Methods: The isolation of normal rat hepatic stellate cells was established with collagenase in situ liver recirculating perfusion. Hepatic stellate cells were purified by using density gradient centrifugation with 18% Nycodenz.
This reserch was studied on the Breviscapine intestinal absorption dynamics first-time with 3 methods : everted sac technique, body circulatory perfusion technique and Caco-2 cell method.
Methods The hepatocytes were isolated by in-situ pre-perfusion and collagenase circulatory perfusion of rat livers. The effects of LPS associated with IFN-r, TNF a and IL-1 βor IL-6 on NOS activity, cGMP, and NO2-+NO3- were observed in hepatocytes, respectively.
Methods Rat hepatocytes were isolated by two-step in situ preperfusion and collagenase circulatory perfusion. The effects of hepatopoietin (HPN), nicotinamide (NA) and dimethyl sulfoxide (DMSO) on DNA synthesis, mitotic activity, morphology (under inverted microscope) and utrastructure (under TEM) of the hepatocytes were investigated in chemically defined culture medium.
All rats were allowed normal chow on the 1st postoperative day(POD). On the POD6,the intestinal glucose absorption data per unit length as well as per unit weight of ileum were measured by in vivo circulatory perfusion experiment.
On postoperative day 6,the morphological changes of the residual jejunum and ileum,the expression of proliferating cell nuclear antigen(PCNA),and the mRNA expressions of Na-D-glueose cotransperters (SGLT1) and peptide cotransporters (PEPT1) were determined. The intestinal glucose absorption data per unit length as well as per unit weight of ileum were measured by in vivo circulatory perfusion experiment.
The results shown that the absorption rate were 84.74% and 81.54% after nimodipine perfusion for 3 h in with and without the bile duct ligated,respectively(P>0.05).
RESULTS It was found that infusion of FDP (5 mmol/ L) caused much higher of total mechanical work of heart than control group (P<0.05) in both anoxia and glucose-free perfusions, and the amounts of FDP loss in perfusate were (16.2± 2.8)% and (20. 1± 3.7)% respectively after 40 min recyclecal perfusion.
METHODS Cyclical perfusion was used to acutely detach the mature guinea pig myocytes into single cells. The CK,LDH activity in a medium of myocardial cells was measured for 30,60,120 and 180 min after the effect of SOV at 1,10, 100 μmol·L -1 and 1 mmol·L -1 . Rates of cell viability and apoptosis were also measured. The protein content and Na +,K + ATP enzyme activity of the myocardial cells were measured as well.
Methods: The method for isolating of normal rat HSC was established with collagenase in situ liver recirculation perfusion, and 18%Nycodenz density gradient centrifugation.
No significant change in energy charge of ventricular tissue was observed after a thirty minute recirculating perfusion with palmitate when compared with the energy charge of hearts perfused with palmitate plus 11 mM glucose.
The detoxification of soman (1,2,2-dimethylpropyl methylphosphonofluoridate) was measured in rat livers, using hemoglobin-free, non-recirculating perfusion in situ.
Methods: Nine term human placentae were obtained immediately after delivery with maternal consent and a 2-h non-recirculating perfusion of a single placental cotyledon was performed.
Nonanticoagulated human blood was perfused across injured (air-insufflated) arterial and venous surfaces in a recirculating perfusion system at shear rates of 500 and 1500/sec.
The generally better tissue oxygenation, the reduction of the blood's viscosity and the increased circulatory perfusion all also favor a prophylaxis against deep vein thrombosis.
循环灌流
recirculating perfusion
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