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增殖细胞
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  proliferating cell
    A quantitative study of microvascular density and proliferating cell ratio in buccal mucosa squamous cell carcinoma
    颊粘膜鳞癌微血管密度与增殖细胞比率的定量研究
短句来源
    Quantitative Study of Microvascular Density and Proliferating Cell Ratio in Oral Squamous Cell Carcinoma
    口腔鳞癌血管密度与增殖细胞比率关系的研究
短句来源
    Immunohistochemical study was applied to explore clinicopathologic characteristic and behavior and expression of c-erbB-2 oncogene , proliferating cell nuclear antigen(PCNA) and mutant p53 protein in malignant myoepithelioma(MME) of salivary glands .
    本研究采用免疫组化技术探讨癌基因c-erbB-2、增殖细胞抗原(PCNA)与抑癌基因p53在涎腺恶性肌上皮瘤(MME)中的表达与临床病理的特点。
短句来源
    Therefore, we think that we may further observe the change of proliferating cell fraction with Ki-67 positive fraction of leukemic cell by ABC immunostaning method and use it as an index for studying leukimia clinically.
    作者认为,用ABC方法检测白血病细胞Ki-67阳性率可以更进一步观察细胞周期中增殖细胞比例的变化,并作为白血病临床研究的一项指标。
短句来源
    To investigate correlation of proliferating cell nuclear antigen(PCNA) and C-myc protein expression in ovarian epithelial carcinoma, two monoclonal antibodies, PCNA PC,, and antic-myc protein were used in ummunohistochemical reaction to measure PCNA and C-myc protein in paraffin section of 32 ovarian epithelial carcinoma, 10 ovarian epithelial tumor and 6 normal controls, and clinical pathological sechons of ovarian epithelial carcinoma were also examined.
    为探讨增殖细胞抗原(PCNA)及C-myc蛋白在卵巢上皮癌中的表达及临床意义,应用SABC免疫组织化学方法,对32例卵巢上皮癌、10例卵巢上皮瘤和6例正常卵巢组织的石蜡标本作PCNA和C-myc蛋白的检测,并分析卵巢上皮癌的临床病理资料。
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  proliferative cell
    A study on the labeling of melanocytic tumors and nevi with anti-human proliferative cell antibody Ki-67
    黑素细胞肿瘤抗人增殖细胞抗体Ki-67标染研究
短句来源
    RELATIONSHIP BETWEEN INFILTRATION OR METASTASS AND PROLIFERATIVE FEATURES OF SUPERFICIAL SPREADING TYPE OF HUMAN STOMACH CANCER. Ⅱ. STUDY OF PROLIFERATIVE CELL IN HUMAN STOMACH LABELED BY BRDU
    浅表扩散型胃癌的增殖特征与扩散转移的关系——胃癌增殖细胞Brdu体外标记研究之Ⅱ
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    The differences in the HCG labeling index and proliferative cell index were statistically significant ( P <0 01) between PSTT, EPS and choriocarcinoma.
    PSTT的HCG和增殖细胞指数与EPS和绒毛膜癌相比差异均有显著性 (P <0 0 1)。
短句来源
    Objectives: To explore the relationship between proliferative cell and histopathologic style and the depth of invasion of bowel wall in colonic carcinoma.
    目的 :探讨结肠癌增殖细胞与组织学类型、肠壁浸润深度间的关系。
短句来源
    At the 49 h point, apoptosis increased significantly in the proliferative cell zone in both carcinoma and the normal tissues.
    在49 h组,无论是正常组织还是癌组织的增殖细胞区内的凋亡都显著增多。
短句来源
  proliferation cell
    AIM: To study the influence on the expression of proliferation cell nuclear antigen(PCNA) in rectal carcinoma through retention -enema with FUDR and 5 -FU.
    目的:比较氟脲嘧啶脱氧核苷(FUDR)、5-氟尿密啶(5-FU)保留灌肠对直肠癌增殖细胞抗原(PCNA)表达的影响。
短句来源
    Objective To study the Influence on the expression of proliferation cell nuclear antigen (PCNA)in rectal carcinoma through retention-enema with L-OHP and 5-FU.
    目的比较奥沙利铂(L-OHP)和5-氟尿嘧啶(5-FU)手术前保留灌肠对直肠癌增殖细胞抗原(PCNA)表达的影响,为直肠癌的灌肠化疗提供理论依据。
短句来源
    The intratumor microvessel density and the proliferation cell lable inthe tissues with positive bFGF staining were significantly higher than those with negitive staining.
    并且表达组肿瘤微血管密度及增殖细胞指数均高于未表达组(P<0.05),提示bFGF在膀胱移行细胞癌中有促进微血管形成及肿瘤细胞增殖的作用。
短句来源
  “增殖细胞”译为未确定词的双语例句
    Significance of the S phase fraction analysis in evaluation of prognosis of esophageal squamous cell cancer
    食管鳞状细胞癌细胞核S期增殖细胞比率分析在评价预后中的意义
短句来源
    The clinical significance of DNA ploidy S-phase fraction and proliferation index determination in nephroblastoma
    肾母细胞瘤组织中DNA倍体、SPF、增殖细胞指数的测定
短句来源
    Ki-67 is a kind of cellular nuclear antigen and exist in G1 ana-phase and S,G2,M phase, but not in quiescent G0 phase.
    Ki67是一种与细胞密切相关的核抗原,存在于细胞周期的G_1后期、S、G_2、M期,而不存在于静止的G_0箱期细胞,是应用广泛的增殖细胞标记之一,被用以估计肿瘤细胞的增殖能力。
短句来源
    Purpose:To detect the expression of metallothionein in rectal adenocarcinoma and in their corresponding incisal edge normal tissue,and to study the correlation of MT with their clinicalpathological features, bcl-2 as well as PCNA in rectal adenocarcinoma.
    [目的]:检测金属硫蛋白(metallothionein-MT)在直肠腺癌与切缘“正常”组织中的表达。 探讨MT在直肠腺癌中与临床病理学、增殖细胞核心抗原(proliferation cell nuclear antigen-PCNA)、抗凋亡蛋bcl-2的相关性,分析直肠腺癌组织中MT与增殖、凋亡的关系。
短句来源
    Conclusion Interventional chemotherapy can induce apoptosis of human cervical cancer cells and inhibit the proliferation .
    2.正常宫颈组织、宫颈癌介入前、后组织中均有增殖细胞。 宫颈癌组织介入化疗后, LI较介入化疗前明显降低,说明介入化疗能抑制宫颈癌细胞的增殖。
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  proliferating cell
Special attention is given to the regulation of excision repair by different proteins-proliferating cell nuclear antigen (PCNA), p53, and proteasome.
      
