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   酶活检测 的翻译结果: 查询用时:0.657秒
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酶活检测
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  measurement of the enzyme activity
     The measurement of the enzyme activity show that the expression production can be secreted to the medium, and the enzyme activity was approached the highest level (0 046U/ml) when the culture time was 60h.
     酶活检测显示该基因能在酿酒酵母中表达并分泌到胞外。 发酵液中的酶活在培养 6 0小时达到最高 0 0 4 6U/ml。
短句来源
  “酶活检测”译为未确定词的双语例句
     Standardization of Administration Procedure for Mice Exposed to Cigarette Smoke and the CYP1A1 Gene Expression Detected by an Enzyme Activity
     小鼠吸入香烟烟雾染毒过程的标准化和使用酶活检测在烟熏小鼠中基因CYP1A1的表达(英文)
短句来源
     cancphora was 15.93 (U/ml) at 48 h and up to 18.18 (U/ml) at 108 h.
     刀。 卜D酶活检测在 108 11时,其活性可高达 48.22(U/1ill),而 IO-GOIL则为 18.18(U/:11I)。
短句来源
     And disease resistance of B99267-1 kept stable in all generations which was consistent with enzyme activity detection.
     而B99267-1抗病性较稳定,随着世代的增加抗病性无明显变化,与酶活检测结果一致。
短句来源
     Then, two tobacco plastid expression vectors and two lettuce plastid expression vectors were constructed. They contained the homologous fragments of the plastid genomes respectively, the foreign gene (GUS or NK) expression cassette controled by T7 promoter and the expression cassette of the marker gene of BADH.
     其次,构建了两个烟草叶绿体表达载体和两个莴苣叶绿体表达载体,它们分别包括烟草和莴苣的叶绿体同源片段、不同外源基因(GUS和NK)的含T7启动子的表达盒以及筛选标记基因BADH的叶绿体表达盒,并对所构建载体中的BADH表达盒在大肠杆菌中的表达进行了酶活检测
短句来源
     Objective: To express the full-length encoding sequence of human polypeptide: N-Acetylgalactosaminyltransferase2 in Escherichia coli using vector pGEX-5X-3,and analyze its enzyme activity after purification and renaturation.
     目的:以人类多肽:N-乙酰氨基半乳糖转移酶2(ppGalNAc-T2)为研究对象,利用载体pGEX-5X-3在大肠杆菌(E.Coli)BL21中原核表达其编码序列,并对表达产物进行纯化、复性及酶活检测
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  相似匹配句对
     An Improved Spectrophotometric Procedure for the Laccase Assay
     一种改进的漆检测方法
短句来源
     mobilis CP4 increased gradually from later-logarithm stage to stablestage, and the talB promoter from E.
     mobilis CP4没有检测到转醛醇
短句来源
     examined blood Enzyme method applied for examing blood lipid;
     检测血脂;
短句来源
     APPROACH OF SODIUM AZIDE AFFECTING ENZYME ACTIVITY IN ELISA
     叠氮钠(NaN_3)对ELISA检测影响的探讨
短句来源
     THE TEL OMERASE ACTIVITY IN HUMAN ESOPHAGEAL CARCINOMA
     食管癌端粒性的检测
短句来源
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  enzyme activity assay
Characterization of this protein by enzyme activity assay showed adenylate kinase activity.
      
The result of the enzyme activity assay and the oxidation of glycolate to glyoxylate catalyzed by the whole cells of E.
      
The structural features of the predicted polypeptides as well as an in vitro enzyme activity assay with bacterially expressed recombinant proteins indicated that MdCAS1 and MdCAS2 may indeed function as β-CAS isozymes in apple fruits.
      
The SS activity of each OsSSII was confirmed by expression and enzyme activity assay in Escherichia coli.
      
Comparison of CLPP and enzyme activity assay for functional characterization of bacterial soil communities
      
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  measurement of the enzyme activity
An assay is described that allows the direct measurement of the enzyme activity catalyzing the transfer of the methyl group from N5-methyltetrahydromethanopterin (CH3-H4MPT) to coenzyme M (H-S-CoM) in methanogenic archaebacteria.
      
Immunodetection and measurement of the enzyme activity show that wheat pol CI remains at a constant level during germination, whereas dramatic changes of the replicative DNA polymerase A and B activities were previously reported.
      
Definitive diagnosis is made by measurement of the enzyme activity in erythrocytes.
      
For quantitative measurement of the enzyme activity, duplicate values were determined routinely.
      


Dihydro dipicolinate synthase (DHDPS)is a first step on the lysine biosynthetic path-way. The plamids of the wild E. coli and Triticum aestivum containing DHDPS gene aresuccessfully expressed in RDA8 and RDA8/pDB26. The programs of purification to bothDHDPS from RDAS and from RDA8/pDB26 are different in few steps. Screening for crys-talline conditions is separatedly considered duing to the obviously thermal stability. The suit-able media for crystallization to both wild DHDPS and wheat DHDPS are in differences...

