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   杂交蛋白 的翻译结果: 查询用时:0.458秒
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杂交蛋白
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  hybrid protein
     Studies on the Preliminary Purification of Human IFNγ/TNFβHybrid Protein and Its Anti-Tumor Effect
     人IFNγ/TNFβ杂交蛋白的初步纯化及其抗癌效应研究
短句来源
     Utilizing recombinant HuIFN-γ/TNF-β hybrid protein (γTNFβ) prepared from micro-pore glass absorbed chromatography to treals human tumor cells in ritro, the results reveals that this hybrid protein has obvious antitumor effects on all three cell lines detected (human cervical cancer ME180, stomach eancer 7901 and lung cancer G6), and its killing rates are higher than that of either IFN-γ or TNFβ alone at same dose.
     用MPG吸附层析法所制备的HuIFN-γ/HuTNFβ重组双功能杂交蛋白(简称γTNFβ)[1]去处理人宫颈癌细胞株ME180、胃癌细胞株7901和肺癌细胞株G6,结果表明此杂交蛋白对这三个细胞株都有明显的杀伤作用,其杀伤率显著高于同剂量的IFN-γ或TNF-β;
短句来源
     The hybrid protein has appreciable killing effect against human tumor cell lines(such asME 180,7901 as well as G_6)in vitro and its cytotoxic action is significantly stronger than that of single IFNγor TNFβ.
     该杂交蛋白在体外对人宫颈癌细胞株(ME180),人胃癌细胞株(7901)和人肺癌细胞株(G6)均有明显的杀伤作用,并都显著地高于单一的IFNγ或TNFβ对这些肿瘤细胞的杀伤。
短句来源
     PRELIMINARY PURIFICATION OF HUMAN IFNγ/ TNFβ HYBRID PROTEIN WITH MPG
     HuIFN-γ/HuTNF-β重组双功能杂交蛋白的分离和初步纯化
短句来源
     A recombinant hybrid protein──human interferon γ/tumor necrosis factor β(Hu IFNγ / TNFβ, γTNFβ) expressed by the extra fusion gene in E. coli HB 101 was identified and purified by means of micropore glass absorbed chromatography(MPG).
     本文对E.coliHB101菌株的外源基因表达产物"人γ-干扰素/人β-肿瘤坏死因子重组双功能杂交蛋白"(HuIFN-γ/HuTNF-β简称γTNFβ)进行了分离和初步纯化,并将产物作了抗病毒和细胞毒活性检测。
短句来源
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  “杂交蛋白”译为未确定词的双语例句
     In competition assays, recombinant Hu-IFN-αA displaced 70% of [γ-32P] -IFN-αA/γ binding to MDBK celis
     [γ-~(32)P]杂交蛋白与MDBK细胞的结合,可被αA型干扰素大大抑制(70%)。
短句来源
     Anti-serum of rat immunized with β-galactosidase-hCG was detected by counter current electrophoresis and immunodiffusion, the results hare clearly demonstrated that β-galactosidase hCG can be crossly immunoprecipitated with rat anti-β-galactosidase-hCG serum, whereas using hCG replaced β-galactosidase-hCG, the anti-serum produced against β-galactosidase-hCG has no cross reaction with hCG.
     而此杂交蛋白经解聚处理后,其抗血清与hCG相作用,在对流免疫电泳和免疫双扩散试验中都出现抗原抗体反应的免疫沉淀线。
短句来源
     A recombinant hybrid protein-human interferonγ/tumor necrosis facter β(γTNFβ)was identified andpurified by means of micro-pore glass absorbed chromatography. It possesses dual functional activities of an-ti-virus and anti-tumor. The antivial and cytotoxic activities are 1.7 × 10 ̄6u/mg protein and 1.4 × 10 ̄6u/mgprotein respectively.
     采用国产的微孔玻璃珠吸附层析法分离纯化人干扰素γ/肿瘤坏死因子β重组杂交蛋白,它具有抗病毒和抗肿瘤的双重功能活性,其抗病毒活性为1.7×10 ̄6u/mg蛋白,细胞毒活性为1.4×10 ̄6u/mg蛋白。
短句来源
     The proteins extracted from ectopic endometrium of ovarian endometriosis were transferred from two dimensional gel onto nitrocellulose membranes,followed by incubation with sera from women with and without endometriosis. Analyzed by MALDI-TOF-MS,the proteins hybridized differently were identified through their Peptide Mass Footprints.
     卵巢子宫内膜异位症(巧克力囊肿)组织总蛋白双向电泳后转膜,用患者血清和正常血清作为一抗行Westernblot检测,比较杂交蛋白点的差异,取差异点用MALDI-TOF-MS分析,根据其肽质量指纹图谱进行数据库查询,鉴定抗原。
短句来源
  相似匹配句对
     The Analysis of Hybrid Rice Seed Protein with Polyacrylamide Gel Electrophoresis
     杂交水稻种子蛋白电泳分析
短句来源
     Southwestern blot hybridization of DN A-binding proteins
     DNA结合蛋白的Southwestern印迹杂交
短句来源
     e-Hybrid Rice
     e杂交
短句来源
     proteinuria(-);
     尿蛋白(-);
短句来源
     northern hybridization.
     Northern杂交
短句来源
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  hybrid protein
Another hybrid protein, 12FN, containing 179 N-terminal amino acid residues of gp12 fused to the C-terminal domain (31 amino acids) of T4 fibritin, was shown to have a trimeric proteolytically resistant a-helical structure.
      
