Objective To investigate the effect of transforming growth factor-β1 (TGF-β1) on the activity of connective tissue growth factor(CTGF) gene promoter in human renal proximal tubular epithelial cell line HK-2. Methods The regulation fragment of 5' flanking region of human CTGF gene was linked to pGL3-Basic vector.
Methods Human proximal tubular epithelial cell line (HK-2) was cultured in vitro, untreated HK-2 cells were acted as normal control group. HK-2 cells were then stimulated by Ang Ⅱ for 1-24 hours (Ang Ⅱ stimulation group),so that optimal dosage and duration could be chosen.
Methods:Human proximal tubular epithelial cell line HK-2 was pre-treated with AA-I(2.5 mg/L)for 4 hours. Cells were then treated with or without A&A for additional 44 hours. Cell apoptosis was evaluated by using FACS.
To investigate if tumor necrosis factor-alpha (TNF-α) could induce NRK-52E cells, a rat renal proximal tubular epithelial cell line, up-regulating expression of fractalkine, one of chemokines, and if the induced effect appear time- and dose-dependent, and at the same time to investigate if the expression of fractalkine in renal tubular epithelial cells mediate macrophages chemotaxis.
METHODS: Conditioned medium(M-CM) of human peripheral blood MO/MAC was collected and added to HK-2,a human renal proximal tubular cell line. After incubation with M-CM for 24 hours,HK-2 cells were detected for DNA synthesis by -TdR incorporation,osteopontin (OPN) and α-smooth muscle actin (α-SMA) expression by Western blot,and fibronectin(FN) secretion by ELISA.
Objective To observe whether local aldosterone (ALDO) may be synthesized by hum an renal proximal tubular cell lines (HKC) and investigate whether endothelin-1 (ET-1) has effects on the production of local ALDO.
Silencing of Bak Ameliorates Apoptosis of Human Proximal Tubular Epithelial Cells by Escherichia coli-Derived Shiga Toxin 2
Enzymehistochemistry demonstrated that lactate dehydrogenase (LDH) activity in proximal tubular epithelial cells increased but succino dehydrogenase (SDH) activity decreased.
The proximal tubular epithelial cells were cultured in vitro under different conditions.
Our results showed that under different conditions, there were no significant differences in the proliferation of proximal tubular epithelial cells 12 h and 24 h after the culuture (P>amp;gt;0.05).
The proliferation of proximal tubular epithelial cells showed a significant change 96 h after the culture and the cellular proliferation induced by hepatocyte growth factor (HGF) was very active (P>amp;lt;0.05).
The present study was undertaken to determine the role of the ERK activation in H2O2-induced apoptosis of renal epithelial cells using opossum kidney (OK) cells, an established proximal tubular epithelial cell line.
This study was carried out to investigate the effect of aristolochic acid I (AAI) on DNA damage and cell cycle in porcine proximal tubular epithelial cell lines (LLC-PK1 cells).
In the current study, we investigated the oxalate-induced injury and up-regulation of monocyte-chemoattractant protein-1 (MCP-1) in HK-2 cells, a proximal tubular epithelial cell line derived from normal human kidney.
Methods: The mouse lymphocytic leukemia cell line L1210 and pig kidney proximal tubular epithelial cell line LLC-PK1 were used as neoplastic and normal cells, respectively.
This study investigates the potential of an immortalized human proximal tubular epithelial cell line, HK-2, to serve as a representative model for low level exposures of the human kidney to arsenic.
The pig proximal tubule epithelial cells (LLC-PK1 cells) were cryopreserved in hypoxic UW solution (Ar-UW group) or standard UW solution (UW group) at 4°C for 48 h.
We exposed human renal proximal tubule epithelial cells (huRPTECs) to different concentrations of TGF-β1, IL-1α, IL-10, or methionine and measured total Hcy (tHcy) in culture supernatants.
Histopathological examination showed that the necrosis score in the proximal tubule epithelial cells and average apoptitic cell numbers in the CP group were higher than those in the CP+HBO and HBO groups (P>amp;lt;0.05).
There was no statistical difference between the CP+HBO group and the HBO group in terms of necrosis score in the proximal tubule epithelial cells and the percentage of distal tubules containing hyaline casts in the lumen.
Increased functional load on mouse kidney proximal tubule epithelial cells causes changes in nucleolar 3-D architecture