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增殖细胞
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  proliferating cell
    Methods Apoptotic cells in the glomeruli have been analyzed by the in situ end labeling (ISEL) of TdT from patients diagnosed with membranoproliferative glomerulonephritis (MPGN, n = 12), type IV lupus nephritis (LN, n = 15) and acute postinfectious glomerulonephritis (APGN, n = 13) in the present study. In addition, the proliferating cell nuclear antigen (PCNA)-positive proliferating glomerular cells were observed by immunochemical 4 layer PAP method in these patients.
    方法 用原位末端标记方法对12例MPGN,15例LN和13例APGN患者肾活检组织中肾小球内凋亡细胞进行了观察,并用免疫组化方法同时对增殖细胞(PCNA染色阳性细胞)进行了分析。
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    Objective To study DNA ploidy, AgNORS count and proliferating cell nuclear antigen (PCNA) labeling index of prostatic carcinoma (PCa) and determine the relationships between these biologic markers and tumor grade and prognosis of the carcinoma.
    目的探讨前列腺癌细胞核DNA倍体、核仁组成区嗜银蛋白(AgNORS)计数及增殖细胞抗原(PCNA)阳性指数变化与癌分级及预后的关系。
短句来源
    Methods: 28 cases were divided into three groups,non-proliferative GN group 8 cases,proliferative GN group 12 cases,and proliferative GN with glomerulosclerosis group 8 cases. Their renal biopsy specimens were separately examined by using in situ end labeling(ISEL) to detect apoptosis cell and by immunohistochemistry to detect proliferating cell.
    方法:将非增生性肾炎8例、增生性肾炎12例及伴肾小球硬化的增生性肾炎8例病人的肾活检标本,采用原位末端标记方法并结合光镜形态学检查检测凋亡细胞,用免疫组化方法检测增殖细胞核抗原(PCNA)阳性的增殖细胞
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  “增殖细胞”译为未确定词的双语例句
    The clinical significance of DNA ploidy S-phase fraction and proliferation index determination in nephroblastoma
    肾母细胞瘤组织中DNA倍体、SPF、增殖细胞指数的测定
短句来源
    Results The proliferation index(PI) and the apoptosis index(AI) of BPH and Pca were much higher than that of NP ( P <0.01) and the AI/PI much lower( P <0.01). The PI and AI of Pca were much higher than that of BPH ( P <0.01) and the AI/PI much lower ( P <0.01).
    结果 BPH和Pca的增殖细胞指数 (PI)及凋亡细胞指数 (AI)较NP明显增高 (P <0 .0 1) ,但AI/PI却显著下降 (P <0 .0 1) ,其中Pca的PI和AI又较BPH明显增高 (P <0 .0 1) ,而AI/PI却明显下降 (P <0 .0 1)。
短句来源
    The number of apoptotic glomemlar cells was significantly higher in patients with APGN (1.16±0.35) than those with MPGN (0.12±0.16) as well as LN patients (0.35 ± 0.20).
    凋亡细胞数在APGN明显大于MPGN和LN,而增殖细胞与凋亡细胞的比值则以MPGN最大。
短句来源
    Glomerular apoptosis score was significantly higher in proliferative GN with glomerulosclerosis group than proliferative GN group( P <0.05).
    增生性肾炎肾小球凋亡细胞数显著低于伴肾小球硬化的增生性肾炎(P<0.05); 伴肾小球硬化的增生性肾炎肾小球增殖细胞数低于凋亡细胞数,但差异无显著性(P>0.05)。
短句来源
    Methods Theapoptosis and proliferation of p53 and bcl - 2 genic protein in 44 patients with transitionalcell bladder carcinomas were observed by TdT dUTP nick end labelling(TUNEL) andimmunohistochemical staining. ResuIts The density of apoptotic cells and proliferatic cellsin bladder carcinomas were elevated with histologic grading.
    方法采用DNA缺口末端标记(TUNEL)技术和免疫组织化学方法检测44例膀跳癌中细胞凋亡与增殖及p53、bcl-2基因蛋白的表也结果膀胱癌凋亡、增殖细胞密度随组织学分级增高均呈上升趋势;
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  proliferating cell
Special attention is given to the regulation of excision repair by different proteins-proliferating cell nuclear antigen (PCNA), p53, and proteasome.
      
Synthesis with eukaryotic DNA polymerases α, δ, and ε involves various replication factors, including the replication protein A, replication factor C, proliferating cell nuclear antigen, etc.
      
This is done by forming complexes of polymerase δ or ? with proliferating cell nuclear antigen (PCNA) and replication factors RP-A and RF-C.
      
The endothelium proliferation was evaluated using antibodies to proliferating cell nuclear antigen.
      
The obtained data indicate an important role of cardiomyocyte polyploidy and of activation of the proliferating cell nuclear antigen in development of myocardial hypertrophy in patients with OHCMP.
      
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Experimental diffuse tubulointerstitial fibrosis was rapidly induced by UUO in mice. By the 15th day of UUo, the tubulointerstitium showed extensive epithelial cell necrosis, inflammatory cell infiltration and interstitial volume expansion. By the 18th day of UUO, focal cellular proliferation occured in renal interstitium and then replaced by extensive cellular proliferation at the 21st day of UUO, By the 24th day of UUO, the renal interstitium showed diffuse fibrosis. These proliferative cells in renal interstitium...