Dihydro dipicolinate synthase (DHDPS)is a first step on the lysine biosynthetic path-way. The plamids of the wild E. coli and Triticum aestivum containing DHDPS gene aresuccessfully expressed in RDA8 and RDA8/pDB26. The programs of purification to bothDHDPS from RDAS and from RDA8/pDB26 are different in few steps. Screening for crys-talline conditions is separatedly considered duing to the obviously thermal stability. The suit-able media for crystallization to both wild DHDPS and wheat DHDPS are in differences withPH, buffer media and salt concentration. We suggest that these differences hint some structurally characteristic behaviors,

DHDPS是赖氢酸合成通路中第一步的合成酶。将中国春小麦的DHDPS基因质粒移植入RDA8中,得到RDA8/pDB26菌株,本研究通过细胞培养、层析提纯和结晶条件的探索,给出了一个较好的技术路线,并为开展义衍射分析蛋白结构创造了条件,通过该研究,对中国春小麦DHDPS和野种大肠杆菌的DHDPS差异有了一定的了解,并且对细胞培养的MM介质处理,DEAE-Sepharose,Phenyl-Sepharose和MonoQ层析的方法给出具体实验条仲。酶活的检测和蛋白浓度测定都是采取高灵敏度的方法。结晶的SCreening条件对于二种DHDPS有很大的差异。对野种大肠杆茵的DHDPS,需要表面活性剂N-octyl-D-glucopyranoside,pH10.0~10.5,对于中国春小麦一DHDPS则未找到较好的表面活性剂,pH6.8~7.6。中国春小麦一DHDPS晶体培养条件在以往文献中未见报导。

The T-cyt gene Coding the key enzyme of cytokinin biosynthesis was placed under control of CaMV 35S,rbc S and T-cyt native promoters respectively.The chimeric plasmids were transferred separatedly into Nicotiana tubacum. The transgenic plants were confirmed by PCR DNA amplification,Southern hybridization and NPT Ⅱ detection.The results of Northern analysis showed that T-cyt gene could express in leaves,stems and roots of tobacco with transgene of caMV 35S/T-cyt. The transcript level of rbc S/T-cyt gene activities...

The T-cyt gene Coding the key enzyme of cytokinin biosynthesis was placed under control of CaMV 35S,rbc S and T-cyt native promoters respectively.The chimeric plasmids were transferred separatedly into Nicotiana tubacum. The transgenic plants were confirmed by PCR DNA amplification,Southern hybridization and NPT Ⅱ detection.The results of Northern analysis showed that T-cyt gene could express in leaves,stems and roots of tobacco with transgene of caMV 35S/T-cyt. The transcript level of rbc S/T-cyt gene activities in leaves was higher than that of stems and little transcript activity was found in roots of those plants. ELISA indicated that the roots of transgenic tobacco contained more isopenteryl transferase. Moreover, a series of morphological and physiological changal, such as promotion of axillary bud growth, inhibition of root srowth, promotion of chlorophyll synthesis and delay of leaf senescence,were observed.

将编码细胞分裂素合成关键酶──异戊烯基转移酶的T-cyt基因分别置于CaMV35S启动子,rbcS启动子和T-cyt基因自身启动子的调控下,构建成不同的嵌合质粒用于转化烟草,通过PCR检测,Southern杂交和NPTⅡ的酶活检测对获得的转基因烟草进行了鉴定.Northern分析表明:CaMV35S启动子驱动下的T-cyt基因在转基因烟草的根、茎、叶中均有mRNA的积累;rbcS启动子指导下T-cyt基因在叶中转录较强,茎中次之,在根中几乎未表达.以根据抗原决定簇进行人工合成的复合抗原免疫家兔得到异戊烯基转移酶抗体,ELISA结果表明转基因烟草根中异戊烯基转移酶含量较高.T-cyt基因的表达对转基因烟草的生长发育有明显影响:其叶绿素a、b含量明显增加,叶衰老迟缓;顶端优势受到抑制,侧芽生长旺盛;与对照相比,其根系不发达.

αAcetolactate decarboxylase(αALDC)gene has been cloned from Baillus brevis using PCR amplification. The amplified 0.97 kb DNA fragment was confirmed to be known αALDC gene by DNA sequencing. The fragment was inserted into the vector pBV220 to construct an expression plasmid pBVYI. This recombinant plasmid over expressed αALDC in E.coli DH5α. The αALDC activity of recombinant bacterium was 10 000fold higher than that of Bacillus brevis. After purification, the properties of the recombinant αALDC...

αAcetolactate decarboxylase(αALDC)gene has been cloned from Baillus brevis using PCR amplification. The amplified 0.97 kb DNA fragment was confirmed to be known αALDC gene by DNA sequencing. The fragment was inserted into the vector pBV220 to construct an expression plasmid pBVYI. This recombinant plasmid over expressed αALDC in E.coli DH5α. The αALDC activity of recombinant bacterium was 10 000fold higher than that of Bacillus brevis. After purification, the properties of the recombinant αALDC were studied. The activity of this enzyme could be stimulated by Mn2+, Sn2+ and inhibited by Zn2+, Cd2+, Fe2+, Co2+, Cu2+. Moreover, amino acid modifiers could inhibit differently its activity. The optimum pH of the enzyme reaction was 5.5.

用聚合酶链式反应(PCR)方法以短芽胞杆菌(Bacilusbrevis)基因组DNA为模板,克隆出0.97kb的DNA片段,经DNA测序证明是α-乙酰乳酸脱羧酶(α-ALDC)基因。将该基因重组到质粒pBV220中,构建了重组表达质粒pBVYI,转化大肠杆菌DH5α后,经筛选得到能表达α-ALDC活性的转化子细胞。酶活检测表明重组的α-ALDC的活性是供体菌的10000倍。从转化子细胞的抽提液中纯化了重组α-ALDC后,研究了其部分酶学特性。α-ALDC活性可被Mn2+、Sn2+增强,被Zn2+、Cd2+、Fe2+、Co2+、Cu2+抑制。氨基酸修饰剂对α-ALDC活性有不同程度的抑制作用。其最适pH值为5.5。

 
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