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   中国旱獭 的翻译结果: 查询用时:0.201秒
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中国旱獭
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  chinese marmot
     Results The results indicate that Chinese marmot IFN-α gene is a multigenic family from 8 IFNA functional sequences and 4 pseudogene sequences.
     结果从112个中国旱獭IFNα基因克隆中获得18个独立的不重复序列,其中的14个序列来自4次以上相对独立的PCR产物,将它们分为14个基因亚型,其中8个是功能基因亚型,6个是假基因亚型。
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     Expression of interferon alpha family gene of Chinese marmot in eukaryotic and prokaryotic cells
     中国旱獭干扰素α家族基因在真核细胞和原核细胞中的表达
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     Cloning and Sequence Analysis of Chinese Marmot Interleukin-10
     中国旱獭IL-10分子的克隆和序列分析
短句来源
     Conclusion The IFNα family gene of the Chinese marmot can be expressed in both eukaryotic and prokaryotic cells, and the expression products show antiviral activity in a protection assay.
     结论中国旱獭IFNα家族基因能在真核和原核细胞中表达,表达产物具有生物学活性。
短句来源
     The overall identity of the amino acid sequence among the members of the Chinese marmot IFN-α family is 85%, and the identity with the IFN-α family from other species such as mice and humans is 53%.
     中国旱獭IFNα各基因亚型之间在核苷酸水平和氨基酸水平有很高的同源性,平均分别为93%和85%,前体蛋白N端含23个氨基酸的疏水性信号肽。
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  “中国旱獭”译为未确定词的双语例句
     Objective: To construct the prokaryotic plasmids expressing the carbohydrate recognition domain(CRD) of the H1 and H2 subunits of asialoglycoprotein receptor(ASGPR) of Marmota himalayan,express and purify the recombinant proteins, develop the polyclonal antibodies against the CRDH1 or CRDH2 of ASGPR.
     目的:构建中国旱獭去唾液酸糖蛋白受体(ASGPR)H1和H2亚基糖基识别域(CRD)的原核表达质粒,体外表达纯化后制备多克隆抗体。
短句来源
     Methods: ASGPR CRDH1 or CRDH2 was amplified by RT-PCR from the liver of Marmota himalayan and cloned into prokaryotic expression vector pRSET-B, then expressed in E. coli BL21(DE3) pLysS. The recombinant proteins were purified using Ni2+-NTA spin column.
     方法:RT-PCR扩增出中国旱獭肝组织中ASGPR CRDH1和CRDH2 cDNA,将其克隆至原核表达载体pRSET-B中,在大肠杆菌BL21(DE3)pLysS内诱导表达。
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     Prokaryotic Expression, Purification and Renaturation of CRDH1 and CRDH2 of Chinese Marmata Asialoglycoprotein Receptor
     中国旱獭去唾液酸糖蛋白受体H1和H2亚基糖基识别域的原核表达及复性
短句来源
     Cloning,prokaryotic expression and polyclonal antibody preparation of the asialoglycoprotein receptor of marmota himalayan
     中国旱獭去唾液酸糖蛋白受体糖基结合域的原核表达与多克隆抗体的制备
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     We detected the biological activity of all expression products by viral protection assay, and analyzed their differences and species restriction of the biological activity.
     病毒保护实验检测表达产物的生物学活性,比较不同基因亚型IFNα生物学活性的差异,分析中国旱獭IFNα生物学活性的种属特异性。
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     Cloning and Sequence Analysis of Chinese Marmot Interleukin-10
     中国旱獭IL-10分子的克隆和序列分析
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Objective To investigate the function of interferon alpha (IFNα) in a Chinese marmot model of hepatitis B, we expressed the Chinese marmot (Marmota himalayana) IFNα family gene (IFNA) in eukaryotic cells and prokaryotic cells. Methods Eukaryotic and prokaryotic expression plasmids harboring Chinese marmot interferon alpha gene with different genotypes were generated using molecular cloning technology. We detected the biological activity of all expression products by viral protection assay, and analyzed their...

