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超微结构定位
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  ultrastructural localization
     ULTRASTRUCTURAL LOCALIZATION OF 185 kDa AND 82/41 kDa PROTECTIVE ANTIGENS IN PLASMODIUM FALCIPARUM, FCC1/HN
     恶性疟原虫FCC1/HN株185 kDa和82/41 kDa保护性抗原的超微结构定位
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     Ultrastructural Localization of Echinococcus granulosus 66 kDa Antigen
     细粒棘球绦虫66kDa抗原的超微结构定位
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     ULTRASTRUCTURAL LOCALIZATION OF 145/102 kDa ANTIGENS IN ERYTHROCYTIC STAGES OF PLASMODIVM FALCIPARUM
     恶性疟原虫红内期145/102 kDa抗原的超微结构定位
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     The ultrastructural localization of the 145/102 kDa antigens recognized by the possible protective monoclonal antibody (MeAb) M26-32 in erythrocytic stages of Plasmodium falci-parum, FCC1/HN, in vitro, was investigated by immuno-electron microscopy with LR White resin embedding and colloidal gold probe cytochemistry techniques.
     用LR White低温包埋法及胶体金标记免疫电镜细胞化学技术对保护性单克隆抗体M26-32识别的体外培养的恶性疟原虫FCC1/HN株红内期145/102 kDa抗原进行超微结构定位研究。
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     STUDY ON ULTRASTRUCTURAL LOCALIZATION AND ACTIVITY OF THE LYMPHOPROTEASE OF HUMAN PELVIC LYMPH NODES
     人盆部淋巴结淋巴细胞酶超微结构定位与活性研究
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  “超微结构定位”译为未确定词的双语例句
     Ultrastructural Location of the Enzyme in Lymphocytes of the Human Celiac Lymph Nodes
     人腹腔淋巴结淋巴细胞酶超微结构定位
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     The distribution of phospholipid and Ca2+ of rabbits lungs was quantitatively analysed by ultrastructual location method.
     采用磷脂和Ca2+超微结构定位方法,对肺磷脂和Ca2+的分布进行定位定量观察。
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     The author studied localization and activities of ATPase, G 6 Pase, 5′ Nucleotidase in lymphocytes of the human peripheral blood by using the electron enzyme cytochemical method.
     应用电镜酶细胞化学方法研究了人外周血淋巴细胞ATP酶、G6P酶、5′ND酶的超微结构定位与活性。
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     The distribution of Na~+ and Cl~- in wheat leaf mesophyll cells from plants grown in the solution culture with 200m tool NaCl was studied by means of electron microscopy and X-ray microanalysis.
     小麦经200mmol NaCl溶液培养3天后,采用改进的焦锑酸钾方法对叶肉细胞中Na~+及Cl~-进行超微结构定位
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     Conclusion Colloid gold labelling and immunoelectron microscope technique is valid sit method in human colorectal cancer cells GST-Pi.
     结论 免疫电镜金标记技术是大肠癌细胞GST -Pi超微结构定位的有效方法。
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  相似匹配句对
     ULTRASTRUCTURAL LOCALIZATION OF CARCINOEMBRYONIC ANTIGEN IN COLON CANCER
     结肠腺癌CEA的超微结构定位
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     Ultrastructural localization of phenoloxidase in the epidermal cell of the amphioxus Branchiostoma belcheri tsingtaunese
     文昌鱼表皮中酚氧化酶的超微结构定位
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     Video Text Location
     视频文本定位
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     Reasonable Orientation.
     合理定位
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     A Study on the Ultrastructure of Chondroblastoma
     软骨母细胞瘤的超微结构
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  ultrastructural localization
Ultrastructural localization of glucocerebrosidase in cultured Gaucher's disease fibroblasts by immunocytochemistry
      
Ultrastructural localization of brain-derived neurotrophic factor in rat primary sensory neurons
      
Using immunohistochemical and immunocytochemical techniques we have now investigated the ultrastructural localization of BDNF in the spinal cord of the rat.
      
Ultrastructural localization of butyrylcholinesterase in senile plaques in the brains of aged and Alzheimer disease patients
      
Different ultrastructural localization of VIP and prolactin in anterior pituitary cells of rats chronically treated with estroge
      
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The ultrastructural localization of adenosine triphosphatase(ATPase)activity in cotyledon cells of tomato was carried out by use of the cytochemical method of lead phosphate precipitation,and the changes in ATPase activity during chilling stress of the tomato seedlings were studied.The following experimental results have been obtained: 1.The ATPase activity in the cotyledon cells of tomato seedlings germinated and grown at 28℃.was located at plasmolemma,plasmodesmata,nucleoli and nuclear chromatin chloroplast...

