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高频率再生
相关语句
  high frequency regeneration
     Study of Influence Factors of Rape High Frequency Regeneration
     油菜高频率再生影响因素的研究
短句来源
     Establishment of High Frequency Regeneration System for Tissue Culture of Alfalfa
     苜蓿组织培养高频率再生体系的建立
短句来源
     High Frequency Regeneration of Protoplasts from Litchi Transgenic Embryogenic Calli
     荔枝转基因胚性愈伤组织原生质体高频率再生
短句来源
     Therefore, in this paper, the recombinant plant expression vector pCAMBIA1300-hBMP-3m which had been constructed was introduces into Agrobacterium tumefaciens LBA4404. The system of high frequency regeneration and transformation of rape hypocotyls were set up.
     因此,本试验在已经构建好的植物表达载体pCAMBIA1300-hBMP-3m的基础上,通过电击法将其转入根癌农杆菌LBA4404中; 建立了油菜下胚轴的高频率再生体系和高频遗传转化系统;
短句来源
  high-frequency regeneration
     ESTABLISHMENT OF HIGH-FREQUENCY REGENERATION SYSTEM OF LEAF LETTUCE(Lactuca sativa var.Capitata L.)
     叶用莴苣高频率再生体系的建立
短句来源
     The callus induction and high-frequency regeneration of Dioscorea opposita Thunb
     怀山药微型块茎愈伤组织的诱导形成及高频率再生
短句来源
  “高频率再生”译为未确定词的双语例句
     Results of study on clone A87203 4C-1-5 from anther culture of homologous tetraploid rice were reported.
     同源四倍体水稻杂种花药离体培养,建立了一个高频率再生植株能力的花粉无性系A87203 4C-1-5。
短句来源
     HIGH—FREQUENCY PLANT REGENERATION FROM MONOCELL CULTURE OF ASTRAGALUS TENUIS
     细叶黄芪(Astragalus tenuis)单细胞培养高频率再生植株
短句来源
     The leaf regeneration and Agrobacterium tumefaciens-mediated transformation system of cherry rootstock 98-1,Colt and Dagingye were established at the first time.
     以优良樱桃砧木新品系 98 1、Colt、大青叶为试材进行叶片离体再生及根癌农杆菌介导遗传转化 ,建立了高频率再生系统 ,并获得抗菌肽转基因植株。
短句来源
     The high regeneration frequency were obtained when the proplasts were embedded in agarose and cultured in Km8P medium added glutamine and serine without NH4NO3 and KNO3.Shoot regeneration occurred when the protoplast-derived calli were transferred onto M+ BA 1mg/L differentiation medium.
     用琼脂糖包埋法,在不含NH4NO3及KNO3,而加有丝氨酸和谷氨酰胺的Km8P培养基上培养原生质体获得了高频率再生愈伤组织。
短句来源
     By this way, extremely shooty callus A87203, dwarf mutant H2558, monoculm mutant H2558, sterile mutant 890321, small panicle mutant H4198, grass-like dwarf mutant H5421, multi-tillers dwarf mutant H5597, yellow-leaf dwarf mutant 008318, wheat-type panicle mutant H3774, narrow-leaf dwarf mutant and non-palea mutant etc. were obtained. Most of them were controlled respectively by a pair of recessive gene, which would be excellent resources for gene isolation.
     现已获得高频率再生植株花粉无性系A87203、H2558矮变体、H2558独秆变异体、890321不育突变体、H4198小穗突变体、H5421草状突变体、H5597多蘖突变体、008318黄叶矮变体、H3774小麦型穗突变体H3774针状叶矮变体、H3774无内颖突变体等数十种,为功能基因组研究提供了丰富的材料。
短句来源
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  high frequency regeneration
High frequency regeneration of haploid plants through embryogenesis was achieved within 8 weeks.
      
Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro
      
High frequency regeneration occurred when somatic embryos were germinated on media that lacked 2,4-D.
      
Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants
      
High frequency regeneration and heritable somaclonal variation in Brassica juncea
      
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  high-frequency regeneration
A single-step, high-frequency regeneration pro-tocol has been standardised for Phytophthora-resistant wild pepper, Piper colubrinum (Link) using root, internode, leaf and petiole explants derived from in vitro plantlets.
      
The root: a potential new source of competent cells for high-frequency regeneration in Tylophora indica
      
Using a transverse thin cell layer culture system, we have identified some of the developmental and physiological constraints that limit high-frequency regeneration in sugarcane leaf tissue.
      
High-frequency regeneration of adventitious buds from callus was achieved when cultured on half-strength MS medium supplemented with 10% coconut water, 13.3 μM BA, and 2.5 μM zeatin.
      
High-frequency regeneration of shoots was achieved at root region of seedlings of Robinia pseudoacacia L.
      


Results of study on clone A87203 4C-1-5 from anther culture of homologous tetraploid rice were reported.The trials showed that the clone had a bud multiplication rate of 150—200 times, Under stereoscope,buds were differentiating on buds,forming bud masses.It had a callus induction frequency about 5 times when the bud masses were clipped into pieces and cultured on MS medium for dedifferentiation.Buds with the characteristics of high multiplication were able to be produced from about 50% of the calli when they...