Objective To investigate the function of interferon alpha (IFNα) in a Chinese marmot model of hepatitis B, we expressed the Chinese marmot (Marmota himalayana) IFNα family gene (IFNA) in eukaryotic cells and prokaryotic cells. Methods Eukaryotic and prokaryotic expression plasmids harboring Chinese marmot interferon alpha gene with different genotypes were generated using molecular cloning technology. We detected the biological activity of all expression products by viral protection assay, and analyzed their differences and species restriction of the biological activity. Results The Chinese marmot functional genotype IFNα was expressed in the baby hamster kidney (BHK) cell line, and these products protected WH12/6 cells challenged by encephalomyocarditis virus (EMCV). The Chinese marmot IFN-α5 also expressed in E.Coli induced by IPTG, and purified fusion protein had antiviral biological activity. The biologic activity displayed differences among different subtype IFNα, and it had strict species restriction. Conclusion The IFNα family gene of the Chinese marmot can be expressed in both eukaryotic and prokaryotic cells, and the expression products show antiviral activity in a protection assay. This study provides, for the first time, evidence that IFNα from the Chinese marmot has an antiviral function in vitro and can be used to improve the efficacy of current therapies for HBV infection in our Chinese marmot model.

目的将不同基因型中国旱獭干扰素α(IFNα)基因在真核和原核细胞中进行表达,检测其生物学活性,以期利用克隆的中国旱獭IFN在动物模型上探索慢性乙型肝炎有效的IFN治疗方案和策略。方法利用分子克隆技术将14个不同亚型的中国旱獭IFNα家族基因亚克隆到真核表达载体,将中国旱獭IFNα5亚型基因亚克隆到原核表达载体。病毒保护实验检测表达产物的生物学活性,比较不同基因亚型IFNα生物学活性的差异,分析中国旱獭IFNα生物学活性的种属特异性。结果 14个基因亚型中国旱獭IFNα中,8个功能基因亚型真核表达产物有生物学活性,而6个假基因亚型产物没有生物学活性,8个功能基因亚型真核表达产物生物学活性存在差异,且其活性都受种属特异性限制。原核表达纯化产物具有较好的生物学活性。结论中国旱獭IFNα家族基因能在真核和原核细胞中表达,表达产物具有生物学活性。本研究为在中国旱獭乙型肝炎病毒感染模型上探索IFN治疗策略奠定了实验基础。

Objective To investigate the function of interferon alpha(IFN-α) in the Chinese marmot(Marmota himalayana), an animal model of hepatitis B. Methods Plasmids harboring Chinese marmot interferon alpha gene (IFNA) with different genotype were generated with molecular cloning technology. Sequence was sequenced, subtyped and put up homologous analysis and phylogenetic analysis. Results The results indicate that Chinese marmot IFN-α gene is a multigenic family from 8 IFNA functional sequences and 4 pseudogene sequences....

Objective To investigate the function of interferon alpha(IFN-α) in the Chinese marmot(Marmota himalayana), an animal model of hepatitis B. Methods Plasmids harboring Chinese marmot interferon alpha gene (IFNA) with different genotype were generated with molecular cloning technology. Sequence was sequenced, subtyped and put up homologous analysis and phylogenetic analysis. Results The results indicate that Chinese marmot IFN-α gene is a multigenic family from 8 IFNA functional sequences and 4 pseudogene sequences. The overall identity of the amino acid sequence among the members of the Chinese marmot IFN-α family is 85%, and the identity with the IFN-α family from other species such as mice and humans is 53%. Conclusion We creat a tool to test the antiviral effect of new forms of IFN-α delivery and to improve the efficacy of current therapies in Chinese marmot HBV model.

