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   循环阈值 的翻译结果: 查询用时:1.015秒
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循环阈值     
相关语句
  cycle threshold
     [Results] The standard curve indicated the linear relationship between CT(cycle threshold) template concentration and showed high correlations (R2= 0.998) .
     [结果]标准曲线循环阈值与模板浓度具有良好的线性关系,R2为0.998。
     The standard curve indicated the linear relationship between CT(cycle threshold) and template concentration with a good correlation (r=0.996) Sensitivity was 5 copies of DHBV genome.
     本实验建立的标准曲线循环阈值(Ct值)与模板浓度具有良好的线性关系,相关系数为0.996; 将其检测极限的Ct值通过标准曲线换算成拷贝数约为 5copies/L,相当于5个DHBV颗粒。
     The results showed that when the concentrations of DNA to be amplified were ranged from 1.725×107 to 1.725×10 10 cps/ml, there was a good correlation between template concentration and cycle threshold, and the correlation coefficient reached to -0.999376. In conclusion, real time FQ-PCR which is specific, sensitive and accurate can be used to further research on human ermap.
     结果显示 ,当待扩增DNA浓度在 1.72 5× 10 7cps ml- 1.72 5× 10 1 0 cps ml范围时 ,模板浓度与循环阈值 (Ct)之间的相关性良好 ,相关系数r2 达到了 - 0 .999376。 结论 :荧光定量PCR方法检测ermap基因 ,具有高敏感性、高特异性和高精确性等优点 ,可作为进一步研究ermap的方法。
短句来源
     Results:The standard curve made by the positive clonal standard indicated a good linear relationship between cycle threshold and template concentration(its correlation coeffecient was 0.970)and could be used to detect quantitatively the original copy numbers of apelin gene.
     结果 :阳性克隆标准品制作的标准曲线可显示循环阈值与模板浓度具有良好的线性关系 (r =0 970 ) ,可用于apelin基因表达的起始拷贝数定量测定。
短句来源
     The copy number of residual DNA and the magnitude of cycle threshold(Ct) showed a linear relationship; the coefficient of correlation r2 approached 0.9982. The quantitative result was accurate and reliable.
     疫苗基因的拷贝数与反应的Ct(循环阈值)值呈良好的线性关系,相关系数r2值达到0.9982,定量结果准确可靠。
短句来源
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  threshold cycle
     A linear relationship between threshold cycle (Ct) of the quantitative real-time PCR and the DNA copy number could be demonstrated (r=0.999).
     结果建立的荧光定量PCR标准曲线的循环阈值(Ct)与模板拷贝数呈良好的线性关系(r=0.999)。
短句来源
     With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061×103 to 3.061×109 cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value.
     结果所建立方法的最低检测限度为15个基因拷贝/反应,在待扩增DNA浓度为3.061×103 cps/ml-3.061×109cps/ml范围时,模板浓度与循环阈值(Ct)之间的相关性良好,决定系数r2为0.988243。
短句来源
     Results For the quantitative real-time PCR, the relationship between the values of threshold cycle (Ct) and the DNA copy number was linear (r= 0.999) and the sensitivity was about 100 times higher than that of the nested PCR for detecting the same DNA sample.
     结果建立的定量标准曲线的循环阈值(Ct)与模板拷贝数呈良好的线性关系(r=0.999); 与巢式PCR相比较,荧光定量PCR检测敏感性是其100倍。
短句来源
     The standard curve was established with the 23S IVS DNA in DNA sequence detection system (ABI 7900HT) and the relationship between the values of threshold cycle (Ct) and the DNA copy number was linear (r=0.997), and the sensitivity of this real-time quantitative PCR was about 100 times higher than that of the nested PCR used to detect homologous DNA.
     结果建立的定量标准曲线的循环阈值(Ct)与模板拷贝数呈良好的线性关系(r=0.997); 与套式PCR相比较,荧光定量PCR检测敏感性是其100倍。
短句来源
     Results The standard curve was established with the standard template and the relationship between the value of threshold cycle(C_(?)
     结果建立的定量标准曲线的循环阈值(C_(?)
短句来源
更多       
  threshold cycle value
     With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061×103 to 3.061×109 cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value.
     结果所建立方法的最低检测限度为15个基因拷贝/反应,在待扩增DNA浓度为3.061×103 cps/ml-3.061×109cps/ml范围时,模板浓度与循环阈值(Ct)之间的相关性良好,决定系数r2为0.988243。
短句来源
  circulated threshold
     RESULTS: ①Five gradient positive templets presented typical S shape after quantitatively enlarged. The relative coefficient of initial templet concentration and circulated threshold reached 0.99969 with good linear relationship.
     结果:①5个梯度阳性模板定量扩增后均呈典型的S形,起始模板浓度与循环阈值相关系数达0.99969,具有良好的线性关系。
短句来源
     ④ The second polymerase chain reaction positive templet amplification fluorescence presented S shape, suggesting that quantitative polymerase chain reaction amplification was successful. The relative coefficient between initial templet concentration and circulated threshold reached more than 0.99, with good linear relationship.
     ④第2轮聚合酶链反应阳性模板扩增荧光呈典型的S形,说明定量聚合酶链反应扩增成功,起始模板浓度与循环阈值相关系数达0.99以上,具有良好的线性关系。
短句来源

 

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  cycle threshold
The genes studied displayed a wide expression range with cycle threshold values between 16 and 37.
      
The LOD was modelled as a function of sample replication level and cycle threshold values.
      
The difference in expression between the treatments for various genes ranged from 1.9 to 8.0 cycle threshold.
      
We found significant impact of RNA integrity on relative expression results, mainly on cycle threshold (Ct) values and a minor effect on PCR efficiency.
      
In Scorpion-PCR, the limit of detection was of 1?pg of total DNA and a high correlation (r?=?0.999) was achieved between target DNA quantity and cycle threshold (Ct).
      
更多          
  threshold cycle
We observed a linear relationship between the threshold cycle (Ct) and the initial DNA concentration.
      
After explaining the basic principles, especially the threshold cycle (Ct?value) as the basic measuring unit in real-time PCR, we introduce three quantitation methods currently in use.
      
However, threshold cycle (CT) values obtained by amplification of DNA from egg numbers between 75 and 1,000 did not differ significantly.
      
Using a comparative threshold cycle (Ct) method and albumin as reference gene, the gene copy number by PMP22 gene duplication or deletion on chromosome 17p11.2-12 was quantified.
      
TheGUS andnptII copy numbers in primary transformants (T0) were calculated by comparing threshold cycle (CT) values of theGUS andnptII genes with those of the internal standard,HMG I/Y.
      
更多          
  cycle threshold)
The genes studied displayed a wide expression range with cycle threshold values between 16 and 37.
      
The LOD was modelled as a function of sample replication level and cycle threshold values.
      
The difference in expression between the treatments for various genes ranged from 1.9 to 8.0 cycle threshold.
      
We found significant impact of RNA integrity on relative expression results, mainly on cycle threshold (Ct) values and a minor effect on PCR efficiency.
      
In Scorpion-PCR, the limit of detection was of 1?pg of total DNA and a high correlation (r?=?0.999) was achieved between target DNA quantity and cycle threshold (Ct).
      
更多          
  threshold cycle value
The HBV DNA levels were calculated by comparing each sample's threshold cycle value to a standard curve obtained in each run.
      
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