The hybrid protein consisting of Tte DNA polymerase fragment and mutant Taq DNA polymerase (F667Y) fragment in the ratio 20 : 1 was constructed.
      
The hybrid protein D-LD(1-72)-mCYP11Alp synthesized in yeast cells was imported into yeast mitochondria, underwent processing, and was inserted into the inner membrane on the side of the intermembrane space.
      
In the presence of adrenodoxin and adrenodoxin reductase, the hybrid protein exhibited cholesterol side-chain cleavage activity.
      
Thus, CYP11Alp insertion into the inner membrane of mitochondria mediated by the D-LD topogenic signal resulted in the catalytically active mCYP11Alp domain in the hybrid protein.
      
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We show that the fusion between the 3'-end coding sequences of Hu-IFN-aA gene and the 3'-end coding sequences for C-terminal 16 amino acids of Hu-IFN-y was formed by oligonucleotide directed mutagenesis.Under the control of phage A PL promoter,the hybrid protein Hu-IFN-αA/γ was expressed. This molecule has been purified by the use of monoclonal anti-body against Hu-IFN-α. The purified protein mainly exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and has anti-viral activity...

We show that the fusion between the 3'-end coding sequences of Hu-IFN-aA gene and the 3'-end coding sequences for C-terminal 16 amino acids of Hu-IFN-y was formed by oligonucleotide directed mutagenesis.Under the control of phage A PL promoter,the hybrid protein Hu-IFN-αA/γ was expressed. This molecule has been purified by the use of monoclonal anti-body against Hu-IFN-α. The purified protein mainly exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and has anti-viral activity on bovine MDBK celis, The hybrid Hu-IFN-αA/γ can be phosphorylated by [γ-32P] ATP and cAMP-dependent protein kinase. In competition assays, recombinant Hu-IFN-αA displaced 70% of [γ-32P] -IFN-αA/γ binding to MDBK celis

使用寡核苷酸指导的定点突变方法,将人αA型干扰素的完整基因与γ干扰素C端16个氨基酸的编码序列融合,在噬菌体λP_L启动子控制下,合成了一个杂交蛋白质。此蛋白质经抗人α干扰素单克隆抗体纯化后,在MDBK细胞上具有抗病毒活性,并像γ干扰素一样,可被依赖于cAMP的蛋白激酶磷酸化。[γ-~(32)P]杂交蛋白与MDBK细胞的结合,可被αA型干扰素大大抑制(70%)。

The present investigation has been earried out to study hCG B-subunit C-terminal containing hybrind protein synthesized by λgt11 hCG Y1089 through genetic engineering technology. A 36 peptide gene containing h CG B-subunit C-terminal has been artificially synthesized by Oligonucleotide Synthesizer, the double stranded DNA was cloned into an expression vetor λgt11 and the 36 peptide gene fusion product was successfully expressed in host strain E. coli Y1089. h CG B-subunit C-terminal 36 peptide containing hybrid...

