ) between the chimeric embryos derived from 8-cell embryonic blastomeres isolated by 0.25% Pronase and microsurgery.
     采用显微手术法分离黄牛和水牛8-细胞胚胎卵裂球进行聚合,聚合率为92.3%,囊胚发育率为58.3%,与用0.25%链酶蛋白酶分离胚胎卵裂球进行胚胎聚合的聚合率(86.7%)和囊胚发育率(46.2%)均无显著差异(P>0.05)。
短句来源
     Application researches on the rotary disc dynamic membrane filtration technology for
     旋叶动态膜滤技术在酶制剂生产中菌-酶分离过程的应用研究
短句来源
     As the younger one of adult stem cells, dental pulp stem cells (DPSCs) were firstly confirmed by Dr Gronthos in 2000. He defined clonogenic, high proliferative cells isolated by enzyme as DPSCs.
     牙髓干细胞(dental pulp stem cells DPSCs)作为成体干细胞中较年轻的一员,于2000年首次由Gronthos等提出。 他们把通过酶分离具有克隆形成能力的、高增殖率的人牙髓细胞命名为DPSCs。
短句来源
     The expression of LM and FN in generation 1st, 3rd , 6th and 9th of cultured DPC were determined by S-P immunochemical method.
     方法取正常人头皮以两步酶分离法获得毛乳头进行培养,分别对1、3、6、9代毛乳头细胞做LM和FN免疫组化S-P染色,检测其表达情况。
短句来源
     Results: After separation by dispase most of the basal cells from the full skin could be obtained. The colony formation was highest at pH 7.0-7.2 and the medium ionic calcium concentrations of 0.4 mmol/L. Meanwhile,the best serum concentration was 18%,the optimal temperature was 36℃ and the concentration of CO 2 was 6%.
     结果 :表皮细胞以分离酶分离可得到大多数基底细胞 ,在培养液pH值为 7.0~7.2 ,钙离子浓度为 0 .4mmol/L ,血清浓度为 18% ,温度为 36℃ ,CO2 浓度为 6%时 ,表皮细胞克隆形成数最高。
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  enzyme separation
Enzyme separation by starch gel electrophoresis revealed major differences between specimens from the Baltic Sea and those from the North Sea (collected in 1992 and 1993) but a high degree of homogeneity among populations from the same sea.
      
Stability and enzyme separation: Integral representation of the solutions
      
Enzyme separation techniques for the study of growth of cells from layers of bovine dental pulp
      
Effects of different molecular weights and concentrations of polymers on differences of enzyme separation were established.
      
  enzymatic isolation
Methods are described for the enzymatic isolation of endothelial cells from rat and rabbit mesenteric arteries and veins.
      
A technique has been developed for the enzymatic isolation of leaf cells from the Crassulacean acid-metabolism plant Sedum telephium.
      
Since the PM did not disintegrate during enzymatic isolation it appears that lignin also extends into the secondary suberized walls.
      
Intracellular Na+ and Ca2+ in aortic smooth muscle cells after enzymatic isolation in spontaneously hypertensive rats
      
Acinar cells were studied either after enzymatic isolation or in small segments of undisassociated pancreatic tissue.
      
更多          
  enzymatic separation
Enzymatic separation of mesophyll protoplasts and bundle sheath cells from C4 plants
      
The method of enzymatic separation of epidermis from dermis, followed by culture of cells from the dissociated epidermal tissue, provides both epithelial and dendritic cells.
      
This report describes in detail a method of enzymatic separation of adult mammalian muscle using papain.
      
Further studies on ficin may show that it is more suitable for enzymatic separation of tissues generally than the more commonly used trypsin, a major advantage being its use in media containing Ca2+ and Mg2+.
      
Cells were isolated from mouse stomach and spleen by either enzymatic separation or mechanical dissociation.
      
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1. The purity of the alkaline phospbata'e of Amphioxus was raised to 163-fold by the u:e of DEAE-Cellulose (DE-11). An enzyme preparation displaying an electrophoretic homogeneity was obtained.2. Photooxtdation of the alkaline phosphatase led to a rapid loss in activity, the decrease in activity was in accordance with the first order reaction.3. The reaction of the enzyme with N-bromsucciniamide also led to a gradual loss of activity.4. Acety ation of the erzyme or reaction with bromoacetic acid in high concentration...

1. The purity of the alkaline phospbata'e of Amphioxus was raised to 163-fold by the u:e of DEAE-Cellulose (DE-11). An enzyme preparation displaying an electrophoretic homogeneity was obtained.2. Photooxtdation of the alkaline phosphatase led to a rapid loss in activity, the decrease in activity was in accordance with the first order reaction.3. The reaction of the enzyme with N-bromsucciniamide also led to a gradual loss of activity.4. Acety ation of the erzyme or reaction with bromoacetic acid in high concentration resulted in the inhibition, of enzyme activity.The above results seem to show that imidazolyl, indodyl and amino groups are essential for the activity of the alkaline phosphatase.

