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小麦原生质体
相关语句
  wheat protoplasts
     Study on Some Factors in Wheat Protoplasts Culture
     影响小麦原生质体培养因素的研究
短句来源
     TRANSFORMATION OF WHEAT PROTOPLASTS BY BYDV CP GENE AND THE REGENERATION OF TRANSGENIC PLANTS
     BYDV CP基因转化小麦原生质体及转基因植株再生
短句来源
     The re-sults showed that over 90% cells alinged in chains in alternating field,and that the per-centage of fused wheat protoplasts was over 90.Viability test (FDA) indicated that 80%~90% of the fused wheat protoplats induced by field strength 2 000V/cm,duration40μs,3 time pulses were alive.
     在融合脉冲作用下,小麦原生质体融合率最高可达90%。 用荧光双醋酸脂(FDA)测定活力,结果表明:两种小麦原生质体在2000V/cm 脉冲场强作用下,诱导融合后,成活率达80%~90%。
短句来源
     Gene Transformation of Wheat Protoplasts by Electroporation
     小麦原生质体的电激介导基因转移
短句来源
     ISOLATION, FUSION OF SOYBEAN AND WHEAT PROTOPLASTS AND SCREENING OF FUSANTS
     大豆和小麦原生质体的分离、融合及融合体的筛选技术
短句来源
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  protoplasts of wheat
     THE STABLE TRANSFORMATION OF THE COAT PROTEIN(CP) GENE OF BARLEY YELLOW DWARF VIRUS(BYDV) IN PROTOPLASTS OF WHEAT
     大麦黄矮病毒(BYDV)外壳蛋白基因在小麦原生质体中的稳定转化
短句来源
  wheat protoplast
     The changes in contents of DNA, RNA and soluble protein and the SDS-PAGE of soluble protein during somatic embryogenesis in wheat protoplast culture were studied.
     研究了小麦原生质体培养形成的体细胞胚胎发生过程中DNA、RNA和可溶性蛋白质含量的变化,并对可溶性蛋白质进行了SDS-PAGE分析。
短句来源
     Enhanced Wheat Protoplast Isolation Through Negative Pressure Treatment
     利用负压处理提高小麦原生质体解离效率
短句来源
  “小麦原生质体”译为未确定词的双语例句
     Study on Influencing Factors in Protoplast Culture of Wheat
     小麦原生质体培养影响因素的研究
     Isolation of Protoplasts and Chloroplasts from Wheat Leaf and Measurement of Their Photosynthetic Rate
     小麦原生质体和叶绿体分离及其光合速率的测定
短句来源
     WHEAT(TRITICUM AESTIVUM L.),CV.CHINESE SPRING,PROTOPLAST CULTURE AND MORPHOGENESIS
     “中国春”小麦原生质体培养与形态分化
短句来源
     PROTOPLAST CULTURE AND WHEAT PLANTLET REGENERATION(TRITICUM AESTIVUML.)
     小麦原生质体培养与植株再生的研究
短句来源
     ELECTRO ENHANCEMENT OF THE FORMATION OF CELL CLUSTERS FROM PROTOPLASTS OF TRITIUM AESTIVUM
     微直流对小麦原生质体形成细胞团的促进作用
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  wheat protoplasts
Both two-dimensional and three-dimensional imaging showed that the plasmalemma surfaces of winter wheat protoplasts were covered with numerous protruding particles.
      
The promoter activity measured by transient assays in wheat protoplasts was similar among the three homoeologs of TaBx3 and TaBx4 in spite of their differential transcript levels in wheat seedlings.
      
Wheat protoplasts were metabolically inactivated by iodoacetamide before fusion, while protoplasts of Italian ryegrass were X-ray irradiated before protoplast isolation.
      
Isolation, culture and morphogenesis from wheat protoplasts, and study of expression of DNA constructs by direct gene transfer
      
  protoplasts of wheat
Intergeneric somatic hybridization was performed between albino maize (Zea mays L.) protoplasts and mesophyll protoplasts of wheat (Triticum aestivum L.) by polyethylene glycol (PEG) treatments.
      
Plant regeneration from protoplasts of wheat (Triticum aestivum cv.
      
Culture of and fertile plant regeneration from regenerable embryogenic suspension cell-derived protoplasts of wheat (Triticum ae
      
Two types of protoplasts of wheat (Triticum aestivum L.
      
