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融合抗原
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  fusion antigen
     CONSTRUCTION AND IDENTIFICATION OF EUKARYOTIC VECTOR EXPRESSING MYCOBACTERIUM TUBERCULOSIS FUSION ANTIGEN AG85B-ESAT6
     结核分枝杆菌融合抗原Ag85B-ESAT6真核表达载体的构建及鉴定
短句来源
     Enhancement of Cellular Immune Responses to Hepatitis B Virus Fusion Antigen DNA Vaccine in Mice by Murine IL-12
     IL-12增强HBV融合抗原DNA疫苗在小鼠诱导的细胞免疫应答
短句来源
     Immunogenicity of a DNA vaccine coexpressing hepatitis B virus surface-core fusion antigen and IL-12.
     乙型肝炎病毒表面—核心融合抗原和白细胞介素—12共表达DNA疫苗的免疫原性
短句来源
     To construct eukaryotic expression vector expressing Mycobacterium tuberculosis fusion antigen Ag85B-ESAT6,the genes respectively encoding Ag85B and ESAT6 protein were amplified by polymerase chain reaction(PCR) based on genome of MTB H37Rv strain. Then by gene SOEing method,fusion gene encoding Ag85B-ESAT6 protein was linked with the linker(GGIGIAPG).
     以结核分枝杆菌H37Rv株的基因组作为模板,将Ag85B和ESAT6编码基因进行PCR扩增,然后采用基因剪接重叠扩增PCR法(gene SOEing)将其通过疏水甘氨酸接头(GGIGIAPG)连接融合,定向克隆至质粒Pvax1中,构建结核分枝杆菌Ag85B-ESAT6融合抗原的真核表达质粒Pvax1/AE.
短句来源
     Immune Responses Elicited by Oral DNA Vaccine of Hepatitis C Virus Fusion Antigen in Mice
     口服型HCV融合抗原DNA疫苗在小鼠诱导免疫应答
短句来源
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  chimeric antigen
     RESULTS: The negative result and the positive result of proteinchips of chimeric antigen accorded with ELISA were 93.8 % and 97.1%, respectively.
     结果:蛋白芯片的融合抗原检测与ELISA法相比,阴性符合率为93.8%,阳性符合率为97.1%.
短句来源
     Methods The dominant epitopes of Treponema pallidum were analyzed and picked out by using computer software. The recombinant multi-epitope chimeric antigen(rTpN15-TpN17-TpN47) expression vector of Treponema Pallidum was constructed,and transformed into E. coli BL21(DE3).
     方法通过计算机软件分析选择梅毒螺旋体优势抗原表位,用聚合酶链反应(PCR)扩增优势表位基因,构建了梅毒螺旋体多优势表位嵌合抗原(rTpN15-TpN17-TpN47)表达载体,转化宿主菌BL21(DE3)进行表达,亲和层析柱法纯化获得高纯度融合抗原,并用其建立检测梅毒抗体的DAS-EIA。
短句来源
     Previous studies in our lab has generated an chimeric antigen named PfCP-2 which is consist of two Plasmodium falciparum erythrocytic stage antigens, AMA-1(III) and MSP1-19. Extremely high level expression of the gene was achieved in Pichia pastoris.
     我们实验室已构建了由AMA-1(III)和MSP1-19组成的恶性疟原虫红内期融合抗原PfCP-2,并在毕氏酵母中得到了高水平表达。
短句来源
     The recombinant multi-epitope chimeric antigen(rTpN15-TpN17-TpN47)expression vector of Treponema Pallidum was constructed,and transformed into E. coli BL21(DE3). Then,the antigen was highly expressed,purified and used for the development of DAS-EIA consequently.
     方法通过计算机软件分析选择梅毒螺旋体优势抗原表位,用聚合酶链式反应(PCR)扩增优势表位基因,构建了梅毒螺旋体多优势表位嵌合抗原(rTpN15-TpN17-TpN47)表达载体,转化宿主菌BL21(DE3)进行表达,亲和层析柱法纯化获得高纯度融合抗原,并用其建立检测梅毒抗体的DAS-EIA。
短句来源
     Among the 294 positive samples, 292 positive samples and 2 negative samples were detected by chimeric antigen on the protein-chip and 288 positive samples, 2 negative samples, 4 indefinite samples were detected by the 4 segments of HCV antigens on the protein-chip.
     阳性标本用蛋白质芯片法检测 ,融合抗原 2 92份显示阳性结果、 2份阴性结果 ,根据蛋白质芯片的核心抗原 ,以及NS3 ,NS4,NS5分片段抗原综合判断确定阳性样本 2 88份阳性 ,阴性样本 2份 ,4份样本结果不确定 .
短句来源
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  fusion antigens
     Construction of the attenuated Salmonella typhimurium strain expressing Escherichia coli LT-B/ST fusion antigens
     表达大肠杆菌LT-B/ST融合抗原的减毒鼠伤寒沙门氏菌株的构建
短句来源
     Construction of the attenuated Salmonella typhimurium strain expressing Escherichia coli LT B/ST fusion antigens
     表达大肠杆菌 LT-B/pro-ST融合抗原的减毒鼠伤寒沙门菌株的构建
短句来源
  “融合抗原”译为未确定词的双语例句
     Plasmodium falciparum chimeric protein 2 (PfCP-2), fused by erythrocytic stage antigens, AMA-1(III) and MSP1-19, is a potential vaccine candidate against malaria.
     由两个疟疾疫苗候选抗原AMA 1(III)和MSP1 19融合而成的恶性疟原虫融合抗原 2 (PfCP 2 ) ,是一个很有应用前景的疟疾疫苗候选抗原。
短句来源
     The Characters of Immune Response and Potency-stability Test of the Plasmodium Falciparum Chimeric Protein 2.9(PfCP-2.9)
     恶性疟原虫PfCP-2.9融合抗原免疫反应特性、效力和稳定性的研究
短句来源
     The band was further verified by western blotting as fussion: protein P30-CTA2/B. Conclusion: Prokaryotic express plasmid PUAB024 was successfully constructed and the fusion protein P30-CTA2/B was specifically expressed.
     Westernblotting进一步证实该条带为p30 CTA2 /B融合蛋白。 结论 :成功构建的表达载体pUAB0 2 4 p30可有效表达特异性的融合抗原蛋白P30 CTA2 /B。
短句来源
     Expression of HIV 1 Gag/Env chimera protein in E.Coli and its immunological analysis
     重组HIV-1 Gag/Env融合抗原在大肠杆菌中的表达及免疫学分析
短句来源
     BALB/c mice were vaccinated intramuscularly with pST-CE2t and pCE2t (only encoding HCV core-E2 fusion gene) . The serum antibodies, T lymphocyte proliferative response of the mice were detected. pST-CE2t could express -HCV core and E2 antigens in COS?
     将pST-CE2t和仅编码HCV核心-包膜E2融合抗原的质粒pCE2t分别肌肉注射接种BALB/c小鼠,检测小鼠的血清抗体、T细胞增殖反应。
短句来源
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  fusion antigen
The resulting GST-105 fusion antigen peptide was expressed with high efficiency in E.coli.
      
