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发酵生产工艺条件
相关语句
  fermentation process conditions
    Studies on the Optimization of Fermentation Process Conditions of the Ice Nucleation-active Bacteria Xanthomonas ampelinaTS206
    冰核活性细菌Xanthomonas ampelinaTS206实验室水平发酵生产工艺条件优化的研究
短句来源
    The fermentation process conditions of the ice nucleation-active bacteria Xanthomonas ampelina TS206 were studied in this paper. The results showed that the optimal culture temperature was 18℃, the volume of fermented broth 160ml/L, the optimal initial pH8.0 and the seed volume 3%.
    本文对冰核活性细菌XanthomonasampelinaTS206实验室水平的发酵生产工艺条件进行了系统的研究,结果表明这种冰核活性细菌的最适培养条件为:培养温度18℃,装液量40ml/250ml,摇床转速180r/min,接种量3%,发酵液的初始pH8。
短句来源
  fermentation process conditions
    Studies on the Optimization of Fermentation Process Conditions of the Ice Nucleation-active Bacteria Xanthomonas ampelinaTS206
    冰核活性细菌Xanthomonas ampelinaTS206实验室水平发酵生产工艺条件优化的研究
短句来源
    The fermentation process conditions of the ice nucleation-active bacteria Xanthomonas ampelina TS206 were studied in this paper. The results showed that the optimal culture temperature was 18℃, the volume of fermented broth 160ml/L, the optimal initial pH8.0 and the seed volume 3%.
    本文对冰核活性细菌XanthomonasampelinaTS206实验室水平的发酵生产工艺条件进行了系统的研究,结果表明这种冰核活性细菌的最适培养条件为:培养温度18℃,装液量40ml/250ml,摇床转速180r/min,接种量3%,发酵液的初始pH8。
短句来源
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  fermentation process conditions
Response-surface methodology was applied to determine the effect of the fermentation process conditions, namely pH, temperature, rates of agitation and aeration, on surfactin production.
      


After using a simple but efficient mini-bioreactor (2L) we equipped to study the effects of carbon source, nitrogensource and initial pH of fermentation broth on the production of microbial transglutaminase (MTG) by a new potent strain,Streptomyces sp. WZFF.L-M168 isolated from soil and preserved in our laboratories. The incubation conditions were established.The production was scaled up to 20 L and 200 L jar-fermenters through the utilization of on-line control techniques, and then thetechniques for batch fermentation...

After using a simple but efficient mini-bioreactor (2L) we equipped to study the effects of carbon source, nitrogensource and initial pH of fermentation broth on the production of microbial transglutaminase (MTG) by a new potent strain,Streptomyces sp. WZFF.L-M168 isolated from soil and preserved in our laboratories. The incubation conditions were established.The production was scaled up to 20 L and 200 L jar-fermenters through the utilization of on-line control techniques, and then thetechniques for batch fermentation of MTG were set up. The experimental results showed that the optimal processing param-eters achieved were 1.5% glucose and 1.0% soluble starch for the carbon source, 2.0% multi-peptone for the nitrogen source,initial pH 7.0, inoculum ratios of 5%-10%, that the following conditions were suitable during the MTG production with on-linecontrolled supervision: temperature 30℃±0.5℃, pH 6.6~6.9, aeration rate 0.8~1.1 vvm and agitation speed 300~400r/min.Under these conditions, the L-M168 strain could stably produce MTG higher than 2.75U/ml.

