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   家蚕细胞 在 畜牧与动物医学 分类中 的翻译结果: 查询用时:0.268秒
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家蚕细胞
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  bombyx mori cells
    Expression and Identification of Gene Coding for Sj23 Antigen in Bombyx mori Cells
    日本血吸虫中国大陆株Sj23抗原基因在家蚕细胞中的表达及鉴定
短句来源
    The construction of transfer vector,pBacPAK-His1-Sj23,is made through subcloning the Sj23 membrane protein gene from vector of pET28(c) into the transfer vector of pBacPAK-His1.The transfer vector pBacPaK-his1-Sj23 then co-trans-fected Bombyx mori cells with linealized virus of Bombyx mori nuclear polyhedrosis virus.
    将日本血吸虫 2 30 0 0膜蛋白 (Sj2 3抗原 )基因从原核表达载体 p ET2 8(c)中亚克隆入转移载体 p Bac PAK- His1,构建了 p Bac PAK- his1- Sj2 3转移载体 ,并与线性化家蚕杆状病毒共转染家蚕细胞 ;
短句来源
    SDS-PAGE shows that the (23 000) membrane protein was expressed in Bombyx mori cells,and the molecular weight is (26 000).
    SDS- PAGE显示日本血吸虫 2 30 0 0膜蛋白基因在家蚕细胞中实现表达 ,蛋白的相对分子质量为 2 6 0 0 0 ;
短句来源
  “家蚕细胞”译为未确定词的双语例句
    Expression of 45W-4B gene of Taenia solium in the silkworm
    猪带绦虫45W-4B基因在家蚕细胞中的表达
短句来源
    2. The pPGH030 and the linear Bm-BacPAK6 were co-transfected into the cell line of Bombyx mori (BmN), then the recombinant virus, Bm-BacPAK6-pgh was obtained successfully.
    2.将pPGH030 与线性化病毒Bm-BacPAK6 共转染家蚕细胞(BmN),成功获得了猪生长激素重组杆状病毒Bm-BacPAK6-pgh。
短句来源
    The above two plasmids and parental baculovirus Bm BacPAK6 DNA (digested with Cvn I) were used to cotransfect BmN cells, respectively. Recombinant viruses Bm vp1 and Bm vp2 were selected and purified by blue white plaque assay.
    以上两质粒分别与Cvn 酶切线性化的亲本病毒 Bm-Bac PAK6DNA共转染家蚕细胞 ,通过蓝白斑筛选 ,纯化得到重组病毒 Bm-vp1和 Bm-vp2。
短句来源
    The DNA of BmNPV(Bombyx mori Nucleopolyhedrovirus) which was purified with the method of preparation of virus DNA was spliced into linear DNA by the endorestrict enzyme, Bsu-1. Bombyx mori was cotransfected by the DNA of SJ23 together with the DNA of BmNPV.The recombinant virus was obtained through three cycles of screening. The target protein was shown by the SDS-PAGE and identified by Western-blot.
    同时纯化家蚕杆状病毒(BmNPV)的DNA,用限制性内切酶Bsu-1酶切成线性,与质粒DNA共转染家蚕细胞,经3轮纯化得到重组病毒;
短句来源
    Therecombinant BmNPV was screened, and used to infect Bm-N cells and silkworm.
    同时家蚕细胞能识别哺乳动物的分泌蛋白的信号肽序列。
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  bombyx mori cells
Heat shock response in Bombyx mori cells infected by nuclear polyhedrosis virus (NPV)
      
Silkworm (Bombyx mori) cells are characterized by the ability to synthesize heat shock proteins (hsps) at exceptionally high temperatures (up to 48° C).
      
When the Bombyx mori cells (Bm-N) were transfected with the pBmgp64Luc, different treatments were undertaken.
      


By cloning vp1 and vp2 genes of chicken anaemia virus into transfer vector pBacPAK8,recombinant transfer plasmids pBac\|vp1 and pBac\|vp2 were obtained. Then BmN cells were co\|transfected with linearized baculovirus Bm\|BacPAK6 DNA and above two recombinant plasmids respectively,recombinant viruses Bm\|vp1 and Bm\|vp2 were constructed and used to co\|infect silkworms to express recombinant proteins. The results indicated that recombinant VP1 and VP2 could induce the corresponding antibody in chickens...

By cloning vp1 and vp2 genes of chicken anaemia virus into transfer vector pBacPAK8,recombinant transfer plasmids pBac\|vp1 and pBac\|vp2 were obtained. Then BmN cells were co\|transfected with linearized baculovirus Bm\|BacPAK6 DNA and above two recombinant plasmids respectively,recombinant viruses Bm\|vp1 and Bm\|vp2 were constructed and used to co\|infect silkworms to express recombinant proteins. The results indicated that recombinant VP1 and VP2 could induce the corresponding antibody in chickens using immunofluorescence assay and the expression products could protect filial generation from the attack of CAV. Recombinant BmNPV expressing VP1 and VP2 is, therefore, a great hopeful production system for a subunit vaccine against CAV infection.