用DEAE-Cellulose(DE-11)改进了酶的分离提纯方法,将酶提纯163倍,初步获得电泳均一的酶制剂。酶经光氧化后,活力迅速丧失,其失活过程按一级反应进行。酶经N-溴代琥珀酰亚胺作用后活力逐渐丧失。溴代乙酸在较高浓度下亦能抑制酶活力。酶经酰化后活力亦受抑制。实验结果初步表明:咪唑基吲哚基及氨基都是酶活力所必需的基因。

Protoplasts released by EA_3-867 cellulase from tobacco leaves were cultivated in thin layer liquid medium under different conditions for the observation of regeneration of the cell wall.Two methods were used to examine it, namely the staining method (Calcofluor white VBL) and the hypotonic shock method.The results obtained by these methods indicated that a weak light of 600-1000 lux, a density of population of about 10~5 protoplasts/ml and sucrose and mannitol as carbon source are favourable for the regeneration...

Protoplasts released by EA_3-867 cellulase from tobacco leaves were cultivated in thin layer liquid medium under different conditions for the observation of regeneration of the cell wall.Two methods were used to examine it, namely the staining method (Calcofluor white VBL) and the hypotonic shock method.The results obtained by these methods indicated that a weak light of 600-1000 lux, a density of population of about 10~5 protoplasts/ml and sucrose and mannitol as carbon source are favourable for the regeneration of cell wall.

用EA_3-867纤维素酶分离的烟草(Nicotiana tabacum)叶肉原生质体,在不同培养条件下进行液体浅层培养,用荧光增白剂VBL染色荧光法和低渗冲击法研究了再生壁形成的某些培养条件。结果表明,600~1000米烛光的弱光、10~5个原生质体/毫升的密度以及蔗糖或甘露醇作碳源,有利于壁的再生。

The tobacco mesophyll protoplasts are released by EA_3-867 cellulase and cultivated in NT medium (Nagata and Takebe 1971) with thin layer liquid method. Different growth substances are added individually into the basal medium. Afterwards, Calcofluor white VBL is used to examine their effects on the formation of the cell wall. The results are as follows:1. Kinetin is essential for the regeneration of cell wall and 0.25 mg 1~(-1) is adequate suitable (Tab. 1.)2. 2,4-D has no effect on the regeneration of cell...

The tobacco mesophyll protoplasts are released by EA_3-867 cellulase and cultivated in NT medium (Nagata and Takebe 1971) with thin layer liquid method. Different growth substances are added individually into the basal medium. Afterwards, Calcofluor white VBL is used to examine their effects on the formation of the cell wall. The results are as follows:1. Kinetin is essential for the regeneration of cell wall and 0.25 mg 1~(-1) is adequate suitable (Tab. 1.)2. 2,4-D has no effect on the regeneration of cell wall, but its absence decreases the viability of the cell. The optimal concentration of it is about 2 mg 1~(-1) (Fig. 1 A).3. A concentration of coumarin higher than 200 mg 1~(-1) will depress the regeneration of cell wall, and also inhibit the growth of the cell (Fig. 1 B).4. A concentration of triiodobenzoic acid higher than 20 mg 1~(-1) will depress the regeneration of cell wall, and also inhibit the growth of the cell (Fig. 1 C).5. ABA does not inhibit the regeneration of the cell wall, but promotes the budding of the cell (Fig. 1 D, 2, Tab. 3).6. GA_3 at 50 mg 1~(-1) does not effect the regeneration of the cell wall, but promote the growth of the cell; at 100 mg 1~(-1), it will inhibit the regeneration of cell wall and the growth of cell (Tab. 4).The effects of these growth substances on the regeneration of the cell wall are discussed.

用EA_3-867纤维素酶分离的烟草(Nicotiana tabacum)叶肉原生质体,在附加不同生长物质的NT培养基中进行液体浅层培养。用荧光增白剂VBL染色荧光法研究了几种生长物质对再生壁形成的影响。结果表明,激动素为再生壁形成所必需,2,4-D对壁的再生无效,但缺少时会影响细胞的活力;香豆素和三碘苯甲酸分别在200 mg l~(-1)和20 mg l~(-1)以上的浓度下,抑制壁的再生;ABA不抑制壁的再生,但引起出芽;GA_3不影响壁的再生,但100 mg l~(-1)的浓度在一定程度上抑制壁的再生。

 
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