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差示筛选
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  differential screening
     cDNA LIBRARY CONSTRUCTION FROM COMPACTED EIGHT CELL MOUSE EMBRYOS AND DIFFERENTIAL SCREENING FOR SPECIFIC EXPRESSED cDNA CLONES
     小鼠紧密化8-细胞胚cDNA文库的构建及其阶段特异性cDNA克隆的差示筛选
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     Thirty six candidate clones, which were differentially expressed after the treatments,were identified by differential screening.
     通过差示筛选鉴定出了 2 6个PBZ诱导水稻特异表达和增强表达的候选克隆 .
短句来源
     Methods The differential screening of 810 weeks fetal heart cDNA library was performed by cDNA probes synthesized by RNA of white cells of the monozygotical twins. Single pass sequencing of selected cDNA clone was determined. The results were compared with the nucleic acid database in terms of GenBank/EMBL/DDBJ.
     方法 依据该单卵双胎儿童的白细胞RNA合成cDNA探针 ,差示筛选 8~ 1 0周龄胎儿心脏cDNA文库 ,测定各组克隆的序列 ,结果与GenBank/EMBL/DDBJ核酸数据库进行同源性比较 ,所获ESTs进行功能分类 ,并比较患儿与正常儿童表达基因谱的差异。
短句来源
     Conclusion Differential screening of monozygotical twins (one CHD/one normal) is an effective strategy to identify genes associated with CHD.
     结论 一患先天性心脏病另一正常之单卵双胎儿童进行差示筛选 ,是寻找先天性心脏病相关基因和与心脏发育相关基因的有效方法。
短句来源
     On the basis of technology foundation, we divided the RNA technologies into four kinds: Differential Screening(DS), Subtractive Hybridization(SH), RNase Protection Assay(RPA), GeneChip and Microarray based on the molecular hybridization;
     根据RNA分子表达技术的技术基础,将RNA分子技术分为4类:以分子杂交为基础的差示筛选、扣除杂交、RNase保护分析、基因芯片和微阵列;
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  “差示筛选”译为未确定词的双语例句
     2. The differences in gene expression between HepG2X and HepG2Cat cells were then evaluated by suppression subtractive hybridization and PCR.
     ②用抑制差减杂交的方法做HepG2X和对照细胞 (HepG2Cat)的cDNA文库差示筛选
短句来源
     PCR-select cDNA subraction was carried out by reverse transcriptase (RT)-PCR starting with 2 μ g of ployA~+ RNA isolated from the HepG2X and HepG2CAT cell lines.
     ②用Clontech公司试剂盒做HepG2X和HepG2CAT cDNA文库的差示筛选
短句来源
     randomly-chosen 96 recombinant plasmids were screened with cDNA of 'Peiai 64S' and cDNAs of 'Changxuan 3S' before suppression subtraction hybridization and their cDNAs after forward and backward-direction suppression subtractive hybridizations as the template-marking probes and twenty positive candidate clones were chosen;
     采用差减前的‘培矮64S’cDNA和‘长选3S’cDNA以及正向/反向差减杂交后的cDNA为模板标记探针,对随机挑取的96个重组质粒进行差示筛选,获得了20个阳性候选克隆。
短句来源
     Development of Analogy Primer-mediated Differential Display-PCR and Identification of New Genes Differentially Expressed in Human Fetal Liver
     同源引物差示PCR方法的建立及胎肝特异表达基因的差示筛选
短句来源
     Reverse Northern blot was performed to analysis all of the clons in order to exclude false positives further.
     为了进一步剔除假阳性,所有克隆进行了反向Northern差示筛选,挑选杂交信号有明显差异的克隆测序。
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  相似匹配句对
     Screening of Bioflocculant-producing Bacteria
     微生物的筛选
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     Reliability Screening
     可靠性筛选
短句来源
     Methods The differential substraction screening was used for gene cloning.
     方法采用差示杂交法筛选目的基因克隆;
短句来源
     Methods:Differential spectrophtometry.
     方法; 差示分光光度法。
短句来源
     METHODS Differential spectrophtometry.
     方法 :差示分光光度法。
短句来源
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  differential screening
Mitochondrial genes overexpressed in human and monkey B-cell non-Hodgkin lymphomas (B-NHLs) were sought via subtraction hybridization, cloning, and differential screening of the resulting cDNA libraries.
      
