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化学发光技术
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  chemiluminescence technique
     The most new ultrasensitive chemiluminescent photographic detectiondot-ELISA (CPD-Dot-ELISA) technique was developed by combining the chemiluminescence technique with Dot-ELISA.
     本文将化学发光技术与Dot-ELISA相结合,建立了一种新的、超灵敏的化学发光自显影-Dot-ELISA (Chemiluminescent Photographic Detection-Dot-ELISA,CPD-Dot-ELISA)技术。
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     With chemiluminescence technique, the effect of various selenium forms and concentrations on ATP centent and GSH-px activity was observed. Compared study with the results of selenium on triple label incorporation of ~(125)Ⅰ-udR. ~(14)C-leu.
     本文采用化学发光技术,测定了不同浓度不同硒化合物对淋巴细胞ATP含量及GSH—PX活性的影响,并对~3H、~(14)C、~(125)I三标记参入DNA、RNA、蛋白质分析法的硒毒理研究结果作了简要的对此分析。
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  “化学发光技术”译为未确定词的双语例句
     Using Al2O3 as an absorbent, through column Chromatography, We determined the content of catecholamine in rabbit plasma with high sensitive organic-coupled chemiluminescence,and a good results have been obtained The linear range is 2×10-11~8×10-9g/mL, the detection limit is 8. 22×10-13/mL and the recovery is 97. 3%.
     本文以二氧化二铝为吸附剂,经柱层析分离后,对家兔血浆中儿茶酚胺类神经递质,用高灵敏的有机偶合化学发光技术进行测定,效果良好,线性范围为2×10-11~8×10-9g/mL,最低检测下限为8.22×10-13g/mL,回收率为97.3%。
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     Methods Highly purified HCV Core,NS3,NS4,NS5 proteins were immobilized on solid matrix by biochip arrayer,the protein chip was used to detect the corresponding antibodies in human serum by chemiluminescent immunoassays.
     方法利用生物芯片技术和化学发光技术将高度纯化的抗原HCV Core、NS3、NS4、NS5以特定微阵列固定在固相载体上,用化学发光免疫法检测抗HCV Core、抗HCV NS3、抗HCV NS4和抗HCV NS5。
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     The measurement of rate constant of reaction O~3(P) + SiH_4 with discharge flow-chemiluminescence technique is presented.
     本文报道了用流动放电-化学发光技术测定O(~3P)和硅烷化学反应速率常数.
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     Methods:To determine the CPn antigen in blood serum of 65 inpatients with AMI and 50 healthy persons in clinic in recent two years with enzyme chemiluminescence method and compare the differences of the data in two groups.
     方法:应用酶化学发光技术检测近2年内65例AMI住院病人和门诊50例健康者的血清CPn抗原,比较两组数据的差异。
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     Chemiluminescence in Human Whole Blood and its Clinical Application
     人全血化学发光技术及其临床应用 Ⅰ.用于白细胞吞噬功能的测定
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  相似匹配句对
     Chemiluminescence Immunoassay
     化学发光免疫技术
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     Technology;
     技术
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     Chemiluminescence
     化学发光
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     TECHNOLOGY
     技术
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     A Study of New Technology on chemiluminescence and
     化学发光和生物发光新技术研究
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  chemiluminescence technique
A chemiluminescence technique was applied to study the effects of their scavenging superoxide radicals
      
The generation of free radicals in the cell was registered using the chemiluminescence technique.
      
The chemiluminescence technique was used to determine the radical scavenging activities of CPRI toward different reactive oxygen species (ROS) including superoxide anion (
      
Before and after induction of ischemia leucocytes were isolated and stimulated with opsonized zymosan, and oxygen radical production was measured using the luminol-dependent chemiluminescence technique.
      
The erythrocyte membrane lipid peroxide content, erythrocyte SOD activities were detected by ultraviolet split-beam spectrophotometry and chemiluminescence technique.
      
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Chemiluminescence occurs when the exothermicity (ΔG) of a chemical reaction is converted into electronic excitation energy of a reaction product, which then emits photons of light (hv). We can describe chem.ilum.i-nescence as a sequence. The heart of the sequence is the chemi-excitation step.The field of chemiluminescence has experienced tremendous growth and witnessed significant advances in the past more ten years. To a large extent, the recent progress toward the understanding of chemiluminescent processes...