The ultrastructural localization of adenosine triphosphatase(ATPase)activity in cotyledon cells of tomato was carried out by use of the cytochemical method of lead phosphate precipitation,and the changes in ATPase activity during chilling stress of the tomato seedlings were studied.The following experimental results have been obtained: 1.The ATPase activity in the cotyledon cells of tomato seedlings germinated and grown at 28℃.was located at plasmolemma,plasmodesmata,nucleoli and nuclear chromatin chloroplast lamellae,many sites of cell wall,and the surface of cell wall bordering the intercellular spaces and their inclusions(Fig.1—5). 2.When the tomato seedlings were subjected to chilling treatment for 4 hrs.at 5℃.,the ATPase activity in cotyledon cells(Fig.6)was indifferent from that of non-chilling treated seedlings.After chilling treatment for 12 hrs.at 5℃.,the reaction of ATPase activity at plasmolemma,and in cell wall and intercellular spaces was markedly reduced(Fig.7).though the high activity reaction of ATPase in nuclei and at chloroplast lamellae was still maintained.When the tomato seedlings were subjected to chilling stress for 24 hrs.at 5℃.,the ATPase activity at plasmolemma and in cell wall was almost inactivated,while the ATPase activity in nuclei and at chloroplast lamellae was only slightly lowered(Fig.9—11).These results indicated that the chilling injury may influence firstly on the ATPase activity of cell surface (plasmolemma and cell wall). 3.The role of intercellular spaces used as the passage of materials and the process and mechanism of chilling injury are discussed.

采用磷酸铅沉淀的细胞化学方法,对番茄子叶细胞内三磷酸腺苷酶(ATPase)活性进行了超微结构的定位,并研究了番茄幼苗在遭受冷害过程中 ATPase 活性的变化。结果指出:1.在28℃下萌发生长的番茄幼苗子叶细胞内的 ATPase 活性被定位于质膜、胞间连丝、核仁及核的染色质、叶绿体片层膜、部分细胞壁以及细胞间隙周围的细胞壁表面及其内含物上。2.当番茄幼苗遭受12小时冷(5℃)处理时,质膜、细胞壁及细胞间隙内的 ATPase 活性开始明显地降低,但细胞核和叶绿体片层膜上的 ATPase 仍保持高的活性反应。在冷处理24小时后,质膜与细胞壁的 ATPase 活性几乎完全丧失,而细胞核和叶绿体片层膜的 ATPase 活性仅开始减弱。这种情况揭示,冷害可能首先损伤细胞表面(质膜与壁)的 ATPase 活性。3.讨论了细胞间隙作为养料运输通道的作用以及冷害的一种可能过程与机理。关于高等植物细胞内三磷酸腺苷酶(ATPase)活性的细胞化学的超微结构定位,以往主要集中于维管束的韧皮部和根尖细胞的研究。不久前,我们报道了冬小麦分蘖节细胞内 ATPase 活性的细胞化学定位叫,初步揭示 ATPase 的活性变化与植...

采用磷酸铅沉淀的细胞化学方法,对番茄子叶细胞内三磷酸腺苷酶(ATPase)活性进行了超微结构的定位,并研究了番茄幼苗在遭受冷害过程中 ATPase 活性的变化。结果指出:1.在28℃下萌发生长的番茄幼苗子叶细胞内的 ATPase 活性被定位于质膜、胞间连丝、核仁及核的染色质、叶绿体片层膜、部分细胞壁以及细胞间隙周围的细胞壁表面及其内含物上。2.当番茄幼苗遭受12小时冷(5℃)处理时,质膜、细胞壁及细胞间隙内的 ATPase 活性开始明显地降低,但细胞核和叶绿体片层膜上的 ATPase 仍保持高的活性反应。在冷处理24小时后,质膜与细胞壁的 ATPase 活性几乎完全丧失,而细胞核和叶绿体片层膜的 ATPase 活性仅开始减弱。这种情况揭示,冷害可能首先损伤细胞表面(质膜与壁)的 ATPase 活性。3.讨论了细胞间隙作为养料运输通道的作用以及冷害的一种可能过程与机理。关于高等植物细胞内三磷酸腺苷酶(ATPase)活性的细胞化学的超微结构定位,以往主要集中于维管束的韧皮部和根尖细胞的研究。不久前,我们报道了冬小麦分蘖节细胞内 ATPase 活性的细胞化学定位叫,初步揭示 ATPase 的活性变化与植物抗寒性有密切关系。番茄等喜温植物在冷害中的细胞超微结构,呼吸作用和一些酶(如过氧化物酶,过氧化氢酶及吲(口朶)乙酸氧化酶)活性的变化,已有一些报道,提出膜可能是冷害损伤的最初部位。然而冷害究竟首先是损伤膜的拟脂成分,还是损伤膜的蛋白质成分,或者是二者同时遭到破坏,目前的实验证据还十分缺乏。ATPase 是膜束缚的一种功能性蛋白质,我们试图通过探索它在寒害中的活性变化,为阐明寒害机理提供实验依据。

Ultrastructural localization of acid phosphatase in Giant Cell Tumor (GCT) of bone is one of the important methods for the investigation of the nature, interrelationship and histogenesis of the three types of cells in GCT.We have done a study on the electron microscopic localization of acid phosphatase in five cases of GCT and also in the liver of rats and mice. At the same time, histochemical study of acid phosphatase according to Gomori's lead sulfate method was done and the specimens were observed under the...

