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小麦叶绿体     
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  wheat chloroplast
     CLONING AND EXPRESSION OF WHEAT CHLOROPLAST psbA GENE
     小麦叶绿体psbA基因的克隆及表达
短句来源
     Based on the sequenced wheat chloroplast genome (cpDNA), a pair of primers in infA-rp136 region was designed and used to amplify DNA from 12 diploid or polyploid Triticeae species. The 12 PCR products were cloned and sequenced. The resulting sequences ranged from 584 to 601bp.
     利用小麦叶绿体基因组中infA-rpl36区域的序列设计引物,对小麦族(Triticeae)的12个二倍体和多倍体的物种进行了PCR扩增和序列测定,获得了长度为584~603bp的12条DNA序列。
短句来源
     The effects of various concentrations of linolenic acid on the structure and the absorption and fluorescence spectra of wheat chloroplast membranes were studied.
     研究不同浓度的亚麻酸对小麦叶绿体膜结构、吸收光谱和荧光光谱的影响。
短句来源
     STUDIES ON BINDING ACTIVITY OF CYTOKININ-BINDING PROTEIN FROM WHEAT CHLOROPLAST
     小麦叶绿体中CTK结合蛋白的结合活性研究
短句来源
     The increasing of LOX activity in chloroplast and thylakoid membrane showed that there were correlative relationships among wheat chloroplast membrane damage under condition of water stress, membrane lipid peroxidation caused by free radicals, and oxide reaction caused by LOX.
     同时 ,叶绿体内和类囊体膜联结的LOX活性升高 ,表明水分胁迫对小麦叶绿体膜的损伤与自由基导致的膜脂过氧化和LOX导致的酶促氧化有关
短句来源
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  chloroplasts from wheat
     EFFECTS OF MG~(2+) ON PHOTOSYNTHETIC FUNCTIONS OF CHLOROPLASTS FROM WHEAT SEEDLING GROWN UNDER DIFFERENT LIGHT INTENSITIES
     Mg~(2+)对生长在不同光强下的小麦叶绿体光合功能的影响
短句来源
     Effects of Doubled_CO_2 Concentration on Ultrastructure, Supramolecular Architecture and Spectral Characteristics of Chloroplasts from Wheat
     CO_2 浓度倍增对小麦叶绿体超微结构、超分子结构及光谱特性的影响(英文)
短句来源
     EFFECTS OF ADDITIONAL FACTORS ON FLUORESCENT CHARACTERISTICS OF CHLOROPLASTS FROM WHEAT SEEDLINGS GROWN UNDER DIFFERENT LIGHT INTENSITIES
     外加因子对生长在不同光强下的小麦叶绿体荧光特性的影响
短句来源
     Effects of Mg2+ on photosynthetic functions of chloroplasts from wheat seedling grown under different light intensities were studied.
     研究结果表明,Mg~(2+)对生长在不同光强度下的小麦叶绿体光合功能有不同影响。
短句来源
  wheat chloroplasts
     Properties of a CTK-binding Protein from Wheat Chloroplasts
     小麦叶绿体中细胞分裂素结合蛋白的特性
短句来源
     EFFECTS OF WATER STRESS ON CHLOROPHYLL PROTEIN COMPLEXES IN WHEAT CHLOROPLASTS
     水分胁迫对小麦叶绿体色素蛋白复合体的影响
短句来源
     F. /F. and Fv/Fm ratios of wheat chloroplasts were decreased by pretreatment of chloroplasts with 10μ mol/1 DCMU at room temperature,but those of low-light wheat chloroplasts were decreased by DCMU according to a bigger margin.
     此外,在室温条件下,用10μmol/I DCMU预处理生长在不同光强下的小麦叶绿体,它们的F_v/F_o和F_m/F_o比值均下降,所不同的是,在低光强下生长的小麦,其叶绿体的这两个比值被降低的幅度更大.
短句来源
     Study on the Function of Cytokinin-Binding Protein in Wheat Chloroplasts
     小麦叶绿体中细胞分裂素结合蛋白的功能
短句来源
     A CTK-binding protein from wheat chloroplasts was shown to be heat-labile (Fig. 1 ).
     小麦叶绿体中的CTK结合蛋白属热敏感蛋白,它与6-BA结合的最适温度约为25℃;
短句来源
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  chloroplast from wheat
     Effect of Water Stress on Membrane Lipid Peroxidation of Chloroplast from Wheat
     水分胁迫对小麦叶绿体膜脂过氧化的影响
短句来源
     Effect of Water Stress on Photochemical Activity of Chloroplast from Wheat
     水分胁迫对小麦叶绿体光化学活性的影响
短句来源

 

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      wheat chloroplast
    A physical map of the Bromus inermis chloroplast genome was constructed using heterologous probes of barley and wheat chloroplast DNA (cpDNA) to locate restriction sites.
          
