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   家蚕细胞 在 基础医学 分类中 的翻译结果: 查询用时:0.011秒
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蚕蜂与野生动物保护
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家蚕细胞
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  silkworm cells
    High level expression of human granulocyte colony stimulating factor ( hG-CSF ) in silkworm cells and larvae
    人粒细胞集落刺激因子在家蚕细胞及幼虫中的高效表达
短句来源
    Expression of HBeAg Gene with Baculovirus Vector in Silk Worm Cells and its Expression Product Purification
    用杆状病毒载体在家蚕细胞中表达HBeAg基因及其表达产物纯化的研究
短句来源
    Expression of HBeAg Gene with Baculovirus Vector in Silk Worm Cells
    用杆状病毒载体在家蚕细胞中表达HBeAg基因
短句来源
    EXPRESSION OF SCHISTOSOMA JAPONICUN FATTY ACID BINDING PROTEIN GENE IN SILKWORM CELLS AND LARVAE *
    日本血吸虫脂肪酸结合蛋白基因在家蚕细胞及幼虫中的表达
短句来源
    AIM: To express the fatty acid binding protein ( Sj14FABP) gene of Schistosoma japonicun in the silkworm cells and larvae.
    目的: 在家蚕细胞和幼虫中表达日本血吸虫脂肪酸结合蛋白( Sj14) 基因。
短句来源
  “家蚕细胞”译为未确定词的双语例句
    Expression of Human Platelet Factor 4 in Cultured Cells and Larvae of Bombyx mori
    人血小板因子4在家蚕细胞和幼虫体内的表达
短句来源
    High Expression of ,HBV S Gene in Bm Cell and Silkworm
    乙肝病毒S基因在家蚕细胞及蚕体内高效表达
短句来源
    Expression of hepatitis E virus structural protein p166 gene fragment in BmN cell and silkworm pupa
    戊型肝炎病毒结构蛋白p166在家蚕细胞及蛹体中的表达
短句来源
    cDNA Cloning of Human Lactoferrin and its Expression in BmN Cells
    人乳铁蛋白cDNA的克隆及在家蚕细胞中的表达
短句来源
    Insertion of the hG-CSF. cDNA into BmNPV transfer vector pBE284 , formed a recombinant transfer vector pBE284-G. BmN cell was cotransfected with wild type viral DNA and pBE284-G.
    将hG-CSF基因插入家蚕核型多角体病毒(BmNPV)转移载体pBE284,经与野生型病毒DNA共转染家蚕细胞后,通过体内同源重组的方式构建了重组病毒BmNPV-G-CSF,Southern印迹杂交表明重组病毒DNA中含G-CSF基因片段。
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  silkworm cells
Site-specific gene integration in cultured silkworm cells mediated by φC31 integrase
      
The upstream region showed complete homology to the strong promoter of the AcMNPVp10, suggesting that this promoter from BmNPV could also be exploited for high-level expression of cloned foreign genes in silkworm cells or larvae.
      


utured

在1000ml滚瓶中,用微载体Cytodex3培养家蚕细胞,在合适的培养条件下(细胞接种浓度3.6×105细胞/ml、微载体浓度5g/L),5天后,细胞的终密度达2.8×106细胞/ml,细胞增长指数为7.9。在细胞指数生长期(48~60小时)用载有人α-干扰素(IFN-α)基因的重组病毒接种(感染复数为0.4PFU/细胞),5天后,培养上清中IFN-α效价为6.4×106IU/ml,是用方瓶培养的家蚕BmN细胞IFN-α的表达量的4倍。

The coding sequencese of the hepatitis B virus (adr) surface antigen were inserted in the genome of BmNPV to construct recombinant BmNPV which were used to infect silkworm cells larvae and pupae. The level of HBsAg synthsis in cell cultures infected with the recombinant BmNPV was estimated at 35.5ug/ml cell culture (106cell), 750ug per larva and 690ug per pupa. Western blot and electronmicroscope show that primarily purified products are 22nm (diameter) glycosylated particles. It's buoyant density is 1. 2g/ml...

The coding sequencese of the hepatitis B virus (adr) surface antigen were inserted in the genome of BmNPV to construct recombinant BmNPV which were used to infect silkworm cells larvae and pupae. The level of HBsAg synthsis in cell cultures infected with the recombinant BmNPV was estimated at 35.5ug/ml cell culture (106cell), 750ug per larva and 690ug per pupa. Western blot and electronmicroscope show that primarily purified products are 22nm (diameter) glycosylated particles. It's buoyant density is 1. 2g/ml which is consistent with that of HBsAg in the plasma of patients.

把人乙型肝炎病毒(adr)的表面抗原S基因插入到家蚕核型多角体病毒基因组中,构建了重组病毒BmNPVS。用重组病毒感染家蚕细胞,测得每毫升培养物(1×10~6细胞)HBsAg表达量达35.5μg;感染家蚕幼虫和蛹,经检测表明HBsAg产量平均为每头蚕约750μg,每只蛹约为690μg。初步纯化的表达产物经Western blotting和电镜观察证实,表达产物是直径为22nm的颗粒,并主要以糖基化形式存在。表达产物的浮力密度为1.2g/ml,与病人血清的HBsAg一致。

The cDNA of hG-CSF containing signal sequence was cloned into M13mp1 8 vector , and the sequence analysis showed that the cloned fragment was the high active form of hG-CSF. Insertion of the hG-CSF. cDNA into BmNPV transfer vector pBE284 , formed a recombinant transfer vector pBE284-G. BmN cell was cotransfected with wild type viral DNA and pBE284-G. The recombinant virus BmG-CSF was obtained by homologous recombination in vivo Southern-hybridization analysis suggests that the recombinant viral DNA contains...

The cDNA of hG-CSF containing signal sequence was cloned into M13mp1 8 vector , and the sequence analysis showed that the cloned fragment was the high active form of hG-CSF. Insertion of the hG-CSF. cDNA into BmNPV transfer vector pBE284 , formed a recombinant transfer vector pBE284-G. BmN cell was cotransfected with wild type viral DNA and pBE284-G. The recombinant virus BmG-CSF was obtained by homologous recombination in vivo Southern-hybridization analysis suggests that the recombinant viral DNA contains hG-CSF cDNA fragrnent. The biological activity of G-GSF was 1 . 2 × 1 06CFU /ml at day 4 in the supernatant of BmN cell culture infected with BmG-CSF and was 1 . 4× 107CFU /ml at day 4 in the hemolymph of silkworm larvae infected with BmG-CSF.

含信号肽序列的hG-CSFcDNA基因克隆于M13mp18中,测序表明其基因序列与高活性hG-CSFcDNA一致。将hG-CSF基因插入家蚕核型多角体病毒(BmNPV)转移载体pBE284,经与野生型病毒DNA共转染家蚕细胞后,通过体内同源重组的方式构建了重组病毒BmNPV-G-CSF,Southern印迹杂交表明重组病毒DNA中含G-CSF基因片段。重组病毒感染单层BmN细胞后第四天表达量达1. 2×106CFU/ml培养液,Western-blot分析可见分子量为19×103左右一条带。家蚕幼虫感染重组病毒后第四天表达量达1.4×107CFU/ml血淋巴(hemolymph)。

 
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