The present investigation has been earried out to study hCG B-subunit C-terminal containing hybrind protein synthesized by λgt11 hCG Y1089 through genetic engineering technology. A 36 peptide gene containing h CG B-subunit C-terminal has been artificially synthesized by Oligonucleotide Synthesizer, the double stranded DNA was cloned into an expression vetor λgt11 and the 36 peptide gene fusion product was successfully expressed in host strain E. coli Y1089. h CG B-subunit C-terminal 36 peptide containing hybrid protein (B-galactosidase-h CG) expressed in λgt11 hCG Y1089 was precipitated in the ammonium sulfate solution of 50% saturation, affinity isolated by anti-B-galactosidase sepharose 4B column and finally purified with preparative polyacrylamide gel electrophoresis. Comparative analysis of different preparations revealed that the eluate of affinity column gave a mjor band and two minor bands, and the eluate of preparative polyacrylamide gel electrophoresis only gave one band while the solution of ammonium sulfate precipitated gave more than 30 bands in polyacrylamide gel electrophoresis, it indicated that anti-B-galactosidase affinity chromatography and preparative polyacrylamide gel eleetrophoresis can be used for isolating and purifing B-galactosidase-h CG. The results of western blot technique demonstrated that B-galactosidase-hCG contains immunological property of hCG B-subunit C-terminal. Preliminary studies of antigenicitY of this B-galactosidase-hCG through counter current immunoelectrophoresis and double immunodiffusion techniques have clearly demonstrated that this Purified B-galactosidase-hCG can be crossly immunopreciPitated with rabbit anti-B-galactosidase-hCG serum, it has been concluded that B-galactosidase-hCG possesses the antigenieitY, but the biological activity of this this hybrid protein to neutralizing hCG has to be determined by in vitro or in vivo system.

通过寡核苷酸合成仪,用化学方法合成相当于hcGβ链C末端36肽基因的片段,成功地克隆到噬菌体载体λgt11中,并在宿主菌株E. coli y_(1089)中得到表达。含λgt11 hCG重组体的E. coli y_(1089)所合成的含人类绒毛膜促性腺激素β链c末36肽的杂交蛋白(下称:β—galactosidase—hCG),通过硫酸铵沉淀、亲和层析和制备性聚丙烯酰胺凝胶电泳,可得电泳纯单一色带的β—gaIactosidase—hCG。蛋白质转印(Western Blot)技术证实β—galactasidase—hCG具有hCGβ链c末端36肽的免疫特性。对流免疫电泳和免疫双扩散试验结果表明β—galactosidase—hCG具免疫原性。从一立升培养液的菌体中可分离纯化约3.8毫克的β—galaetosidase—hCG。

An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E.Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG). Anti-serum of rat immunized with β-galactosidase-hCG...

An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E.Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG). Anti-serum of rat immunized with β-galactosidase-hCG was detected by counter current electrophoresis and immunodiffusion, the results hare clearly demonstrated that β-galactosidase hCG can be crossly immunoprecipitated with rat anti-β-galactosidase-hCG serum, whereas using hCG replaced β-galactosidase-hCG, the anti-serum produced against β-galactosidase-hCG has no cross reaction with hCG. When β-galactosidase-hCG was treated with 2M Urea, 0.9M SDS and 0.005M Mercaptoethanol solvent system before using as an antigen to immunize rat, the anti-body against this depolymerized antigen can be crossly immunoprecipitated with hCG. It revealed that the β-galactosidase-hCG polymer was depolymerized and the conformation of β-galactosidase-hCG subunit has been changed, therefore the antigenic determinant of hCG-β-subunit 36C-terminal peptide in β-galactosidase-hCG appeared its immunogenicity in Urea and SDS solvent. the depolymerized optimal condictions for, β-galaztosidase-hCG in Urea and SDS solvent system were 60C in 40 minutes.

基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位C末端36肽的β-半乳糖苷酶杂交蛋白(简称β-galaclosidase-hCG),在未经解聚处理前免疫新西兰种白兔和昆明种小白鼠,其抗血清在免疫双扩散试验和对流免疫电泳中对hCG没有形成沉淀线作用。而此杂交蛋白经解聚处理后,其抗血清与hCG相作用,在对流免疫电泳和免疫双扩散试验中都出现抗原抗体反应的免疫沉淀线。从多种蛋白质解聚系统中,经过反复试验,筛选出对β-galactosidase-hCG杂交蛋白有良好解聚作用的解聚剂为2M脲、0.9MSDS、0.005M巯基乙醇;解聚作用最适温度是60℃,最适时间为40分钟。

 
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