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   i型前胶原 在 临床医学 分类中 的翻译结果: 查询用时:0.731秒
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i型前胶原
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  type i procollagen
    Methods Cultured fibroblasts derived from hypertrophic scars (HS) were irradiated with He Ne laser for 30 min at various power densities (10 mW/cm 2, 50 mW/cm 2, 100 mW/cm 2 and 150 mW/cm 2), once a day for 3 consecutive days. In 24 h after repeated irradiation collagen production and type I procollagen mRNA level of fibroblasts were measured with the incorporation of [ 3H] proline and blot hybridization techniques respectively.
    方法 以不同功率密度 (10、5 0、10 0和 15 0mW /cm2 )He Ne激光照射培养增生性瘢痕成纤维细胞 30min ,1/d ,连续照射 3d ,在 3d重复照射后 2 4h用3H 脯氨酸掺入法和斑点杂交法分别检测成纤维细胞胶原合成和I型前胶原基因表达。
短句来源
    Results Collagen synthesis and type I procollagen mRNA level remained unchanged when the laser was irradiated at the power density of 10 mW/cm 2 or 50 mW/cm 2. Compared with control, collagen synthesis and type I procollagen mRNA level were significantly decreased at the power density of 100 mW/cm 2 or 150 mW/cm 2 ( P <0.05).
    结果 培养增生性瘢痕成纤维细胞的胶原合成、I型前胶原基因表达水平在 10、5 0mW /cm2 照射后无变化 ,10 0mW /cm2 重复照射后降低 ,与对照组相比有显著性差异 (P <0 .0 5 ) ;
短句来源
    Type I procollagen mRNA level at the power density of 150 mW/cm 2 was lower than that at 100 mW/cm 2 ( P < 0.05) .
    以 15 0mW /cm2 重复照射 ,细胞胶原合成与I型前胶原基因表达水平亦显著低于对照组 (P <0 .0 5 ) ,且 15 0mW /cm2 照射后的I型前胶原基因表达水平低于 10 0mW /cm2 照射 (P <0 .0 5 )。
短句来源
    Conclusion Repeated He Ne laser irradiation at the power density of 100 mW/cm 2 or 150 mW/cm 2 can suppress collagen synthesis of cultured fibroblasts in HS. The cause of suppression may be associated with down regulation of type I procollagen mRNA expression.
    结论  10 0mW /cm2 或 15 0mW /cm2 功率密度He Ne激光重复照射能抑制培养增生性瘢痕成纤维细胞胶原合成 ,原因可能与I型前胶原基因表达下调有关
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  type i procollagen
Targeted inhibition of type I procollagen synthesis by antisense DNA oligonucleotides
      
Antisense DNA oligonucleotides directed against specific sequences within α1(I) and α2(I) mRNA of type I procollagen were complexed to a cell-specific carrier, and screened for their effectiveness in reducing α1(I) and α2(I) mRNA levels.
      
Osteocalcin, a biochemical marker for osteoblast activity, and the carboxy-terminal propeptide of type I procollagen (PICP), a marker of collagen formation, were slightly but not significantly higher in gastrectomy-treated patients.
      
Simultaneously, circulating carboxyterminal propeptide of type I procollagen (PICP) was released in the perfusion medium (threefold versus C).
      
Gene expression of type I procollagen was significantly greater in Group MH than in Group MC at 7?days postoperatively and was also significantly greater in Group AH than in Group AC at 28?days (P?>amp;lt;?0.05).
      
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Objective To explore the inhibitory effects of He Ne laser repeated irradiation on the collagen synthesis of cultured scar fibroblasts. Methods Cultured fibroblasts derived from hypertrophic scars (HS) were irradiated with He Ne laser for 30 min at various power densities (10 mW/cm 2, 50 mW/cm 2, 100 mW/cm 2 and 150 mW/cm 2), once a day for 3 consecutive days. In 24 h after repeated irradiation collagen production and type I procollagen mRNA level of fibroblasts were measured with the incorporation...

