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dna特征
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  dna character
     Objective:To compare the DNA character with in cholesteatoma tissues with that in normal skin.
     目的 :采用流式细胞术分析胆脂瘤上皮细胞的 DNA特征 ,探讨胆脂瘤的病理学属性。
短句来源
     Agarose gel electrophoresis was used to analyze DNA character and the flow cytometry to detect apoptotic rate and cell cycle distribution.
     琼脂糖凝胶电泳分析DNA特征; 流式细胞仪检测细胞凋亡和细胞周期分布;
短句来源
  dna characteristic
     Study on the components of cellular fatty acids and DNA characteristic of Escherichia coli. O157∶H7
     大肠埃希菌O157∶H7脂肪酸组分及DNA特征研究
短句来源
  dna characteristics
     Here we reported the results of the distributions of the virulence genes (ctx, zot, ace, RS1) among 471 isolates of VC O1 by colony hybridization, and the genomic DNA characteristics of 34 isolates of VC O1 by PFGE and Southern blot with ctx probe.
     应用原位杂交法对471株O1群VC的主要毒力基因分布情况进行检测,用核酸脉冲场凝胶电泳对其中34株进行染色体DNA特征和CT基因Southern杂交分析。
短句来源
     Aim To study DNA characteristics and pathogenic expression of Yersinis enterocolitica.
     目的 探讨致病性小肠结肠耶氏菌 (简称 :耶氏菌 )DNA特征及其表达。
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  “dna特征”译为未确定词的双语例句
     Results 19 specific CpG motifs were ascertained and 504 12-ODNs were detected in Adv2 DNA and Adv5 DNA.
     结果确定了19种Adv2、5DNA特征性的CpG基序,Adv2、5DNA中以这19种CpG基序为核心的12-ODN共504条。
短句来源
     Methods Sequences of Adv2, 5, 12 DNA and EC DNA were obtained from the Entrez Nucleotides database at NCBI. The specific CpG motifs of Adv2 DNA and Adv5 DNA were identified after above sequences were analyzed and compared by softwares such as DNATools, BioEdit, and so on. All the 12-ODNs with specific CpG motif core were searched from Adv2 DNA and Adv5 DNA.
     方法从NCBI核酸数据库中获取Adv2、5、12DNA和ECDNA序列,采用DNATools、BioEdit等软件对Adv2、5、12DNA和ECDNA进行序列分析和比较,确定Adv2、5DNA特征性的CpG基序,查找出Adv2、5DNA中以上述CpG基序为核心的12碱基寡核苷酸(12-ODN)。
短句来源
     Study on the characteristics of randomly amplified polymorphic DNA of Vibrio Cholera non-O1 O_(139) and O1 serogroup
     O_(139)霍乱弧菌随机扩增多态性DNA特征
短句来源
     DNA fragmentation was visual- ized by agarose gel electrophoresis; cell cycle arrest was detected by flow cytometry. The p21 and cdk4 mRNA expression of HepG2 was detected by RT-PCR.
     方法:培养HepG2,应用四氮唑蓝(MTT)比色法观察FK228对HepG2的生长抑制作用,琼脂糖凝胶电泳分析细胞DNA特征,流式细胞术分析细胞周期,以RT-PCR检测HepG2细胞中p 21,cdk4基因表达水平。
短句来源
     Molecular probe method is based on DNA sequences of phytoplanktons, which is at the stage of laboratory research.
     分子探针法基于浮游植物的DNA特征,处于实验室研究阶段。
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      dna characteristic
    The increased proportion of 5S DNA characteristic of the dnaB extract and the lack of Okazaki piece synthesis characteristic of the dnaG extract were both apparent in analysis of the dnaB dnaG mutant extract reaction.
          
    In these experiments, there is hybridization associated with the broad distribution of DNA characteristic of that species.
          
      dna characteristics
    The strains in each species were indistinguishable with respect to phenotypic features and general DNA characteristics as determined by restriction analysis.
          
    When detailed knowledge of the ancestry of a natural population is lacking, the DNA characteristics of a sample of current generation individuals often provide a wealth of information in this respect.
          