将鸡贫血病毒vp1和vp2基因分别克隆入转移载体pBacPAK8中 ,获得重组转移质粒pBac vp1和pBac vp2。以上两质粒分别与CvnⅠ酶切线性化的亲本病毒Bm BacPAK6DNA共转染家蚕细胞 ,通过蓝白斑筛选 ,纯化得到重组病毒Bm vp1和Bm vp2。PCR分析表明vp1和vp2基因已整合进杆状病毒基因组中。将Bm vp1和Bm vp2共感染 5龄家蚕 ,通过表达产物免疫SPF鸡产生的抗血清与CAV感染的MDCC MSB1细胞的间接荧光抗体分析 ,证明表达产物能诱导鸡产生相应的抗体 ,而且能够保护子代鸡免受CAV的攻击。该研究表明 ,表达VP1和VP2蛋白的重组家蚕杆状病毒 (RecombinantBmNPV)是很有前途的CAV亚单位疫苗的生产系统

VP1 and VP2 genes of chicken anaemia virus were cloned into transfer vector pBacPAK8, then recombinant transfer plasmids pBac vp1 and pBac vp2 were obtained, respectively. The above two plasmids and parental baculovirus Bm BacPAK6 DNA (digested with Cvn I) were used to cotransfect BmN cells, respectively. Recombinant viruses Bm vp1 and Bm vp2 were selected and purified by blue white plaque assay. Vp1 and vp2 genes were integrated into baculovirus genomes by PCR analysis. Silkworms were co infected with...

VP1 and VP2 genes of chicken anaemia virus were cloned into transfer vector pBacPAK8, then recombinant transfer plasmids pBac vp1 and pBac vp2 were obtained, respectively. The above two plasmids and parental baculovirus Bm BacPAK6 DNA (digested with Cvn I) were used to cotransfect BmN cells, respectively. Recombinant viruses Bm vp1 and Bm vp2 were selected and purified by blue white plaque assay. Vp1 and vp2 genes were integrated into baculovirus genomes by PCR analysis. Silkworms were co infected with Bm vp1 and Bm vp2. Antisera were obtained from SPF chickens inoculated with recombinant proteins. MDCC MSB1 cells infected with CAV reacted with the above antisera via immunofluorescence assay. The result showed the expression products could induce the corresponding antibody in chickens. Recombinant BmNPV expressing VP1 and VP2 is, therefore, a great hopeful production system for a subunit vaccine against CAV infection.

将鸡贫血病毒 VP1和 VP2基因分别克隆入转移载体 p Bac PAK8中 ,获得重组转移质粒 p Bac-vp1和 p Bac-vp2。以上两质粒分别与Cvn 酶切线性化的亲本病毒 Bm-Bac PAK6DNA共转染家蚕细胞 ,通过蓝白斑筛选 ,纯化得到重组病毒 Bm-vp1和 Bm-vp2。PCR分析表明 Vp1和 Vp2基因已整合进杆状病毒基因组中。将 Bm-vp1和 Bm-vp2共感染 5龄家蚕 ,通过表达产物免疫 SPF鸡产生的抗血清与 CAV感染的 MDCC-MSB1细胞的间接荧光抗体分析 ,证明表达产物能诱导鸡产生相应的抗体。该研究表明 ,表达 VP1和 VP2蛋白的重组家蚕杆状病毒 ( recombinant Bm NPV)是很有前途的CAV亚单位疫苗的生产系统

The construction of transfer vector,pBacPAK-His1-Sj23,is made through subcloning the Sj23 membrane protein gene from vector of pET28(c) into the transfer vector of pBacPAK-His1.The transfer vector pBacPaK-his1-Sj23 then co-trans-fected Bombyx mori cells with linealized virus of Bombyx mori nuclear polyhedrosis virus.The recombinant virus was got through PCR identification and the purified recombinant virus was got by blue and white plaque assay.SDS-PAGE shows that the (23 000) membrane protein was expressed...

The construction of transfer vector,pBacPAK-His1-Sj23,is made through subcloning the Sj23 membrane protein gene from vector of pET28(c) into the transfer vector of pBacPAK-His1.The transfer vector pBacPaK-his1-Sj23 then co-trans-fected Bombyx mori cells with linealized virus of Bombyx mori nuclear polyhedrosis virus.The recombinant virus was got through PCR identification and the purified recombinant virus was got by blue and white plaque assay.SDS-PAGE shows that the (23 000) membrane protein was expressed in Bombyx mori cells,and the molecular weight is (26 000).The recombinant Sj23 can be recognized by the serum of rabbit suffered from the S.japonicum.

将日本血吸虫 2 30 0 0膜蛋白 (Sj2 3抗原 )基因从原核表达载体 p ET2 8(c)中亚克隆入转移载体 p Bac PAK- His1,构建了 p Bac PAK- his1- Sj2 3转移载体 ,并与线性化家蚕杆状病毒共转染家蚕细胞 ;经 PCR鉴定得到重组病毒 ,经过蓝、白斑筛选得到纯化的重组病毒 ;SDS- PAGE显示日本血吸虫 2 30 0 0膜蛋白基因在家蚕细胞中实现表达 ,蛋白的相对分子质量为 2 6 0 0 0 ;重组 Sj2 3经 Western- Blot鉴定能够识别血吸虫感染多克隆兔血清

 
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