Methods: The growth inhibition rates of human bladder cancer cell line BIU 87 by various concentration As 2O 3 were studied by MTT method, cell apoptosis of BIU 87 cells was observed by SABC immunohistochemical method.
Objective To observe the effects of photodynamic therapy (PDT) of metalloporphyrin on proliferation and the expression of bcl-2 and bax genes which regulated cell apoptosis in human bladder cancer cells and to study its mechanism.
Objective To study the effect of antisense VEGF gene transfection on the expression of VEGF in human bladder cancer cells with the help of adeno-associated virus-mediated vector (rAAV) and explore a new gene therapy strategy for bladder tumor.
Methods Different doses (0,20,100 μl) of antisense VEGF genes were transferred into human bladder cancer T24 cells by rAAV. Immunohistoche-mical and Western blot techniques were used to detect the expression of VEGF in T24 cells.
Expression of a mutant hTERT in human bladder carcinoma cell line T24 and its clinical significance
Apoptosis induced by ginsenoside Rg3 in a human bladder carcinoma cell line
Erythroid progenitor cells (BFUe: burst forming units-erythroid) are cultured in 1 ml of 0.35% agarose in IMDM with 30% FBS and conditioned medium from human bladder carcinoma cell line 5637 or recombinant interleukin 3.
Three-dimensional modeling of T-24 human bladder carcinoma cell line: A new simulated microgravity culture vessel
Stability of lectin binding properties expressed by human bladder carcinoma cell lines passaged in vitro or in nude mice
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
Conclusion: The constructed chimeric antibody was expressed successfully in eukaryotic cells, and the chimeric antibody had desired affinity against human bladder cancer cells.
The effects of EPI-CDMN associated with external pulsed electromagnetic fields (PEMFs) (10 mT) on killing human bladder cancer BIU-87 cells were studied by MTT assay and Annexin-V/PI double-labeled flow cytometry technique, respectively.