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点阵技术
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  lattice technique
     Windows Font Based on Lattice Technique in Billboard Displaying
     Windows环境下字体的点阵技术
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  “点阵技术”译为未确定词的双语例句
     Application of Microarray Technology in research on Neuro-reproductive Toxicities of 1-Bromopropane and 2-Bromopropane
     运用基因微点阵技术研究1-溴丙烷与2-溴丙烷的神经-生殖毒性(英文)
     Robotically spotted DNA microarray technology and its application to profile gene expression(I)
     机械点样DNA微点阵技术及其在基因表达分析上的应用(I)
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     Robotically spotted DNA microarray technology and its application to profile gene expression(II)
     机械点样DNA微点阵技术及其在基因表达分析上的应用(II)
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     cDNA clones were isolated from a cDNA library prepared from swamp eel testies by macroarray. DNA sequence analysis and database search showed that they encode 8 proteins which are highly homologous to 40S ribosomal proteins S4,S9,S16,S17,S20 and 60S riobosomal proteins L7, L18a,L29. Phylogenetic trees (ML) based on ribosomal protein genes from swamp eel and other organisms were reconstructed, which showed that ribosomal protein genes were highly conserved during evolution.
     采用高密点阵技术从黄鳝雄性性腺cDNA文库中获得 8 个克隆,序列分析和 BLAST结果显示它们编码的蛋白质分别与40S核糖体蛋白S4,S9,S16,S17,S20 和 60S核糖体蛋白 L7, L18a,L29 高度同源。 根据黄鳝 RP蛋白序列和其他物种的相应同源序列构建ML系统发生树,显示核糖体蛋白基因在进化中高度保守。
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     Methods After stable expressing NS4B cell line established, the influence of NS4B on cellular gene expression profiling was studied using DNA microarray; the expression of two up-regulated and two down-regulated genes from the result of DNA microarray was analyzed via real-time quantitative RT-PCR; the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was analyzed.
     方法通过建立稳定表达NS4B的细胞系,用微点阵技术研究NS4B对细胞基因表达的影响,实时定量RT-PCR分析微点阵结果中的两个上调基因(DKK1,FYN)和两个下调基因(AKR1C1,v-fos)的表达,对细胞中AKR1C1的活性进行酶学分析。
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     TECHNOLOGY
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     VECTORIZATION TECHNIQUE OF DOT CHIESE CHARACTERS
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     Technology;
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     Interlacing Technique for New Carbon Fiber Lattice Materials
     新型碳纤维点阵复合材料技术研究
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     The Mesh Point Battle
     网格点阵
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In this paper, the hemispherical grating technique, which is effectively used in enhancing quantum efficiency of Si photodetector, is introduced. By using this technique, PIN and RAPD photodetector are fabricated.

本文介绍了提高硅光探测器量子效率的半球形点阵技术。介绍了运用这一技术制作的P-I-N和RAPD硅光探测器。

To investigate the gene expression profiles of five different stages in the formation of esophageal squamous cell carcinoma (normal, dysplasia Ⅰ, dysplasia Ⅱ, carcinoma in situ, squamous cell carcinoma) and to explore the significance of cDNA microarray technology . Extracted total RNA, synthesized cDNA probes and hybrided with cDNA microarray membranes from collect tissue were performed.For randomly selected eight ESTs,RT_PCR was performed to confirm the microarray results with original hybridization and matched...

To investigate the gene expression profiles of five different stages in the formation of esophageal squamous cell carcinoma (normal, dysplasia Ⅰ, dysplasia Ⅱ, carcinoma in situ, squamous cell carcinoma) and to explore the significance of cDNA microarray technology . Extracted total RNA, synthesized cDNA probes and hybrided with cDNA microarray membranes from collect tissue were performed.For randomly selected eight ESTs,RT_PCR was performed to confirm the microarray results with original hybridization and matched tissue samples. The data were acquired with special software and analyzed after normalizing. RT PCR results of 6 ESTs were consistent with hybridization results for these two kinds of samples. Data analysis showed that compared normal with other four stages there were 492,481,473 and 501 genes , whose expression levels varied two folds or more. 386 genes underexpressed in all abnormal stages. But the expression levels of most genes changed little among four unnatural stages. The expression patterns of some known tumor related genes were observed.[ Conclusions] It is demonstrated that dysplasia Ⅰ is an early stage in the formation of esophageal squamous cell carcinoma. Many genes expression show abnormally in the oncogenesis of esophagus, which is involved in cell growth, immunity, DNA repair, and so on. Therefore, cDNA microarray technology will be helpful to find key genes of esophageal squamous cell carcinoma and provide new clues to diagnosis, therapy and prevention of this disease.

