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   中性植酸酶 的翻译结果: 查询用时:0.241秒
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中性植酸酶
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  neutral phytase
     Six experimental diets PE610, PE615, PE810, PE815, PETT10, PET15 were formulated to contain 1 000 FTU/kg and 1 500 FTU/kg neutral phytase to replace 60%, 80%, 100% Ca (H_2PO_4)_2, respectively, and the control diet contains no neutral phytase.
     以(2.52±0.01)g的花鲈为试验对象,用中性植酸酶分别替代试验用的豆粕型饲料中60%(PE6)、80%(PE8)和100%(PET)的磷酸二氢钙,每个替代水平饲料中分别设置1000FTU/kg(PE610,PE810,PET10)和1500FTU/kg(PE615,PE815,PET15)2个梯度。
短句来源
     Neutral phytase producing strains were screened by laser compound mutagenesis,mixed wavelength 1.06+0.53 μm,irradiation time 1 min,irradiation power 15 W;
     采用激光复合诱变方式筛选产中性植酸酶的大肠杆菌(Escherichia coli)、枯草杆菌(Bacillus subtilis),混合波长λ=1.06+0.53μm,辐照时间1 m in,辐照功率15 W;
短句来源
     Cloning of Neutral Phytase Gene nphy from Bacillus subtilis and its Expression in Escherichia coli
     来源于Bacillus subtilis的中性植酸酶基因的克隆及在大肠杆菌中的表达
短句来源
     The properties,catalytic mechanism of neutral phytase were reviewed in this article,as well as its application in freshwater carp.
     本文综述了芽孢杆菌植酸酶的酶学性质、催化机制以及中性植酸酶在淡水鲤科鱼类饲料中的应用效果,并对其研究前景作了展望。
短句来源
     WT5BZ]The most adaptable pH value for the neutral phytase from seeding tomato before root growth is about 7.2. The activity of the phytase rises at temperature lower than 55 ℃.
     番茄种子萌发初期长根前产生的中性植酸酶的最适 pH在 7.2附近 ,在 55℃以内 ,酶活性随温度升高而增大 .
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  “中性植酸酶”译为未确定词的双语例句
     Based on two primers 5BAP01 containing a BamHI site and 3BAP01 containing a Xhol site, the pGEM? T Easy BAP Vector was double digested with BamHI and Xhol, and then cloned into the expression vector pET-30a(+) previously digested with the same enzymes. The resulting plasmid BAPpET30a(+)Vector was introduced into E.
     通过带限制性内切酶位点的引物(5BAP01带BamHI位点,3BAP01带XhoI位点),将淀粉液化芽孢杆菌编码的中性植酸酶基因定向插入到原核表达载体pET-30a(+)上,得到重组表达质粒BAP-pET-30a(+)Vector。
短句来源
     A pair of expression primers, the sense primer 5BAPK01 containing a SnaBI site and antisense primer 3BAPK01 containing a NotI site were designed according to the Multiple Cloning Sites(MCS) of expression vector pPIC9K and the Open Reading Frame of BA gene. The PCR fragment were cloned into pGEM?
     根据酵母表达载体pPIC9K的多克隆位点和已克隆的中性植酸酶基因限制性内切酶图谱,设计具有SnaBI位点的上游引物5BAPK01和具有NotI位点的下游引物3BAPK01。
短句来源
     Optimal pH value and temperature of the phytase for its activity were 7.5 and 55℃,respectively. The K m values of the phytase for dodecasodium phytate under 37℃ was 0.19mmol/L.
     此中性植酸酶的反应最适 pH为 7 5,最适温度为 55℃ ,在 37℃下以植酸钠为底物的Km值为 0 1 9mmol/L ,植酸酶活性依赖Ca2 +的存在。
短句来源
     Overexpression of Phytase Gene From B.amyloliquefaciens in Escherichia coli and Pichia Pastoris
     淀粉液化芽孢杆菌中性植酸酶基因在大肠杆菌和毕赤酵母中的高效表达
短句来源
     Scientists found Bacillus phytase has the higher thermostablity than Aspergillus phytase. Moreover, its optimum reaction pH is between 6.5 and 7.5, which makes it be used carp with neutral intestinal tract.
     研究表明,来源于芽孢杆菌的植酸酶属于中性植酸酶不仅具有较好的热稳定性,有助于抵抗饲料制粒或者膨化过程中引起的酶失活,而且其酶促反应的pH有效作用范围在6.5~7.5之间,适合用于消化道呈中性的鲤科鱼类,以及在单胃动物中pH值呈中性的肠道段中表现酶活力。
短句来源
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  相似匹配句对
     PURIFICATION AND PROPERTIES OF NEUTRAL PHYTASE FORM \%BACILLUS SUBTILIS'
     枯草芽孢杆菌中性植酸酶的纯化和酶学性质
短句来源
     Neutral doctrism
     中性主义
短句来源
     Screening Neutral Phytase Producing Strains by Laser Compound Mutagenesis
     中性植酸酶产生菌的激光复合诱变筛选
短句来源
     Neuter Times
     中性时代
短句来源
     A study of phytase and its application
     植酸酶及其应用
短句来源
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WT5BZ]The most adaptable pH value for the neutral phytase from seeding tomato before root growth is about 7.2. The activity of the phytase rises at temperature lower than 55 ℃. Its K m and V max are 104 μmol/L and the 3.45 nmol/(min·L) respectively. The neutral phytase is inhibited remarkably by Ca 2+ , Mg 2+ , Cu 2+ and Zn 2+ , not effected by Na + and K +, and maintains higher activity at pH5.0.