Objective To explore the inhibitory effects of He Ne laser repeated irradiation on the collagen synthesis of cultured scar fibroblasts. Methods Cultured fibroblasts derived from hypertrophic scars (HS) were irradiated with He Ne laser for 30 min at various power densities (10 mW/cm 2, 50 mW/cm 2, 100 mW/cm 2 and 150 mW/cm 2), once a day for 3 consecutive days. In 24 h after repeated irradiation collagen production and type I procollagen mRNA level of fibroblasts were measured with the incorporation of [ 3H] proline and blot hybridization techniques respectively. Results Collagen synthesis and type I procollagen mRNA level remained unchanged when the laser was irradiated at the power density of 10 mW/cm 2 or 50 mW/cm 2. Compared with control, collagen synthesis and type I procollagen mRNA level were significantly decreased at the power density of 100 mW/cm 2 or 150 mW/cm 2 ( P <0.05). Type I procollagen mRNA level at the power density of 150 mW/cm 2 was lower than that at 100 mW/cm 2 ( P < 0.05) . Conclusion Repeated He Ne laser irradiation at the power density of 100 mW/cm 2 or 150 mW/cm 2 can suppress collagen synthesis of cultured fibroblasts in HS. The cause of suppression may be associated with down regulation of type I procollagen mRNA expression.

目的 探讨He Ne激光重复照射对培养增生性瘢痕成纤维细胞胶原合成的抑制作用。方法 以不同功率密度 (10、5 0、10 0和 15 0mW /cm2 )He Ne激光照射培养增生性瘢痕成纤维细胞 30min ,1/d ,连续照射 3d ,在 3d重复照射后 2 4h用3H 脯氨酸掺入法和斑点杂交法分别检测成纤维细胞胶原合成和I型前胶原基因表达。结果 培养增生性瘢痕成纤维细胞的胶原合成、I型前胶原基因表达水平在 10、5 0mW /cm2 照射后无变化 ,10 0mW /cm2 重复照射后降低 ,与对照组相比有显著性差异 (P <0 .0 5 ) ;以 15 0mW /cm2 重复照射 ,细胞胶原合成与I型前胶原基因表达水平亦显著低于对照组 (P <0 .0 5 ) ,且 15 0mW /cm2 照射后的I型前胶原基因表达水平低于 10 0mW /cm2 照射 (P <0 .0 5 )。结论  10 0mW /cm2 或 15 0mW /cm2 功率密度He Ne激光重复照射能抑制培养增生性瘢痕成纤维细胞胶原合成 ,原因可能与I型前胶原基因表达下调有关

Objective In order to explore the selectively inhibitory effects of pulsed Nd:YAG laser irradiation on collagen production of scar fibroblasts in vitro. Methods Cultured fibroblasts derived from hypertrophic scars (HS) and normal human skin were irradiated with a pulsed Nd:YAG laser(wavelength 1 064 nm, pulse width 150(μs) at various energy density levels (500 J/cm 2, 1 000 J/cm 2, 1 500 J/cm 2 and 2 000 J/cm 2). At 24 hours after laser irradiation, collagen production of fibroblasts was...

Objective In order to explore the selectively inhibitory effects of pulsed Nd:YAG laser irradiation on collagen production of scar fibroblasts in vitro. Methods Cultured fibroblasts derived from hypertrophic scars (HS) and normal human skin were irradiated with a pulsed Nd:YAG laser(wavelength 1 064 nm, pulse width 150(μs) at various energy density levels (500 J/cm 2, 1 000 J/cm 2, 1 500 J/cm 2 and 2 000 J/cm 2). At 24 hours after laser irradiation, collagen production of fibroblasts was measured by the incorporation of [ 3H]proline. The expression of proα1(I) procollagen mRNA was investigated by blot hybridization techniques. Results Collagen production of HS fibroblasts was significantly increased, as 2 times as that of normal skin fibroblasts. Type I procollagen mRNA level in HS fibroblasts was markedly elevated, as 3 times as that in normal skin fibroblasts. Collagen synthesis and type I procollagen mRNA level in HS fibroblasts remained unchanged at energy level of 500 J/cm 2, and were significantly decreased at energy density of 1 500 and 2 000 J/cm 2 (P< 0.001 ,P< 0.001 ). Collagen synthesis and type I procollagen mRNA level in normal dermal fibroblasts were markedly decreased at energy density of 1 500 J/cm 2 (P< 0.001 ,P< 0.05 ), and were also reduced at energy denstity of 2 000 J/cm 2 (P< 0.001 ,P< 0.01 ). Collagen production and type I procollagen mRNA level in HS fibroblasts was markedly reduced at energy density of 1 000 J/cm 2 (P< 0.001 ), the dose at which did not affect collagen synthesis and type I procollagen mRNA level in normal dermal fibroblasts. Conclusion Pulsed Nd: YAG laser at energy density of 1 000 J/cm 2 can selectively suppress collagen synthesis and type I procollagen mRNA level of HS fibroblasts.