Results of study on clone A87203 4C-1-5 from anther culture of homologous tetraploid rice were reported.The trials showed that the clone had a bud multiplication rate of 150—200 times, Under stereoscope,buds were differentiating on buds,forming bud masses.It had a callus induction frequency about 5 times when the bud masses were clipped into pieces and cultured on MS medium for dedifferentiation.Buds with the characteristics of high multiplication were able to be produced from about 50% of the calli when they were transferred onto MS me- dium for differentiation.It still maintained a vigorous multiplying ability though it had been subcultured for more than 30 generation during the past 1 year or more.Numerous plantlets were able to be produced by culture under regulated temperature and light conditions.A number of plantlets deriving from the buds had been planted in the fields this year.Most of them were normal,however,few plants had morphological variations,with 6 trisomic(2n+1 =25)plants observed during meiotic division of their pollen mother cells.

同源四倍体水稻杂种花药离体培养,建立了一个高频率再生植株能力的花粉无性系A87203 4C-1-5。试验结果表明:一个芽丛培养一个月,芽数增殖频率为150—200倍。芽的发生方式为芽上生芽。若将芽丛剪碎,重新接在 MS 诱导培养基上,愈伤组织重量的增殖率为5.0倍左右。再将愈伤组织转入 MS 分化培养基上,大约有50%愈伤组织块又可分化出芽,并且仍具有高频率再生芽的特性。继代培养30多代(一年多时间)后仍保持旺盛的增殖能力。通过控制光温条件,可迅速再生植株。植株移栽田间,大多数生长正常,少量外部形态变异。细胞学鉴定,6株为三体(2n+1=25)。

A satisfactory procedure for monocell culture of Astragalus teams is reported in this paper. By the procedure, the monocells of A. tenuis regenerate intact plants at a high frequency. Cell suspension lines are established from calli derived from stem segments of sterile seedlings of A. tennis. Monocells are isolated from the cell suspension lines and put into modified MS liquid media for vibration culture. First divisions of the monocells are observed after 2~3 days of culture, and a large number of microcalli...

A satisfactory procedure for monocell culture of Astragalus teams is reported in this paper. By the procedure, the monocells of A. tenuis regenerate intact plants at a high frequency. Cell suspension lines are established from calli derived from stem segments of sterile seedlings of A. tennis. Monocells are isolated from the cell suspension lines and put into modified MS liquid media for vibration culture. First divisions of the monocells are observed after 2~3 days of culture, and a large number of microcalli from monocells are produced after 35 days. A part of the microcalli are maintained in liquid media for suspension culture,and after two weeks,numerous young buds are differentiated on the calli surfaces. On transfer of the budding calli onto differentiation media,the young buds develop into shoots,and the shoots roote by transfer to rooting media. The other microcalli aTe transffered from liquid media to solid media for proliferation for two weeks,and then transffered them to differentiation media. On which, 100% of the calli produced shoots and 15~30 shoots could be produced per shooting callus. Effect of 2,4 —D on tissue and cell culture of A. tenuis are also discussed in the paper.

本研究建立了一套适合于细叶黄芪单细胞悬浮培养的良好系统.在该培养系统中,细叶黄芪单细胞高频率地再生了完整植株.由细叶黄芪种子无菌苗茎段诱导产生的愈伤组织建立了细胞悬浮系.从悬浮系分离的单细胞悬浮培养2~3天开始分裂,35天形成大量的小愈伤组织.小愈伤组织继续悬浮培养即可不断地分化出大量幼芽.愈伤组织在固体分化培养基上的分化率为100%.目前已获得了大量的再生植株.本文还就2,4—D对细叶黄芪和细胞培养的影响进行了讨论.

Two cultivars of sweet pepper, 'Zhongjiao 2' , and 'Shuangfeng' , were screened out of eight cultivars for their high capacity of regeneration. The shoot-bud differentiation frequency reached as high as 100% and each explant produced up to 11. 7 buds. Various factors influencing in vitro shoot-bud differentiation and the shoot elongation in particular, which was a major problem in pepper tissue culture, were investigated. Our experimental results showed that the seedling age was very important, i.e. the cotyledon...

Two cultivars of sweet pepper, 'Zhongjiao 2' , and 'Shuangfeng' , were screened out of eight cultivars for their high capacity of regeneration. The shoot-bud differentiation frequency reached as high as 100% and each explant produced up to 11. 7 buds. Various factors influencing in vitro shoot-bud differentiation and the shoot elongation in particular, which was a major problem in pepper tissue culture, were investigated. Our experimental results showed that the seedling age was very important, i.e. the cotyledon explants taken from 12-day-old seedlings displayed the highest shoot-bud regeneration capacity. As far as culture medium was concerned, the shoot-buds elongated well on MS+ZT2+GA_3 2mg/l. Addition of coconut milk or casein hydrolysate also promoted shoot elongation.

本研究从‘中椒2号’、‘双丰’、‘早丰52’、‘中椒3号’、‘中椒4号’、‘茄门’、‘海花29’、‘塞花 2号’等8个青椒(Capsicum annuum L.)基因型中筛选出‘中椒 2号’、‘双丰’两个对离体反应较好的基因型,建立了我国栽培品种的高频率再生体系,外植体分化频率可达100%,每外植体芽数可达11.7个。研究了各种因素对离体再生,特别是芽伸长的影响,发现12天苗龄的子叶分化效果最佳。在MS+ZT2+GA_32mg/l培养基上芽伸长效果较好,有机添加物椰乳(CM)、酪蛋白水解物(CH)也有促进芽伸长的作用。从培养起至形成小植株约2~3个月,与以往文献报道的7个月相比,成苗周期大大缩短。

 
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