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减法杂交
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  subtractive hybridization
     COMPARISON OF REPRESENTATIONAL DIFFERENCE ANALYSIS OF cDNA AND SUPPRESSION SUBTRACTIVE HYBRIDIZATION TECHNOLOGY
     cDNA代表性差异分析和抑制性减法杂交技术的比较
短句来源
     Methods Several convenient techniques of molecular biology including subtractive hybridization, suppression PCR, T/A cloning and sequencing, were used to identify genes expressed differentially in CD45 + and CD45 - cells isolated from U266 cells line of multiple myeloma.
     方法 从骨髓瘤细胞株U2 6 6中分离出CD4 5 +和CD4 5 -细胞 ,结合应用减法杂交、抑制性PCR、T/A克隆、测序等技术 ,寻找CD4 5 +和CD4 5 -细胞间的差异表达基因。
短句来源
     A novel tapetum_specific cDNA clone of rice, its corresponding gene designed as RA39, is isolated by RNA subtractive hybridization, differential screening and rapid amplification of cDNA ends.
     利用cDNA减法杂交、差异杂交筛选和RACE等技术 ,从水稻 (OryzasativaL .ssp .japonica)中克隆了一个新的绒毡层特异性cDNA ,其编码基因被命名为RA39。
短句来源
     After the development of about twenty years,some methods for cloning plant genes were established,which included functional cloning,PCR amplifing cloning,transposon or T DNA tagging,postional cloning,differential hybridization and subtractive hybridization,mRNA differential display and artificial synthesis DNA cloning.
     经过近 2 0年的发展 ,已经形成了一些克隆植物基因的方法 ,主要有功能克隆 ,PCR扩增 ,转座子或T DNA标签 ,定位克隆 ,差别杂交和减法杂交 ,mRNA差异显示和人工合成克隆。
短句来源
     In our previous study, a series of new gene fragments was obtained by usingPCR-based subtractive hybridization technique to screen cell differentiation andapoptosis-related genes from retinoic acid-treated promyelocytic cell line HL-60cells.
     利用“基于PCR的减法杂交技术”研究了维甲酸诱导早幼粒白血病细胞分化和凋亡时所表达的特异基因,得到了一系列新基因克隆片段。
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  “减法杂交”译为未确定词的双语例句
     Using modified virtual subtraction method, a poly(A) binding protein gene, DcPAB , had been isolated from carrot somatic embryo.
     运用改进的减法杂交技术分离到胡萝卜Poly(A)结合蛋白基因DcPAB .
短句来源
     One cDNA whose corresponding mRNA was preferentially accumulated in rice florets was isolated by PCR-mediated RNA subtraction hybridization and RACE strategy. This gene, termed RA68 encoded a protein with proline- and threonine- rich motif.
     第一部分利用减法杂交和RACEs从水稻中克隆了一个编码含有脯氨酸和苏氨酸丰富结构域多肽的cDNA,其相应的基因被命名为RA68。
短句来源
     Cloning technique of differentially expressed gene in quantity mainly is mRNA differential display, which is presently one of the most effective methods. However , mRNA differential display possesses higher unreal positive rate,in order to overcome its shortcoming, some novel strategies and methods were advocated ,such as differential subtraction display, subtraction based on LD PCR, LD PCR based on subtraction,those techniques have dominant advantages to mRNA differential display.
     表达量差异的基因克隆技术主要有mRNA差异展示 ,此技术是目前筛选差异表达基因最有效的方法之一 ,但主要存在假阳性率高的不足 ,针对此缺点 ,近几年提出了新的策略与方法 ,如差异消减展示、基于PCR和减法杂交基础上的差异表达基因克隆技术 ,这些技术具有显著优势 .
短句来源
     The subtracted cDNA of newborn larvae (NBL) was prepared using cDNA of NBL as Tester and cDNA of muscle larvae (ML)+adult worm (Ad) as Driver, and was ligated to pT adv vector for the establishment of NBL subtractive cDNA library.
     利用差减杂交 (减法杂交 ,subtractive hybridization)技术 ,以旋毛虫新生幼虫 ( newborn larvae,NBL)的 c DNA作为试验方 ( tester) ,以肌幼虫 +成虫的 c DNA作为驱动方 ( driver) ,制备新生幼虫差减 c DNA; 利用 T载体构建 NBL差减 c DNA文库 ,获克隆株 90个。
短句来源
     Methods Forward and backward cDNA probes were synthesized and directly labeled with alkaline phosphatase,while cDNA fragment library was constructed using suppression PCR.
     方法在用抑制性PCR构建cDNA片段文库的同时,分别制备正、反向减法杂交探针并用碱性磷酸酶直接标记。
短句来源
  相似匹配句对
     Suppression Subtractive Hybridization Technique and its Applications
     抑制性减法杂交技术及其应用
短句来源
     e-Hybrid Rice
     e杂交
短句来源
     northern hybridization.
     Northern杂交
短句来源
     COMPARISON OF REPRESENTATIONAL DIFFERENCE ANALYSIS OF cDNA AND SUPPRESSION SUBTRACTIVE HYBRIDIZATION TECHNOLOGY
     cDNA代表性差异分析和抑制性减法杂交技术的比较
短句来源
     On "Subtracting" Design
     论“减法”设计
短句来源
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  subtractive hybridization
Interspecies Subtractive Hybridization of cDNA from Human and Chimpanzee Brains
      
A New Planarian Extrachromosomal Virus-like Element Revealed by Subtractive Hybridization
      
A combination of suppression subtractive hybridization and a new technique of mirror orientation selection was used to compare the total DNA for two, sexual and asexual, races of freshwater planarian Girardia tigrina.
      
