助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   亲和力常数 的翻译结果: 查询用时:0.86秒
图标索引 在分类学科中查询
所有学科
基础医学
生物学
更多类别查询

图标索引 历史查询
 

亲和力常数
相关语句
  affinity constant
     The affinity constant of F(ab′)_2 fragments and monoclonal antibody were 2.95×10~ -9 mol/L and 1.52×10~ -10 mol/L, respectively.
     单抗2G2F(ab′)2片段的亲和力常数为2.95×10-9mol/L,单抗2G2的亲和力常数为1.52×10-10mol/L。
短句来源
     The antibody subclass is IgG2a,affinity constant is 2.9×10-11 mol/L,molecular weight is 150 kD,recovery is 81.5%~125.0%,cross-reaction <1%。
     得到了抗微囊藻毒素单克隆抗体细胞株,命名为G5,抗体亚类为IgG2a,亲和力常数2.9×10-11mol/L,分子量150KD,加标回收率在81.5%~125.0%之间,与其他结构类似物的交叉反应率<1%。
短句来源
     Also, the four mAb have isotypes of IgG 1/κ、IgG 1/κ、IgG 2a /κ、IgG 2a /λ,respectively,and affinity constant is 1.68×10 8 L/mol in C4G1,1.3×10 8 L/mol in C2H6.CL mAb have specificity integrated with CL conjugated antigen that determined by WestGold protein blotting assay.
     同种型分别为IgG1/κ、IgG1/κ、IgG2a/κ、IgG2a/λ。 C 4G1的亲和力常数K =1 6 8× 10 8L/mol,C 2H6的亲和力常数K =1 3× 10 8L/mol。
短句来源
     The monoclonal antibody produced by the hybridoma cell was tested for subtypes and designated as IgG_1.The reference working concentration was 1∶1.6×10~6,and its affinity constant was 8×10~(-8)mol/L.
     该细胞株所分泌的单克隆抗体Ig亚类为IgG1,参考工作浓度为1∶1.6×106,亲和力常数为8×10-8mol/L。
短句来源
     Results Three hybridoma cell strains were obtained and named EGF-B 2, EGF-C 7, and EGF-A 8, which produced monoclonal antibodies specifically against rhEGF. The isotypes of the monoclonal antibodies were IgG 1κ, IgG 1λ, and IgG 3κ and the affinity constant were 2.76×10 10, 3.2×10 9, and 1.45×10 9, respectively.
     结果 获得 3株产生针对人表皮生长因子的单克隆抗体细胞株EGF B2 、EGF C7、EGF A8,Ig亚分别为IgG1κ型 ,IgG1λ型 ,IgG3 κ型 ,亲和力常数分别为 2 .76× 10 10 、3.2× 10 9、1.4 5× 10 9。
短句来源
更多       
  affinity constants
     These McAbs reacted strongly with Ⅳ-CL moleculeswith the affinity constants(Kaff) of 6.6×108M-1~6.0×1010M-1 and did not have cross-reactivity with other types of collagen and irrelevant proteins.
     其亲和力常数Kaff=6.6×108M(-1)~6.0×10(10)M(-1)。 它们均能特异性地识别人Ⅳ型胶原蛋白。
短句来源
     the affinity constants (Kaffi),2D4:5. 41±0. 94 ×10-10mol/L and 4F4:1. 37 ±0. 33×10-9mol/L.
     亲和力常数(K_(affi)),2D_4为5.41±0.94×10~(-10)mol/L,4F_4为1.37±0.33×10~(-9)mol/L。
短句来源
     Affinity constants of Z2A4 and Z8D6 were 5.52×108 and 4.68×109 mol/L respectively.
     抗体与其它霉菌毒素无交叉反应(交叉反应率 <1% ) ,具有较高特异性 ; 抗体亲和力常数Z2A4和Z8D6分别为 5 5 2× 10 8和 4 6 8×10 9mol L。
短句来源
     The indirect ELISA titers of the three strains were 1∶4×103,1∶8×104 and 1∶1×103,and the affinity constants of them were 3.96×108,4.04×109 and 1.26×108 L/mol,respectively.
     亲和力常数分别为3.96×108、4.04×109和1.26×108L/mol;
短句来源
     In this paper, a simple method (modified Friguet' s) was reported to directly measure the experimental affinity data of monoclonal antibody in ascites by means of ELISA and to accurately determine the corresponding affinity constants of monoclonal antibody by amendment of underestimation of affinity constants due to the intrinsic properties of Friguet' s, and shows convenience and reliability.
     本实验参照Friguet氏利用酶联免疫吸附试验(ELISA)测定抗体亲和力的基本原理,建立了改良测定法,能准确且方便地直接测得腹水中单克隆抗体的亲和力常数
短句来源
  “亲和力常数”译为未确定词的双语例句
     The affinity for pres2 antigen was 2.16± 0.20× 10 8 L/mol.
     抗体亲和力常数为 (2 .16± 0 .2 0 )× 10 8L/mol。
短句来源
     The Km and Vmax of striatal synaptosomal [~3H]DA uptake were about 0.23/μmol and 1.2 nmol/g tissue (5 min) at 25℃ respectively.
     突触体对[~3H]DA的亲和力常数Km为0.23μmol。 最大摄取速率Vmax为1.2 nmol/g(5 min)。
短句来源
     The coefficients of variation was 4.18% within the strips,and 5.21% among the level of 0.30 μg/kg,2.10 μg/kg and 10.40% was 98.63%,94.56% and 112.05% respectively.
     试剂盒的条内变异系数为4.18%,条间变异系数为5.21%,抗体亲和力常数为1.52×10-10mol/L; 对玉米3个加标水平的平均回收率分别为98.63%,94.56%和1 12.05%;
短句来源
     Comparison of their relative affinity was 1A3>1B5>1A5.
     亲和力常数依次为1.0×109M-1、0.5×107-1和2.0×108-1。
短句来源
     Results:Two TTX-specific mAbs with a titer as high as 10 5 to 10 6 in dilution were developed and named as 1E4 and 3C11. Both of them could react to TTX with the IC 50 values of 3.2×10 -6 mol/L and 9.9×10 -7 mol/L,respectively.
     具有TTX结合活性 ,其IC50 值分别为 3.2× 10 _6 mol L和 9.9× 10 _7mol L ; 亲和力常数 (Kaff)分别为 3.0× 10 6 L mol和 2 .8× 10 6 L mol。
短句来源
更多       
查询“亲和力常数”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  affinity constant
The affinity constant KA of LPS with serum albumin, hemoglobin, chitosan and lysozyme was 2.36 × 107, 2.03 × 108, 7.58 × 106, 2.82 × 104 L·mol-1, respectively.
      