    Measurements of proton spin-lattice relaxation time T1 for thylakoid membranes of wheat chloroplast suspensions as a function of proton concentration in solvent are performed.
          
    The endpoints of an inversion in wheat chloroplast DNA are associated with short repeated sequences containing homology toatt-la
          
    The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence.
          
    The genes for the D2 and 44 kDa polypeptides are located close together, approximately 55 kbp from the gene for the 51 kDa polypeptide, in both pea and wheat chloroplast DNA.
          
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      chloroplasts from wheat
    The fragmentation of the LSU was observed when intact chloroplasts from wheat leaves were incubated under illumination in the presence of KCN or NaN3, which is a potent inhibitor of active oxygen-scavenging enzyme(s).
          
      wheat chloroplasts
    It was concluded that, in mature wheat chloroplasts, HS induced the formation of thylakoids characterized by a changed molecular structure and by increased lipid/protein and lipid/chlorophyll ratios.
          
    tRNAUCCGly, tRNAGGUThr, tRNAUUCGlu and tRNAGUATyr and an open reading frame of 62 codons have been identified by sequencing a 2,358 by BamHl and a 1,378 by BamHI-Sst2 DNA fragments from wheat chloroplasts.
          
    The apparent accumulation of ptDNA copies in wheat amyloplasts during endosperm development contrasts with the decline in chloroplast-DNA copies in wheat chloroplasts during leaf development.
          
    The effect of imposed watet stress on the development and ultrastructure of wheat chloroplasts
          
    Phytohormones like IAA and kinetin inhibit chlorophyll loss during aging of wheat chloroplasts duringin vivo andin vitro.
          
    更多          


    Chloroplasts isolated from wheat leaves in the presence of cofactors for photophosphorylation were illuminated with light of wave length shorter than 550mμ and the time course of their fluorescence decay was followed at 685mμ Addition of vitamin K_3 or PMS resulted in a general drop of fluorescence intensity in the homogenate and in the chloroplast suspension alone. The addition of vitamin K_3 or PMS brought the second peak into prominence (Fig. 1). No second peak appeared with cofactors of noncyclic photophosphorylation...

    Chloroplasts isolated from wheat leaves in the presence of cofactors for photophosphorylation were illuminated with light of wave length shorter than 550mμ and the time course of their fluorescence decay was followed at 685mμ Addition of vitamin K_3 or PMS resulted in a general drop of fluorescence intensity in the homogenate and in the chloroplast suspension alone. The addition of vitamin K_3 or PMS brought the second peak into prominence (Fig. 1). No second peak appeared with cofactors of noncyclic photophosphorylation such as ferricyanide system or NADP system. Addition of vitamin K_3 or PMS to the non-cyclic system brought the second peak into prominence (Figs. 6, 7 and 8). Inhibitors for oxygen liberation in photosynthesis such as o-phenanthroline, CMU and NH_2OH significantly inhibited the fluorescence decay (Figs. 2 and 3). DNP and NH_4C(?) strongly inhibited the appearance of the second peak (Figs. 4, 5 and 9).

    小麦叶绿体作为材料,测定叶绿素荧光685的时间进程中荧光强度变化,结果证明荧光强度变化与各种不同的光合磷酸化系统的辅助因素有关。光合作用放氧抑制剂对荧光衰变有抑制作用。磷酸化解偶联剂对第二波有抑制作用。在非循环的磷酸化系统中加入循环磷酸化的辅助因素后,所表现的荧光强度变化亦可转变。试验结果说明荧光衰变与放氧反应有联系。而第二波与循环或假循环磷酸化的偶联时能量转移有关系。

    The temperature effects on the CF_1-ATPase activity of the chloroplast fragments isolated from several higher plants, i.e., spinach (Spinacia oleracea L.), broadbean (Vicia faba L.), barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) were studied within the range of 0℃ to 40℃.

