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   极化荧光 的翻译结果: 查询用时:0.037秒
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极化荧光
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  fluorescence polarization
     Conclusion The results indicated that the fluorescence polarization assay is stable, sensitive, reproducible and well suited for high throughput screening efforts.
     结论极化荧光检测方法适合于高通量筛选技术,具有较高的稳定性、灵敏性和可重复性。
短句来源
  fluorescence polarization-based
     Application of fluorescence polarization-based assay in high throughput screening
     极化荧光在高通量药物筛选中的应用
短句来源
     Identification of ligands for human LOX-1 through fluorescence polarization-based high throughput screening
     基于极化荧光方法的人LOX-1配体高通量筛选
短句来源
     Aim To develop a fluorescence polarization-based high throughput screening and identify ligands for human Lectin-like oxidized low-density lipoprotein receptor-1 (hLOX-1).
     目的建立以极化荧光为检测方法的高通量筛选技术,通过大规模筛选发现氧化型低密度脂蛋白受体1(LOX1)的天然配体。
短句来源
  相似匹配句对
     A technique for polarization analysis
     极化分析技术
短句来源
     Application of fluorescence polarization-based assay in high throughput screening
     极化荧光在高通量药物筛选中的应用
短句来源
     RESONANCE FLUORESCENCE
     共振荧光
短句来源
     Fluorescent filter
     荧光滤光片
短句来源
     Poling Effect on Fluorescence Life-Time of Doped PMMADR1
     极化取向对PMMA-DR1荧光寿命的影响
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  fluorescence polarization
Fluorescence Polarization Studies of Different Forms of Angiotensin-Converting Enzyme
      
The interaction of three forms of bovine angiotensin-converting enzyme (ACE) with the competitive peptide inhibitor lisinopril with a fluorescent label was studied by the fluorescence polarization technique.
      
Determination of the herbicide acetochlor by fluorescence polarization immunoassay
      
A fluorescence polarization immunoassay procedure was developed for determining the herbicide acetochlor from the group of chloroacetanilides.
      
Determination of the herbicide acetochlor by fluorescence polarization immunoassay
      
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  polarized fluorescence
These features of GroEL interaction with positively and negatively charged denatured proteins were confirmed by polarized fluorescence (fluorescence anisotropy).
      
Solutions of hydrated reversed micelles and mixtures of distillate cracking residue and light catalytic gas oil are studied by the methods of correlation spectroscopy of scattered light and polarized fluorescence, respectively.
      
Depolarization of excited114Cd 5s5p3P1 atoms induced by collisions with various molecular gases (N2, H2, D2, CO, CO2, CH4, C2H6, C2H4) has been investigated using polarized fluorescence spectroscopy.
      
Comparison with decay-time data brings out that deviation from the value obtained from absorption measurements is due to a loss of intensity in the a-polarized fluorescence as compared to what is expected from absorption data.
      
Theoretical results are presented which are appropriate for the analysis of the static polarized fluorescence experiment with oriented pigment molecules in spherical arrays (vesicles).
      
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Aim To develop a fluorescence polarization-based high throughput screening and identify ligands for human Lectin-like oxidized low-density lipoprotein receptor-1 (hLOX-1). Methods (Sequential ultracentrifugation) at 4 ℃ from normolipidemic fasting volunteers to obtain low density lipoprotein (LDL), which was modified by CuSO_(4) (5 μmol·L~(-1)) at 37 ℃ for 24 h. The assay was based on the interaction between receptor and ligand, and hLOX-1 was labeled by FITC and bound to its specific ligand, oxLDL. Different...

Aim To develop a fluorescence polarization-based high throughput screening and identify ligands for human Lectin-like oxidized low-density lipoprotein receptor-1 (hLOX-1). Methods (Sequential ultracentrifugation) at 4 ℃ from normolipidemic fasting volunteers to obtain low density lipoprotein (LDL), which was modified by CuSO_(4) (5 μmol·L~(-1)) at 37 ℃ for 24 h. The assay was based on the interaction between receptor and ligand, and hLOX-1 was labeled by FITC and bound to its specific ligand, oxLDL. Different reaction time and DMSO concentration were optimized to determine the stability and tolerance of fluorescence polarization (FP) assay. 3 200 compounds were screened in black 384-well microplate by FP-based competitive displacement assay, at excitation filter of 485 nm and emission filter of 530 nm. Z′ was used to assess the assay quality. Results The FP-based HTS was formatted in a 384-well microplate with a Z′ factor of 0.75, and three active compounds for hLOX-1 were identified with IC_(50) below 40 μmol·L~(-1) from total 3 200 compounds. Conclusion The results indicated that the fluorescence polarization assay is stable, sensitive, reproducible and well suited for high throughput screening efforts.

目的建立以极化荧光为检测方法的高通量筛选技术,通过大规模筛选发现氧化型低密度脂蛋白受体1(LOX1)的天然配体。方法密度梯度超速离心获得正常人血中低密度脂蛋白(LDL),然后用CuSO4(5μmol·L-1)修饰为氧化型低密度脂蛋白(oxLDL)。FITC标记hLOX1,以受体(LOX1)和配体(oxLDL)的相互作用为基础建立筛选模型,用极化荧光检测方法,在激发光485nm、发射光525nm,对3200个样品进行高通量筛选,并用Z′因子值评价实验。结果Z′因子值为0.75,根据建立的基于极化荧光的高通量筛选实验方法,发现3个化合物与hLOX1有较高的结合活性,IC50值小于31.6μmol·L-1。结论极化荧光检测方法适合于高通量筛选技术,具有较高的稳定性、灵敏性和可重复性。

 
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