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胚胎拯救
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  embryo rescue
     Two monosomic alien addition lines 02-17 and 02-39 (2n=15, 14C+1H) were identified among 52 plants derived from embryo rescue of an Cucumis allotriploid x cucumber.
     将甜瓜属异源三倍体与栽培黄瓜‘北京截头’杂交,经胚胎拯救获得了两个2n:15(14C+1H)的单体异附加系,编号分别为02-17和02-39。
短句来源
     The 1 848 florets of Oryza sativa were pollinated with Oryza australiensis and 48 immature seeds were obtained were cultured for embryo rescue. 11 of the 45 seeds could germinate to be F1 hybrid plants. They were all male sterile,the rate of crossability was 0.60%and the rate of germination was 31.1%.
     用Oryza australiensis花粉对Oryza sativa共1848朵小花进行杂交授粉,并对杂交获得的48粒F1代未成熟种子进行了胚胎拯救,有11粒F1种子发芽并发育成植株,全部不育,其杂交率为0.60%,萌芽率为31.1%。
短句来源
     The 1,848 florets of Oryza sativa were pollinated with Oryza australiensis and 48 immature seeds were obtained were cultured for embryo rescue. 11 of the 45 seeds could germinate to be F1 hybrid plants. They were all male sterile, the rate of crossability was 0.60% and the rate of germination was 31.1%.
     用Oryzaaustraliensis花粉对Oryzasativa共1848朵小花进行杂交授粉,并对杂交获得的48粒F1代未成熟种子进行了胚胎拯救,有11粒F1种子发芽并发育成植株,全部不育,其杂交率为0.60%,萌芽率为31.1%。
短句来源
     The ratio of fruit with embryo was 60%-67% in the crosses between C. ×hytivus and cucumber cultigens. Each fruit had about 10-20 embryos, which could survive and develop into plantlets through embryo rescue.
     结果表明,各种杂交组合的坐果率达到83%~100%,其中以异源四倍体×栽培黄瓜获得含有胚的果实比例较高,为60%~67%,平均每个果实中约有10~20个胚,通过胚胎拯救,成活率接近100%,染色体计数为26条,为异源三倍体。
短句来源
     A system for embryo rescue has been developed to overcome prefertilization and postfertilization barriers,and new lily cultivars and hybrids have been obtained.
     利用胚胎拯救技术克服了百合杂交前后障碍获得百合新品种 ;
短句来源
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  “胚胎拯救”译为未确定词的双语例句
     2. An allotriploid derived from a amphidiploid X diploid mating in Cucumis : production, micropropagation and verificationA fully fertile interspecific hybrid (Cucumis hytivus Chen and Kirkbride, 2n = 4x = 38) between Cucumis hystrix Chakr.
     2.异源三倍体的培育及离体繁殖研究 通过胚胎拯救和体细胞突变加倍获得的完全可育的黄瓜属双二倍体种(Cucumis hytivus Chen and Kirkbride,2n=4x=38)是转移野生种有用性状的桥梁。
短句来源
     The first repeatable interspecific hybridization between cucumber (C.sativus L., 2n=14) and a wild Cucumis species,C.
     采用胚胎拯救方法 ,首次成功实现了栽培黄瓜 (Cucumis sativus L.,2 n=1 4)与同属野生种 C.
短句来源
     Only one seed could germinate to be F1 hybrid plant. The plant set one F2 seed. The rate of crossability ranged from 0 to 0.46%, the rate of germination ranged from 0 to 50% and the rate of seed set of the F1 hybrid plant was 0.024%(1/4,211).
     用Oryzameridionalis花粉对Oryzasativa共2419朵小花进行杂交授粉,并对杂交获得的18粒F1代未成熟种子进行了胚胎拯救,最终仅有一粒F1种子发芽并发育成植株,并获得一粒F2代种子,其杂交率为0.46%,萌芽率为50%,F1代杂种的结实率为0.024%;
短句来源
     Apical and axillary buds of hybrids FI, which was obtained through embryo rescuing, were used as explants to establish the micropropagation system.
     利用胚胎拯救获得的杂种F_1无菌苗的茎尖或腋芽建立了杂种F_1的快速繁殖体系。
短句来源
     Only one seed cou ld germinate to be F1 hybrid plant. The plant set one F2 seed. The rate of cross ability ranged from 0 to 0.46%,the rate of germination ranged from 0 to 50%an d the rate of seed set of the F1 hybrid plant was 0.024%.
     本试验用Oryza meridionalis花粉对Oryza sativa共2419朵小花进行杂交授粉,并对杂交获得的18粒F1代未成熟种子进行胚胎拯救,最终仅有一粒F1种子发芽并发育成植株,并获得一粒F2代种子,其杂交率为0.46%,萌芽率为50%,F1代杂种的结实率为0.024%;
短句来源
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  相似匹配句对
     SALVATION
     拯救
短句来源
     The Path to Salvation
     拯救之道
短句来源
     Study on the embryonic development of Paralichthys olivaceus
     牙鲆的胚胎发育
短句来源
     (3) the stage of embryonic development;
     3)胚胎发育期。
短句来源
     A system for embryo rescue has been developed to overcome prefertilization and postfertilization barriers,and new lily cultivars and hybrids have been obtained.
     利用胚胎拯救技术克服了百合杂交前后障碍获得百合新品种 ;
短句来源
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  embryo rescue
Agar-solidified nutrient media modified from that developed previously for tissue and cell cultures of red clover (2 n = 14) were used for embryo rescue.
      
