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   尿中核苷 的翻译结果: 查询用时:0.255秒
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尿中核苷
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  urinary nucleosides
     High Performance Liquid Chromatographic Method Development Using Monolithic Column for the Determination of Urinary Nucleosides
     整体柱高效液相色谱法测定尿中核苷的方法研究
短句来源
     HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF URINARY NUCLEOSIDES IN A CANCER PATIENT IRRADIATED ONCE WITH 6 Gy GAMMA RAY
     一例肿瘤病人经6Gy γ线一次照射后尿中核苷的高效液相色谱分析
短句来源
     In this thesis, we studied computational analysis approach to breast cancer metabonomics. Some key problems in analysis of urinary nucleosides and diagnosis of breast have been solved.
     本文在以尿中核苷为肿瘤标记物的乳腺癌诊断研究中引入代谢组学方法,利用代谢组学计算分析方法解决了在核苷分析以及乳腺癌诊断研究中的若干问题,为代谢组学方法用于乳腺癌早期诊断研究作了一些初步的工作。
短句来源
     This study was designed to investigate the usefulness of urinary nucleosides in the diagnosis of gastric carcinoma.
     本研究拟探讨检测尿中核苷在胃癌诊断中的意义。
短句来源
     A method for the determination of urinary nucleosides with reversedphase high performance liquid chromatography is described.
     用反相高效液相色谱法测定尿中核苷
短句来源
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  “尿中核苷”译为未确定词的双语例句
     Two Approaches for Determining the Urinary Excretion Patterns of Nucleosides-HPLC and CE
     尿中核苷排放模式测定的两种方法─-HPLC和CE法(英文)
短句来源
     The significance of the results were discussed in connection with the radiation effects on the intermediary metabolism of nucleic acids especially of DNA, mRNA, and tRNA.
     本文将这些结果联系辐射对核酸,特别是DNA,mRNA及tRNA的中间代谢的影响进行了讨论,并与一例急性放射病事故病人的尿中核苷变化作了比较。
短句来源
     After nucleosides were extracted from urine on phenyl boronic acid affinity chromatography, the analysis was performed on a column (46 mm id×250 mm, 5 μm) at 22 ℃ using a linear gradient elution comprising 25 mmol/L KH2PO4 solution (pH 455) and 60% methanol in water with UV detection at 260 nm.
     通过苯基硼酸亲和色谱法提取尿中核苷,在色谱柱(4 6mmi d ×250mm,5μm)上以25mmol/L磷酸二氢钾溶液(pH4 55)和60%的甲醇水溶液作为流动相进行二元梯度淋洗,于22℃下进行反相色谱分离,260nm处紫外检测。
短句来源
     Meanwhile, HPLC method was also established to analyze the normal and modified nucleosides in human urine. Furthermore, LC/MS/MS method was employed in identifications of fractions from LC separation.
     本文还建立了使用高效液相色谱分析人尿中核苷的方法,并探索了使用LC/MS/MS对液相色谱分离组份进行定性分析的方法。
短句来源
     We developed both reversed-phase high performance liquid chromatographic and capillary electrophoretic approaches to determine normal and modified nucleosides in urine.
     文章给出测定尿中核苷的反相高效液相色谱法和毛细管电泳方法。
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  相似匹配句对
     A method for the determination of urinary nucleosides with reversedphase high performance liquid chromatography is described.
     用反相高效液相色谱法测定尿核苷
短句来源
     Detection of Urinary Modified Nucleosides in Patients with Malignancies
     恶性肿瘤患者尿修饰核苷的检测
短句来源
     Analysis of Urinary Nucleosides by Micellar Electrokinetic Chromatography
     胶束电动力学毛细管色谱法测定尿核苷
短句来源
     Significance of Urinary Modified Nucleosides in Diagnosis for Gastric Cancer
     尿修饰核苷在胃癌诊断的意义
短句来源
     Significance of Urinary Nucleosides in Diagnosis of Gastric Carcinoma
     尿核苷检测在胃癌诊断的意义
短句来源
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  urinary nucleosides
Until now, reversed-phase (RP) HPLC and, to some extent, immunoassays are the preferred analytical methods for urinary nucleosides.
      
Mass spectrometric identification of modified urinary nucleosides used as potential biomedical markers by LC-ITMS coupling
      


The lymphoid region of a patient with Hogkin's disease was irradiated once with 6 Gy gamma ray and the urinary nucleosides were analyzed by high performance liquid chromatography. Ten nucleosides and bases were effectively separated. Five nucleosides (deoxycytidine, deoxyuridine, 7-methylguanosine, inosine, pseudouridine) and one free base (thymine) were shown to increase significantly soon after irradiation, and then gradually to decrease in the following days. The significance of the results were discussed...