番茄种子萌发初期长根前产生的中性植酸酶的最适 pH在 7.2附近 ,在 55℃以内 ,酶活性随温度升高而增大 .其Km 是 10 4 μmol/L ,Vmax是 3.4 5nmol/ (min·L) .一价的Na和K离子对酶活性无影响 .二价的Ca、Mg、Cu和Zn离子有抑制酶活性作用 .酶保存在pH5.0时活性维持时间长

The gene encoding the neutral phytase nphy was cloned from Bacillus subtilis by polymerase chain reaction(PCR). Nucleotide sequence analysis of nphy revealed the presence of an open reading frame of 1152 bp coding for 383 aa. The start codon was followed by a sequence coding for a putative signal peptide of 26 aa in length. The nphy without original signal peptide encoding sequence was cloned into E.coli expression plasmid pTYB40. The result of SDS PAGE of the phytase expressed in E.coli...

The gene encoding the neutral phytase nphy was cloned from Bacillus subtilis by polymerase chain reaction(PCR). Nucleotide sequence analysis of nphy revealed the presence of an open reading frame of 1152 bp coding for 383 aa. The start codon was followed by a sequence coding for a putative signal peptide of 26 aa in length. The nphy without original signal peptide encoding sequence was cloned into E.coli expression plasmid pTYB40. The result of SDS PAGE of the phytase expressed in E.coli showed that the nphy had been overexpressed. The expressed phytase was over 40% of the total soluble protein of E.coli ,and has normal bioactivity.

通过PCR的方法从Bacillussubtilis基因组中克隆了中性植酸酶基因nphy ,DNA全序列分析表明其结构基因全长 115 2个核苷酸 (编码 383个氨基酸 ) ,5′端有一编码 2 6个氨基酸的信号肽序列。去除信号肽编码序列的nphy克隆到大肠杆菌IPTG诱导表达载体pTYB40上 ,在大肠杆菌中得到了高效表达 ,表达量达到大肠杆菌可溶性蛋白的 40 %以上 ,表达产物具有生物学活性 ,证实了克隆到的中性植酸酶的编码基因有正常的生物学功能。

A strain Bacillus subtilis producing neutral phytase was screened from soil.The protein of phytase was purified by HPLC.Optimal pH value and temperature of the phytase for its activity were 7.5 and 55℃,respectively.The K m values of the phytase for dodecasodium phytate under 37℃ was 0.19mmol/L.The molecule weight of the phytase protein was determined as about 45kD by SDS\|PAGE.The N\|terminal amino acids sequence of the phytase protein was determined as Lys\|His\|Lys\|Leu\|Ser\|Asp\|Pro\|Tyr\|His\|Phe\|Thr...

A strain Bacillus subtilis producing neutral phytase was screened from soil.The protein of phytase was purified by HPLC.Optimal pH value and temperature of the phytase for its activity were 7.5 and 55℃,respectively.The K m values of the phytase for dodecasodium phytate under 37℃ was 0.19mmol/L.The molecule weight of the phytase protein was determined as about 45kD by SDS\|PAGE.The N\|terminal amino acids sequence of the phytase protein was determined as Lys\|His\|Lys\|Leu\|Ser\|Asp\|Pro\|Tyr\|His\|Phe\|Thr by amino acids sequence analysis.

从土壤中分离到了产中性植酸酶的枯草芽孢杆菌菌株并对所产植酸酶进行了分离纯化。此中性植酸酶的反应最适 pH为 7 5,最适温度为 55℃ ,在 37℃下以植酸钠为底物的Km值为 0 1 9mmol/L ,植酸酶活性依赖Ca2 +的存在。酶蛋白的分子量大小约为 45kD ,纯酶蛋白N端序列为Lys His Lys Leu Ser Asp Pro Tyr His Phe Thr。

 
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