目的 探讨脉冲掺钕钇铝石榴石 (Nd :YAG)激光对体外培养瘢痕成纤维细胞胶原合成的选择性抑制作用。方法 以不同能量密度 (5 0 0J/cm2 、10 0 0J/cm2 、15 0 0J/cm2 和 2 0 0 0J/cm2 )Nd :YAG激光 (波长 10 64nm ,脉宽 15 0 μs)照射体外培养增生性瘢痕和正常皮肤成纤维细胞 ,照射后 2 4h分别用3 H 脯氨酸掺入法和斑点杂交法检测成纤维细胞胶原合成和I型前胶原基因表达。结果 体外培养增生性瘢痕成纤维细胞胶原合成高于正常皮肤 ,约为正常皮肤的 2倍 ,I型前胶原基因表达亦明显高于正常皮肤 ,约为正常皮肤的 3倍 ;以 5 0 0J/cm2 激光照射 ,增生性瘢痕与正常皮肤成纤维细胞胶原合成、I型前胶原mRNA水平均无变化 ,以 15 0 0J/cm2 、2 0 0 0J/cm2 能量密度激光照射 ,增生性瘢痕成纤维细胞胶原合成、I型前胶原mRNA水平都显著降低 (P <0 .0 0 1,P <0 .0 0 1) ;正常皮肤成纤维细胞胶原合成、I型前胶原mRNA水平在 15 0 0J...

目的 探讨脉冲掺钕钇铝石榴石 (Nd :YAG)激光对体外培养瘢痕成纤维细胞胶原合成的选择性抑制作用。方法 以不同能量密度 (5 0 0J/cm2 、10 0 0J/cm2 、15 0 0J/cm2 和 2 0 0 0J/cm2 )Nd :YAG激光 (波长 10 64nm ,脉宽 15 0 μs)照射体外培养增生性瘢痕和正常皮肤成纤维细胞 ,照射后 2 4h分别用3 H 脯氨酸掺入法和斑点杂交法检测成纤维细胞胶原合成和I型前胶原基因表达。结果 体外培养增生性瘢痕成纤维细胞胶原合成高于正常皮肤 ,约为正常皮肤的 2倍 ,I型前胶原基因表达亦明显高于正常皮肤 ,约为正常皮肤的 3倍 ;以 5 0 0J/cm2 激光照射 ,增生性瘢痕与正常皮肤成纤维细胞胶原合成、I型前胶原mRNA水平均无变化 ,以 15 0 0J/cm2 、2 0 0 0J/cm2 能量密度激光照射 ,增生性瘢痕成纤维细胞胶原合成、I型前胶原mRNA水平都显著降低 (P <0 .0 0 1,P <0 .0 0 1) ;正常皮肤成纤维细胞胶原合成、I型前胶原mRNA水平在 15 0 0J/cm2 激光照射后降低 (P <0 .0 0 1,P <0 .0 1) ,2 0 0 0J/cm2 照射后亦显著降低 (P <0 .0 0 1,P <0 .0 5 ) ;以 10 0 0J/cm2 照射 ,增生性瘢痕成纤维细胞胶原合成与I型前胶原基因表达显著下降 (P <0 .0 0 1) ,而正常皮肤成纤维细胞胶原合成和I型前胶原基因表达却无明显变化。结论  10 0 0J

 
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