All wheat-Agropyron hybrids were obtained by embryo rescue technique.
      
corymbosum zygotes using embryo rescue techniques, even when embryos were cultured at proembryonic stages.
      
New interspecific hybrids between alfalfa (Medicago sativa L.) and several perennial Medicago species were obtained by embryo rescue techniques.
      
rupestris F1 and first backcross generation embryos demonstrated the optimum concentration of 12.5 mM NH4+ for successful embryo rescue; ammonium salt formulation (whether chloride, nitrate or sulfate) was not critical.
      
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The first repeatable interspecific hybridization between cucumber (C.sativus L., 2n=14) and a wild Cucumis species,C.hystrix Chakr.(2n=24) was successfully made through embryo rescue.The hybrid plants were morphologically uniform.While the multiple branching habit,densely brown hairs (especially on corolla and pistil),orange-yellow corolla,and ovate fruit of F 1 hybrid plants were similar to that of the C.hystrix parent,appearance of the first pistillate flower was more similar to that of C.sativus parent.The...

The first repeatable interspecific hybridization between cucumber (C.sativus L., 2n=14) and a wild Cucumis species,C.hystrix Chakr.(2n=24) was successfully made through embryo rescue.The hybrid plants were morphologically uniform.While the multiple branching habit,densely brown hairs (especially on corolla and pistil),orange-yellow corolla,and ovate fruit of F 1 hybrid plants were similar to that of the C.hystrix parent,appearance of the first pistillate flower was more similar to that of C.sativus parent.The diameter and internode length of stem,shape and size of leaves and flowers were intermediate when compared to that of the parents.Self-pollination and backcrossing of the F 1 hybrid plants to either parent indicated that the original hybrid was both male- and female-sterile,probably due to meiotic abnormalities caused by lack of homology and the odd chromosome number 2n=19 (including 7 from cucumber and 12 from C.hystrix).The chromosome number in the hybrid was doubled with somaclonal mutation to restore the fertility.Pollen grains were released from the synthetic amphidiploid and fruits set with viable seeds on the fertility-restored plants.The results from flow cytometry indicated that,on average,7.3% of the regenerants with unique morphology were chromosome-doubled F 1 hybrids.The 2C DNA content of the original F 1 hybrid was 1.17 pg,and the 4C DNA content was 2.35 pg.This new species was preliminarily characterized in growth and development,nutrition value,and disease resistance.The results suggested it a possible place to be a new Cucumis crop in future agriculture.Two types of fruits produced in our first season of crosses included a potential pickling variety that produces multiple uniform fruits at a single node with the 1∶3 length/width ratio for once over harvest.A second cross produces long slender fruits,nearly seedless with the ability to grow in partially shaded conditions,which may be the suitable varieties for greenhouse production.Results from nutrition analysis indicated that the synthetic species has higher protein (0.78%) and mineral (0.35%) content compared to the normal pickling cucumber (0.62% and 0.27%,respectively).A screen for root-knot nematode resistance revealed a high level of resistance existed in C.hystrix,and the resistance was partially transmitted to the F 1 and the chromosome-doubled F 1 when the reciprocal hybrid was made.

采用胚胎拯救方法 ,首次成功实现了栽培黄瓜 (Cucumis sativus L.,2 n=1 4)与同属野生种 C.hystrix Chakr.(2 n=2 4 )间可重复的种间杂交。杂交 F1植株形态一致。其中多分枝、密棕茸毛 (尤其是在花瓣和雌蕊上 )、桔黄色花冠及卵圆形果实这些特征与亲本 C.hystrix相似 ,而第一雌花节位则与亲本黄瓜 (C.sativus)相似。其它性状如株径、节长、叶和花的形状和大小等都介于双亲之间而呈中间型。将杂种 F1植株自交并与两亲本进行回交 ,结果表明 F1杂种的雄蕊和雌蕊都是不育的。这可能是由于杂种染色体数目为奇数 (2 n=1 9,其中 7条来自黄瓜 ,1 2条来自野生黄瓜 ) ,缺乏同源性而导致减数分裂不正常。利用体细胞无性系突变方法 ,对杂种的染色体数进行了加倍。流动细胞计量仪测定表明 ,加倍的 F1植株 (双二倍体 )占再生植株的 7.3 %,形态一致 ,其 DNA含量为 2 .3 5 pg,而 F1(二倍体 )的含量为 1 .1 7pg。新合成的双二倍体植株能释放花粉 ,并且能形成含种子的果实。对生长和发育、营养价值及抗性等方面初步研究表明 ,...

