助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   家蚕细胞 在 蚕蜂与野生动物保护 分类中 的翻译结果: 查询用时:0.022秒
图标索引 在分类学科中查询
所有学科
蚕蜂与野生动物保护
生物学
基础医学
畜牧与动物医学
更多类别查询

图标索引 历史查询
 

家蚕细胞
相关语句
  bombyx mori cells
    EXPRESSION OF GREEN FLUORESCENT PROTEIN IN Bombyx mori CELLS
    绿色荧光蛋白在家蚕细胞中的表达
短句来源
    It was proved to be feasible and effective by our experiment using the cytoplasmic actin gene promotor and IE promotor to promote the expression of Green Fluorescent Protein in Bombyx mori cells.
    利用家蚕细胞质肌动蛋白基因(A3)启动子和NPV病毒即刻早期蛋白IE启动子在家蚕细胞中表达GFP,实验结果表明是可行与有效的。
短句来源
  silkworm cells
    Preliminary Study on the Infectivity of the Chemical Mutated Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) to Silkworm Cells and Larvae
    化学诱变的BmNPV对家蚕细胞和虫体感染性的初步研究
短句来源
    The Elementary Transgene Research of Silkworm Cells Mediated by Transposon piggyBac
    piggyBac转座子介导的家蚕细胞转基因研究初探
短句来源
    To investigate the effect of dsRNA on silkworm cells, we transfected three kinds of synthetic dsRNAs of 435bp(Api), 300bp(Ap2) and 399bp(AM) in length against the various regions of BmNPV's DNA polymerase gene and DNA helicase gene, which are indispensable for viral replication in silkworm cells by TransMessenger? transfection Reagent.
    本实验中我们在国际上首次应用BmNPV来研究RNAi,以BmNPV复制必需的DNA解旋酶基因和DNA聚合酶基因为靶序列,人工设计并合成了长度分别为435bp(Ap_1),300bp(Ap_2)和399bp(A_H)的三条dsRNAs,利用TransMessenger~(TM) transfection Reagent转染家蚕细胞,研究其对BmNPV复制的抑制效果。
短句来源
    Results indicated that in the experiment where silkworm cells were infected with wild-strain BmNPV of the three dsRNAs, Api and AH can effectively suppress the replication of virus, but Apt had no effect on the inhibition of viral replication. Api and AH can reduce the infective liter of BmNPV with a peak change of approximately 3-4 logs on day 4 post-infection.
    结果表明,在野生型病毒BmNPV感染家蚕细胞实验中,Ap_2和A_H这两个dsRNA能够有效的抑制病毒DNA的复制,并且在转染dsRNA后第四天抑制效果最佳,使病毒滴度(以空斑形成单位PFU/ml值表示)降低了10~3~10~4倍,而另外一个dsRNA(Ap_1)没有明显的抑制效果。
短句来源
    DNA dot blotting showed that total DNA of normal silkworm cells as a positive contrast had no hybrid signal; however, total DNA of infected cells, which transfected Ap2 or AH had a very weak hybrid Signal by contrast with the negative contrast (dot a).
    DNA斑点杂交结果表明作为阳性对照的正常家蚕细胞BmN的胞内总DNA无杂交信号,而分别转染了A_(p2)和A_H的家蚕细胞其内病毒DNA的含量与阴性对照相比减少了72.3%和63.4%。
短句来源
更多       
  silk worm cells
    Preliminary Study on the Infectivity of the Chemical Mutated Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) to Silkworm Cells and Larvae
    化学诱变的BmNPV对家蚕细胞和虫体感染性的初步研究
短句来源
    The Elementary Transgene Research of Silkworm Cells Mediated by Transposon piggyBac
    piggyBac转座子介导的家蚕细胞转基因研究初探
短句来源
    To investigate the effect of dsRNA on silkworm cells, we transfected three kinds of synthetic dsRNAs of 435bp(Api), 300bp(Ap2) and 399bp(AM) in length against the various regions of BmNPV's DNA polymerase gene and DNA helicase gene, which are indispensable for viral replication in silkworm cells by TransMessenger? transfection Reagent.
    本实验中我们在国际上首次应用BmNPV来研究RNAi,以BmNPV复制必需的DNA解旋酶基因和DNA聚合酶基因为靶序列,人工设计并合成了长度分别为435bp(Ap_1),300bp(Ap_2)和399bp(A_H)的三条dsRNAs,利用TransMessenger~(TM) transfection Reagent转染家蚕细胞,研究其对BmNPV复制的抑制效果。
短句来源
    Results indicated that in the experiment where silkworm cells were infected with wild-strain BmNPV of the three dsRNAs, Api and AH can effectively suppress the replication of virus, but Apt had no effect on the inhibition of viral replication. Api and AH can reduce the infective liter of BmNPV with a peak change of approximately 3-4 logs on day 4 post-infection.
    结果表明,在野生型病毒BmNPV感染家蚕细胞实验中,Ap_2和A_H这两个dsRNA能够有效的抑制病毒DNA的复制,并且在转染dsRNA后第四天抑制效果最佳,使病毒滴度(以空斑形成单位PFU/ml值表示)降低了10~3~10~4倍,而另外一个dsRNA(Ap_1)没有明显的抑制效果。
短句来源
    DNA dot blotting showed that total DNA of normal silkworm cells as a positive contrast had no hybrid signal; however, total DNA of infected cells, which transfected Ap2 or AH had a very weak hybrid Signal by contrast with the negative contrast (dot a).
    DNA斑点杂交结果表明作为阳性对照的正常家蚕细胞BmN的胞内总DNA无杂交信号,而分别转染了A_(p2)和A_H的家蚕细胞其内病毒DNA的含量与阴性对照相比减少了72.3%和63.4%。
短句来源
更多       
  “家蚕细胞”译为未确定词的双语例句
    Expression of Triplet Ribozyme Targeting Bombyx Mori Nuclear Polyhedrosis Virus (BmNPV) Immediate Early Gene mRNA in Bm-N Cell
    抗BmNPV即刻早期蛋白三联ribozyme在家蚕细胞中的表达
短句来源
    The Recombination and Expression of a Chimeric Antibody Light Chain Gene in Cells of Silkworm Bombyx mori
    嵌合抗体轻链基因在家蚕细胞中的重组与表达
短句来源
    Study on Apoptosis of BmN Cells Induced by CdCl_2
    CdCl_2诱导家蚕细胞(BmN)凋亡的研究
短句来源
    Using Flow cytometer (FCM) we found that the transfection efficiency of TransMessenger Reagent was 60-70%.
    结果发现TransMessenger~(TM) transfection Reagen的转染效率为60一70%,当用量超过16pl时,对家蚕细胞有毒副作用。
短句来源
    1. The inhibition effects of the corresponding dsRNA of ie-l on the replication and multiplication of BmNPVAccording to the gene sequence of ie-l in the BmNPV genome, the 438 bp long dsRNA was designed and synthesized in vitro and was transfected with BmNPV DNA into BmN cells by lipofectin.
    根据BmNPV的ie-1基因序列,人工设计并体外合成了长度为438 bp的dsRNA,利用脂质体转染家蚕细胞,研究其对BmNPV复制增殖的抑制效果。
短句来源
更多       
查询“家蚕细胞”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  bombyx mori cells
Heat shock response in Bombyx mori cells infected by nuclear polyhedrosis virus (NPV)
      
