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旋转相关时间
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  rotational correlation time
    Results: The hs/hw ratio of 2 and 6 h postburn groups increased significantly postburn compared with that of shamburn group, and so did the rotational correlation time(τc) in 1 to 12 h postburn groups.
    结果:伤后2和6h膜蛋白hs/hw显著增大,伤后1~12h膜蛋白旋转相关时间τc显著增大。
短句来源
    The electron paramagnetic resonance (EPR) was used to characterize the protein conformation and movement described by the ratio of the strong solidity and weak solidity (h S/h W) and rotational correlation time (τ c) of membrane proteins] of spin labelled sulfhydryl ( SH) group in myocytes membrane.
    应用电子顺磁共振波谱测定心肌细胞膜蛋白巯基强 /弱固定相峰高比值 (h S/ h W)和膜蛋白旋转相关时间 (τc)的动态变化 ,同时直接测定心肌组织中氧自由基水平。
短句来源
    The electron paramagnetic resonance(EPR) was used to characterize the protein conformation and movement [described by the ratio of the strong solidity and weak solidity(hs/hw)and rotational correlation time(τ_c) of membrane proteins] of spin-labeled sulfhydryl(-SH) group in myocytes membrane.
    应用电子顺磁共振波谱测定心肌细胞膜蛋白巯基强/弱固定相峰高比值(hs/hw)和膜蛋白旋转相关时间(τc)的动态变化,同时直接测定心肌组织中氧自由基水平。
短句来源
  “旋转相关时间”译为未确定词的双语例句
    Decreased LVSP, dp/dt max and dp/dt min were found during reperfusion period in bothIR group and IPC group as compared to that of Control group (P<0.05). However, LVSP,dp/dt max and dp/dt min in IPC group were significantly higher than that in IR group(P<0.05). Then there were obvious recovery tendency at 90 min after reperfusion in both IRgroup and IPC group.
    1 采用电子顺磁共振法测定心肌细胞膜蛋白巯基强/弱固定相峰高比值 hS / hW和膜蛋白旋转相关时间 c 的动态变化 IR 组和 IPC 组 hS/ hW和 在 ACC 期间明c显升高 并于心肌RP早期进一步上升 但IPC组hS/ hW和 均明显低于IR组 P <0.05c随后 IR 组和 IPC 组 hS/ hW和 均有不同程度的回落c
短句来源
    The results showed that,in delayed fluid resuscitation group after burn shock, membrane crosslinking protein appeared in erythrocytes,the function of band 3 protein was damaged,the recovery time after fluorescence photobleaching of membrane protein was obviously prolonged,and the ratio of peak heights of strong to weak immobilization was increased,especially in the six-hour delayed resuscitation group,suggesting that the ischemia-reperfusion injury was more serious during the early shock stage.
    结果显示:烧伤休克延迟复苏后红细胞膜蛋白组份中出现高分子量交联蛋白(HMP),膜带3蛋白功能受损,膜蛋白侧向运动显著减弱,马来酰亚胺标记的大鼠红细胞膜在延迟复苏后其电子自旋共振波谱中强弱固定化作用谱的峰值比增大,旋转相关时间延长,膜蛋白的构象变化。
短句来源
    Results:During aortic cross clamping(ACC) and reperfusion,both order parameter(S) and rotation correlation time(τ c) in all animals increased significantly,and reached peak value at 1hr after reperfusion,and then fell.
    结果:升主动脉阻断期间和再灌注早期,各组各时间点序参数(S)和旋转相关时间(τc)均明显增大,至再灌注60分钟达峰值后开始恢复;
短句来源
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  rotational correlation time
A share of membrane-bound spin probe was different for chloroplasts and subchloroplast fragments, as well as its rotational correlation time and apparent enthalpy and entropy activation of nitroxide rotational motion.
      
However, the rotational correlation time τ for spin-labeled Bs and its complex with Bn in solution corresponded precisely to their molecular weights.
      
The rotational correlation time of fulleropyrrolineoxyl was found to be about eight times longer than that of 4-oxy-2,2,6,6-tetramethylpiperidine-1-oxyl.
      
The rotational correlation time, τc, of the spin label tempamine has been used for a comparative study of internal microviscosity in normal and in homozygous β-thalassemic erythrocytes.
      
The effective rotational correlation time, σc, of the spin label 5-SASL results about one order of magnitude higher in the presence of 60 mitogen molecules/vesicle.
      
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The dynamic properties of membrane protein are closely correlated to the membrane functions in erythrocytes. The anion transport function of band 3 protein,the constituents and the translational diffusion of membrane protein was detected in 25% TBSA burn rats. The changes in membrane protein SH were investigated by using the spin label of 3-maleimide-proxyl.The results showed that, in delayed fluid resuscitation group after burn shock,membrane crosslinking protein appeared in erythrocytes,the function of band...

The dynamic properties of membrane protein are closely correlated to the membrane functions in erythrocytes. The anion transport function of band 3 protein,the constituents and the translational diffusion of membrane protein was detected in 25% TBSA burn rats. The changes in membrane protein SH were investigated by using the spin label of 3-maleimide-proxyl.The results showed that, in delayed fluid resuscitation group after burn shock,membrane crosslinking protein appeared in erythrocytes,the function of band 3 protein was damaged,the recovery time after fluorescence photobleaching of membrane protein was obviously prolonged,and the ratio of peak heights of strong to weak immobilization was increased,especially in the six-hour delayed resuscitation group,suggesting that the ischemia-reperfusion injury was more serious during the early shock stage. The authors believe that the results may represent the mechanism of damage biological membrane after burns. The alterations of membrane properties caused by free radicals play an important role in erythrocyte injury.

