The separation and purification of lysozyme from 150mg protein mixture on TSK GelCM - 5PW, an analytical cation exchange column (Φ7. 5 × 75mm), by overloading elution mode of nonlinear chromatography was reported in this paper.
Retention behavior in nonlinear chromatography—determination of isotherms on stationary phases of GDX-series and its application to qualitative analysis
Displacement chromatography is a kind of non-linear chromatography technology, which is fit to be used for preparative separation as well as direct separation of dilute biologic or trace sample.
In chapter five, artificial neural network (ANN) was applied to the simultaneous optimization of the concentration of organic modifier and the nonlinear chromatographic parameter-pH value of mobile phase.
Using this model, the non-linear retention behaviors of different proteins under the gradient elution in high performance hydrophobic interaction chromatographic column have been predicted.
A method has been established for predicting the retention time of bio-molecules under gradient elution in reversed-phase high performance liquid chromatography by modeling chromatographic behaviors with non-linear differential equations.
The types and features of concentration wave of the chromatographic process in which the mobile phase was emulsion were studied theoretically by the method of characteristics and the concept of multi mobile phase chromatography.
As to the principles of the combined flooding and the movement rules of chemicals, the research advanced multiphase multicomponent chromatographic model and simulated the process of surfactant-polymer flooding.
With use of the theory of nonlinear chromatography, the peak properties of a MIP column, including the retention and peak broadening and tailing, can be well interpreted.
This review leads to the conclusion that nonlinear chromatography theory is useful for characterizing chromatographic features of MIP columns, since a MIP is essentially an affinity-based chromatographic stationary phase.
The possibilities and domain of applicability of the ideal chromatography model for calculating peak profiles in non-linear chromatography are analyzed.
Shifts of chromatographic peak maxima and centres of gravity have been investigated for different amounts of propane injected on to a chromatographic column in ideal, non-linear chromatography.
To predict complex behavior in protein displacement systems of immobilized metal ion affinity chromatography (IMAC), numerical simulation of non-linear chromatography was developed and compared with the ideal solution of the model.