Clones Hfb1, Hmob3, and Hmob33 were selected from human brain cDNA libraries by differential screening.
      
Through differential screening, 292 clones in both libraries were identified as being preferentially expressed during fiber development.
      
Based on the cDNA fragment sequence of vernalization-related geneverc203 cloned by differential screening in our lab, the 5' primer has been designed.
      
The differentially expressed cDNA fragments have been obtained by differential screening with cDNA-RAPD technique in photoperiod sensitive genic male sterile (PGMS) rice.
      
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A large and representative cDNA library containing 6.9×10 5 independent clones was constructed from about 2×10 3 mouse embryos at the compacted 8 cell stage to identify and characterize gene products which play crucial roles in the first differentiation process of mouse embryogenesis. The cDNA library was differentially screened with labelled cDNA probes synthesized on poly (A) + RNA isolated from the compacted 8 cell morula or late 2 cell mouse embryos. The results showed that difference in gene expression...

A large and representative cDNA library containing 6.9×10 5 independent clones was constructed from about 2×10 3 mouse embryos at the compacted 8 cell stage to identify and characterize gene products which play crucial roles in the first differentiation process of mouse embryogenesis. The cDNA library was differentially screened with labelled cDNA probes synthesized on poly (A) + RNA isolated from the compacted 8 cell morula or late 2 cell mouse embryos. The results showed that difference in gene expression existed between these two developmental stages. Two clones which appeared only at the 8 cell stage were selected. This study provides a valuable tool for further detailed analysis of specific proteins associated with developmental events.

从小鼠紧密化8-细胞胚构建cDNA文库,采用差示筛选法比较了紧密化8-细胞胚与晚期2-细胞胚在基因表达上的差异。结果显示:两者存在着mRNA序列种类及数量的明显不同,并获得了两个仅存在紧密化8-细胞胚阶段的cDNA克隆。推测其功能可能与植入前胚细胞分化的调控有关。

Objective To evaluate differentially expressed genes between HepG2X and HepG2Cat cells. Methods 1. Recombinant retroviruses encoding the X antigen or bacterial chloramphenicol acetyltransferase were constructed and used to infect HepG2 cells. 2. The differences in gene expression between HepG2X and HepG2Cat cells were then evaluated by suppression subtractive hybridization and PCR. 3. In Situ hybridization and northern blot analysis were carried out to screen these differentially expressed genes. Results...

Objective To evaluate differentially expressed genes between HepG2X and HepG2Cat cells. Methods 1. Recombinant retroviruses encoding the X antigen or bacterial chloramphenicol acetyltransferase were constructed and used to infect HepG2 cells. 2. The differences in gene expression between HepG2X and HepG2Cat cells were then evaluated by suppression subtractive hybridization and PCR. 3. In Situ hybridization and northern blot analysis were carried out to screen these differentially expressed genes. Results PCR select cDNA subtraction generated 8 differentially expressed genes from HBx transfected HepG2 cells (turned on by HBx). All these probes distinguished HepG2X cells from HepG2Cat cells. Two bands (turned off by HBx) cloned from control cells, were detected in HepG2Cat cells but not in HepG2X. One differentially expressed gene C2, the human homology of hu suil, encodes a translation initiation factor whose expression was suppressed by X antigen in HepG2 cells. This gene was also found in most normal liver tissues, not in tumor tissues. Conclusion HBx can regulate the expression of genes whose products may be positive or negative regulators of cell growth. These changes may be in part of the mechanism contributes to hepatocellular transformation.