我们采用25%TBSAⅢ度烧伤大鼠模型,考察了烧伤休克延迟复苏后红细胞膜蛋白结构、功能和运动等特性的改变。结果显示:烧伤休克延迟复苏后红细胞膜蛋白组份中出现高分子量交联蛋白(HMP),膜带3蛋白功能受损,膜蛋白侧向运动显著减弱,马来酰亚胺标记的大鼠红细胞膜在延迟复苏后其电子自旋共振波谱中强弱固定化作用谱的峰值比增大,旋转相关时间延长,膜蛋白的构象变化。实验证明:烧伤休克延迟复苏所造成的非典型的缺血──再灌注损伤通过膜蛋白表层巯基的氧化以及膜脂质过氧化作用引起了膜蛋白的交联,既改变了膜蛋白的构象,又削弱了膜蛋白的分子运动,进而可导致膜功能的异常。

The dynamic properties of membrane protein are closely correlated to the membrane functions in erythrocytes.The anion transport function of band 3 protein,the constituents and the translational diffusion of membrane protein was detected in 25% TBSA burn rats.The changes in membrane protein SH were investigated by using the spin label of 3-maleimide-proxyl. The results showed that,in delayed fluid resuscitation group after burn shock, membrane crosslinking protein appeared in erythrocytes,the function of band...

The dynamic properties of membrane protein are closely correlated to the membrane functions in erythrocytes.The anion transport function of band 3 protein,the constituents and the translational diffusion of membrane protein was detected in 25% TBSA burn rats.The changes in membrane protein SH were investigated by using the spin label of 3-maleimide-proxyl. The results showed that,in delayed fluid resuscitation group after burn shock, membrane crosslinking protein appeared in erythrocytes,the function of band 3 protein was damaged,the recovery time after fluorescence photobleaching of membrane protein was obviously prolonged,and the ratio of peak heights of strong to weak immobilization was increased,especially in the six-hour delayed resuscitation group,suggesting that the ischemia-reperfusion injury was more serious during the early shock stage.The authors believe that the results may represent the mechanism of damage biological membrane af- ter burns.The alterations of membrane properties caused by free radicals play an impor- tant role in erythrocyte injury.

我们采用25%TBSA Ⅲ度烧伤大鼠模型。考察了烧伤休克延迟复苏后红细胞膜蛋白结构、功能和运动等特性的改变。结果显示:烧伤休克延迟复苏后红细胞膜蛋白组份中出现高分子量交联蛋白(HMP),膜带3蛋白功能受损,膜蛋白侧向运动显著减弱,马来酰亚胺标记的大鼠红细胞膜在延迟复苏后其电子自旋共振波谱中强弱固定化作用谱的峰值比增大,旋转相关时间延长,膜蛋白的构象变化。实验证明:烧伤休克延迟复苏所造成的非典型的缺血——再灌注损伤通过膜蛋白表层巯基的氧化以及膜脂质过氧化作用引起了膜蛋白的交联,既改变了膜蛋白的构象,又削弱了膜蛋白的分子运动,进而可导致膜功能的异常。

Objective: To demonstrate the mechanism of early cardiac myocyte membrane damage at molecular level in severely burned rats. Methods: SD rats were randomly divided into shamburn and burn groups. A 30%TBSA burn was created in burn group. Hearts were obtained at different time postburn. Cardiac myocytes isolated by collagenase using Langendoff perfusion apparatus were spin labeled with 3maleidoproxyl. The hs/hw ratio and rotational correlation time were measured according to the spin labelingESR spectra....

Objective: To demonstrate the mechanism of early cardiac myocyte membrane damage at molecular level in severely burned rats. Methods: SD rats were randomly divided into shamburn and burn groups. A 30%TBSA burn was created in burn group. Hearts were obtained at different time postburn. Cardiac myocytes isolated by collagenase using Langendoff perfusion apparatus were spin labeled with 3maleidoproxyl. The hs/hw ratio and rotational correlation time were measured according to the spin labelingESR spectra. Results: The hs/hw ratio of 2 and 6 h postburn groups increased significantly postburn compared with that of shamburn group, and so did the rotational correlation time(τc) in 1 to 12 h postburn groups. Conclusion: There are structural and functional changes in cardiac myocyte membrane after burn injury. They are characterized by increase of hs/hw ratio and rotational correlation time. They could affect the normal performance of cardiac function.

目的:研究严重烧伤大鼠早期心肌细胞膜蛋白结构和运动的变化。方法:用3-甲基马来酰亚胺氮氧自由基(3MP)标记膜蛋白巯基,利用自旋标记-顺磁共振技术,观察膜蛋白旋转相关时间(τc)及强固定化与弱固定化组分谱线高度比值(hs/hw)的变化。结果:伤后2和6h膜蛋白hs/hw显著增大,伤后1~12h膜蛋白旋转相关时间τc显著增大。结论:严重烧伤后早期即出现心肌细胞膜蛋白结构和功能的变化,表现为膜蛋白构象改变,膜蛋白运动受限。这些变化可以直接影响细胞膜蛋白的功能,从而导致心肌细胞功能的变化

 
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