To study the effect of γ-ray treatment on donor and derived somatic hybrids, we carried out γ-ray donor treatment experiments with a wide range of γ-ray dosages and asymmetric somatic hybridization between protoplasts of wheat (Triticum aestivum L.
      
  wheat protoplast
Wheat protoplast culture: embryogenic colony formation from protoplasts
      
Seventeen and 25 putative hybrid clones were produced from the fusion combinations I and II, each with the donor wheat protoplast treated by UV light for 30?s and 1?min, respectively.
      
Whole plants obtained from Nakasoushu protoplasts were fertile, demonstrating the first report of Japanese cultivars in wheat protoplast cultures.
      


Freezing tolerance of isolated protoplasts of three wheat varieties which differ from each other in cold resistance has been measured.The results obtained in the present study indicate that the survival percentage of wheat protoplasts after freez- ing treatment is in-direct correlation with the cold resistance of its varieties.It is discussed that the change in plasmolemma plays a role in the mechanism of chilling injury and cold resistance of plants, and the results obtained may be used as an in- dicator for...

Freezing tolerance of isolated protoplasts of three wheat varieties which differ from each other in cold resistance has been measured.The results obtained in the present study indicate that the survival percentage of wheat protoplasts after freez- ing treatment is in-direct correlation with the cold resistance of its varieties.It is discussed that the change in plasmolemma plays a role in the mechanism of chilling injury and cold resistance of plants, and the results obtained may be used as an in- dicator for determining the cold resistance of the plants.

对三个不同抗寒力小麦品种的分离原生质体进行了耐冻性的测定,结果指出,小麦原生质体在冰冻处理后的存活率与其品种的抗寒力成正相关。讨论了质膜的变化在寒害和抗寒机制上的作用,以及本结果作为鉴别植物抗寒力指标的意义。

Procedures have been developed for the isolation of wheat leaf protoplasts and soybean root tip meristem protoplasts. Fusion between protoplasts has been achieved using polyethylene glycol. The fusion products has been selected by micropipette.

本文提供了大豆(Glycine max)根尖分生组织细胞原生质体和小麦叶栅栏细胞原生质体的分离方法。建立了大豆和小麦原生质体融合的实验程序和融合体筛选技术。

The principls and circuit of electrofusion apparatus,model CY-1,are introduced inthis paper.The apparatus is composed of integrate circuits.The animal cells or plantprotoplasts could be aligned in chains in the alternating field (5kHz~2MHz,0~250V/cm) between the electrods of fusion cell.The direct current pulses (duration 10~150μs, 0~300V) could cause reversible membrane breakdown,then the contacting cellsor protoplats fused.Experiments on electrofusions between wheat protoplasts of cell sus-pension and mesophell...

The principls and circuit of electrofusion apparatus,model CY-1,are introduced inthis paper.The apparatus is composed of integrate circuits.The animal cells or plantprotoplasts could be aligned in chains in the alternating field (5kHz~2MHz,0~250V/cm) between the electrods of fusion cell.The direct current pulses (duration 10~150μs, 0~300V) could cause reversible membrane breakdown,then the contacting cellsor protoplats fused.Experiments on electrofusions between wheat protoplasts of cell sus-pension and mesophell cells,microbe protoplasts (Gibberella fujikuroi Wr.),or animalcells (myeloma and spleen cells of mouse) have been done using this apparatus.The re-sults showed that over 90% cells alinged in chains in alternating field,and that the per-centage of fused wheat protoplasts was over 90.Viability test (FDA) indicated that 80%~90% of the fused wheat protoplats induced by field strength 2 000V/cm,duration40μs,3 time pulses were alive.

南农CY-1型细胞融合仪的主线路全部由集成电路组成。它能在电融合小室的电极间产生一个交变电场(5kHz~2MHz,0~250V/cm),使动植物细胞排列成串,其输出的直流融合脉冲(脉宽10~150μs,0~300V)能成功地诱导相邻原生质体局部接触区质膜的可逆击穿而形成融合。用该仪器对小麦(T.aestivum)悬浮培养系和叶肉原生质体、微生物原生质体(水稻恶苗病菌)和动物细胞(小鼠骨髓瘤细胞与脾细胞)进行了融合试验.结果表明,在交变电场中,各种细胞成串率均达90%以上;在融合脉冲作用下,小麦原生质体融合率最高可达90%。用荧光双醋酸脂(FDA)测定活力,结果表明:两种小麦原生质体在2000V/cm 脉冲场强作用下,诱导融合后,成活率达80%~90%。

 
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