Based on sorption isotherms for Au(III), Pd(II), Rh(III), and Ru(IV), the conditional affinity constant values for HA sorption centers with respect to ions of these metals were calculated by the method of quantitative physicochemical analysis.
      
The affinity constant for the antibody was estimated by solid-phase enzyme-linked immunoassay to be 7.7 × 107 ± 1.5 × 107 M-1.
      
The empty site affinity constant decreased by 51% from 1.0×108 l/mol at 4°C to 4.9×107 l/mol at 37°C, whereas the total receptor concentration remained unaltered at both temperatures.
      
Saturation experiments (n=10) indicate the presence of one homogeneous population of high affinity binding sites with an affinity constant (Kd) of 8-13 nM and a maximum binding capacity (Bmax) of 47±3.5 pmol/mg protein.
      
更多          
  affinity constants
Affinity constants were determined for all antibodies, and their specificity for various structural forms of the enzyme (native peroxidase, apoperoxidase, and denatured peroxidase) were assessed by competitive enzyme immunoassay.
      
The affinity constants of protein A binding to the CH2-CH3 domain interface demonstrated differences between serum IgG1 and tumor associated IgG1 that did not exceed 3-8-fold.
      
After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4·107 and 1.2·106 M-1 were chosen.
      
The affinity constants of the resulting scFv were in the range of 2 × 104 to 1.8 × 107 M-1.
      
Reduced number of beta- and alpha-receptor binding sites without changes in the affinity constants were observed in diabetic myocardium.
      
更多          


Over the last 9 years a series of RIA and ELISA for ABA, IAA, Cytkinins, gibberellins and other PGRs have been developed. Four important technique aspects of PGRIA are as follows.(1) Design and synthesis of hapten-protein conjugates (immunogens) of defined structure. (2) Preparation of a specific antiserum or ascites fluid by immunizing rabbits or mice respectively.(3) Preparation of a labeled antigen or antigen derivative which exhibits the same or comparable affinity to the antibodies as the unlabeled antigen.(4)...

Over the last 9 years a series of RIA and ELISA for ABA, IAA, Cytkinins, gibberellins and other PGRs have been developed. Four important technique aspects of PGRIA are as follows.(1) Design and synthesis of hapten-protein conjugates (immunogens) of defined structure. (2) Preparation of a specific antiserum or ascites fluid by immunizing rabbits or mice respectively.(3) Preparation of a labeled antigen or antigen derivative which exhibits the same or comparable affinity to the antibodies as the unlabeled antigen.(4) Selection of optimum assay conditions.