采用胚胎拯救方法 ,首次成功实现了栽培黄瓜 (Cucumis sativus L.,2 n=1 4)与同属野生种 C.hystrix Chakr.(2 n=2 4 )间可重复的种间杂交。杂交 F1植株形态一致。其中多分枝、密棕茸毛 (尤其是在花瓣和雌蕊上 )、桔黄色花冠及卵圆形果实这些特征与亲本 C.hystrix相似 ,而第一雌花节位则与亲本黄瓜 (C.sativus)相似。其它性状如株径、节长、叶和花的形状和大小等都介于双亲之间而呈中间型。将杂种 F1植株自交并与两亲本进行回交 ,结果表明 F1杂种的雄蕊和雌蕊都是不育的。这可能是由于杂种染色体数目为奇数 (2 n=1 9,其中 7条来自黄瓜 ,1 2条来自野生黄瓜 ) ,缺乏同源性而导致减数分裂不正常。利用体细胞无性系突变方法 ,对杂种的染色体数进行了加倍。流动细胞计量仪测定表明 ,加倍的 F1植株 (双二倍体 )占再生植株的 7.3 %,形态一致 ,其 DNA含量为 2 .3 5 pg,而 F1(二倍体 )的含量为 1 .1 7pg。新合成的双二倍体植株能释放花粉 ,并且能形成含种子的果实。对生长和发育、营养价值及抗性等方面初步研究表明 ,这一新物种可望成为一新型作物种 ,在未来农业中占有一席之地。通过不同杂交可形成两种类型的果实 :一种为腌渍类型 ,该株系每株可着生 3 0多个大约 1 0 cm长的果实 ,可一次性采收 ;另一种为耐弱光类

The recent applications of biotechnology in lily ( Lilium spp.) were reviewed. A successful and efficient large scale propagation systems and a protocol to eliminate virus of lily have been established by means of tissue culture and rapid propagation. A system for embryo rescue has been developed to overcome prefertilization and postfertilization barriers,and new lily cultivars and hybrids have been obtained. Protein markers and DNA markers were applied widely in lily. Several gene transfer procedures...

The recent applications of biotechnology in lily ( Lilium spp.) were reviewed. A successful and efficient large scale propagation systems and a protocol to eliminate virus of lily have been established by means of tissue culture and rapid propagation. A system for embryo rescue has been developed to overcome prefertilization and postfertilization barriers,and new lily cultivars and hybrids have been obtained. Protein markers and DNA markers were applied widely in lily. Several gene transfer procedures have been initiated,and production of transgenic lily by micro projectile bombardment has recently been reported.

对生物技术在百合 (Liliumspp .)上的应用研究进展进行了综述 .生物技术在百合上的应用主要包括以下几个方面 :利用植物离体培养技术成功建立了百合快速繁殖体系和脱毒苗生产技术程序 ;利用胚胎拯救技术克服了百合杂交前后障碍获得百合新品种 ;蛋白质分子标记、DNA分子标记在百合的初步应用 ;通过农杆菌介导法、基因枪法、电激法等进行了百合的遗传转化 ,并通过基因枪法成功地获得了百合转基因的植株 .

Two monosomic alien addition lines 02-17 and 02-39 (2n=15, 14C+1H) were identified among 52 plants derived from embryo rescue of an Cucumis allotriploid x cucumber. Different from their allotriploid parent, the plants have white spine, and the shape of leaves in 02-17 and 02-39 was hastate and palmate, respectively. In pollen mother cells (PMCs) of 02-17 and 02-39, 7 bivalents and 1 univalent were observed at diakinesis and metaphase Ⅰ. Low frequency (about 4% - 5%) of trivalents has also been observed at metaphase...

Two monosomic alien addition lines 02-17 and 02-39 (2n=15, 14C+1H) were identified among 52 plants derived from embryo rescue of an Cucumis allotriploid x cucumber. Different from their allotriploid parent, the plants have white spine, and the shape of leaves in 02-17 and 02-39 was hastate and palmate, respectively. In pollen mother cells (PMCs) of 02-17 and 02-39, 7 bivalents and 1 univalent were observed at diakinesis and metaphase Ⅰ. Low frequency (about 4% - 5%) of trivalents has also been observed at metaphase Ⅱ . At metaphase Ⅱ , 8 chromosomes went to one pole while 7 to another. RAPD analysis indicated these two plants were added with one alien chromosome from C. hystrix .

将甜瓜属异源三倍体与栽培黄瓜‘北京截头’杂交,经胚胎拯救获得了两个2n:15(14C+1H)的单体异附加系,编号分别为02-17和02-39。02-17植株叶形为戟形,02-39为深陷的掌状形,并且果刺均为白色,不同于异源三倍体的掌状心脏形和黑刺,果形比异源三倍体黄瓜长而更接近普通黄瓜。细胞学研究发现,单体异附加系在终变期和中期I染色体构型主要是由7个二价体和1个单价体构成,出现三价体的频率为4%-5%,中期Ⅱ不均等分裂形成不同的两极,一极染色体为8条,另一极为7条。RAPD分析证明它们分别附加了野生种Cucumis hystrix的染色体。

 
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