目的 观察HepG2细胞因乙型肝炎病毒X抗原 (HBx)转染导致的基因表达差异。 方法①用逆转录病毒感染的方法建立表达乙型肝炎病毒X抗原 (HepG2X)及氯霉素乙酰转移酶 (HepG2Cat)的细胞模型。②用抑制差减杂交的方法做HepG2X和对照细胞 (HepG2Cat)的cDNA文库差示筛选。③用3 2 P随机引物标记法标记探针做NorthernBlot杂交及地高辛缺口翻译标记法做原位分子杂交以筛选基因探针。结果 经cDNA文库差减杂交及抑制性PCR扩增共得到 10个cDNA探针 ,其中 8个被HBx所启动 ,2个因HBx而表达下降。经检测 ,这些基因在HepG2X和对照细胞中的表达均有差异。在被HBx所抑制的 2个差示表达基因中 ,1个与人的蛋白翻译起始因子同源 ,同时发现 ,此基因在正常组织中表达较强 ,而在肿瘤组织中表达被抑制。结论 HBx能够改变宿主细胞的基因表达 ,并且这些基因变化可能和肝癌发生有关

A subtracted cDNA library of H.ammodedron (Mey.) Bge seedlings specific to osmotic stress was constructed by suppression subtractive hybridization (SSH), which was composed of approximately 400 individual recombinant clones. SSH was performed between two groups of H.ammodendron (Mey.) Bge. seedlings, the group of seedlings cultivated in Hoagland's (H) solution as driver and the other treated by osmotic stress of mannitol(M) solution as tester. Then 100 recombinant clones from the subtracted cDNA library...

A subtracted cDNA library of H.ammodedron (Mey.) Bge seedlings specific to osmotic stress was constructed by suppression subtractive hybridization (SSH), which was composed of approximately 400 individual recombinant clones. SSH was performed between two groups of H.ammodendron (Mey.) Bge. seedlings, the group of seedlings cultivated in Hoagland's (H) solution as driver and the other treated by osmotic stress of mannitol(M) solution as tester. Then 100 recombinant clones from the subtracted cDNA library were chosen randomly and hybridized with 4 types of probes prepared from unsubtracted M cDNA and H cDNA, forward (M as tester, H as driver) and backward (H as tester, M as driver) subtracted cDNAs respectively. As a result, 21 clones were obtained as the differentially expressed candidates during osmotic stress (Parts of the results are shown as Fig.3). To confirm if they are positive differentially expressed clones, 8 of them were analyzed by Northern blot hybridization (Fig.4). The results showed that the probes built from 3 candidate clones hybridized either only or more strongly with M total RNA (Fig.4 c,d,e), while the other 5 clones had no hybridization signals with H or M total RNA,which probably represented cDNA clones expressed by low abundance transcripts in seedlings under osmotic stress. Sequences of the 3 positive cDNA clones were measured. By BLAST sequence similarity searching in GeneBank nucleotide sequence database, two cDNAs had the same sequence and shared a similarity of 98% to 100% with NaCl specific cDNA clones of Mesembryanthemum crystallinum , the other cDNA had some similarity with the cDNAs encoding senescence associated genes from stored snow pea (Pisum sativum L.var.saccharatum) pods.

以Hoagland溶液培养的梭梭幼苗 (H)为对照群体 ,甘露醇处理的梭梭幼苗 (M )为目标群体 ,进行抑制差减杂交。用经过HcDNA差减的McDNA构建了一个含有大约 4 0 0个独立克隆的差减文库 ;采用差减前的HcDNA和McDNA以及正向 /反向差减杂交后的cDNA为模板标记探针 ,对随机挑取的 10 0个重组质粒进行差示筛选 ,获得了 2 1个阳性候选克隆。从这些阳性候选克隆中随机挑取了 8个进行Northernblot分析 ,证实其中 3个候选克隆代表了在M中特异表达或表达增强的基因 ,序列分析和同源性比较表明它们与逆境胁迫有关 ;而另外 5个候选克隆无Northern杂交信号 ,推测它们为低丰度转录本

 
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