[目的]初步了解食管鳞癌形成中5个不同时期 (正常、食管上皮不典型增生Ⅰ级、食管上皮不典型增生Ⅱ级、食管原位鳞癌和食管鳞癌 )的基因表达谱 ,并探讨cDNA微点阵技术在肿瘤研究中的应用价值。[方法]收集上述5种不同病理类型的组织标本 ,提取总RNA ,逆转录合成cDNA探针 ,分别与cDNA微点阵膜杂交。随机挑选8个EST片段 ,分别以杂交样品和配对组织标本总RNA为模板完成RT PCR以验证杂交结果的准确性。用专用软件分析得到各位点光密度值 ,将此数值标准化后进一步比较分析。[结果]对于杂交样品和配对组织标本 ,都是6个EST片段的RT PCR结果与其杂交结果一致。数据分析显示 ,正常时期和其他各期相比分别有492,481 ,473和501个基因的表达改变了2倍及2倍以上。其中386个基因在4个异常时期均表达下调。而其他各期之间相比 ,大多数基因的表达无明显改变。还观察了一些已知的肿瘤相关基因在5个不同时期的表达状况。[结论]本实验再一次证实食管上皮不典型增生Ⅰ级是食管鳞癌形成的一个早期阶段。利用cDNA微点阵技术将有望发现导致食管鳞癌形成的关键基因 ,为临床早期诊断、治疗和预防提供...

[目的]初步了解食管鳞癌形成中5个不同时期 (正常、食管上皮不典型增生Ⅰ级、食管上皮不典型增生Ⅱ级、食管原位鳞癌和食管鳞癌 )的基因表达谱 ,并探讨cDNA微点阵技术在肿瘤研究中的应用价值。[方法]收集上述5种不同病理类型的组织标本 ,提取总RNA ,逆转录合成cDNA探针 ,分别与cDNA微点阵膜杂交。随机挑选8个EST片段 ,分别以杂交样品和配对组织标本总RNA为模板完成RT PCR以验证杂交结果的准确性。用专用软件分析得到各位点光密度值 ,将此数值标准化后进一步比较分析。[结果]对于杂交样品和配对组织标本 ,都是6个EST片段的RT PCR结果与其杂交结果一致。数据分析显示 ,正常时期和其他各期相比分别有492,481 ,473和501个基因的表达改变了2倍及2倍以上。其中386个基因在4个异常时期均表达下调。而其他各期之间相比 ,大多数基因的表达无明显改变。还观察了一些已知的肿瘤相关基因在5个不同时期的表达状况。[结论]本实验再一次证实食管上皮不典型增生Ⅰ级是食管鳞癌形成的一个早期阶段。利用cDNA微点阵技术将有望发现导致食管鳞癌形成的关键基因 ,为临床早期诊断、治疗和预防提供新的线索。

DNA microarray or DNA chip technology has been explored for profiling gene expression, comparing genomic contents, analyzing DNA sequence variations, and genotyping, etc . With the increasing availability of complete microbial genomes, DNA microarray technology is destined to become a common tool in many areas of microbiological research, including microbial physiology, pathogenesis, epidemiology, ecology, phylogeny, pathway engineering, and fermentation optimization.In this article,the current advances in...

DNA microarray or DNA chip technology has been explored for profiling gene expression, comparing genomic contents, analyzing DNA sequence variations, and genotyping, etc . With the increasing availability of complete microbial genomes, DNA microarray technology is destined to become a common tool in many areas of microbiological research, including microbial physiology, pathogenesis, epidemiology, ecology, phylogeny, pathway engineering, and fermentation optimization.In this article,the current advances in microbiological research based on DNA microarray technology are reviewed.

DNA微点阵 (DNA芯片 )技术已经被广泛应用于基因表达分析、比较基因组学、DNA序列变异分析和基因分型等多方面的研究。随着越来越多的微生物种类完成全基因组测序 ,在微生物生理、致病机制、流行病学、生态学、系统发育学、途径工程、发酵最优化等研究领域中 ,DNA微点阵技术必将成为一个常用的研究手段

 
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