    When vinblastine-free medium was used for culturing, the changed DNA characteristics returned to their original values.
          
    Interspecific transformation and DNA characteristics in Allomyces
          
    All of these known species are closely related on the basis of DNA characteristics such as base composition and thermal denaturation profiles of major DNA components.
          
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    5-methylcytosine (m5C) as a rare base exists in eucaryotic genomes, it is a normal constituent of many eucaryotic DNA, whose existence is a character of eucaryotic DNA. In the regular physiological conditions, cytosine residue of eucaryotic DNA is methylated to be popular. Up to the present, many people consider that the m5C may be mutation hotspots by the m5C deamination leading to gene mutation. Our theoretical investigations indicated that the spontaneous mutation caused by the transition of G - C-A - T,...

    5-methylcytosine (m5C) as a rare base exists in eucaryotic genomes, it is a normal constituent of many eucaryotic DNA, whose existence is a character of eucaryotic DNA. In the regular physiological conditions, cytosine residue of eucaryotic DNA is methylated to be popular. Up to the present, many people consider that the m5C may be mutation hotspots by the m5C deamination leading to gene mutation. Our theoretical investigations indicated that the spontaneous mutation caused by the transition of G - C-A - T, in eukaryotic DNA, may be a result caused by the tautomer changing base pairs and may also be caused by other factor actions, however it could not be caused by the deamination of m5C.

    迄今为止,不少人认为5-甲基胞嘧啶的存在可能是基因突变的“热点”。然而,事实上5-甲基胞嘧啶作为一种稀有碱基,本来就是许多真核生物DNA的正常组分。它的存在也是真核生物DNA的特征之一。 我们几年来所作的理论研究表明,5-甲基胞嘧啶不可能是基因突变的“热点”。由胞嘧啶甲基化成为5-甲基胞嘧啶,再经水解脱氨成为胸腺嘧啶的反应(C→m~5C→T)在正常生理条件下是不太可能发生的。在真核生物DNA中,G—C碱基对转变为A—T碱基对的反应可能是由互变异构碱基配对或其它因素引起的。

    he plasmid DNA content of 828 Y. pestis strains isolated from Yunnan province were detected by agrosegel electrophoresis method. The result showed that these strains carried nine different kinds of plasmid withthe molecular weight about 3.93,6.05,22.97,35.65,45.35,64.82,74.59,111. 36 and 129. 55 Mdal,and ac-cording to the plasmid constituent of these strains,the plasmid profile can be divided into ten types. Amongthese strains, 99.15%strains have three normative plasmids with molecular weight about 6.05, 45.35,and64.82...

    he plasmid DNA content of 828 Y. pestis strains isolated from Yunnan province were detected by agrosegel electrophoresis method. The result showed that these strains carried nine different kinds of plasmid withthe molecular weight about 3.93,6.05,22.97,35.65,45.35,64.82,74.59,111. 36 and 129. 55 Mdal,and ac-cording to the plasmid constituent of these strains,the plasmid profile can be divided into ten types. Amongthese strains, 99.15%strains have three normative plasmids with molecular weight about 6.05, 45.35,and64.82 Mdal,and 21.62%,4.59%,1.45%and 3.5%strains of the total existed 3.93, 22.97, 35.65 and111.36 Mdal plasmid respectively besides three normative plasmids, Those strains with non-normative plas-mids have a special region of distribution and it has an important moleculo─epidemiological significance.On thebasis of plasmid profile, our provincial pestilence district may be divided into seven relatively independentplague foci,namely,the northwest Yunnan mountain area,the Baoshan Basin,the Yingjiang river valley, theLongchuang river valley,the Nanding river valley,the Lancang river(Iower reaches) vaIley and the Yuanjiangsection of Honghe river.This study showed that plasmid content may be used as an important criterion in the division of middle plague foci in Yunnan plague foci. The Y.pestis strains isolated from domestic rat type and wild rodent type plague foci in Yunnan have a common character of heredity and mutation. In recent years,the epidemics may be due to resurgence but it may spread to other area also.There are natural plague foci existing in the sourth-weste Yunnan.