The lymphoid region of a patient with Hogkin's disease was irradiated once with 6 Gy gamma ray and the urinary nucleosides were analyzed by high performance liquid chromatography. Ten nucleosides and bases were effectively separated. Five nucleosides (deoxycytidine, deoxyuridine, 7-methylguanosine, inosine, pseudouridine) and one free base (thymine) were shown to increase significantly soon after irradiation, and then gradually to decrease in the following days. The significance of the results were discussed in connection with the radiation effects on the intermediary metabolism of nucleic acids especially of DNA, mRNA, and tRNA.

本文用高效液相色谱法对一例何杰金氏病病人全淋巴区~(60)Coγ线6Gy一次性照射后尿中核苷的变化进行了分析。照后尿中5种核苷(脱氧胞苷、脱氧尿苷、7-甲基鸟苷、肌苷、假尿苷)和一种游离的碱基(胸腺嘧啶)大幅度增加,随后在一周内逐渐减少。本文将这些结果联系辐射对核酸,特别是DNA,mRNA及tRNA的中间代谢的影响进行了讨论,并与一例急性放射病事故病人的尿中核苷变化作了比较。

Cancer patients excrete in their urine increased amounts of modified purines and pyrimidines. These modified mucleosides are primary constitutents of RNA and are synthesized at the macromolecular level. When RNA is catabolized, most of these modified nucleosides cannot be reutilized ; consequently, they are excreted. Due to their increased urinary excretion in conjunction with altered RNA turnover in carcinogenesis, they have been proPO8ed as tumour markers. We developed both reversed-phase high performance...

Cancer patients excrete in their urine increased amounts of modified purines and pyrimidines. These modified mucleosides are primary constitutents of RNA and are synthesized at the macromolecular level. When RNA is catabolized, most of these modified nucleosides cannot be reutilized ; consequently, they are excreted. Due to their increased urinary excretion in conjunction with altered RNA turnover in carcinogenesis, they have been proPO8ed as tumour markers. We developed both reversed-phase high performance liquid chromatographic and capillary electrophoretic approaches to determine normal and modified nucleosides in urine. Data obtained with CE are in good accordance with those from HPLC. The reference excretion levels of urinary nucleosides from healthy volunteers were established, and elevated ones of modified nucleosides from 34 patients with different kinds of cancer were observed. The developed methods are suitable for the analysis of large series of samples for clinical studies.

癌症病人在尿中排放比常人高得多的叶啉和嘧啶,这些改性核耷是RNA的主要组成。由于其增加的排放与癌变的RNA周转有关,已被建议和研究作肿瘤标记物。文章给出测定尿中核苷的反相高效液相色谱法和毛细管电泳方法。两种方法所得数据一致。用此方法建立了正常人尿中核苷的排放水平,并测定了34个癌症病人尿中核苷浓度,观察到改性核着浓度明显的增高现象。结果袭明,两个方法均适于大量尿样的核苷与癌症关系的临床研究。

A micellar electrokinetic chromatography method for identification and quantitation of nucleosides in urine has been optimized. After nucleosides were extracted from human urine on phenyl boronic acid affinity chromatography column, the analysis was performed in an uncoated 52 cm×50 μm i.d. capillary tube using a 300 mmol/L SDS 25 mmol/L borate 50 mmol/L phosphate buffer (pH=6 96), 0 5 Psi×15 s injection, 7.0 kV voltage and UV detection at 254 nm. By using the MEKC method developed, 14 normal and modified...

A micellar electrokinetic chromatography method for identification and quantitation of nucleosides in urine has been optimized. After nucleosides were extracted from human urine on phenyl boronic acid affinity chromatography column, the analysis was performed in an uncoated 52 cm×50 μm i.d. capillary tube using a 300 mmol/L SDS 25 mmol/L borate 50 mmol/L phosphate buffer (pH=6 96), 0 5 Psi×15 s injection, 7.0 kV voltage and UV detection at 254 nm. By using the MEKC method developed, 14 normal and modified nucleosides in 28 normal urines were quantified to establish the reference ranges. The result shows that this MEKC method is reliable, sensitive, efficient and suitable for clinical application.

用胶束电动力学毛细管色谱法测定尿中核苷 .通过苯基硼酸亲和色谱柱对尿中核苷进行纯化 ,以十二烷基磺酸钠 ( SDS) -十水合四硼酸钠 -磷酸二氢钠为缓冲液 ( p H=6.96) ,在未涂层石英毛细管上 ,于 7k V恒压 ,2 9℃下进行电泳分离 ,检测波长为 2 54nm.此方法用于 2 8个正常人尿中 1 4种核苷的测定 ,确定了正常成年人 (汉族 )尿中核苷的排放范围 .

 
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