Synthesis with eukaryotic DNA polymerases α, δ, and ε involves various replication factors, including the replication protein A, replication factor C, proliferating cell nuclear antigen, etc.
      
This is done by forming complexes of polymerase δ or ? with proliferating cell nuclear antigen (PCNA) and replication factors RP-A and RF-C.
      
The endothelium proliferation was evaluated using antibodies to proliferating cell nuclear antigen.
      
The obtained data indicate an important role of cardiomyocyte polyploidy and of activation of the proliferating cell nuclear antigen in development of myocardial hypertrophy in patients with OHCMP.
      
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  proliferative cell
A decrease of MN frequency in older groups could be explained by a gradual decrease of proliferative cell capacities.
      
Taking this view, any proliferative cell in the liver can be susceptible to neoplastic transformation.
      
Immunohistochemical assessment of cytokeratins 14 and 10/13, involucrin, and proliferative cell nuclear antigen (PCNA) demonstrated that keratinocytes differentiated in a manner similar to keratinocytes in normal epidermis.
      
Coexpression of TERT with proliferative cell nuclear antigen (PCNA) immunoreactivity is found in rod progenitor cells.
      
The toxic effect of cyclophosphamide on the proliferative cell population of hair follicles plucked from the human scalp was examined by the in vivo nuclear aberration assay.
      
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  proliferation cell
Immunohistochemical investigations included expression of proliferation markers (proliferation cell nuclear antigen and MIB-1) along with cell surface markers reflecting the cell-to-cell interaction (i.e.
      