在首先采用我们自行设计的2L小型、便易的生化反应器,对我们研究室保藏的高产酶菌株链霉菌(Streptomyces sp.)WZFF.L-M168发酵生产微生物谷氨酰胺转胺酶(MTG)过程中发酵培养基的碳源、氮源和初始pH值的影响作用进行研究,确定培养条件后,逐级扩大发酵罐规模,在20L和200L发酵罐上以在线监控技术手段直接监测分析环境因素对MTG发酵生产的作用效果,确立各级发酵罐分批发酵的生产工艺条件。结果表明:碳氮源采用葡萄糖、淀粉和多价胨,初始pH值为7.0,接种量5%~10%,发酵过程中在线控制培养温度、pH、通气量和搅拌速度等各项参数指标分别为30±0.5℃、6.6~6.9,0.8~1.1vvm和200~400r/min较为适宜。在这优化技术条件下,200L发酵罐可以稳定生产酶活在2.75U/ml以上的MTG。

The production of extracellular lipase from Candida antarctica was studied in both shake-flask and 15 L fermenter. The nutrient medium composition and the operation conditions were optimized to obtain the optimal nutrient medium and the optimal operation conditions. Under the nutrient medium composition of bean powder 40 g·L -1, starch 15 g·L -1, bean oil 5 mL·L -1 and the operation conditions of temperature 24 ℃, pH6.0, gas amount 10.0 L·min -1, the period of fermentation was reduced to 54 h. The maximum...

The production of extracellular lipase from Candida antarctica was studied in both shake-flask and 15 L fermenter. The nutrient medium composition and the operation conditions were optimized to obtain the optimal nutrient medium and the optimal operation conditions. Under the nutrient medium composition of bean powder 40 g·L -1, starch 15 g·L -1, bean oil 5 mL·L -1 and the operation conditions of temperature 24 ℃, pH6.0, gas amount 10.0 L·min -1, the period of fermentation was reduced to 54 h. The maximum lipase yield of 19.2 U·mL -1 was achieved in 15 L fermenter.

用南极假丝酵母(C. antarctica DMS 3855)发酵生产胞外脂肪酶。通过摇瓶和15 L发酵罐发酵实验,研究了培养基成分和操作条件,得到较优的培养基组成和发酵生产的工艺条件。在培养基组成:豆粉40 g·L-1,淀粉15 g·L-1,豆油5 mL·L-1等;操作条件:温度24 ℃,初始pH6 0,通气量10 0 L·min-1,发酵周期缩短到54 h。由15 L发酵罐生产酶液的酶活达到19 2 U·mL-1。

It is very important to study how to produce high yield ice nucleation-active protein by changing the fermentation process condition of Xanthomonas ampelinaTS206 to make use of the ice nucleation-active bacteria in the industrial products. The fermentation process conditions of the ice nucleation-active bacteria Xanthomonas ampelina TS206 were studied in this paper. The results showed that the optimal culture temperature was 18℃, the volume of fermented broth 160ml/L, the optimal initial pH8.0 and the seed volume...

It is very important to study how to produce high yield ice nucleation-active protein by changing the fermentation process condition of Xanthomonas ampelinaTS206 to make use of the ice nucleation-active bacteria in the industrial products. The fermentation process conditions of the ice nucleation-active bacteria Xanthomonas ampelina TS206 were studied in this paper. The results showed that the optimal culture temperature was 18℃, the volume of fermented broth 160ml/L, the optimal initial pH8.0 and the seed volume 3%. Through measuring the growth curve of the bacteria we could learn that when the culture time arrived at 48h, the bacteria curve reached the increased logarithmic phase. The culture bacteria by this optimal fermentation condition the dry weight has increased 37.7% without decreasing the ice nucleation active. The concentration of the bacteria in the fermented broth is 2.87×1010/ml.

优化冰核活性细菌XanthomonasampelinaTS206实验室水平发酵生产的工艺条件为将冰核活性细菌应用在工业化生产提供了重要的技术资料。本文对冰核活性细菌XanthomonasampelinaTS206实验室水平的发酵生产工艺条件进行了系统的研究,结果表明这种冰核活性细菌的最适培养条件为:培养温度18℃,装液量40ml/250ml,摇床转速180r/min,接种量3%,发酵液的初始pH8。实验还确定了冰核活性细菌在发酵48h时达到对数生长期。采用上述发酵条件培养XanthomonasampelinaTS206可以使细菌在冰核活性不降低的条件下发酵液的菌体浓度达到2.87×1010个/ml,菌体干重增加了37.7%。

 
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