In the study, embryo bud of Impatiens balsamina L was cultured on hormone-free MS medium for promoting, MS medium with 6-BA 3 mg/L+NAA 0.5 mg/L for shoot and root formation, and MS medium containing 6-BA 3 mg/L+NAA 0.5 mg/L for flowering respectively. During about 60 days, plant regeneration and flowering of Impatiens baisamina L was finished in vitro.
The percentage of embryos forming shoots and whole plant were increased to 83. 3% and 50.0% by adding 0.1 mg/1 NAA, but high concentration NAA (more 0.1 mg/1) inhibited the germination of embryo germination and plant regeneration.
This paper deals with studying on the tissue culture of rehmannia glutinosa, includinginduction of callus and formation of regenerated plantlet. Results showed: (1) 6 - BA1mg/1+ NAA0. 1mg/L combinations were advantageous to leaf callus inclution and 2, 4 - D2mg/L+NAA 2mg/L combinations were due to root-stem callus induction in callus induction;
Regenerated plantlet, hypocotyl segment, cotyledon are more sensible than embryogenic callus, which maybe indicate that GS2, the chief form of GS in chloroplast, is more sensible for PPT than GS1, the dominating form of GS existing in callus.
3. The survival rate of regeneration plant transferring reaches 80.95%, it is best to choose "lakali" as transfer substance in earlier stage, then move into mixed soil:pearlite = 1:1 for plants' growing.
Cytogenetic analyses were performed to determine the expression of this in vitro chromosomal variability in corresponding regenerated plant tissues.
Eight embryoids and three regenerated plants examined had 28 chromosomes identical to the original hybrid plants, while one regenerated plant was found to be a mixploid composed of cells with 28 and 56 chromosomes.
It was concluded that the occurrence of variation after vegetative propagation was due to somatic segregation of chimeras resulting from gene mutations or chromosome structural rearrangements in only part of the regenerated plant.
Testcross kernels from one regenerated plant (LT20) segregated for wild-type and high free threonine concentration in a 1∶1 ratio consistent with a single dominant gene for high free threonine.
The hybrid nature of the regenerated plant was confirmed morphologically and cytogenetically.
Polyamines increased mitotic index of cells in root tips of regenerated plantlet cultured on medium containing 0.001?μM Put, Spd, or Spm, but did not increase mitotic index in tissues of needle tips of the same plantlets.