目的克隆中国旱獭α干扰素(IFNα)家族基因,以期利用克隆的中国旱獭家族IFNα在动物模型上探索慢性乙型肝炎有效的干扰素治疗方案和策略。方法利用分子克隆技术对中国旱獭IFNα家族基因进行克隆,并对所克隆的系列基因进行测序、分型、系统发生树分析、同源性比较及理化特性分析。结果从112个中国旱獭IFNα基因克隆中获得18个独立的不重复序列,其中的14个序列来自4次以上相对独立的PCR产物,将它们分为14个基因亚型,其中8个是功能基因亚型,6个是假基因亚型。中国旱獭IFNα各基因亚型之间在核苷酸水平和氨基酸水平有很高的同源性,平均分别为93%和85%,前体蛋白N端含23个氨基酸的疏水性信号肽。结论中国旱獭IFNα家族至少有14个基因亚型,这些基因的克隆可能应用于中国旱獭HBV动物感染模型,进行干扰素基因治疗和研究干扰素治疗策略。

Objective The aim of this study is to construct prokaryotic plasmids expressing carbohydrate recognition domain (CRD) genes—subunit H1 (CRDH1) and subunit H2 (CRDH2) of Chinese parmota asialoglycoprotein receptor (ASGPR), purify and renature the recombinant proteins CRDH1 and CRDH2. Methods Both CRDH1 and CRDH2 of ASGPR were amplified by RT-PCR from the liver tissues of Chinese marmota and cloned into the prokaryotic vector pRSET-B, respectively. Expression of the 6xHis-tagged recombinant proteins was induced...

Objective The aim of this study is to construct prokaryotic plasmids expressing carbohydrate recognition domain (CRD) genes—subunit H1 (CRDH1) and subunit H2 (CRDH2) of Chinese parmota asialoglycoprotein receptor (ASGPR), purify and renature the recombinant proteins CRDH1 and CRDH2. Methods Both CRDH1 and CRDH2 of ASGPR were amplified by RT-PCR from the liver tissues of Chinese marmota and cloned into the prokaryotic vector pRSET-B, respectively. Expression of the 6xHis-tagged recombinant proteins was induced by IPTG in E. coli BL21(DE3)pLysS. Purification of the recombinant proteins was performed by affinity chromatography with Ni-NTA His-bind resin. The purified recombinant proteins were then renatured in dialysis buffer. Results The recombinant proteins ASGPR CRDH1 and CRDH2 were efficiently expressed as inclusion bodies in prokaryotic cells. The final purified products of DRDH1 and CRDH2 had the molecular weight of 22 ku and 15 ku correspondingly. By using mouse monoclonal antibody against ASGPR (8D7), Dot-ELISA proved that both CRDH1 and CRDH2 recombinant proteins were effectively renatured. Conclusion The purified and renatured recombinant proteins ASGPR CRDH1 and CRDH2 presented in this study may be used as targeting molecules for gene therapy of liver diseases.

目的对中国旱獭去唾液酸糖蛋白受体(ASGPR)H1和H2亚基糖基识别域(CRD)的克隆、表达、纯化及复性。方法用RTPCR从中国旱獭肝组织中扩增ASGPRCRDH1和CRDH2cDNA,分别将其克隆到原核表达载体pRSETB上,在埃希菌BL21(DE3)pLysS内诱导表达含6个组氨酸标签的融合蛋白。融合蛋白经Ni2+螯合柱亲和纯化后,在体外行透析复性。结果ASGPRCRDH1和CRDH2经原核表达后得到分子量约为22ku和15ku的目的蛋白,以包涵体形式存在。经Ni2+螯合柱亲和纯化后获得纯度大于95%的融合蛋白。利用仅识别天然构像的单克隆抗体对复性后产物进行检测,证明复性成功。结论成功地表达了具有活性的ASGPRCRDH1和CRDH2的融合蛋白,在肝脏疾病的靶向治疗研究中具有潜在的应用价值。

 
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