Silkworm (Bombyx mori) cells are characterized by the ability to synthesize heat shock proteins (hsps) at exceptionally high temperatures (up to 48° C).
      
When the Bombyx mori cells (Bm-N) were transfected with the pBmgp64Luc, different treatments were undertaken.
      
  silkworm cells
Site-specific gene integration in cultured silkworm cells mediated by φC31 integrase
      
The upstream region showed complete homology to the strong promoter of the AcMNPVp10, suggesting that this promoter from BmNPV could also be exploited for high-level expression of cloned foreign genes in silkworm cells or larvae.
      


e have contructed an universal transfer vector pUBM-4 for gene expression in silkworm.In this vector,the promoter is a strong one obtained from the polyhedrin ofBmNPV,Zhengjiang strain;5’flank sequence and 3’flank sequence of polyhedrin serve asthe homologous regions for in vivo recombination.ATG,the translation site of polyhedrin wasmutated to EcoRV site by PCR.The foreign egnes can be inserted into EcoRV site orBamHI site in this vector and expressed under the control of the polyhedrin promoter inBm-cells...

e have contructed an universal transfer vector pUBM-4 for gene expression in silkworm.In this vector,the promoter is a strong one obtained from the polyhedrin ofBmNPV,Zhengjiang strain;5’flank sequence and 3’flank sequence of polyhedrin serve asthe homologous regions for in vivo recombination.ATG,the translation site of polyhedrin wasmutated to EcoRV site by PCR.The foreign egnes can be inserted into EcoRV site orBamHI site in this vector and expressed under the control of the polyhedrin promoter inBm-cells and larvae.We have sucessfully expressed β-galactosidase gene with this vector insilkworm.The maximum level of expression is 580μg per larva,demonstrating that the transfervector pUBM-4 is reliable and useful.