    用琼脂糖凝胶电泳技术,检查了分离自云南省的828株鼠疫菌质粒DNA特征。结果表明,云南鼠疫菌可观察到分子量为3.93、6.05、22.97、35.65、45.35、64.82、74.59、111.36和129.55Mdal九种质粒,按质粒组成可划分为Ⅰ~Ⅹ种质粒图谱。被检菌株中,99.15%具有6.05、45.35和64.82Mdal三种规范化质粒,还发现21.62%、4.59%、1.45%和3.5%的菌株分别尚存3.93、22.97、35.65和111.36Mdal质粒,这些质粒有特定的分布区域,具有重要的分子流行病学意义。根据质粒图谱,可将我省鼠疫现有疫区初步划分成滇西北山地、保山盆地、大盈江、陇川江、南定河、澜沧江下游和红河元江段流域七大相对独立的疫源区。本研究的结果结合既往流行病学资料,似可说明:①质粒组成可作为云南省鼠疫疫源地中疫源地划分的重要指标;②云南家、野鼠两型疫源地菌株具有共同的遗传变异性;③近几年之间鼠疫有复燃也存在异地传播;④云南西南部存在鼠疫疫源。

    Abstract This paper presented that twenty three viral specific cloned fragments were mapped after EcoRⅠand EcoRⅠ/Hind Ⅱ fragment libriary was established.Typical pox vinous purified by sucrose densitygradient centrifugation were observed under electron microscope after PTA-negative staining samples prepaired from two opalescent bands in the gradient column,but no sisnificant difference was detected between the preparation from the above banded samples which may be corelated with the two forms of fowlpox virus,the...

    Abstract This paper presented that twenty three viral specific cloned fragments were mapped after EcoRⅠand EcoRⅠ/Hind Ⅱ fragment libriary was established.Typical pox vinous purified by sucrose densitygradient centrifugation were observed under electron microscope after PTA-negative staining samples prepaired from two opalescent bands in the gradient column,but no sisnificant difference was detected between the preparation from the above banded samples which may be corelated with the two forms of fowlpox virus,the intracellular and extracellular form.The genomical DNA Shared typical feature of agsrose electrophretic behaviour of the high-molecule-weight.DNA were restricted with EcoRⅠor EcoRⅠ/Hind Ⅲ followed by ligation with the linerized pUC19 plasmid then transforming the E.colt.JM109 strain competent cells.Restficted fragment clone libriary was established after screening out the recombinants by colony color selection and AGE size fraction. Viral specific clones were further identified by in siu hybridization and dot blotting with Digeoxnin-labelled CEF DNA or FPV DNA probe.TOtally twenty three recombinant plasmids were mapped with the restriction endonucleases within the polylinker and no cleavable sites in pUC19 according to the double-enzyme-digotion method.Available insertion sit6s for further incorporation of the reporter gene cassette was located in each of the above cloned genomical fragments.This will be greatly facilitated the shotgun screening of the nonessential-region(NER)for virus replication as weil as the molecular virological study of PPV and its engineering as an expression vector.

    以鸡胚成纤维细胞培养的中国鸡痘病毒鹌鹑化弱毒株,经蔗糖密度梯度离心法纯化,电镜观察示所得病毒为典型鸡瘟病毒颗粒。以蛋白酶K法抽提病毒基因组,电泳结果为典型的大分子量DNA特征,经EcoRI或EcoRI/HindⅢ酶切,插入pUC19载体相应位点,经转化、筛选、鉴定后得相应酶切片段的基因组文库。对其中23个克隆片段用双酶法进行酶谱分析,绘制出了相应克隆片段的酶切图谱,每个克隆片段中均有1~6个可以利用的插入位点。基因组文库的构建及部分克隆片段的酶谱分析为进一步筛选病毒复制非必需片段以构建重组疫苗表达载体乃至鸡痘病毒分子生物学的研究打下了坚实的物质基础。

     
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