In this study, we adopted the suppression subtractive hybridization (SSH) technique to isolate differentially expressed ESTs from Gossypium barbadense variety 7124 during the Verticillium wilt defense process.
      
Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development sta
      
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An improved method was utilized for constructing hybrid arrested λ phage cDNA library by subtractive hybridization, in which magnetic particles were applied to separate monomers from hybrids, and candidate sscDNAs were enriched by subtracting two control mRNAs from one treated sscDNA. The results showed that this new approach had a efficient improvement in constructing cDNA library which increased the yield of monomers, simplified experimental procedures and enriched more candidate genes.

介绍了一种改进的利用减法杂交构建λ噬菌体cDNA文库的方法,该方法利用处理的scDNA减去两种对照的mRNA富集目的sscDNA并用磁珠法分离杂交单体,结果表明其增加了杂交单体的产量、简化了实验步骤,而且更有利于富集所需基因,是构建cDNA文库的一个有效改进.

Dendritic cells is potent professional antigen presenting cells. It is very helpful in understanding the biological functions of dendritic cells by cloning genes specifically expressed in antigen processing and presenting progress. For this goal, a new strategy based on “long distance” PCR and subtractive hybridization has been designed. By this design the differentially expressed genes of dendritic cells and antigen stimulating dendritic cells were analyzed. As results, the subtracted cDNA were concentrated...

Dendritic cells is potent professional antigen presenting cells. It is very helpful in understanding the biological functions of dendritic cells by cloning genes specifically expressed in antigen processing and presenting progress. For this goal, a new strategy based on “long distance” PCR and subtractive hybridization has been designed. By this design the differentially expressed genes of dendritic cells and antigen stimulating dendritic cells were analyzed. As results, the subtracted cDNA were concentrated on the range of 0 7 kb-6 kb. Sequencing 200 clones inserted with fragments of 0 7 kb-2 kb, 50% clones was new genes, 30% contained code region and 15% contained complete code region. By dot bloting, 80% new genes were confirmed to be the special expression genes in antigen stimulated cell. Also, there are some functional genes have been cloned, and more works is undergoing. The results suggested that it is practical for large scale cloning new genes by this strategy, and this study will be helpful in further understanding the function of dendritic cell.

树突状细胞在抗原提呈过程中起极其重要的作用.为大规模筛选抗原刺激后人树突状细胞特异表达基因,建立了一种基于“长距离” P C R 技术的减法杂交技术,在实验中取得了较好的效果.初步测序分析了 200 个插入片段为 07~2 kb 的克隆,结果发现新基因片段占 50% ,其中 30% 的片段包含基因编码区,15% 的片段包含完整编码区,打点杂交分析新基因中 80% 为抗原刺激后树突状细胞所表达.从已知和未知基因中发现了一些与树突状细胞生物学功能可能相关的基因,这将有助于进一步揭示与阐明树突状细胞的生物学功能.更多及更长片段的测序工作正在进行中.

Vernalization is an essential factor in the flowering development of cold-required plants. There is akey stage of nucleic acid metabolism in the process of vernalization in winter wheat. To probe into the molecular detendnants of vernalization, a cDNA library presumably enriching vernalization-related genes was prepared by specially expressed mRNAs from winter wheat (Triticum aestivum L. cv. Jingdong No. 1 ) plumules at thekey stage of 21 d vernalization being recovered as cDNAs after subtraction with mRNAs...

Vernalization is an essential factor in the flowering development of cold-required plants. There is akey stage of nucleic acid metabolism in the process of vernalization in winter wheat. To probe into the molecular detendnants of vernalization, a cDNA library presumably enriching vernalization-related genes was prepared by specially expressed mRNAs from winter wheat (Triticum aestivum L. cv. Jingdong No. 1 ) plumules at thekey stage of 21 d vernalization being recovered as cDNAs after subtraction with mRNAs from nonvernalized anddevernalized plumules. One vernalization-related cDNA clone (Vrc ), Vrc79, which was only expressed at thekey stage of 21 d vernalization, but not at other stages of nonvernalization, 4 d vernalization and devernalization, was isolated by differential screening of the library, and shown to be a vernalization-specific clone byNorthem blot. Result of homology search suggested that Vrc79 was a novel gene identified in higher plantwhich was different from the cold-stress-induced genes and might play an important role in the floral induction in vemalization-requiring plants.

在低温需求型植物的发育过程中,春化作用是诱导植物成花的必要因子。在冬小麦春化进程中存在着一个核酸代谢的关键期,为了探讨春化作用的分子机理,以不春化及脱春化的冬小麦京冬1号(Triticum aestivumL.cv.Jindong No.1)胚芽的mRNA为对照,通过减法杂交构建了富集冬小麦春化相关基因的cDNA文库,经过差异筛选分离到了一个仅在春化21d这一关键期表达,而在不春化、春化4d、脱春化不表达的春化相关。cDNA克隆Vrc79。Northem杂交及同源性分析表明它是一个在植物中新发现的不同于低温胁迫诱导基因的春化特异克隆,其对春化需求型植物的成花诱导可能起重要的调控作用。

 
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