Experimental diffuse tubulointerstitial fibrosis was rapidly induced by UUO in mice. By the 15th day of UUo, the tubulointerstitium showed extensive epithelial cell necrosis, inflammatory cell infiltration and interstitial volume expansion. By the 18th day of UUO, focal cellular proliferation occured in renal interstitium and then replaced by extensive cellular proliferation at the 21st day of UUO, By the 24th day of UUO, the renal interstitium showed diffuse fibrosis. These proliferative cells in renal interstitium were identified as fibroblasts by immunohistochemical stain. The result suggested that fibroblasts may play an important role in tubulointerstitial fibrogenesis.

本文应用单侧输尿管便阻的方法制备小鼠肾间质纤维化模型。小鼠输尿管梗阻后15d,肾小管上皮细胞出现大量坏死,间质炎细胞浸润,肾间质面积增宽;术后21d,肾小管和间质的结构基本消失,肾间质呈弥漫性细胞增殖;24d,肾间质中增殖的细胞数量减少,出现广泛的纤维化。用免疫组化法证实肾间质中增殖的细胞是成纤维细胞。本模型的建立为研究肾间质纤维化的发生发展提供了一种快速、可靠的方法

To facilitate an understanding of the relationship between bening prostatie hyperplasia(BPH) and extracelluar matrix (ECM ), the BPH model of rat had been funded and the immunolocalization of fiberonectin(FN), type IV collagen (COL IV)was detected with immunohistochemistry. The results showed the rate of strong immunostaining of FN, COL IV in testosterone group were significantly higher than those in other group a association was found between the expression of FN, COL IV and the weight of rat BPH. these data...

To facilitate an understanding of the relationship between bening prostatie hyperplasia(BPH) and extracelluar matrix (ECM ), the BPH model of rat had been funded and the immunolocalization of fiberonectin(FN), type IV collagen (COL IV)was detected with immunohistochemistry. The results showed the rate of strong immunostaining of FN, COL IV in testosterone group were significantly higher than those in other group a association was found between the expression of FN, COL IV and the weight of rat BPH. these data confirmed that ECM component in BPH was increased, and ECM might be involved in the development of BPH by help of steroid hormones.

为了研究性激素及细胞外基质在良性前列腺增生(BPH)发生中的相互作用,实验建立大鼠BPH模型,并采用免疫组织化学ABC法对大鼠前列腺组织中的纤维连续蛋白(FN),Ⅳ型胶原蛋白(COL IV)进行表达,发现睾丸素组的前列腺重量明显增加,FN及COL IV强阳性染色率显著高于去势组及对照组(P<0.01,0.05),而且前列腺的重量分别与FN,COL IV表达呈正相关(P<0.05)。提示在大鼠BPH模型中细胞外基质成分增加,增殖的细胞与大量积聚的细胞外基质共同作用导致BPH的发生。

Objective Hypercellularity in proliferan've glomerulonephritis can result from either increased proliferation or the failure of cells to undergo apoptosis in response to stimuli. Increasing evidence supports that alterations in the control of cell survival are important in the pathogenesis of proliferative glomerulonephritis. Methods Apoptotic cells in the glomeruli have been analyzed by the in situ end labeling (ISEL) of TdT from patients diagnosed with membranoproliferative glomerulonephritis (MPGN, n = 12),...

Objective Hypercellularity in proliferan've glomerulonephritis can result from either increased proliferation or the failure of cells to undergo apoptosis in response to stimuli. Increasing evidence supports that alterations in the control of cell survival are important in the pathogenesis of proliferative glomerulonephritis. Methods Apoptotic cells in the glomeruli have been analyzed by the in situ end labeling (ISEL) of TdT from patients diagnosed with membranoproliferative glomerulonephritis (MPGN, n = 12), type IV lupus nephritis (LN, n = 15) and acute postinfectious glomerulonephritis (APGN, n = 13) in the present study. In addition, the proliferating cell nuclear antigen (PCNA)-positive proliferating glomerular cells were observed by immunochemical 4 layer PAP method in these patients. Results The ratio of proliferating and apoptotic cells was the highest one in patients with MPGN (3.91) as compared to APGN (1.28) and LN(1 .02). The extends of increasing apoptotic cells in the glomeruli were different among patients with MPGN,, APGN and LN, although all of them showed extremely glomerular hypercellularity. The number of apoptotic glomemlar cells was significantly higher in patients with APGN (1.16±0.35) than those with MPGN (0.12±0.16) as well as LN patients (0.35 ± 0.20). Conclusion Apoptosis is essential in the resolution of glomerular cellularity together with the remodeling processes. Intense proliferations without increment in apoptosis are the characterisitics of MPGN and LN associated with their renal histological changes and prognosis.

目的 观察细胞增殖与凋亡在膜增生性肾小球肾炎(MPGN)、弥漫增生性狼疮性肾炎(LN)和急性感染后肾炎(APGN)的表现。方法 用原位末端标记方法对12例MPGN,15例LN和13例APGN患者肾活检组织中肾小球内凋亡细胞进行了观察,并用免疫组化方法同时对增殖细胞(PCNA染色阳性细胞)进行了分析。结果 在肾小球细胞增殖的同时,MPGN和LN患者表现出明显的凋亡不足的征象,而APGN患者则伴活跃的细胞凋亡。凋亡细胞数在APGN明显大于MPGN和LN,而增殖细胞与凋亡细胞的比值则以MPGN最大。结论 不同的增生性肾炎由于病因和病变性质不同,虽然都表现出细胞增殖,但其凋亡机制的反应性存在着明显的差异,这可能是上述三种增生性肾炎预后不同的因素之一。

 
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