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| | The temperature of platelet intracellular ice crystal formation (IIF) is one of the most important physical parameters to instruct platelet cryopreservation. In this study, the range of temperatures for platelet IIF was measured by means of biological and physical methods. All platelet samples were graded cooling, and two samples of per 5℃ decrease were thawed by 2 different ways: 37℃ directly(T 37℃) and 37℃ after keeping in liquid nitrogen (LN) for 2 hours. The phosphatidylserine(PS) positive rate, plasma ... | | 血小板胞内冰晶形成 (IIF)的温度是指导血小板低温保存最重要的物理参数之一。本研究通过生物学和物理学的两种方法同时测定血小板IIF温度范围。在分步降温法中 ,每降 5℃取出血小板样本 ,测定 37℃直接复温(T37℃ )和经液氮处理后再 37℃复温 (LN)两种方法处理的血小板样本磷脂酰丝氨酸 (PS) ,血浆乳酸脱氢酶 (LDH)和血小板聚集功能的变化。同时利用差式扫描量热计 (DSC)记录加和不加 5 %DMSO的血小板在降温过程中的放热峰。结果表明 ,PS ,LDH和血小板聚集功能在 - 35℃左右不再发生变化。DSC测定结果表明 ,代表血小板IIF的第二个小放热峰在 - 35℃左右。结论 :血小板IIF温度在 - 30℃至 - 4 0℃之间 (- 35℃左右 )。 | | 文摘来源 | | In order to acquire freezing model of the cryopretective solution (NaCl propylene glycol water ternary system)for platelet, the melting points (T f) of this cryopretective solutions with different solute concentration and different ratio of PG mass to NaCl mass were measured by using a differential scanning calorimeter (DSC), and these experimental data were fitting by computer. An empirical equation was derived which characterized the Tf as a function of the solute concentration and the ratio of PG mass... | | 为了获得血小板冰冻保护液 (丙二醇 氯化钠 水 ,PG NaCl H2 O)冰冻降温的数学模型 ,本研究使用差示扫描量热仪分别测定出不同浓度和不同比例条件下丙二醇 氯化钠 水三元溶液的凝固点 (Tf) ,然后将已有的公式和实验所得的数据通过计算机进行拟合。结果获得了一个数学模型 ,该数学模型描述的是在PG NaCl H2 O溶液中Tf变化与溶质浓度和PG NaCl(质量比 )两个参数变化之间的函数关系。结论 :把该方程与血小板的渗透性特性的参数相结合 ,可以描述在平衡冷冻状态下 ,不同PG浓度时血小板的体积变化 ,指导血小板低温保存。 | | 文摘来源 | | Platelet clearance has already been studied in physiological and pathological conditions and shown its occurrence mainly in the liver and the spleen. It is still not clear what mechanisms are responsible for recognition and removal of either aged or damaged platelets by the scavenging system. So study of the clearance mechanism will be useful to prolong the survival time of plateletes in vivo. And it may be related to a new strategy to store platelets. This article focuses on the advances in studies of the ... | | 生理学,病理学方面的研究发现血小板在体内清除主要发生在肝脏和脾脏,但衰老和损伤的血小板被体内清除系统识别和清除的机制还并不清楚。这一机制研究的深入将有助于解决血小板低温保存后体内存活期短,不能较长期发挥作用的难题,为血小板保存提出新的策略。本文就血小板回输体内后的清除机制,包括P-选择蛋白,GPⅠbα及其受体Mac-1和内源性金属蛋白酶在血小板清除进程中的作用等的研究进展作一综述。 | | 文摘来源 | |   |
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