The intensity of ELISA reaction was highest against the recombinant fusion antigen GST-BP22; the chemically conjugated SAK-BP22 performed less well than the free dimeric form of the peptide.
      
Expression behaviour of the fusion antigen CTB::VP60, a promising RHD-vaccine, in pea
      
Construction of a polyepitope fusion antigen of human cytomegalovirus ppUL32 and detection of specific antibodies by ELISA.
      
This strongly demonstrates that the fusion antigen contains epitopes of both Sta56 and Sta47.
      
更多          
  chimeric antigen
Furthermore, with the development of efficient T-cell transduction methodologies, investigators are able to generate autologous antitumor T-cell responses through the introduction of chimeric antigen receptors able to target tumor antigens.
      
Here, the authors discuss the potential of lymphocytes grafted with chimeric antigen receptors in the immunotherapy of malignant disease.
      
Expression of a CD20-specific chimeric antigen receptor enhances cytotoxic activity of NK cells and overcomes NK-resistance of l
      
Expression of chimeric antigen receptors in effector cells operative in ADCC might allow to bypass insufficient activation via FcγRIII and other resistance mechanisms that limit natural killer (NK)-cell activity.
      
Here we have generated genetically modified NK cells carrying a chimeric antigen receptor that consists of a CD20-specific scFv antibody fragment, via a flexible hinge region connected to the CD3ζ chain as a signaling moiety.
      
更多          
  fusion antigens
The fusion vectors will also be useful for production and purification of LT-B fusion antigens to be used and evaluated in other vaccine compositions.
      


Four oligonucleotides which encode epitopes of structure and nonstructural antigen HCV, were chemically synthesized and fused with cholera toxin B subunit(CTB) gene in different ways of tanden repeat. Twelve recombinant plasmids were constructed and all of these plasmids could expressed fusion proteins of CTB and HCV epitopes. The expression level of fusion proteins ranged from 10~50 fig/ml depending on amino acid composition of the epitopes, and more than 95% of the chimeras were secreted into the medium. Purified...