Chemiluminescence occurs when the exothermicity (ΔG) of a chemical reaction is converted into electronic excitation energy of a reaction product, which then emits photons of light (hv). We can describe chem.ilum.i-nescence as a sequence. The heart of the sequence is the chemi-excitation step.The field of chemiluminescence has experienced tremendous growth and witnessed significant advances in the past more ten years. To a large extent, the recent progress toward the understanding of chemiluminescent processes can be attributed to achievements in three general areas. The first area of achievement was the discovery and subsequent intensive investigation of the chemiluminescent reaction of 1,2-dioxetanes. The discovery of this reaction, a simple unimolecular rearrangement, has allowed experimentalists to focus on the study of the key step of chemiexcitation. A second area of recent advance has come in the field of electron-transfer chemiluminescence. Electron-transfer chemiluminescence has been investigated extensively as elctro-generated chemiluminescence (ECL). Using ECL technique significant progreas has been made in recent years toward the understanding of chemiexcitation by electron transfer. A third general area of recent progress has been the confirmation of chemically initated electron-exchange luminescence(CIEEL) as a general mechanism for the general chemical formation of light.In this review we have introduced the classification of chemiluminescent organic reactions, examined the general requirements for a reaction to be chemiluminescent, presented the three important generalized mechanisms of chemiluminescence in solution, and finally enumerated some important chemiluminescent systems.In terms of potential-energy surfaces, we recognize two major classes of chemiluminescent organic reactions: ( 1 ) nonadiabatic chemiluminescence, and ( 2 ) adiabatic chemiluminescence. A classification by reaction type has: oxidation, electron transfer, fragmentation, and pericylic rearrangement.Certainly, the most stringent requirement for efficient chemiexcitation is one of energetics, ΔG + ΔG≥ΔE + ΔE' (1)where +++E is the energy of the excited state, ΔE' is a geometry factor. It is clear that spin-orbit coupling factors could play a significant role in determining the efficiency of triplet-state generation (e.g. for the chemilumi-nescence thermolysis of 1,2-dioxetanes).Up to now, three general mechanism have been proposed to explain the chemiluminescence of organic compounds. In the first mechanism, a high-energy reactant molecule undergoes an exergonic reaction, typically a rearrangement or fragmentation, to generate a product molecule in an electronically excited state (scheme 1 ). The second general mechanism for chemical light formation is radical ion annihilation (scheme 2). The third generalized mechanism, for chemiluminescence of organic molecules in solution has been identified recently as chemically initiated electron-exchange luminescence (CIEEL)(scheme 3).Some important chemiluminescent systems are:peroxyoxalates,dioxetanes, dioxetanones, diphenol peroxides, a cyclic secondary peroxyesters and Iu minol. Recently in peroxyoxalates, we have found a new method for preparing aryl peroxyoxalates. The reaction is as follow:2ArOH+(COOH)2+POCl3→(COOAr)2 +HPO3+3HC1 (2).

在过去十多年里,有关有机化合物化学发光的研究增长很快,在三个方面取得了新进展:(1)1,2-二氧杂丁烷体系化学发光的发现和研究,有助于人们了解单分子化学激发作用;(2)开展了电子转移化学发光的研究,用电生化学发光技术,研究电子转移的化学激发过程;(3)确认了化学引发电子变换发光(CIEEL)为一般化学发光机理。 本文将介绍化学发光有机反应的分类,说明化学发光的一般要求,叙述三个化学发光机理以及列出一些重要的化学发光体系。 在过氧化草酸芳酯体系中,我们提出了一种合成草酸芳酯的新方法,其反应如下: 2ArOH+(COOH)_2+POCl_3→(COOAr)_2+HPO_3+3HCl

This paper has more detailedly discussed the basic components and functions of general chemiluminescence instrument. Characteristics of several commercial instruments have been reviewed and compared. The advances in new chemiluminescence techniques, such as chemiluminescence fiberoptic probe, site-specific chemiluminescence probe, chemilum inescence flow injection analysis, chemilum inescence com bined with chromatography, microcomputer application etc. have been also described.

本文详细介绍了一般化学发光仪器的基本组成和作用,评价、比较了几种商品仪器的特点。对化学发光技术的最新进展,如化学发光光导纤维探头、定位化学发光探头与色谱联用,以及与微机相结合也作了介绍。

The measurement of rate constant of reaction O~3(P) + SiH_4 with discharge flow-chemiluminescence technique is presented. The Arrhenius form of the rate constantover temperature range 293--413K is k= (1.05±0.36)×10~(-10)exp[(-3.06±0.10)kcal·mol~(-1)/RT]cm~3·molecule~(-1)·s~(-1)Using transition-state theory, the result was extrapolated to temperature range of 200--2000K. The calculated dependence of the rate constant on temperature with threeparameters isk = 7.67×10~(-19)T~(2.59)exp (-720cal·mol~(-1)/RT)cm~3·molecule~(-1)·s~(-1)....

The measurement of rate constant of reaction O~3(P) + SiH_4 with discharge flow-chemiluminescence technique is presented. The Arrhenius form of the rate constantover temperature range 293--413K is k= (1.05±0.36)×10~(-10)exp[(-3.06±0.10)kcal·mol~(-1)/RT]cm~3·molecule~(-1)·s~(-1)Using transition-state theory, the result was extrapolated to temperature range of 200--2000K. The calculated dependence of the rate constant on temperature with threeparameters isk = 7.67×10~(-19)T~(2.59)exp (-720cal·mol~(-1)/RT)cm~3·molecule~(-1)·s~(-1).

本文报道了用流动放电-化学发光技术测定O(~3P)和硅烷化学反应速率常数.在293—413K范围内,结果为k=(1.05±0.36)×10~(-10)exp[(-3.06±0.10)kcal. mol~(-1) /RT]cm~3·molecule~(-1)·s~(-1)并用过渡态理论将上述实验结果外推到200—2000K范围内.计算结果以三参数公式表示为: k=7.67×10~(-19) T~(2.59) exp(-720cal.mol~(-1)/RT)cm~3·molecule~(-1)·s~(-1).

 
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