    本文是在已有工作基础上通过几种植物(菠菜、蚕豆、大麦和小麦)叶绿体片层膜上CF_1-ATP酶活力温度效应的研究,证明结合于膜的Mg~(++)-ATP酶活力受控于膜脂。游离的CF_1-ATP酶活力Arrhenius图上并无破折点出现,而结合于膜的Mg~(++)-ATP酶温度效应Arrhenius图上都有破折点出现。在测定温度范围内不同植物表现不同,菠菜的有两个破折点,蚕豆、大麦和小麦都只有一个破折点,彼此破折点所在的位置不同,活化能也不同。将菠菜叶绿体片层膜摘除CF_1后的残缺膜与蚕豆CF_1重组;或将蚕豆、大麦和小麦叶绿体制备的残缺膜片与菠菜的CF_1重组,其重组膜系统Mg~(++)-ATP酶温度效应Arrhenius图形都与各自的残缺膜原来膜片的Arrhenius图形相似。这些现象支持CF_1-ATP酶活力受控于膜脂。

    After a short-term solubilization with sodium dodecyl sulphate, chloroplast membranes of wheat were separated by discontinuous SDS-polyacrylamide gel electrophoresis into eight chlorophyll-containing zones. These pigment zones were designated, in order of increasing electrophoretic mobilities, CPI (P700 chlorophyll a-protein), LHCP~1 (Light-harvesting chlorophyll a/b-protein), LHCP~2, LHCP~3, CPa (The reaction center of photosystem Ⅱ),LHCP~4, CPa_1, and FC (Free chlorophyll complexed with SDS). It is to be noted...

    After a short-term solubilization with sodium dodecyl sulphate, chloroplast membranes of wheat were separated by discontinuous SDS-polyacrylamide gel electrophoresis into eight chlorophyll-containing zones. These pigment zones were designated, in order of increasing electrophoretic mobilities, CPI (P700 chlorophyll a-protein), LHCP~1 (Light-harvesting chlorophyll a/b-protein), LHCP~2, LHCP~3, CPa (The reaction center of photosystem Ⅱ),LHCP~4, CPa_1, and FC (Free chlorophyll complexed with SDS). It is to be noted that a new complex was observed between LHCP~4 and FC, which was named CPa_1.Absorption-spectra of CPa_1 is similar to that of CPa, they have peaks in the red region at 669 nm and 670 nm respectively, and in the blue region at 435 nm. Clearly these spectra resemble that of the complex Ⅳ-reaction center of PSⅡ (Hayden, et al., 1977). Fluorescence emission spectra of CPa and CPa_1 are similar, the emission maxima at 681 nm and 682 nm respectively. Fluorescence excitation spectra of CPa and CPa_1 also resemble one another. CPa has an apparent molecular weight of 39.5 KD, the CPa_1 has an apparent molecular weight of 19.6 KD. We presume that the CPa complex is probably a dimer and the CPa_1 complex is probably a monomer of the CPa.

    小麦叶绿体膜用SDS短时间增溶后,用不连续的SDS—聚丙烯酰胺凝胶电泳分离出八条叶绿素带,我们依其迁移率的增加及参考文献上的定名称为CPI(P700—叶绿素a—蛋白质)、LHCP~1(捕光叶绿素a/b—蛋白质)、LHCP~2、LHCP~3,CPa(光系统Ⅱ反应中心)、LHCP~4和FC(游离色素—SDS复合物)。值得注意的是,在LHCP~4和FC之间观察到一条新的复合体,我们命名为CPa_1。 CPa_1的吸收光谱与CPa的吸收光谱相似,他们在红区的吸收峰分别在669nm和670nm,在蓝区的吸收峰为435nm,清楚地表明这些吸收光谱与文献中报导的复合体Ⅳ—系统Ⅱ反应中心复合物相似(Hayden等1977)。CPa和CPa_1具有相似的荧光发射光谱,最强的发射带分别在681nm和682nm。二者的荧光激发光谱亦是彼此相似的。CPa的分子量约为39.5KD,CPa_1的分子量约为19.6KD。因此,我们推测CPa可能是二聚体,而CPa_1可能是它的单体。

     
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