当前,西德等国正在加速开发与利用免疫技术对植物激素进行定量、定位与纯化。本文以ABA与GA_3的RIA为例,阐述竞争性PGRIA的原理与四项技术要点(设计与合成特定的免疫原、制备单克隆或多克隆抗体、制备标记抗原和选择最佳测试条件),预期PGR抗体的亲和力常数(Ka)与专一性均将有显著的提高。作为乙烯前体的ACC、其他激素的前体、降解物与结合物都将被合成为免疫原,并取得相应的抗体。PGRIA将成为一项高技术。

Effects of some tetrahydroisoquinoline alkaloids on rat striatal synaptosomal uptake and vesicular storage of [~3H] dopamine ([~3H]DA) have been investigated in vitro. The Km and Vmax of striatal synaptosomal [~3H]DA uptake were about 0.23/μmol and 1.2 nmol/g tissue (5 min) at 25℃ respectively. In our experiments, d-THP, 1-THP, 1-SPD, nomifensine, amphetamine and haloperidol all significantly decreased the accumulation of [~3H] DA into the striatal synaptosomes. The order of inhibitory potency (IC_(50)) of these...

Effects of some tetrahydroisoquinoline alkaloids on rat striatal synaptosomal uptake and vesicular storage of [~3H] dopamine ([~3H]DA) have been investigated in vitro. The Km and Vmax of striatal synaptosomal [~3H]DA uptake were about 0.23/μmol and 1.2 nmol/g tissue (5 min) at 25℃ respectively. In our experiments, d-THP, 1-THP, 1-SPD, nomifensine, amphetamine and haloperidol all significantly decreased the accumulation of [~3H] DA into the striatal synaptosomes. The order of inhibitory potency (IC_(50)) of these compounds was nomifensine (0.05βmol)>d-THP(0.44 μmol), amphetamine(0.27 μmol)>1-SPD (2.2 μmol), haloperidol(3.2 μmol)>1-THP(18.5 μmol), d-THP was about 20 times more potent than 1-THP. In kinetic study, the inhibition of both d-THP and nomifensine appeared to be competitive, d-THP, similar to reserpine, also markedly inhibited the accumulation of [~3H] DA into vesicular store in a dose-dependent manner. Our results indicate that THP not only can inhibit potently the uptake of [~3H]DA into rat striatal synaptosomes but also act directly on the vesicles to reduce the [~3H]DA storage like reserpine.

本文报道四氢巴马汀(THP)等异喹啉生物碱对[~3H]DA摄取的作用。突触体对[~3H]DA的亲和力常数Km为0.23μmol。最大摄取速率Vmax为1.2 nmol/g(5 min)。d-THP,1-SPD和1-THP对突触体摄取[~3H]DA均有抑制作用,其IC_(50)分别为0.44,2.24和18.5μmol。d-THP的作用比1-THP约强20倍。nomifensine,苯丙胺和氟哌啶醇亦能有效地抑制[~3H]DA摄取(IC_(50)分别为0.05,0.27和3.18μmol)。动力学研究表明,d-THP和nomifensine为DA摄取的竞争性抑制剂。用低渗溶液处理溶胀的方法研究药物对递质贮存的作用发现,与利血平相似,d-THP显著降低贮存囊泡[~3H]DA含量并使囊泡与突触体[~3H]DA含量之比明显减小。实验结果表明,THP等能抑制突触体摄取,并直接作用于贮存囊泡抑制[~3H]DA贮存,因此具有利血平样排空作用。

Antibody affinity influences the ant igen-antibody reaction in vivo and in vitro. It is important to routinely determine the antibody affinity in order to elucidate the impacts of this antibody characteristics on the immunoassay and the pathogenetic mechanisms of human diseases. In this paper, a simple method (modified Friguet' s) was reported to directly measure the experimental affinity data of monoclonal antibody in ascites by means of ELISA and to accurately determine the corresponding affinity constants...

Antibody affinity influences the ant igen-antibody reaction in vivo and in vitro. It is important to routinely determine the antibody affinity in order to elucidate the impacts of this antibody characteristics on the immunoassay and the pathogenetic mechanisms of human diseases. In this paper, a simple method (modified Friguet' s) was reported to directly measure the experimental affinity data of monoclonal antibody in ascites by means of ELISA and to accurately determine the corresponding affinity constants of monoclonal antibody by amendment of underestimation of affinity constants due to the intrinsic properties of Friguet' s, and shows convenience and reliability.

抗体亲和力对体内外免疫反应有重要影响,为阐明抗体亲和力在免疫学检测及疾病发生发展机理中的作用,需要建立简单准确的测定抗体亲和力的方法。本实验参照Friguet氏利用酶联免疫吸附试验(ELISA)测定抗体亲和力的基本原理,建立了改良测定法,能准确且方便地直接测得腹水中单克隆抗体的亲和力常数

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关亲和力常数的内容
在知识搜索中查有关亲和力常数的内容
在数字搜索中查有关亲和力常数的内容
在概念知识元中查有关亲和力常数的内容
在学术趋势中查有关亲和力常数的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社