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通用型血
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  universal donor blood
     The mPEG-BTC could camouflage the rare blood group antigens on RBC surface efficiently and had no influence on structure and function of modified RBCs. It will provide a useful strategy for preparation of universal donor blood.
     综上所述,本研究说明,mPEG-BTC修饰红细胞,能有效遮蔽红细胞的稀有血型抗原,不影响红细胞的结构功能,是制备通用型血的一条有效途径。
短句来源
     Conclusion:This work is the basis for further study of "universal donor blood preparation" in clinical transfusion.
     结论 :本研究为B→O血型改造制备的通用型血进一步的临床应用奠定了基础。
短句来源
  “通用型血”译为未确定词的双语例句
     Detection of minimal amount of residual α-galactosidase involved in conversion of blood type B to O
     B→O血型改造制备通用型血过程中残留工具酶的检测
短句来源
     Modified or camouflaged red blood cells surface antigen in vitro—universaldonor blood ?
     体外化学修饰红细胞——通用型血?
短句来源
     Chemical camouflage mPEG also converted the red blood celis into universal type for transfusion.
     用mPEG包裹人血红细胞制备的通用型血,也能应用于临床输血。
短句来源
     Alpha galactosidase is an important enzyme for seroconversion from group B to universal group O of human red blood cells.
     α-半乳糖苷酶是实现 B→O血型转变、制备通用型血的关键工具酶 .
短句来源
     Galactosidase plays an important role in red blood cell seroconversion from group B to group O.
     α_半乳糖苷酶是实现B→O血型转变、制备通用型血的关键工具酶。
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  相似匹配句对
     Modified or camouflaged red blood cells surface antigen in vitro—universaldonor blood ?
     体外化学修饰红细胞——通用?
短句来源
     Discussion on a general-purposed ultrasonic generator
     通用功率超声发生器
短句来源
     (2) blood stasis type;
     ② ;
短句来源
     (3)D shape.
     D
短句来源
     (2)C shape;
     C ;
短句来源
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This paper describes the molecular structure on the surface of human red blood celis as well as their coding genes. The paper also reviews the research advances in the field of blood conversion. The enzyme ot-galactosidase isolated from green coffee bean could convert the red blood celis of Blood group B into blood group O type. The experimental results in vitro conformed the integrity, normai viability of the converted red blood celis of blood group B, and showed that the converted celis exhibited immune response...

This paper describes the molecular structure on the surface of human red blood celis as well as their coding genes. The paper also reviews the research advances in the field of blood conversion. The enzyme ot-galactosidase isolated from green coffee bean could convert the red blood celis of Blood group B into blood group O type. The experimental results in vitro conformed the integrity, normai viability of the converted red blood celis of blood group B, and showed that the converted celis exhibited immune response of blood group O type. Phase I clini-cal trials demonstrated that the converted red blood celis of blood group B could infuse safely to both blood group A and blood group O recipients. Chemical camouflage mPEG also converted the red blood celis into universal type for transfusion.

本文描述了人血红细胞表面分子结构及其基因,介绍了有关血型转变的研究进展。从绿色咖啡豆中制备的α-半乳糖苷酶可以将B型血转变为O型血。体外研究证明了转型后的红细胞的完整性,正常活存率,呈现O型血的免疫特征。人Ⅰ期临床实验证明,转型后的B型红细胞可以安全地输给A型和O型血的自愿受试者。用mPEG包裹人血红细胞制备的通用型血,也能应用于临床输血。

Polyethylene glycol (PEG) and its derivatives are one kind of promise compounds for application. In this article, the increace of sensitivity of detection for blood type and pathogenic microbe antigen by PEG precipitation, the preparation of universal blood by mPEG camouflage of red blood cell antigenic determinants and the development of blood substitute by conjugation of PEG on hemoglobin as well as by microencapsulation of hemoglobin in PEG liposomes were reviewed.

聚乙二醇及其衍生物是一类具有重要应用前景的化合物。本文综述了用聚乙二醇沉淀提高血型和病原微生物抗原检测的灵敏度及用甲氧基聚乙二醇遮蔽红细胞血型抗原决定簇和制备通用型血的研究,并综述了用聚乙二醇修饰血红蛋白及用含聚乙二醇衍生物的脂质体包封血红蛋白生产血液代用品等方面的研究。

Alpha galactosidase is an important enzyme for seroconversion from group B to universal group O of human red blood cells.Its cDNA cloned by RT PCR from green (unroasted) Catimor coffee beans collected in Hainan Province (China),was constructed into the vector pPIC9K designed for secretion expression in P.pastoris. The construct was then transformed into P.pastoris strain GS115. Followed by expression selection,a recombinant strain that exhibited high level production of alpha galactosidase was obtained.After...

Alpha galactosidase is an important enzyme for seroconversion from group B to universal group O of human red blood cells.Its cDNA cloned by RT PCR from green (unroasted) Catimor coffee beans collected in Hainan Province (China),was constructed into the vector pPIC9K designed for secretion expression in P.pastoris. The construct was then transformed into P.pastoris strain GS115. Followed by expression selection,a recombinant strain that exhibited high level production of alpha galactosidase was obtained.After 7 days of continuous methanol induction,it was observed that the total secreted proteins in culture supernatants reached up to 1.2 mg/ml,migrating as the main bands of 41 kD on SDS PAGE.Alpha galactosidase activity was up to 18 U/ml when assayed using p nitrophenyl alpha D galactopyranoside as substrate.The enzyme was then purified and used to treat group B red blood cells for blood conversion.Preliminary evidence suggested that the recombinant alpha galactosidase could convert these cells serologically to group O.

α-半乳糖苷酶是实现 B→O血型转变、制备通用型血的关键工具酶 .利用反转录 PCR方法从中国海南 Catimor咖啡豆中克隆α-半乳糖苷酶 c DNA,插入嗜甲基酵母 P.pastoris分泌表达载体 p PIC9K中 ,转化 P.pastoris GS1 1 5,筛选高表达重组菌株 .经甲醇诱导表达 7d后 ,发酵液总蛋白分泌量约 1 .2 mg/ml,SDS- PAGE呈现约 41 k D特异表达带 ,与专一性底物对 -硝基 -苯基 -α- D-吡喃半乳糖苷反应证明 ,表达产物具有 α-半乳糖苷酶活性 ,最高达到 1 8U/ml.初步实验表明 ,表达的 α-半乳糖苷酶可酶解 B型红细胞 ,成功实现 B→O血型转变 .

 
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