本文从中国农科院蚕业研究所提供的我国家蚕核多角体病毒镇江株中获得了多角体蛋白的强启动子,用此启动子构建了家蚕病毒表达系统的转移载体。外源基因能在此启动子控制下在家蚕细胞和虫体中进行高效表达。用此载体我们首先成功地在家蚕虫体中高效表达了β-半乳糖苷酶,表达量达到580μg/条蚕,从而证实我们构建的载体是可靠的、有效的,可用于家蚕重组病毒表达外源基因的研究。

A chimerio immunoglobulin light-chain gene had been expressed in a baculovirus expression system,which was achieved by infecting the silkworm cells with the recombinant virus r-NPVL2 containing the cDNA of immunoglobulin against a small cell lung cancer.PCR analysis and Southern hybridization proved that the antibody light chain cDNA had been integrated into the modified BmNPV genome.Expression produets of human-mouse chimeric antibody light chain in the silkworm cells were detected by both Western blot and...

A chimerio immunoglobulin light-chain gene had been expressed in a baculovirus expression system,which was achieved by infecting the silkworm cells with the recombinant virus r-NPVL2 containing the cDNA of immunoglobulin against a small cell lung cancer.PCR analysis and Southern hybridization proved that the antibody light chain cDNA had been integrated into the modified BmNPV genome.Expression produets of human-mouse chimeric antibody light chain in the silkworm cells were detected by both Western blot and ELISA.

用家蚕修饰型核多角体病毒为载体,在家蚕细胞获得人鼠嵌合的抗入小细胞肺癌抗体轻链基因的表达.PCR和Southern杂交证明了抗体轻链基因已组建于家蚕病毒基因组中。Westernblot和ELISA分析都检测到在重组病毒感染的家委细胞中产生了人鼠嵌合的抗体轻链。

In this study, Marek's disease virus (MDV) Glycoprotein B(gB) gene was clonedinto transfer vector pBacPAK8 and the recombinant transfer vector pBacPAK (gB) wasobtained. The analysis with restriction endonuclease digestion and Southern blot showedthat gB gene was inserted into the transfer vector in the right direction and controlled bythe polyhedrin promoter. This recombinant transfer vector and parental virus Bm-Bac-PAK6 DNA (Cleaved with Cvn I ) were used to cotransfect Bm-N Cells with lipofectin.Only the...

In this study, Marek's disease virus (MDV) Glycoprotein B(gB) gene was clonedinto transfer vector pBacPAK8 and the recombinant transfer vector pBacPAK (gB) wasobtained. The analysis with restriction endonuclease digestion and Southern blot showedthat gB gene was inserted into the transfer vector in the right direction and controlled bythe polyhedrin promoter. This recombinant transfer vector and parental virus Bm-Bac-PAK6 DNA (Cleaved with Cvn I ) were used to cotransfect Bm-N Cells with lipofectin.Only the recombinant viruses could replicate in cells. After that, the recombinant virusplaque was selected and purified by blue-white plaque and dot hybridization assay. Thefifth instar larvae of Bombyx mori were injected with the purified recombinant virus.SDS-PAGE analysis of products from the recombinant virus infected silkworms showedthat MDV gB gene was expressed at high efficiency. The molecular weight of the ex-pressed products is mainly 97kD, the expressed level is 1 mg per ml hemolymph.

将马克克氏病毒(Marek'sdiseasevirus,MDV)糖蛋白B(gB)基因克隆入转移载体pBac-PAK8中,得到重组转移载体质粒pBacPAK(gB)。经限制性酶切图谱结合Southernblot分析鉴定表明,gB基因以正确方向插入转移载体,受多角体蛋白基因启动子控制。将此转移载体与经CvnI酶切线性化的亲本病毒Bm-BacPAK6DNA通过脂质体法共转梁家蚕细胞,只有发生重组的病毒才有复制增殖的能力。然后通过蓝白斑筛选、结合点杂交,纯化得到重组的空斑病毒vBM,用该重组病毒接种家蚕5龄幼虫,对表达产物进行SDS-PAGE分析,检测到gB基因在家蚕中高效表达,表达产物的分子量主要为97KD,表达量约为1mg/mL血淋巴。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关家蚕细胞的内容
在知识搜索中查有关家蚕细胞的内容
在数字搜索中查有关家蚕细胞的内容
在概念知识元中查有关家蚕细胞的内容
在学术趋势中查有关家蚕细胞的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社