Ultrastructural localization of acid phosphatase in Giant Cell Tumor (GCT) of bone is one of the important methods for the investigation of the nature, interrelationship and histogenesis of the three types of cells in GCT.We have done a study on the electron microscopic localization of acid phosphatase in five cases of GCT and also in the liver of rats and mice. At the same time, histochemical study of acid phosphatase according to Gomori's lead sulfate method was done and the specimens were observed under the light microscope for comparison.This paper reports the effect of glutaldehyde fixation on the demonstration of acid phosphatase in GCT. It is suggested that the time for fixation should not exceed 24 hours and the specimens should be kept at 4℃. At the same time we compared the results of using tris/maleate buffer and acetate buffer in the ultrastructural localization of acid phosphatase in GCT and liver of rats and mice, and we found that acetate buffer gave better results.We found that direct observation of the ultrathin sections without staining with lead citrate showed the details of ultrastructure better than sections stained with lead citrate.

在骨巨细胞三种类型细胞的性质和组织发生相互关系的研究方面。酸性磷酸酶超微结构定位是很重要方法之一。但国内有关骨巨细胞瘤酸性磷酸酶超微结构定位未曾见有报道。我们对五例骨巨细胞瘤,大白鼠肝脏及小白鼠肝脏进行了酸性磷酸酶电镜定位;同时,用Gomnri氏硫化铅法的酸性磷酸酶组织化学方法进行观察作为比较。本文报告戊二醛固定液对显示酸性磷酸酶活性的影响,提出固定时间不得超过24小时,并应置于4℃冰箱。此外对trise/maleate缓冲及醋酸缓冲液进行比较,发现在骨巨细胞瘤,大白鼠及小白鼠肝脏电镜定位醋酸缓冲液比较好。在观察酸性磷酸酶超微结构时,超薄切片可不经过柠檬酸铅染色而直接观察,比经过柠檬酸铅染色,结构更为清晰。

Standard lead precipitation procedures have been used to examine the localization of ATPase activity during cytomixis in pollen mother cells of Lilium davidii var.willmottiae (Wilson) Roffill.Before cytomixis,cells at this stage of development show ATPase activity on plasma membrane,in the endoplasmic reticulum,dictyosomes, plastids,plasmodesmata,and in part of the groundplasm;however,there is no ATPase activity on the chromatin and nucleolus.During cytomixis,the chromatin substance begin to transfer from one...

Standard lead precipitation procedures have been used to examine the localization of ATPase activity during cytomixis in pollen mother cells of Lilium davidii var.willmottiae (Wilson) Roffill.Before cytomixis,cells at this stage of development show ATPase activity on plasma membrane,in the endoplasmic reticulum,dictyosomes, plastids,plasmodesmata,and in part of the groundplasm;however,there is no ATPase activity on the chromatin and nucleolus.During cytomixis,the chromatin substance begin to transfer from one cell to an adjacent cell,reaction product indicating ATPase activity is observed associated with the chromatin and nucleolus.ATPase activity is also found with the cistenae of both endoplasmic reticulum and dictyosomes,and some plastids.There is no deposition of ATPase reaction product associated with the plasm membrane and intercellular spaces.After cytomixis,the chromatin is little or no depo- sition of enzyme reaction product.ATPase activity,however,is consistenlly found within the intercellular space and on the plasm membrane,and also occur in the endo- plasmic reticulum,dictyosome and plastid. The presence or absence of ATPase activity in the cell structure of pollen mother cells before,during or after cytomixis is discussed in relation to the active uptake or export of water for short-distance transport.It is also suggested that the intensive ATPase activity in the nucleus during cytomixis of pollen mother cells is evidence for a transport system involved in the active movement of the intercellular migrating chro- matin substance.

用标准的磷酸铅沉淀的细胞化学方法,对百合花粉母细胞间染色质穿壁运动期间及其前后三个时期中的腺苷三磷酸酶(ATP 酶)活性进行了超微结构的定位。结果表明:(1)在穿壁前,ATP 酶活性主要定位于质膜、胞间连丝及细胞间隙;在内质网、高尔基体、质体和某些局部的基质(groundplasm)中,也表现有 ATP 酶活性反应的产物;但在染色质和核仁中,一般都没有这种反应。(2)在穿壁时,染色质从一个细胞穿壁转移到另一个相邻细胞,同时看到染色质和核仁内出现密集的 ATP 酶活性反应产物;在内质网和高尔基体的腔内以及质体的片层上也产生明显的 ATP 酶活性反应;而在质膜、胞间连丝及细胞间隙内 ATP 酶活性明显降低,甚至看不到明显的活性反应。(3)在穿壁后,质膜及细胞间隙中又产生明显的 ATP 酶活性反应产物,但核内染色质上的 ATP 酶活性则显著降低,而核仁内则仍有较高的活性。同前二个时期一样,内质网、高尔基体和质体上的 ATP 酶仍表现明显的活性反应。最后讨论了三个不同发育时期 ATP 酶活性及其分布部位的改变与染色质胞间转移的关系。

 
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