Four oligonucleotides which encode epitopes of structure and nonstructural antigen HCV, were chemically synthesized and fused with cholera toxin B subunit(CTB) gene in different ways of tanden repeat. Twelve recombinant plasmids were constructed and all of these plasmids could expressed fusion proteins of CTB and HCV epitopes. The expression level of fusion proteins ranged from 10~50 fig/ml depending on amino acid composition of the epitopes, and more than 95% of the chimeras were secreted into the medium. Purified fusion proteins were obtained by affinity chromatography. The work described provided a sound basis for the application of the fusion protein in assambling anti-HCV ELISA kit.

通过固相化学合成法合成了编码丙型肝炎病毒(HCV)结构区和非结构区的4个抗原决定簇基因,这些抗原决定簇基因片段以不同方式串联后与ctxB基因融合,构建了12种表达不同嵌合蛋白的重组质粒,各重组质粒转化大肠杆菌后均能高效分泌性表达融合蛋白,表达产量在10~50μg/ml之间,随所融合的抗原决定簇不同而不同,表达水平主要与抗原决定簇的氨基酸组成有关,而与抗原决定簇的大小及串联次数关系不大。融合蛋白通过亲和层析纯化,达到了电泳纯,为进一步研究融合蛋白的抗原性及用作抗-HCV ELISA诊断试剂抗原打下了基础。

Four oligonucleotides,which encode epitopes of structural and nonstructural antigens of HCV,were chemically synthesized and fused with cholera toxin B subunit(CTB)gene in diferentways.Twelve recombinant plasmids were constructed and all of them could express fusion proteins consisting of CTB and HCV epitopes.The expression level of fusion proteins ranged from 10-50μg/ml depending on amino acid composition of the epitopes and more than 95%of thechimeras were secreted into medium.Purified fusion proteins were...

Four oligonucleotides,which encode epitopes of structural and nonstructural antigens of HCV,were chemically synthesized and fused with cholera toxin B subunit(CTB)gene in diferentways.Twelve recombinant plasmids were constructed and all of them could express fusion proteins consisting of CTB and HCV epitopes.The expression level of fusion proteins ranged from 10-50μg/ml depending on amino acid composition of the epitopes and more than 95%of thechimeras were secreted into medium.Purified fusion proteins were obtained by affinity chromatography.The reactivity of the 11 chimeras with human anti-HCV positive sera was evaluated in this work.The results demonstrated that most of the chimeras could react with anti-HCV antibodies in the sera.TheELISA kit showed high accordance with ABBOTT An-ti-HCV ELISA kit by using fusion protein 95082 as antigen.

通过固相化学合成法,合成了编码丙型肝炎病毒(HCV)结构区和非结构区的4个抗原决定簇基因,这些抗原决定簇基因片段以不同方式串朕后与ctxB基因融合,构建了12种表达不同嵌合蛋白的重组质粒。各重组质粒转化大肠杆菌后均能高效分泌性表达融合蛋白,表达产量随所融合的抗原决定簇不同在10~50μg/ml之间,表达水平主要与抗原决定簇的氨基酸组成有关,而与抗原决定簇的大小及串联次数关系不大。融合蛋白通过亲和层析纯化达到了电泳纯。对11种融合蛋白中HCV抗原决定簇的反应原性的研究表明,多数融合蛋白中HCV抗原决定簇均能与对应的HCV抗体结合。其中以融合蛋白95082为抗原研制的抗-HCVELISA试剂具有良好的灵敏度和特异性。

We have expressed a synthetic hybrid antigen gene

在减毒鼠伤寒沙门氏菌SL3261中表达了人工合成的恶性疟杂合45肽抗原基因,并在BALB/c小鼠及家兔中诱发出一定水平的特异性体液免疫及细胞免疫。在减毒菌中表达的融合抗原GZ-A可特异地识别兔抗GZ-A血清、鼠抗Pf血清及恶性疟患者血清,滴度分别为1:6400、1:10240及1:4。小鼠口服(po)重组疫苗图SL3261(nWRA)后,细菌在肠道至少可寄生1月以上,且在体外仍然可以表达目的抗原。静脉注射(iv)活菌后首日可见菌血症。10 ̄8cfu量全身免疫(ip、iv)后可致小鼠死亡。ip、iv组脾脏肿大1~2倍,肝脏轻度肿大,而po组则未见明显改变。清理切片显示肝脏轻度充血,汇管区有淋巴细胞聚集,枯否氏细胞轻度增生等;脾小体增生扩大,巨细胞明显增生等,上述改变以ip及iv组较为明显。结果说明,SL3261(pWRA)所表达的恶性疟原虫杂合抗原可特异地被抗Pf抗体所识别,活菌苗口服后可在肠道较长久地寄生,且不引起明显的毒副作用。

 
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