Expression of proliferation cell nuclear antigen (PCNA; 30.6±11.7% in CN and 13.4±7.3% in non-caterpillar myocyte nuclei; P=0.0115) and cyclin B1 (2.8±3.8% and 12.6±15.6%, respectively; P=n.s.) was also positive in these nuclei.
      
In addition, the localization of proliferation cell nuclear antigen (PCNA) and basic fibroblast growth factor (b-FGF) was demonstrated immunohistochemically.
      
At PN2, the width of the PDL-forming region showed a minimum, but with a higher expression of NOGGIN and proliferation cell nuclear antigen than the other regions.
      
Preadipocyte proliferation (cell number, 3H-thymidine incorporation into DNA) decreased as the level of CLA increased in the cultures.
      
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Biochemical & Immunological Diagnosis Section, Shanghai Cancer Institute, Shanghai The method described involves two successive reactions: a phosphorylation of the substrate glucose by ATP under the action of hexokinase(HK) and determination of the remainder glucose with a commercial preparation(RDSG) which is a lyophylized mixture of glucose-oxidase, peroxidase and colouration dye. The method is sensitive specific and has added advantages of the technique being simple and of the reagents being easily obtained....

Biochemical & Immunological Diagnosis Section, Shanghai Cancer Institute, Shanghai The method described involves two successive reactions: a phosphorylation of the substrate glucose by ATP under the action of hexokinase(HK) and determination of the remainder glucose with a commercial preparation(RDSG) which is a lyophylized mixture of glucose-oxidase, peroxidase and colouration dye. The method is sensitive specific and has added advantages of the technique being simple and of the reagents being easily obtained. The average HK activity in 17 normal healthy mice is found to be 2.15 IU(0.65 to 3.65 IU) and in 28 AHC mice 16.20 IU(11.20 to 21.19 IU).Serial periodic determinations of the-HK activity during AHC cell proliferation showed that the enzymatic kinetic levels correlate closely with fluctuation of the cell counts and that this enzyme may be a useful biochemical marker of the disease.

本文介绍一种测定小鼠血浆中己糖激酶活力的光电比色法。其优点为材料易得、操作简便、敏感和特异。利用本法测得17只正常小鼠血浆HK活力为2.15±1.50IU,28只腹水型肝癌小鼠HK活力为16.20±4.99IU。两组的HK活力之间差异极其显著。在癌细胞增殖过程中,HK水平的动态变化曲线与增殖细胞数目-时间曲线十分一致;提示HK是癌细胞增殖过程的一个有用的生化标志。对本法及上述结果的潜在应用也进行了讨论。

3HTdR-autoradiography and Feulgen reaction were carried out successively in smear preparations of single cells. The proportion of proliferating cells ( S, G2 ) labeled by silver grains was counted, then Feulgen-density of unlabeled cells (G1 ) in the same slide was determined cytophotometrically. Although the labeling index of the active lymphocytes induced by PHA in vitro reaches 30.17%, isotope does not seem to be incorporated into the cells of juvenile polyps, and only small number of cells in various types...

3HTdR-autoradiography and Feulgen reaction were carried out successively in smear preparations of single cells. The proportion of proliferating cells ( S, G2 ) labeled by silver grains was counted, then Feulgen-density of unlabeled cells (G1 ) in the same slide was determined cytophotometrically. Although the labeling index of the active lymphocytes induced by PHA in vitro reaches 30.17%, isotope does not seem to be incorporated into the cells of juvenile polyps, and only small number of cells in various types of adenomatous polyps and adenomas were labeled by silver grains. The DNA content ( 24.06 ) of lymphocytes in prometaphase ( 4C ) is used as a reference standard, DNA indices ( DI ) were calculated from the ratio of the DNA content in the analyzed cells to normal diploid value( 12.03). The cellular DNA content in 5 cases of juvenile polyps is mainly within normal diploid range ( mean 12.06, DI=1.00) , however, hyperdiploid abnormality prevails in the 5 cases of abnomatous polyps and adenomas(mean 14.36, DI=1.19). The difference of the DNA value between two groups of polyps is statistically significant. Because the former group has no apparent: relation to adenocarcinoma, whereas the latter group frequently tends to undergo malignant change, these data led us to speculate that abnormal DMA content or aneuploidy may be considered as a certain sign of malignancy.

