Objective: To investigate the genetic polymorphism of human platelet antigen(HPA)1 through 5 and 15 systems in Uighurs,which are closely related to refractoriness to platelet transfusion therapy.
Methods Anti GPⅡb/Ⅲa and anti GPⅠb/Ⅸ autoantibodies were detected by a modified monoclonal antibody immobilization of platelet antigens assay(modified MAIPA), and PAIgG by competitive ELISA.
Objective: To investigate the genetic polymorphism of human platelet antigen(HPA)1 through 5 and 15 systems in Uighurs,which are closely related to refractoriness to platelet transfusion therapy.
Method: Divide HPA_ 1-5 system accede of 72 patients in Zhanjiang by the technique of PCR-SSP. The inject matched-platelets with provided by the blood center of Zhanjiang for patients.
Methods Platelet bound autoantibodies in thrombocytopenic patients (immune and non immune) were measured by direct MAIPA. Monoclonal antibodies against GPⅡb/Ⅲa, GPⅠb and GPⅠa/Ⅱa were used.
Methods PAIgG was measured by using ELISA. Antibodies of and membrane protein of platelet (GPⅡb/Ⅲa, GPⅠb/Ⅸ, P-Selection) were measured by using MAIPA and MACE.
Most commonly, it is observed in PlA1-negative subjects previously sensitized with PlA1 platelet antigen either through PlA1-positive pregnancy or PlA1-positive transfusion.
Human platelet antigen (HPA) systems consist of more than eight biallelic antigen polymorphisms in which a base pair substitution leads to change in an amino acid of a glycoprotein expressed on the platelet.
In this study we used flow-cytometry to determine the influence of the modern interventional technique of rotablation on platelet antigens and their possible alteration.
Antiplatelet antibodies directed against glycoproteins Ib/IX and IIb/IIIa were determined by monoclonal antibody-specific immobilization of platelet antigens (MAIPA).
Human platelet antigen (HPA) systems consist of more than eight biallelic antigen polymorphisms in which a base pair substitution leads to change in an amino acid of a glycoprotein expressed on the platelet.
Human platelets were labelled with ~3H by the periodate method and with ~(125)I by the lactoperoxidase method. The antigens recognized by 14 monoclonal antibodies to human platelets(8 of which were identified as IgG) were charaterized by the SPA- immunoprecipitation technique. The monoclonal antibodies SZ-1, SZ-2 and SZ-3 reacted with a glycoprotein with MW 168, 000 under nonreduced conditions and with MW 142, 000 reduced, indicating the antigens of them were platelet glycoprotein Ib(GPIb). The monoclonal a...
latelet depletion abrogated platelet-activating factor (PAF) -and antigen-induced pulmonary resistance (RL) increase and dynamic compliance (Cdyn) decrease. The significant increase of chemiluminescence (CL) peak valuue couldbe obtained only by antigen challenging sensitized platelets. but not company 5-HT release.Sodium ferulate (SF.50and 100mg/kg iv) given 5min before antigen challenge Could dose-dependently inhibit antigen-induced airway immediate anaphylaxis.In Contrast, the inhibitory effect of SF on P...