将单个细胞涂片连续进行~3HTdR-放射自显术和福尔根反应,先计算银粒标记的增殖细胞(S,G_2)的比例,再用细胞光度法测量未标记细胞(GI)的福尔根光密度。虽然受PHA刺激的活性淋巴细胞的标记指数达30.17%,但幼年性息肉不能参入同位素,各种腺瘤性息肉和腺瘤中只有少数标记细胞。以前中期(4C)淋巴细胞的相对DNA含量(24.06±2.67)为衡量标准,在与二倍体值(2C,12.03)比较后算出测量细胞的DI。5例幼年性息肉细胞的DNA含量为二倍体值(平均12.06,DT=1.00),5例腺瘤性息肉和腺瘤为超二倍体值(平均14.36,DI=1.19)。两类息肉的DNA含量差异显著。鉴于前者与腺癌无关,后者易于癌变。因此,不正常DNA含量或非整倍体性可能是细胞癌变的征兆。

Human brain glioma cells (SHG-44 cell line) were cultured in static liquid-overlayer conditions as multicellular spheroids. This method form spheroids homogeneous in size and almost perfect spherical in morphology and is quick (usually within 3 to 4 days), simple, economic and reproducibe. When individual spheroids with diameter about 200 μm were transferred to wells, their diameters increased 10 to 15 μ m/day and the doubling times were 6.0± 1.7 days, similar to the 7 days of the transplants in nude mice and...

Human brain glioma cells (SHG-44 cell line) were cultured in static liquid-overlayer conditions as multicellular spheroids. This method form spheroids homogeneous in size and almost perfect spherical in morphology and is quick (usually within 3 to 4 days), simple, economic and reproducibe. When individual spheroids with diameter about 200 μm were transferred to wells, their diameters increased 10 to 15 μ m/day and the doubling times were 6.0± 1.7 days, similar to the 7 days of the transplants in nude mice and different from the 2.6 days of; the monolayer cultures. Histology and 3H-TdR autoradiography revealed that cells in spheroids more than 300 μm were in three layers: a rapidly proliferating outer zone; an intermediate zone containing few generating cells and an inner zone containing pyknotic and necrosis cells. The thickness of viable cell leyers were 75-150 μm, comprising of 10 to 25 cell layers, similar to the oxygen and nutrient diffusion distance of 100- 150 μm in nodular carcinomas. The. percentages of cells in G1/G0. S and G2/M phase with monolayer cultures, spheroids and transplants were 36.7%, 58.6%, 4.7%, 52.9%, 35.7%, 11.4% and 56.2%, 27.1%, 16.7% respectively. The cells in S Phase were dominant in the monolayer cultures; but the fractions of G1/G0 cells were dominant in the spheroids which is similar to those of transplants and many human solid tumors in vivo.

本文报道人脑胶质瘤 细胞系SHG-44多细胞球体的制奋过程,并对其生物学特性进行初步探讨,为球体作为体外实体瘤 模型用于各种研究提供科学依据。用静止培养法所得到的球体大小均匀,形态较圆和规则,一般3-4天即可形成完整紧密的球体。球体的生长速率为10-15μm/天,倍增时间为6.0±1.7天,与单层细胞的2.5天相差较大,而与裸鼠体内移植瘤的7天相接近。组织学和~3H-TdR自显影结果表明,直径大于300μm的球体可分为三层:外层主要为增殖细胞;中层主要为静止细胞和少量增殖细胞;内层主要为核固缩和核破碎细胞。球体外周可见由10-25个细胞排列而成的75-150μm厚的活细胞层,与文献报道的结节性肿瘤中毛细血管的有效扩散距离100-150μm相接近。在单层细胞、珠体及裸鼠体内移植瘤中,G_1/G_O,S,G_2/M期细胞所占的比例分别为36.7%,58.6%,4.7%;52.9%,35.7%,11.4%及56.2%,27.1%,16.7%,单层细胞以S期细胞为主,而球体以G_1/G_O期细胞占优势,类似体内实体瘤。

 
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